##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364109_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1441969 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.0966671266858 33.0 31.0 34.0 30.0 34.0 2 32.106823378311184 33.0 31.0 34.0 30.0 34.0 3 32.24282768908347 34.0 31.0 34.0 30.0 34.0 4 35.895721752686775 37.0 35.0 37.0 35.0 37.0 5 35.83309211224375 37.0 35.0 37.0 35.0 37.0 6 35.875840604062915 37.0 35.0 37.0 35.0 37.0 7 35.80521495260994 37.0 35.0 37.0 33.0 37.0 8 35.7778433516948 37.0 35.0 37.0 33.0 37.0 9 37.40717102794859 39.0 37.0 39.0 34.0 39.0 10-11 37.351229464711096 39.0 37.0 39.0 34.0 39.0 12-13 37.2892659273535 39.0 37.0 39.0 33.0 39.0 14-15 38.55000419565192 40.0 38.0 41.0 34.0 41.0 16-17 38.552901275963634 40.0 38.0 41.0 34.0 41.0 18-19 38.7446474230722 40.0 38.0 41.0 34.0 41.0 20-21 38.817930205156976 40.0 38.0 41.0 34.0 41.0 22-23 38.78235697161312 40.0 38.0 41.0 34.0 41.0 24-25 38.733822641124746 40.0 38.0 41.0 34.0 41.0 26-27 38.59204774859931 40.0 38.0 41.0 34.0 41.0 28-29 38.48642931990909 40.0 38.0 41.0 34.0 41.0 30-31 38.239465966327984 40.0 37.5 41.0 34.0 41.0 32-33 37.976954775033306 40.0 36.5 41.0 33.0 41.0 34-35 37.787869572785546 40.0 36.0 41.0 33.0 41.0 36-37 37.60552654044574 40.0 36.0 41.0 33.0 41.0 38-39 37.44289960463782 40.0 36.0 41.0 33.0 41.0 40-41 37.233330952329766 40.0 36.0 41.0 32.0 41.0 42-43 36.996986759077345 40.0 35.0 41.0 31.5 41.0 44-45 36.691878604879854 40.0 35.0 41.0 31.0 41.0 46-47 36.196028486049286 40.0 35.0 41.0 29.5 41.0 48-49 35.71265436358202 40.0 35.0 41.0 27.5 41.0 50-51 35.44906721295673 39.0 35.0 41.0 26.0 41.0 52-53 35.257898748170035 39.0 35.0 41.0 26.0 41.0 54-55 35.07011801224576 39.0 35.0 41.0 25.5 41.0 56-57 34.862044190963886 39.0 35.0 41.0 25.0 41.0 58-59 34.631027435402565 38.0 34.5 41.0 24.5 41.0 60-61 34.3894712715738 38.0 34.0 40.0 24.0 41.0 62-63 34.12407513615064 37.0 34.0 40.0 23.0 41.0 64-65 33.89374806254503 37.0 34.0 40.0 23.0 41.0 66-67 33.68061622683983 36.5 34.0 40.0 23.0 41.0 68-69 33.37080235428085 36.0 34.0 39.0 22.0 41.0 70-71 33.03746821186863 35.5 33.5 39.0 22.0 41.0 72-73 32.728322522883644 35.0 33.0 38.5 21.5 40.5 74-75 32.46236014782565 35.0 33.0 37.0 21.5 39.5 76-77 32.273827315289026 35.0 33.0 37.0 21.5 39.0 78-79 31.98657183337506 35.0 33.0 36.5 20.0 39.0 80-81 31.73368047440687 35.0 33.0 36.0 20.0 38.5 82-83 31.476670788345658 35.0 33.0 36.0 19.5 37.0 84-85 31.27050650880844 35.0 33.0 35.0 19.0 37.0 86-87 31.092521753241574 35.0 33.0 35.0 18.0 36.5 88-89 30.9326407155771 35.0 33.0 35.0 17.5 36.0 90-91 30.79669119100341 35.0 33.0 35.0 16.0 36.0 92-93 30.681794130109594 35.0 33.0 35.0 13.5 36.0 94-95 30.582299966226735 35.0 33.0 35.0 10.5 35.5 96-97 30.509504711959828 35.0 33.0 35.0 8.5 35.0 98-99 30.436087391615214 35.0 32.0 35.0 7.0 35.0 100-101 30.36132815615315 35.0 32.0 35.0 4.5 35.0 102-103 29.18180661304092 33.5 30.0 34.5 2.0 35.0 104-105 30.077353257941052 35.0 32.0 35.0 2.0 35.0 106-107 30.207174356730278 35.0 32.0 35.0 2.0 35.0 108-109 30.19481694821456 35.0 32.0 35.0 2.0 35.0 110-111 30.13304204181921 35.0 32.0 35.0 2.0 35.0 112-113 30.054413444394434 35.0 32.0 35.0 2.0 35.0 114-115 29.96533593995433 35.0 32.0 35.0 2.0 35.0 116-117 29.885091843167224 35.0 32.0 35.0 2.0 35.0 118-119 29.772962178798572 35.0 32.0 35.0 2.0 35.0 120-121 29.63882163902275 35.0 32.0 35.0 2.0 35.0 122-123 29.488421040951643 35.0 32.0 35.0 2.0 35.0 124-125 28.680823928947156 34.5 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2631155259012772 1101 2 0.3522248405406856 1101 3 0.25656889789493675 1101 4 0.17376390048590906 1101 5 0.19553914665144134 1101 6 