##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364108_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3045682 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.060912465582422 31.0 31.0 34.0 28.0 34.0 2 31.12469949259312 33.0 31.0 34.0 28.0 34.0 3 31.207725560317854 33.0 31.0 34.0 28.0 34.0 4 34.685003555853825 37.0 35.0 37.0 32.0 37.0 5 34.717918679625775 37.0 35.0 37.0 32.0 37.0 6 34.78774310647008 37.0 35.0 37.0 32.0 37.0 7 34.69612421782708 37.0 35.0 37.0 32.0 37.0 8 34.6567681721204 37.0 35.0 37.0 32.0 37.0 9 36.205368124446345 39.0 37.0 39.0 32.0 39.0 10-11 36.12218856072302 39.0 37.0 39.0 31.0 39.0 12-13 36.03108827513837 39.0 37.0 39.0 31.0 39.0 14-15 37.30540023548092 40.0 38.0 41.0 31.0 41.0 16-17 37.30404503818849 40.0 38.0 41.0 31.0 41.0 18-19 37.29756652204662 40.0 38.0 41.0 31.0 41.0 20-21 37.31086403636361 40.0 38.0 41.0 31.0 41.0 22-23 37.26224290651486 40.0 38.0 41.0 31.0 41.0 24-25 37.259774001356675 40.0 37.0 41.0 31.0 41.0 26-27 37.2184719218881 40.0 37.0 41.0 31.0 41.0 28-29 37.17162034644457 40.0 37.0 41.0 31.0 41.0 30-31 37.12183593034335 40.0 37.0 41.0 31.0 41.0 32-33 37.02903766709722 40.0 37.0 41.0 30.0 41.0 34-35 37.00691749828117 40.0 37.0 41.0 30.0 41.0 36-37 36.952880011767476 40.0 37.0 41.0 30.0 41.0 38-39 36.87786151016422 40.0 37.0 41.0 30.0 41.0 40-41 36.80630183978498 40.0 37.0 41.0 30.0 41.0 42-43 36.70410551725361 40.0 37.0 41.0 30.0 41.0 44-45 36.6153695625479 40.0 36.0 41.0 30.0 41.0 46-47 36.511289425488286 40.0 36.0 41.0 30.0 41.0 48-49 36.40923970394808 40.0 36.0 41.0 30.0 41.0 50-51 36.284860665033314 40.0 36.0 41.0 29.0 41.0 52-53 36.14624737579301 40.0 35.0 41.0 29.0 41.0 54-55 35.97140837421635 39.0 35.0 41.0 28.5 41.0 56-57 35.788282722884404 39.0 35.0 41.0 28.0 41.0 58-59 35.58018975717097 39.0 35.0 41.0 28.0 41.0 60-61 35.3432155753621 38.5 35.0 41.0 28.0 41.0 62-63 35.07944017136392 38.0 34.0 40.0 28.0 41.0 64-65 34.80356205933515 37.5 34.0 40.0 27.0 41.0 66-67 34.499028624787485 37.0 34.0 40.0 26.5 41.0 68-69 34.180710264564716 36.5 34.0 39.5 26.0 41.0 70-71 33.838894211542765 36.0 34.0 39.0 26.0 41.0 72-73 33.48697171930622 35.5 33.0 39.0 26.0 40.5 74-75 33.217316351477265 35.0 33.0 37.5 26.0 40.0 76-77 32.98611805172044 35.0 33.0 37.0 26.0 39.0 78-79 32.688035060784415 35.0 33.0 37.0 26.0 39.0 80-81 32.48491585792607 35.0 33.0 36.0 26.0 39.0 82-83 32.381604514194194 35.0 33.0 36.0 26.0 37.0 84-85 32.17172212988749 35.0 33.0 36.0 26.0 37.0 86-87 31.979231252638982 35.0 33.0 35.0 26.0 37.0 88-89 31.80382521878515 35.0 33.0 35.0 25.0 36.0 90-91 31.664048807459217 35.0 33.0 35.0 25.0 36.0 92-93 31.547863499866367 35.0 33.0 35.0 25.0 36.0 94-95 31.44767362449527 35.0 33.0 35.0 25.0 36.0 96-97 31.364965876279925 35.0 33.0 35.0 24.5 35.0 98-99 31.287595684644685 35.0 33.0 35.0 24.0 35.0 100-101 31.214809523778253 35.0 33.0 35.0 24.0 35.0 102-103 30.113362622887088 34.0 30.5 35.0 19.5 35.0 104-105 30.94377351279615 35.0 32.5 35.0 23.0 35.0 106-107 31.036704751185447 35.0 33.0 35.0 23.0 35.0 108-109 31.011633519192088 