FastQCFastQC Report
Thu 26 May 2016
SRR1364109_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364109_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1441969
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119760.8305310308335337No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103790.7197796901320348No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67050.46498919186196097No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30080.2086036523670065No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23570.16345705074103534No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15890.11019654375371453No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52400.076.107291
GTATCAA96400.054.6646581
TGGTATC19850.044.05692
GTGGTAT20800.042.98921
TATCAAC128600.040.941092
ATCAACG130350.040.3928383
TCAACGC131100.040.117774
CAACGCA132400.039.76885
AACGCAG136900.038.723676
ACGCAGA156600.033.7050067
CGCAGAG157800.033.3732728
TAAGGTG10600.032.5543755
GTACATG83750.032.1013371
TACATGG83750.031.8236712
GGTAAGG10850.030.7065093
ACATGGG83050.030.6602943
GCAGAGT170250.030.4783829
AGGTAAG10650.029.047642
GAGTACT127250.028.52410512-13
GTAAGGT11750.028.3555074
Produced by FastQC (version 0.11.5)