0.18770081415040352 1101 7 0.20168467262958245 1101 8 0.19897067307229577 1101 9 0.1718859736582985 1101 10-11 0.29430453204356155 1101 12-13 0.30114268261853994 1101 14-15 0.38109431314643416 1101 16-17 0.3937457601224281 1101 18-19 0.38927458160799944 1101 20-21 0.4370504058445377 1101 22-23 0.3172624947510698 1101 24-25 0.367406050505771 1101 26-27 0.378740122822002 1101 28-29 0.46176820851945877 1101 30-31 0.5055348357838909 1101 32-33 0.543391528056894 1101 34-35 0.49026409479548505 1101 36-37 0.538746295028389 1101 38-39 0.5829625510452416 1101 40-41 0.43272739889007994 1101 42-43 0.45563358706106527 1101 44-45 0.5915017683531119 1101 46-47 0.7427358264576185 1101 48-49 0.6426671107938944 1101 50-51 0.6999012353529963 1101 52-53 0.7298070441098403 1101 54-55 0.7660126904356304 1101 56-57 0.7666564481879234 1101 58-59 0.7491189966089564 1101 60-61 0.7178775740249534 1101 62-63 0.73599335026028 1101 64-65 0.7090839162479696 1101 66-67 0.747407348336246 1101 68-69 0.7394927009055579 1101 70-71 0.7099469675317991 1101 72-73 0.6991436714372199 1101 74-75 0.6188225939160077 1101 76-77 0.6011875860629061 1101 78-79 0.5604470107098258 1101 80-81 0.6284477965087838 1101 82-83 0.5375166409781116 1101 84-85 0.516943463480672 1101 86-87 0.5471224772889265 1101 88-89 0.5287879987787569 1101 90-91 0.5563752520697491 1101 92-93 0.6139353399410368 1101 94-95 0.6718720620728362 1101 96-97 0.6979020462658596 1101 98-99 0.6846606219389706 1101 100-101 0.6606364005181824 1101 102-103 0.8152431889443115 1101 104-105 0.7653367763804724 1101 106-107 0.8120769353999258 1101 108-109 0.7923471740243606 1101 110-111 0.7291200017657467 1101 112-113 0.7643938098527592 1101 114-115 0.8050890805247626 1101 116-117 0.7032438792604729 1101 118-119 0.657240061166803 1101 120-121 0.8070853732038046 1101 122-123 0.6721092657265224 1101 124-125 0.7623621819865214 1102 1 0.2117936476825264 1102 2 0.23927473508848607 1102 3 0.12424572368941256 1102 4 0.12290986028956752 1102 5 0.16440763889249865 1102 6 0.16573954472475094 1102 7 0.16585096829567192 1102 8 0.16022170590018447 1102 9 0.2133386861756108 1102 10-11 0.313691867026904 1102 12-13 0.2702467445089738 1102 14-15 0.23761223530422626 1102 16-17 0.323506860900018 1102 18-19 0.3023818042347628 1102 20-21 0.33968527241638213 1102 22-23 0.23797067040914754 1102 24-25 0.2277967235511298 1102 26-27 0.30943536690895 1102 28-29 0.3637890376868995 1102 30-31 0.2843416554662852 1102 32-33 0.377819195205241 1102 34-35 0.31643927844077524 1102 36-37 0.2317445708189041 1102 38-39 0.22985850235990313 1102 40-41 0.26936880764846194 1102 42-43 0.2700884142816946 1102 44-45 0.2739116718049246 1102 46-47 0.22423429466537925 1102 48-49 0.2795967445597256 1102 50-51 0.23957597474080927 1102 52-53 0.26184062563627464 1102 54-55 0.18389945382655526 1102 56-57 0.1720507101923019 1102 58-59 0.24362390419208424 1102 60-61 0.31204683612365613 1102 62-63 0.27016556935939207 1102 64-65 0.27445696990974255 1102 66-67 0.26265500065713354 1102 68-69 0.20179849153294782 1102 70-71 0.17731514756642497 1102 72-73 0.23627112239910986 1102 74-75 0.28267814515370304 1102 76-77 0.24497456035822296 1102 78-79 0.2210374817170795 1102 80-81 0.19941887166847394 1102 82-83 0.21523319055950907 1102 84-85 0.23462361413206168 1102 86-87 0.25976546971673997 1102 88-89 0.20427559729827394 1102 90-91 0.3120297489740942 1102 92-93 0.2829605720679247 1102 94-95 0.2642081725656169 1102 96-97 0.3113592513486907 1102 98-99 0.26153520456331236 1102 100-101 0.275653694490245 1102 102-103 0.3700780968292676 1102 104-105 0.35278829477895357 1102 106-107 0.3228153979106274 1102 108-109 0.34979763601440794 