35.0 33.0 35.0 23.0 35.0 110-111 30.939764886813528 35.0 33.0 35.0 20.0 35.0 112-113 30.86392390932474 35.0 33.0 35.0 19.5 35.0 114-115 30.764420087192292 35.0 33.0 35.0 19.0 35.0 116-117 30.661828779235652 35.0 33.0 35.0 17.5 35.0 118-119 30.52327196338948 35.0 32.5 35.0 11.0 35.0 120-121 30.381568397488643 35.0 32.0 35.0 2.0 35.0 122-123 30.21278830160207 35.0 32.0 35.0 2.0 35.0 124-125 29.38264943615256 34.5 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.395867040540022 1101 2 0.4923786968959689 1101 3 0.46401243116109825 1101 4 0.42220990937881453 1101 5 0.43769243256806334 1101 6 0.4620680124822627 1101 7 0.4339944311085375 1101 8 0.42341794344383743 1101 9 0.4676045183835811 1101 10-11 0.505150553189587 1101 12-13 0.5947915530059404 1101 14-15 0.698114028433082 1101 16-17 0.6933160840211272 1101 18-19 0.7371431960749675 1101 20-21 0.683864429540975 1101 22-23 0.6993936521244422 1101 24-25 0.6576325763005784 1101 26-27 0.6668710822999131 1101 28-29 0.683479332933878 1101 30-31 0.7298477627323834 1101 32-33 0.692426687927842 1101 34-35 0.7530891176439809 1101 36-37 0.7922337295582977 1101 38-39 0.7423295423591867 1101 40-41 0.8264024797692571 1101 42-43 0.8342656446487453 1101 44-45 0.7946316573555166 1101 46-47 0.8254207362769961 1101 48-49 0.7023620216154995 1101 50-51 0.7085228060997224 1101 52-53 0.7445606969805212 1101 54-55 0.7624335361607635 1101 56-57 0.8119251471994673 1101 58-59 0.7967187153305986 1101 60-61 0.8955864785575329 1101 62-63 0.8383635712696886 1101 64-65 0.8210597425990187 1101 66-67 0.8419707818403026 1101 68-69 0.8346374232715235 1101 70-71 0.8793856724105069 1101 72-73 0.8362075768194543 1101 74-75 0.6997482951846123 1101 76-77 0.7464169641276754 1101 78-79 0.7235514167398804 1101 80-81 0.6829639624945401 1101 82-83 0.7033164939401999 1101 84-85 0.7166577548459898 1101 86-87 0.6617217056975235 1101 88-89 0.736652588049477 1101 90-91 0.7305486745824759 1101 92-93 0.7166227952161393 1101 94-95 0.7333534645125255 1101 96-97 0.6826188749054758 1101 98-99 0.7029041926957404 1101 100-101 0.7477198262255733 1101 102-103 0.7935760955406614 1101 104-105 0.7679285957811253 1101 106-107 0.7739781092967526 1101 108-109 0.8100726622434209 1101 110-111 0.7999358496448004 1101 112-113 0.8325985291191209 1101 114-115 0.8776598221524701 1101 116-117 0.9132405383649385 1101 118-119 0.9445357139759238 1101 120-121 0.9754585460373697 1101 122-123 0.9361950260033041 1101 124-125 1.0258848731188444 1102 1 0.43049717009466804 1102 2 0.43006883909535176 1102 3 0.4287041630044186 1102 4 0.4694871507406617 1102 5 0.495972188335287 1102 6 0.47458953757096367 1102 7 0.4143757972645048 1102 8 0.3694192158316838 1102 9 0.4955762270940198 1102 10-11 0.5022063137915254 1102 12-13 0.5496140681572186 1102 14-15 0.5547075240599995 1102 16-17 0.5773561390121813 1102 18-19 0.5506077443639299 1102 20-21 0.6063437646149481 1102 22-23 0.6946818748799615 1102 24-25 0.5578539531880509 1102 26-27 0.6044034867894581 1102 28-29 0.605252607506408 1102 30-31 0.6346768465785075 1102 