FastQCFastQC Report
Thu 26 May 2016
SRR1364108_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364108_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3045682
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN126490.4153092804829919No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29200.044.4303251
GTATCAA41900.041.3318441
ATCAACG43600.038.086733
TCAACGC43600.037.819334
CAACGCA45300.036.7954985
AACGCAG47200.035.4427836
GTACATG95200.034.444551
TACATGG100600.032.18262
ACATGGG101200.031.0534153
TATCAAC55800.030.6103232
ACGCAGA59750.027.8013367
CGCAGAG60300.027.3502838
GTACGGT1401.0541834E-425.5081633
CATGGGG62850.025.0045574
GAGTACA73450.023.7400511
AGTACAT70050.023.1939492
GCAGAGT74050.021.3872999
CATGGGA52000.019.8044724
CAGAGTA68850.019.28398110-11
AGAGTAC64450.018.66054210-11
Produced by FastQC (version 0.11.5)