1102 110-111 0.31971268462816127 1102 112-113 0.38807424252747325 1102 114-115 0.2857208457832634 1102 116-117 0.2927577521669882 1102 118-119 0.26223043558813686 1102 120-121 0.29535216538185693 1102 122-123 0.40920610914038136 1102 124-125 0.514810196315949 1103 1 0.24354160779630263 1103 2 0.28582811439933664 1103 3 0.2557689668614245 1103 4 0.17774907625577185 1103 5 0.1777849546987298 1103 6 0.2453063182818198 1103 7 0.25915562974227413 1103 8 0.25422806441499546 1103 9 0.3287107709411643 1103 10-11 0.2431969360846935 1103 12-13 0.3159092179236538 1103 14-15 0.3226991249398736 1103 16-17 0.3183975361764908 1103 18-19 0.3144741244154474 1103 20-21 0.35001385516676464 1103 22-23 0.42019764687496064 1103 24-25 0.3631789574141564 1103 26-27 0.42413080704482553 1103 28-29 0.3565950984094286 1103 30-31 0.316981779817894 1103 32-33 0.44364384826899794 1103 34-35 0.3639893787420405 1103 36-37 0.454675125001728 1103 38-39 0.5013047624769698 1103 40-41 0.4125672879378044 1103 42-43 0.4540975457239469 1103 44-45 0.5071009135785687 1103 46-47 0.5493085597023324 1103 48-49 0.5109995987531661 1103 50-51 0.561968599302908 1103 52-53 0.653513538253371 1103 54-55 0.6770871352565422 1103 56-57 0.6178739539134099 1103 58-59 0.5883069073365661 1103 60-61 0.5251889793128726 1103 62-63 0.5540481345094648 1103 64-65 0.47576212188080547 1103 66-67 0.4533248776965664 1103 68-69 0.4646823086185776 1103 70-71 0.40446286223494354 1103 72-73 0.42023026335900227 1103 74-75 0.4903868834442662 1103 76-77 0.453516664012632 1103 78-79 0.4994143445203143 1103 80-81 0.4064483342513405 1103 82-83 0.4092956711124103 1103 84-85 0.397465265332567 1103 86-87 0.3465578698822007 1103 88-89 0.4116601410190128 1103 90-91 0.41744320343768493 1103 92-93 0.4248064494660966 1103 94-95 0.4953949351774014 1103 96-97 0.4742015889173743 1103 98-99 0.429871004246305 1103 100-101 0.41319741614323746 1103 102-103 0.48989624586817015 1103 104-105 0.6216032480876166 1103 106-107 0.5687650248924037 1103 108-109 0.5547721122697311 1103 110-111 0.5284235204598708 1103 112-113 0.6385716306677622 1103 114-115 0.6042378924233525 1103 116-117 0.6250933349576364 1103 118-119 0.6302058004614608 1103 120-121 0.6338245660598041 1103 122-123 0.6503564210260784 1103 124-125 0.6907434053708847 1104 1 0.12741615411874818 1104 2 0.13788078588567032 1104 3 0.09138902174274222 1104 4 0.0784369149861277 1104 5 0.16865140216013685 1104 6 0.17699445657491708 1104 7 0.17821057594987622 1104 8 0.1975687045903598 1104 9 0.15323344837430852 1104 10-11 0.12898230604468353 1104 12-13 0.1428896210088837 1104 14-15 0.1469580182644279 1104 16-17 0.26330188054741654 1104 18-19 0.15681178496374315 1104 20-21 0.0974600160968464 1104 22-23 0.1876397321516734 1104 24-25 0.25282175328570133 1104 26-27 0.17027615897772108 1104 28-29 0.17022873672786432 1104 30-31 0.18028994365826634 1104 32-33 0.10970227811576905 1104 34-35 0.04161608622746371 1104 36-37 0.02444191051569078 1104 38-39 0.09593436692080815 1104 40-41 0.025459219566386082 1104 42-43 0.21284842241164625 1104 44-45 0.12993141706621714 1104 46-47 0.2657714214776803 1104 48-49 0.3721455995905245 1104 50-51 0.24274327122836326 1104 52-53 0.269955999539107 1104 54-55 0.36269503429056726 1104 56-57 0.19963658288316566 1104 58-59 0.23000575255328926 1104 60-61 0.22809607130530196 1104 62-63 0.29602994207284183 1104 64-65 0.1959021830200527 1104 66-67 0.11486853252205975 1104 68-69 0.09289938582843149 1104 70-71 0.040809467886809614 1104 72-73 0.08861686019288584 1104 74-75 0.042364464239533106 1104 76-77 0.029282704619824074 1104 78-79 0.10549655716008388 1104 80-81 0.050370566162687425 