32-33 0.6067723415540343 1102 34-35 0.6251799599237771 1102 36-37 0.5111751547058887 1102 38-39 0.5362081518891557 1102 40-41 0.5297252006374151 1102 42-43 0.5957042996174735 1102 44-45 0.5779290788559024 1102 46-47 0.535130375233237 1102 48-49 0.5412938895099515 1102 50-51 0.6093522571596282 1102 52-53 0.5743493670305284 1102 54-55 0.5900007458296415 1102 56-57 0.5934188980854742 1102 58-59 0.588254259537571 1102 60-61 0.606553320201435 1102 62-63 0.5665687357477296 1102 64-65 0.5790651415913359 1102 66-67 0.5556829719035079 1102 68-69 0.5377140901297821 1102 70-71 0.5451785163516689 1102 72-73 0.5921324356988578 1102 74-75 0.552745257262167 1102 76-77 0.5705878172656114 1102 78-79 0.5436442381357764 1102 80-81 0.5710959978115682 1102 82-83 0.6298189814871265 1102 84-85 0.6389458409845332 1102 86-87 0.6146001987914431 1102 88-89 0.6163368514648262 1102 90-91 0.6270843360169103 1102 92-93 0.6013244434144482 1102 94-95 0.6492575014206778 1102 96-97 0.6440025665179157 1102 98-99 0.685806567546031 1102 100-101 0.6923215164971701 1102 102-103 0.7364171975262757 1102 104-105 0.75134533702985 1102 106-107 0.7351447075230233 1102 108-109 0.6829233313747984 1102 110-111 0.7122562134001278 1102 112-113 0.7391421016759416 1102 114-115 0.7734194081189294 1102 116-117 0.7378774077566312 1102 118-119 0.7127471731234394 1102 120-121 0.7603300306108771 1102 122-123 0.7860757903421778 1102 124-125 0.8071195612092161 1103 1 0.365743720401035 1103 2 0.4312133666297484 1103 3 0.4387858008130081 1103 4 0.45410820643841987 1103 5 0.39098949802536254 1103 6 0.35876219109619 1103 7 0.3337297836487494 1103 8 0.4056967431366587 1103 9 0.386679845502357 1103 10-11 0.49981962588407924 1103 12-13 0.43285679306074343 1103 14-15 0.5542457819550535 1103 16-17 0.5870217900395502 1103 18-19 0.5374302111300935 1103 20-21 0.5638625242976758 1103 22-23 0.6260585559047769 1103 24-25 0.6178105049637352 1103 26-27 0.6540493019173326 1103 28-29 0.6904992867010051 1103 30-31 0.5614535064656536 1103 32-33 0.36237414650489086 1103 34-35 0.6518263138044702 1103 36-37 0.7019967656541652 1103 38-39 0.7390995016840947 1103 40-41 0.7433967624792786 1103 42-43 0.8182401498802747 1103 44-45 0.757340858725108 1103 46-47 0.6675101933977103 1103 48-49 0.6755876979768729 1103 50-51 0.6603736319846547 1103 52-53 0.6462119695933453 1103 54-55 0.7109414108413858 1103 56-57 0.7781550509778512 1103 58-59 0.8150640437013266 1103 60-61 0.7895137154243201 1103 62-63 0.764846531140229 1103 64-65 0.820593962289756 1103 66-67 0.7849767545927762 1103 68-69 0.7843285183944531 1103 70-71 0.7510558843507837 1103 72-73 0.7904492197827153 1103 74-75 0.6812842059981605 1103 76-77 0.6744888130561151 1103 78-79 0.7378910539519126 1103 80-81 0.6261464188894337 1103 82-83 0.6489479290115696 1103 84-85 0.6229643441796711 1103 86-87 0.6070322038026106 1103 88-89 0.5852751088443249 1103 90-91 0.5347529927179373 1103 92-93 0.5242969670203266 1103 94-95 0.567365138564206 1103 96-97 0.49709897749610477 1103 98-99 0.5033539919724106 1103 100-101 0.5046673339450258 1103 102-103 0.5939409194060872 1103 104-105 0.6418029031559591 1103 106-107 0.6302731833165254 1103 108-109 0.6125790322303608 1103 110-111 0.5103112254769577 1103 112-113 0.47568069210263886 1103 114-115 0.5694721414517439 1103 116-117 0.6095433913156469 1103 118-119 0.6855968662405516 1103 120-121 0.6771529742635174 1103 122-123 0.6315713398313925 1103 124-125 0.7167344302716536 1104 1 0.2866542149027822 1104 2 0.32017705374314076 1104 3 0.3137845622709783 1104 4 0.2619762413880409 1104 5 0.24808200166958727 1104 6 0.16949592087954102 1104 7 0.28668952025020644 1104 8 0.30511212215674277 1104 9 0.3334088414323588 1104 10-11 0.2839132637413826 1104 12-13 0.3241055173624616 1104 14-15 0.370001945377318 1104 16-17 0.45046575577535464 1104 18-19 0.3598970138323878 1104 20-21 0.32587951476294563 1104 22-23 0.36649001732025965 1104 24-25 0.34860457942897227 1104 26-27 0.4416919455313817 1104 28-29 0.4579488867830719 1104 30-31 0.3743734814966473 1104 32-33 -0.29032038182041475 1104 34-35 0.38297089566394504 1104 36-37 0.3960118974321247 1104 38-39 0.4899985374864144 1104 40-41 0.5172353451908691 1104 42-43 0.5020636835776529 1104 44-45 0.5192407705409181 1104 46-47 0.47964143572238527 1104 48-49 0.5535301095610023 1104 50-51 0.5504708764763535 1104 52-53 0.5175952811574831 1104 54-55 0.44891247208830976 1104 56-57 0.45330485627904693 1104 58-59 0.5234182865383303 1104 60-61 0.5111730324368722 1104 62-63 0.5567781729457195 1104 64-65 0.5508185259878289 1104 66-67 0.4230034482334375 1104 68-69 0.3887608523263708 1104 70-71 0.48065105013181864 1104 72-73 0.4275212160851538 1104 74-75 0.5004302589541467 1104 76-77 0.5051699907726217 1104 78-79 0.4427486740744584 1104 80-81 0.4450321594530493 1104 82-83 0.37838521711159956 1104 84-85 0.42847434081666336 1104 86-87 0.42964216739239447 1104 88-89 0.41965938151706794 1104 90-91 0.3837733351150625 1104 92-93 0.35297513085409093 1104 94-95 0.3222312973412116 1104 96-97 0.3943957210922555 1104 98-99 0.3765145384929198 1104 100-101 0.3657418230682943 1104 102-103 0.3984111235819263 1104 104-105 0.43248747884894456 1104 106-107 0.41301477041050916 1104 108-109 0.4081794540923447 1104 110-111 0.3636240631253713 1104 112-113 0.3871104387931119 1104 114-115 0.4312765689626481 1104 116-117 0.4560166356021824 1104 118-119 0.5034012139019204 1104 120-121 0.4885893945098623 1104 122-123 0.5286581869813176 1104 124-125 0.6344355709542953 1105 1 0.25451209000519626 1105 2 0.29458220030404547 1105 3 0.2556565543208471 1105 4 0.28289200122205216 1105 5 0.3004319680803462 1105 6 0.23720175186720382 1105 7 0.24080163292823897 1105 8 0.25280035537030443 1105 9 0.27038224819930434 1105 10-11 0.2889044891195027 1105 12-13 0.3286669369047033 1105 14-15 0.4325233425725443 1105 16-17 0.4252238044001615 1105 18-19 0.4076124768913587 1105 20-21 0.38101413768676196 1105 22-23 0.27462765686120605 1105 24-25 0.3938133543103177 1105 26-27 0.43352806116678266 1105 28-29 0.49144844373950747 1105 30-31 0.45205001505420483 1105 32-33 0.07485944975815784 1105 34-35 