1104 82-83 0.15529779738566418 1104 84-85 0.17971392818936138 1104 86-87 0.1098684115574251 1104 88-89 0.17223095027398827 1104 90-91 0.2146626700382157 1104 92-93 0.20877893147233095 1104 94-95 0.25462771231961057 1104 96-97 0.31851107456725103 1104 98-99 0.24453333391999266 1104 100-101 0.19873760244104233 1104 102-103 0.17125637628907953 1104 104-105 0.2209164093812639 1104 106-107 0.3033284321919574 1104 108-109 0.20025438162197062 1104 110-111 0.1770130866094597 1104 112-113 0.23841524913682832 1104 114-115 0.264169397446139 1104 116-117 0.2784484599250767 1104 118-119 0.3496900680838806 1104 120-121 0.40650488816531194 1104 122-123 0.3666042776413043 1104 124-125 0.48796298308519326 1105 1 0.09853275604613287 1105 2 0.1266418093689552 1105 3 0.01799257445914293 1105 4 0.01636481971620185 1105 5 0.04589787345845053 1105 6 -0.01743613908124786 1105 7 0.0492725740834814 1105 8 0.01802220827291734 1105 9 0.0714898341875525 1105 10-11 0.08623589044589863 1105 12-13 0.02187259339104486 1105 14-15 -0.03019431171049547 1105 16-17 0.09272186590118281 1105 18-19 0.07139633957758207 1105 20-21 0.12831481200831973 1105 22-23 0.1176735992873077 1105 24-25 0.07395118436530623 1105 26-27 0.040082406820509675 1105 28-29 0.21278979162202205 1105 30-31 0.18916557119100474 1105 32-33 0.2619258760857406 1105 34-35 0.247092992840372 1105 36-37 0.28215857709103886 1105 38-39 0.254162099438922 1105 40-41 0.1225998709038123 1105 42-43 0.13812203490002872 1105 44-45 0.19190900576998615 1105 46-47 0.1700360416891229 1105 48-49 0.21444199044411505 1105 50-51 0.1515610990931009 1105 52-53 0.23983009122600407 1105 54-55 0.28848797633931866 1105 56-57 0.19646171012462332 1105 58-59 0.020237015142754444 1105 60-61 -0.0568941858438734 1105 62-63 0.03367012681263759 1105 64-65 0.19198426950588754 1105 66-67 0.16924131024808986 1105 68-69 0.07165960577762576 1105 70-71 0.06029562609901973 1105 72-73 0.09116328711419186 1105 74-75 0.13213177720327707 1105 76-77 0.08652902982102972 1105 78-79 0.10755355877263284 1105 80-81 0.11241503994829927 1105 82-83 0.08520405782932627 1105 84-85 0.1069253818373177 1105 86-87 0.06859826157048232 1105 88-89 0.11790725204486918 1105 90-91 0.042952229025836175 1105 92-93 0.04458381695959446 1105 94-95 0.05753335550298999 1105 96-97 0.06482645987097868 1105 98-99 0.07026482387307809 1105 100-101 0.08355464320761286 1105 102-103 0.028324097776859958 1105 104-105 -0.017470468541819884 1105 106-107 2.7052842977326463E-4 1105 108-109 -0.031779554909793006 1105 110-111 0.061588610595865845 1105 112-113 -0.009047455011049976 1105 114-115 0.06762701376226588 1105 116-117 0.08961406830082552 1105 118-119 -5.213618232922101E-4 1105 120-121 0.009559048189125008 1105 122-123 0.031916806746590254 1105 124-125 -0.07911841674424025 1106 1 0.0526266600476788 1106 2 0.07094925336368618 1106 3 0.007883704278711434 1106 4 -0.009800870464147238 1106 5 3.4459952968290963E-4 1106 6 0.0204819859239862 1106 7 0.07029336480159998 1106 8 0.050411979474091595 1106 9 0.1597321544124739 1106 10-11 0.20147317595938574 1106 12-13 0.11030443702630066 1106 14-15 0.08528902367233115 1106 16-17 0.10338233735609492 1106 18-19 0.18161665393080995 1106 20-21 0.20711523019392075 1106 22-23 0.1357982078443385 1106 24-25 0.13327333405442232 1106 26-27 0.1312985917671483 1106 28-29 0.175008079397017 1106 30-31 0.20207520069918417 1106 32-33 0.12671106011517708 1106 34-35 0.19508820979839214 1106 36-37 0.21119836775142176 1106 38-39 0.24410358518786524 1106 40-41 0.22272255425558996 1106 42-43 0.1923640756881113 1106 44-45 0.14840864501437068 1106 46-47 0.2397581783319751 1106 48-49 0.19924485744848397 1106 50-51 0.17244790116762942 1106 52-53 0.24128181851150998 1106 54-55 0.1509464299428842 1106 56-57 0.17469108986738746 1106 58-59 0.23677081708373748 1106 60-61 0.23358294595291795 1106 62-63 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2216 68-69 -0.984375916590281 2216 70-71 -0.943265936679353 2216 72-73 -0.797649761122603 2216 74-75 -0.7505330864389812 2216 76-77 -0.6242562612730822 2216 78-79 -0.6621658147353422 2216 80-81 -0.7439288296079241 2216 82-83 -0.7454737807281688 2216 84-85 -0.6760781652541006 2216 86-87 -0.6502966195338864 2216 88-89 -0.6995370626932989 2216 90-91 -0.6749419842520119 2216 92-93 -0.6687135952394456 2216 94-95 -0.6710651092463813 2216 96-97 -0.8352026972419111 2216 98-99 -0.8749969844896519 2216 100-101 -0.9127851743674817 2216 102-103 -1.0582443887438728 2216 104-105 -0.8930591045570715 2216 106-107 -1.0453413260805213 2216 108-109 -1.042307470165504 2216 110-111 -1.1133036849586375 2216 112-113 -1.0754811863604168 2216 114-115 -1.1737684220776181 2216 116-117 -1.1483312878393725 2216 118-119 -1.3057773066048668 2216 120-121 -1.5076114898346447 2216 122-123 -1.4668156092096432 2216 124-125 -1.7969547445773841 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 10.0 4 38.0 5 152.0 6 425.0 7 1003.0 8 1832.0 9 3343.0 10 5744.0 11 8136.0 12 10171.0 13 15204.0 14 22917.0 15 21301.0 16 8751.0 17 6437.0 18 5936.0 19 5571.0 20 5374.0 21 5347.0 22 5648.0 23 6211.0 24 7402.0 25 9147.0 26 11534.0 27 14351.0 28 18201.0 29 22362.0 30 28302.0 31 35490.0 32 45383.0 33 58994.0 34 79984.0 35 119415.0 36 217179.0 37 427889.0 38 205418.0 39 1364.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.01480626816051 18.68472547449175 13.892885002914019 22.407583254433717 2 15.982726025668232 20.966574152286714 39.2618129212213 23.78888690082375 3 19.041463443388867 26.203406591958633 30.54864563662603 24.20648432802647 4 12.405745199792783 16.96014269377497 36.82541025500548 33.80870185142676 5 11.970645693492717 38.993001929999885 34.17077620947468 14.865576167032717 6 30.91987414431239 37.728827734854214 16.49383585916202 14.857462261671367 7 26.573872253841795 34.767460326817016 20.960159337683404 17.69850808165779 8 27.072634709900147 33.96147906092295 19.4470200122194 19.518866216957505 9 27.156345681067158 14.988435919041033 19.992926319293463 37.86229208059835 10-11 24.542854240069182 26.948775055679285 27.04770266376368 21.46066804048785 12-13 26.353620357950835 24.76998683657518 28.028905438339276 20.847487367134715 14-15 22.757493399650063 25.49205288047108 27.401421251080986 24.349032468797873 16-17 20.653075462460176 28.770314335606624 28.442878522457598 22.133731679475606 18-19 20.69496639664237 25.38459564664705 33.04041210317282 20.880025853537767 20-21 21.29140779032004 24.571679418905674 33.8487859309042 20.288126859870083 22-23 20.918410867362613 24.8468240301976 33.59448087996344 20.64028422247635 24-25 20.70252550505593 25.1437790964993 33.73297206805417 20.420723330390597 26-27 20.205219390985523 25.33653636104521 33.58335026619851 20.87489398177076 28-29 20.492465510700995 25.561055750851786 33.50023474845853 20.446243989988687 30-31 20.465800582398096 25.09773094983318 33.42030931316831 21.016159154600413 32-33 20.100189393808048 25.16739264158938 33.48026205833828 21.25215590626428 34-35 21.080203527260295 25.219994327201206 33.089893055953354 20.609909089585145 36-37 21.37937778135314 24.714574307769446 32.796232096529124 21.1098158143483 38-39 20.83633559389973 25.060732928377792 32.73992020632899 21.36301127139349 40-41 21.11470496245065 25.135526491901004 32.800635797302164 20.949132748346187 42-43 