0.44601288887132284 1105 36-37 0.43233976418299136 1105 38-39 0.44232725625707303 1105 40-41 0.5099186996637286 1105 42-43 0.49445532746027965 1105 44-45 0.5259336330203865 1105 46-47 0.49312474991026534 1105 48-49 0.47770544093553013 1105 50-51 0.5570521672229205 1105 52-53 0.5790513172578997 1105 54-55 0.5012387979768462 1105 56-57 0.5679334720837019 1105 58-59 0.6102814453455068 1105 60-61 0.5723315919660479 1105 62-63 0.6269717604732818 1105 64-65 0.604258527087211 1105 66-67 0.5533702612070641 1105 68-69 0.6373774613404279 1105 70-71 0.653385114485836 1105 72-73 0.5484079153747814 1105 74-75 0.5273391554448281 1105 76-77 0.5222471688611421 1105 78-79 0.46036470711506894 1105 80-81 0.48711135203119227 1105 82-83 0.5118573231933468 1105 84-85 0.45411436110715186 1105 86-87 0.4961454309616826 1105 88-89 0.4872306969723077 1105 90-91 0.44671764299385686 1105 92-93 0.5307754263894466 1105 94-95 0.4984817452258028 1105 96-97 0.5229291442980148 1105 98-99 0.48577843749830407 1105 100-101 0.4567228773519787 1105 102-103 0.4017180578516175 1105 104-105 0.4948280644890666 1105 106-107 0.5730864040758235 1105 108-109 0.5163264585474785 1105 110-111 0.5342067919076037 1105 112-113 0.5369535584617715 1105 114-115 0.5495688891131714 1105 116-117 0.62509511603362 1105 118-119 0.6950853430823862 1105 120-121 0.7305502114974836 1105 122-123 0.7108852664018954 1105 124-125 0.6906184732541156 1106 1 0.2099827722524168 1106 2 0.278938971308353 1106 3 0.2567454361165922 1106 4 0.26788083748121494 1106 5 0.2134571778301293 1106 6 0.19312770640763688 1106 7 0.27916926205273285 1106 8 0.24782900093078553 1106 9 0.31163078835879787 1106 10-11 0.28169193994515496 1106 12-13 0.2536703611569422 1106 14-15 0.3555189640688283 1106 16-17 0.3467010326665019 1106 18-19 0.345975094230198 1106 20-21 0.366606096255623 1106 22-23 0.311575303448798 1106 24-25 0.3613786077828962 1106 26-27 0.3883518674200346 1106 28-29 0.34542241773783644 1106 30-31 0.3432196979719535 1106 32-33 0.3034778314811106 1106 34-35 0.34882411689966375 1106 36-37 0.33300521326496124 1106 38-39 0.33835277920836404 1106 40-41 0.33333556852934976 1106 42-43 0.27521167564021454 1106 44-45 0.3363135886106363 1106 46-47 0.28182634599319556 1106 48-49 0.3287082356026687 1106 50-51 0.31340048754238126 1106 52-53 0.2856311086057062 1106 54-55 0.3136982936470787 1106 56-57 0.38307541956440616 1106 58-59 0.33555451864032193 1106 60-61 0.3047427395811937 1106 62-63 0.34536467220797107 1106 64-65 0.39780700188759255 1106 66-67 0.4181829821017544 1106 68-69 0.42021522141572376 1106 70-71 0.3919341552763882 1106 72-73 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2216 72-73 -1.9559504309762161 2216 74-75 -2.0744952712423697 2216 76-77 -2.0781177637761807 2216 78-79 -2.0324724854375944 2216 80-81 -1.8849737086244005 2216 82-83 -1.7244960957334712 2216 84-85 -1.7035847918939595 2216 86-87 -1.8258146005038043 2216 88-89 -1.7817617961653518 2216 90-91 -1.770319945428799 2216 92-93 -1.9104535529296598 2216 94-95 -1.8558317161996278 2216 96-97 -1.7819840260558415 2216 98-99 -1.7696668030750402 2216 100-101 -1.8304745692269258 2216 102-103 -2.0528966726706948 2216 104-105 -1.857523326777315 2216 106-107 -1.774913191755001 2216 108-109 -1.8190827240025236 2216 110-111 -1.8537765901032905 2216 112-113 -1.8191224034387545 2216 114-115 -1.8745643507469119 2216 116-117 -1.8997906151112147 2216 118-119 -1.9858478788326543 2216 120-121 -2.017186852847882 2216 122-123 -2.075458735837504 2216 124-125 -2.3488825327677354 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 57123.0 3 9630.0 4 5551.0 5 4694.0 6 4904.0 7 5781.0 8 6400.0 9 7556.0 10 8221.0 11 8940.0 12 9502.0 13 9704.0 14 9546.0 15 8765.0 16 7832.0 17 7306.0 18 7264.0 19 7136.0 20 7623.0 21 8236.0 22 10192.0 23 13046.0 24 17051.0 25 22114.0 26 27244.0 27 32889.0 28 39754.0 29 49264.0 30 59664.0 31 72489.0 32 92727.0 33 121911.0 34 167999.0 35 249897.0 36 468756.0 37 934846.0 38 461425.0 39 2700.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.597221768607845 18.128245198532994 12.451599345343723 24.822933687515444 2 15.929210254082527 19.356408273779994 39.72338704569685 24.990994426440626 3 19.31214609968287 23.220379908258877 29.19539486748468 28.272079124573573 4 12.77675194594691 15.364058151140982 36.84481176480678 35.01437813810533 5 13.972308200824756 37.29634671850563 34.70533301181386 14.026012068855747 6 33.79677259328279 35.51569953715485 16.993278959012642 13.69424891054972 7 29.472973044334662 31.50210702526582 20.737663472683924 18.287256457715596 8 26.22242672239223 35.77002840960665 19.526348119509812 18.481196748491307 9 26.136346756216373 15.801885468936042 19.716054016351205 38.34571375849638 10-11 25.305934661963864 25.95780288975985 27.899887514432127 20.836374933844155 12-13 25.42090791197416 23.29491826513909 28.05387409950496 23.23029972338179 14-15 22.39859514944281 25.348877053788456 25.14341155261472 27.10911624415402 16-17 22.996070397794444 28.443675179071786 25.82170674033481 22.738547682798956 18-19 22.90523965950937 27.654142757608323 27.022948569368555 22.417669013513752 20-21 23.455439842421345 27.0119859409945 27.01292681747211 22.519647399112046 22-23 23.19003247537933 26.913904586569537 26.82898523354143 23.067077704509707 24-25 22.974520864115952 27.066441925691866 26.95647049482357 23.002566715368612 26-27 22.99617659003338 27.29460727504449 26.80608245754793 22.903133677374196 28-29 22.79938316790453 27.332039436189188 26.98488569905849 22.883691696847787 30-31 23.02501342371722 27.090092991325793 26.936436202391196 22.948457382565792 32-33 22.808150698826342 27.210955544929057 26.95685488283787 23.02403887340673 34-35 23.242901877036353 27.219709335597102 26.67190133513757 22.865487452228976 36-37 23.257000990001497 27.105683360864536 26.640196238390253 22.997119410743718 38-39 22.912104603309523 27.228175237326248 26.87589241627985 22.98382774308438 40-41 23.166482318671743 27.035514378731317 26.879158034029643 22.918845268567296 42-43 23.0380819128356 27.074775559186715 26.77470745710385 23.112435070873833 44-45 