21.025209279811147 25.051197355837747 32.29098545114354 21.632607913207565 44-45 21.087936009720043 25.228454980654924 32.37666690476702 21.30694210485801 46-47 21.911601428324744 25.669206480860545 30.83200817770701 21.5871839131077 48-49 21.680389800335515 26.229516723313743 29.918049555850367 22.17204392050037 50-51 22.079704903503472 26.46589489787922 29.552230318404902 21.902169880212405 52-53 22.199506369415708 26.16359991095509 29.504760504560085 22.132133215069118 54-55 21.625360878077128 26.45414013754803 29.597376920030875 22.323122064343963 56-57 21.96260807271169 26.719125029733647 28.98082413699601 22.337442760558655 58-59 21.684862850727026 25.976078542603897 29.454863454068708 22.884195152600366 60-61 21.373205665309033 26.317452039537603 29.79048093266915 22.518861362484213 62-63 21.653204749894066 26.14071453685898 30.09905206006509 22.107028653181864 64-65 21.449178172346283 26.289018695963644 29.793705689928146 22.468097441761927 66-67 21.465128584595092 26.56298436374152 29.505523350363287 22.466363701300097 68-69 22.100821862328523 25.992618426609727 29.693842239326923 22.21271747173483 70-71 21.75289482644911 26.09418094286354 29.42122888910927 22.731695341578078 72-73 21.688191632413734 26.45237172227697 29.185232137445393 22.674204507863898 74-75 21.59571391617989 26.923983802703113 28.744168563956645 22.736133717160357 76-77 21.634896450617177 26.543219722476696 29.21449767644103 22.607386150465093 78-79 21.717734569883262 26.651335777676223 28.879018897077536 22.75191075536298 80-81 21.82883265867713 26.763196712273285 28.560426749812233 22.847543879237346 82-83 21.755980884471164 26.855084956750108 28.568991427693664 22.819942731085067 84-85 21.56207935122045 26.913026562984367 28.42491759531585 23.09997649047934 86-87 21.760592634099623 26.854634184230036 28.12501517022904 23.2597580114413 88-89 21.451293335709714 26.97544121961013 28.082330480058864 23.490934964621292 90-91 21.42646617229635 27.085915161837736 28.040131237217995 23.44748742864791 92-93 21.55205833135109 26.645614434152193 28.348702364613942 23.453624869882777 94-95 21.262671130039067 26.38036171624852 28.596998943797985 23.75996820991443 96-97 21.620482909524686 26.045558418320464 28.456326267351418 23.877632404803435 98-99 21.143196764911693 26.465994747350617 28.049764934577677 24.341043553160016 100-101 21.183090034877132 26.290727842548716 28.40482024302336 24.121361879550793 102-103 21.034672998988498 26.013955751190522 28.472631352275044 24.478739897545935 104-105 20.801531681086573 26.399824665378247 28.119844820536898 24.67879883299828 106-107 21.077264123264136 26.43802493832007 27.666351995571947 24.818358942843847 108-109 21.07689917189349 26.33975951753674 27.661102023998303 24.922239286571468 110-111 21.121860408337657 26.529324125991504 27.80600354569592 24.54281191997492 112-113 20.950669603606734 26.813312509776217 27.77354069489778 24.462477191719273 114-115 20.678161625179445 27.03687633468219 27.491687121532948 24.793274918605412 116-117 20.758699720726582 26.94953794331013 27.509923015986427 24.781839319976857 118-119 20.43548027434439 27.174340885768792 27.226152398675374 25.164026441211444 120-121 20.332311709048987 27.426536867087453 27.33667508636754 24.904476337496014 122-123 20.340498373872652 27.54525863713258 27.069592979180435 25.044650009814333 124-125 20.20847523225183 27.510934597719167 27.077642926601904 25.202947243427104 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1775.0 1 1175.0 2 779.0 3 973.0 4 646.5 5 525.0 6 1184.5 7 2613.5 8 4143.5 9 4554.5 10 3449.5 11 2229.5 12 1944.0 13 1951.5 14 2050.0 15 2220.0 16 2420.0 17 2655.0 18 2925.5 19 3177.5 20 3474.5 21 3902.5 22 4501.5 23 5315.5 24 6193.0 25 7575.5 26 9792.5 27 12304.0 28 14552.5 29 17110.0 30 19964.5 31 23819.0 32 30209.5 33 32236.5 34 30823.0 35 32554.5 36 35942.5 37 39145.5 38 40714.0 39 42476.5 40 43689.5 41 43860.0 42 43652.0 43 43807.0 44 44972.5 45 46942.0 46 47425.5 47 47399.0 48 47907.0 49 47773.5 50 47689.0 51 46525.5 52 45215.0 53 45257.5 54 46519.0 55 44367.0 56 40507.0 57 37297.0 58 34506.0 59 30508.0 60 24548.0 61 20850.5 62 18037.5 63 12795.5 64 8131.0 65 6290.5 66 5052.5 67 4210.0 68 3211.5 69 2170.0 70 1583.0 71 1091.0 72 806.0 73 718.0 74 538.0 75 388.5 76 273.5 77 165.5 78 95.0 79 34.5 80 16.0 81 11.0 82 7.5 83 5.5 84 2.0 85 1.5 86 1.5 87 1.0 88 1.0 89 0.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16477469349202375 2 0.0021498381726652934 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 2.773984738922959E-4 10-11 3.8142290160190687E-4 12-13 0.006588213754942028 14-15 0.0 16-17 4.854473293115178E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 6.934961847307398E-4 96-97 0.005825367951738213 98-99 0.002080488554192219 100-101 0.0034674809236536984 102-103 0.0037795542067825313 104-105 0.010714516054089927 106-107 0.01747610385521464 108-109 0.025451309979618145 110-111 0.015326265682549347 112-113 0.023058748142297095 114-115 0.026387529829004646 116-117 0.025728708453510444 118-119 0.014181996977743626 120-121 0.021533056535889467 122-123 0.013869923694614794 124-125 0.014910167971710905 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1441969.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.0693778363316 #Duplication Level Percentage of deduplicated Percentage of total 1 77.93886717929995 40.58228323294633 2 11.674277413102537 12.157447231779758 3 4.070329004915721 6.358184966254088 4 1.9919621959269989 4.1488092886164685 5 1.107317495546548 2.882866653019682 6 0.7066607030919605 2.207722989082982 7 0.4601410531778213 1.677148084914647 8 0.33821348535291834 1.4088452606546966 9 0.24312759382466914 1.1393552290765372 >10 1.3059175354027661 12.538125146744397 >50 0.1100133544836561 3.945451249189786 >100 0.04767233216343689 4.397570819322035 >500 0.0034051665830230546 1.2876427784217077 >1k 0.0013096794550088672 1.3051004921848661 >5k 2.619358910017734E-4 0.9144841467341966 >10k+ 5.238717820035469E-4 3.0489624310578622 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11976 0.8305310308335337 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10379 0.7197796901320348 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6705 0.46498919186196097 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3008 0.2086036523670065 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2357 0.16345705074103534 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1589 0.11019654375371453 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 6.934961847307397E-5 0.0 0.0 0.0 0.0 3 6.934961847307397E-5 0.0 0.0 0.0 0.0 4 6.934961847307397E-5 0.0 0.0 0.0 0.0 5 6.934961847307397E-5 0.0 0.0 0.0 0.0 6 6.934961847307397E-5 0.0 0.0 0.0 0.0 7 6.934961847307397E-5 0.0 0.0 0.0 0.0 8 6.934961847307397E-5 0.0 0.0 0.0 0.0 9 6.934961847307397E-5 0.0 0.0 0.0 0.0 10-11 6.934961847307397E-5 0.0 0.0 0.0 0.0 12-13 6.934961847307397E-5 0.0 0.0 0.0 0.0 14-15 6.934961847307397E-5 0.0 0.0 3.4674809236536986E-5 0.0 16-17 6.934961847307397E-5 0.0 0.0 3.120732831288329E-4 0.0 18-19 1.3869923694614795E-4 0.0 0.0 5.201221385480548E-4 0.0 20-21 2.080488554192219E-4 0.0 0.0 6.588213754942028E-4 0.0 22-23 2.080488554192219E-4 0.0 0.0 0.0010749190863326465 0.0 24-25 2.080488554192219E-4 0.0 0.0 0.0016643908433537752 