22.981964257084552 27.16511717360854 26.84367241400924 23.009246155297667 46-47 22.973105157059372 27.301596779751346 26.62526274707875 23.100035316110528 48-49 23.03806168092752 27.196909981585666 26.69514328107745 23.06988505640937 50-51 22.959654776659487 27.181538165144936 26.753621285561735 23.10518577263384 52-53 23.075146754962386 27.156344157355427 26.713188623403898 23.055320464278285 54-55 23.010573856686904 26.954032618777767 26.767614628179974 23.267778896355356 56-57 22.87940128811311 27.28615176991464 26.821227927289566 23.013219014682683 58-59 23.027300491114275 27.11672528030392 26.722854172690074 23.13312005589173 60-61 23.06264320547389 27.119803846124036 26.907827172299122 22.909725776102956 62-63 22.91382885193659 27.16792000620209 26.87515154714168 23.043099594719642 64-65 22.772906647383017 27.09689902277551 26.81767317511855 23.31252115472292 66-67 22.77696524879718 27.256241580757262 26.731712241789268 23.23508092865629 68-69 22.64091489509128 27.06847855137211 26.95634385115137 23.33426270238523 70-71 22.69904694628034 26.994478257075233 26.958019675426236 23.348455121218183 72-73 22.85417538024186 26.97251006237476 26.856538048109655 23.31677650927372 74-75 22.654520492668034 27.151991212891236 26.741159563792245 23.45232873064849 76-77 22.82903022202543 27.033451462270392 26.768246994508836 23.36927132119534 78-79 22.78845950783234 26.98065541400265 26.89783112556558 23.33305395259943 80-81 22.657759274052122 27.159513135566517 26.917031181483342 23.26569640889802 82-83 22.77979098732874 27.105523434941524 26.94377742270499 23.17090815502474 84-85 22.884398601210552 26.919075011956554 26.783696583385673 23.412829803447217 86-87 22.71503128114629 26.8680682230204 27.01705905427773 23.399841441555573 88-89 22.81359017364584 26.836044812276704 26.969767005657214 23.380598008420243 90-91 22.795499645951168 26.916420228996113 27.087317035596055 23.200763089456668 92-93 22.73385844538726 26.65349433074934 27.081488035210683 23.531159188652722 94-95 22.64490865236174 26.725847568329304 27.0949722513241 23.534271527984863 96-97 22.807341010949113 26.707805546723378 27.010311216540167 23.474542225787342 98-99 22.743987742541947 26.546599013692706 26.93930520628739 23.770108037477964 100-101 22.578355844435023 26.58033802590288 27.164145199078675 23.677160930583423 102-103 22.683294294507146 26.369010040203673 27.25337544361678 23.6943202216724 104-105 22.568753530751557 26.462812933591128 27.249250495726997 23.719183039930318 106-107 22.630846612907135 26.473852601470032 27.1746969411992 23.720603844423636 108-109 22.611812961714293 26.459936264674706 26.996806639288724 23.93144413432228 110-111 22.46227136911397 26.610392522871333 27.181813084975165 23.74552302303953 112-113 22.48616555232009 26.610338944174277 27.24816061497354 23.65533488853209 114-115 22.4280302230852 26.619798152212915 27.119685369662943 23.83248625503894 116-117 22.41868227369937 26.55397766477844 27.274935125040212 23.752404936481977 118-119 22.4414738171754 26.593427873418797 27.020311443564193 