0.0 26-27 2.080488554192219E-4 0.0 0.0 0.002669960311213348 0.0 28-29 2.080488554192219E-4 0.0 0.0 0.006380164899522805 0.0 30-31 2.080488554192219E-4 0.0 0.0 0.01816960003994538 0.0 32-33 2.080488554192219E-4 0.0 0.0 0.04129769780071555 0.0 34-35 2.080488554192219E-4 0.0 0.0 0.07316384748909305 0.0 36-37 2.080488554192219E-4 0.0 0.0 0.12236740179573902 0.0 38-39 2.773984738922959E-4 0.0 0.0 0.20177271494740873 0.0 40-41 2.773984738922959E-4 0.0 0.0 0.29026282811905113 0.0 42-43 2.773984738922959E-4 0.0 0.0 0.3734823702867399 0.0 44-45 2.773984738922959E-4 0.0 0.0 0.47507956134979323 0.0 46-47 2.773984738922959E-4 0.0 0.0 0.594915702071265 0.0 48-49 2.773984738922959E-4 0.0 0.0 0.7258477817484288 0.0 50-51 2.773984738922959E-4 0.0 0.0 0.8884032874493142 0.0 52-53 2.773984738922959E-4 0.0 0.0 1.0362913488431444 0.0 54-55 2.773984738922959E-4 0.0 0.0 1.1945471781986992 0.0 56-57 2.773984738922959E-4 0.0 0.0 1.379537285475624 0.0 58-59 2.773984738922959E-4 0.0 0.0 1.5920938660955959 0.0 60-61 2.773984738922959E-4 0.0 0.0 1.8361698483115796 0.0 62-63 2.773984738922959E-4 0.0 0.0 2.075703430517577 0.0 64-65 2.773984738922959E-4 0.0 0.0 2.3078166035469554 3.4674809236536986E-5 66-67 2.773984738922959E-4 0.0 0.0 2.576581049939354 6.934961847307397E-5 68-69 2.773984738922959E-4 0.0 0.0 2.890908195668562 6.934961847307397E-5 70-71 2.773984738922959E-4 0.0 0.0 3.250069869740612 6.934961847307397E-5 72-73 2.773984738922959E-4 0.0 0.0 3.6167559774169904 6.934961847307397E-5 74-75 2.773984738922959E-4 0.0 0.0 3.9632960209269408 6.934961847307397E-5 76-77 2.773984738922959E-4 0.0 0.0 4.3361195698381865 6.934961847307397E-5 78-79 2.773984738922959E-4 0.0 0.0 4.769693384531845 6.934961847307397E-5 80-81 3.467480923653699E-4 0.0 0.0 5.261590228361358 6.934961847307397E-5 82-83 3.467480923653699E-4 0.0 0.0 5.777586064610265 6.934961847307397E-5 84-85 3.814229016019068E-4 0.0 0.0 6.291674786351162 6.934961847307397E-5 86-87 4.160977108384438E-4 0.0 0.0 6.803336271445502 6.934961847307397E-5 88-89 4.160977108384438E-4 0.0 0.0 7.355359234491171 6.934961847307397E-5 90-91 4.854473293115178E-4 0.0 0.0 7.992335480166355 6.934961847307397E-5 92-93 4.854473293115178E-4 0.0 0.0 8.656912873993823 6.934961847307397E-5 94-95 4.854473293115178E-4 0.0 0.0 9.313376362459941 6.934961847307397E-5 96-97 4.854473293115178E-4 0.0 0.0 9.973723429560552 6.934961847307397E-5 98-99 4.854473293115178E-4 0.0 0.0 10.653176316550494 6.934961847307397E-5 100-101 5.894717570211288E-4 0.0 0.0 11.423061106029326 6.934961847307397E-5 102-103 6.934961847307398E-4 0.0 0.0 12.241906726150146 6.934961847307397E-5 104-105 6.934961847307398E-4 0.0 0.0 13.056383320307162 6.934961847307397E-5 106-107 6.934961847307398E-4 0.0 0.0 13.836219780036881 6.934961847307397E-5 108-109 6.934961847307398E-4 0.0 0.0 14.664566297888513 6.934961847307397E-5 110-111 6.934961847307398E-4 0.0 0.0 15.547941737998528 6.934961847307397E-5 112-113 7.628458032038136E-4 0.0 0.0 16.489986955336764 6.934961847307397E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5240 0.0 76.10729 1 GTATCAA 9640 0.0 54.664658 1 TGGTATC 1985 0.0 44.0569 2 GTGGTAT 2080 0.0 42.9892 1 TATCAAC 12860 0.0 40.94109 2 ATCAACG 13035 0.0 40.392838 3 TCAACGC 13110 0.0 40.11777 4 CAACGCA 13240 0.0 39.7688 5 AACGCAG 13690 0.0 38.72367 6 ACGCAGA 15660 0.0 33.705006 7 CGCAGAG 15780 0.0 33.373272 8 TAAGGTG 1060 0.0 32.554375 5 GTACATG 8375 0.0 32.101337 1 TACATGG 8375 0.0 31.823671 2 GGTAAGG 1085 0.0 30.706509 3 ACATGGG 8305 0.0 30.660294 3 GCAGAGT 17025 0.0 30.478382 9 AGGTAAG 1065 0.0 29.04764 2 GAGTACT 12725 0.0 28.524105 12-13 GTAAGGT 1175 0.0 28.355507 4 >>END_MODULE