23.94478686584161 120-121 22.463084232999154 26.762001335314313 27.097033939678628 23.677880492007905 122-123 22.48677296658011 26.770519770600064 27.127236237974316 23.615471024845505 124-125 22.529593236152103 26.74911620573382 26.936040816050582 23.785249742063495 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 12540.0 1 6602.5 2 665.0 3 580.5 4 459.0 5 404.5 6 360.0 7 343.0 8 351.0 9 334.5 10 339.5 11 346.0 12 332.0 13 359.5 14 418.5 15 473.0 16 547.5 17 666.0 18 844.0 19 1118.0 20 1520.0 21 2173.5 22 3117.5 23 4505.0 24 6461.5 25 9162.5 26 13034.0 27 17649.0 28 21574.0 29 25774.0 30 31604.0 31 37802.5 32 44010.5 33 50128.5 34 57142.0 35 63040.5 36 69411.0 37 76323.5 38 80415.0 39 84380.5 40 88138.5 41 92495.5 42 97045.5 43 102621.5 44 108547.0 45 112357.0 46 113693.5 47 114738.0 48 117536.5 49 118328.0 50 116818.5 51 113423.5 52 111010.0 53 109755.5 54 107193.0 55 102283.5 56 93818.5 57 85112.5 58 77979.0 59 69303.0 60 58096.5 61 47210.5 62 38021.5 63 30327.0 64 24006.0 65 18804.0 66 14491.0 67 11708.0 68 8639.5 69 5576.0 70 4039.0 71 3001.0 72 2275.5 73 1789.0 74 1438.0 75 1157.0 76 746.0 77 390.0 78 234.0 79 161.5 80 95.0 81 45.0 82 27.5 83 19.5 84 17.0 85 15.5 86 16.5 87 14.5 88 9.5 89 11.5 90 10.5 91 7.5 92 6.5 93 7.0 94 7.0 95 3.5 96 3.5 97 5.5 98 3.0 99 1.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4751973449624748 2 0.46708750289754475 3 0.4632131653928414 4 0.4595358281002416 5 0.46764567016517156 6 0.5234623969278474 7 0.5193582258423565 8 0.5277307348567578 9 0.535282409654061 10-11 0.5532094289554852 12-13 0.562747522558166 14-15 0.5405357486434894 16-17 0.5332303241113157 18-19 0.5444593362012186 20-21 0.544754836519374 22-23 0.5557375983441476 24-25 0.5487605074988131 26-27 0.5572479333036082 28-29 0.5362509940302369 30-31 0.5426863342922866 32-33 0.5439668356709597 34-35 0.5423580006054473 36-37 0.48851127596380706 38-39 0.4558913241763257 40-41 0.45393773873963206 42-43 0.4663487521021564 44-45 0.4717498412506624 46-47 0.47567342880839164 48-49 0.4889216930723562 50-51 0.47178267461934636 52-53 0.4712737574047454 54-55 0.4614401634839093 56-57 0.4456308964625985 58-59 0.4631639153398155 60-61 0.45902691088564074 62-63 0.47434367737669264 64-65 0.47424517727064086 66-67 0.4819610189113637 68-69 0.49092452856207575 70-71 0.4874277747972376 72-73 0.49937912099818693 74-75 0.48851127596380706 76-77 0.48636069031500995 78-79 0.4980822029351718 80-81 0.49056336150655255 82-83 0.4926482804179819 84-85 0.4898246107111642 86-87 0.4801223502650638 88-89 0.4849981055146269 90-91 0.4701902562381759 92-93 0.47046933987198924 94-95 0.46577416815018774 96-97 0.4543481558481811 98-99 0.41872395082612035 100-101 0.41567044753851523 102-103 0.40637860420096383 104-105 0.4107618589202681 106-107 0.40987535796580205 108-109 0.42115362010873103 110-111 0.44331614397038166 112-113 0.4428072267557808 114-115 0.4366181367588606 116-117 0.43069171371141174 118-119 0.47314525941972935 120-121 0.46577416815018774 122-123 0.4321363819335045 124-125 0.4413953919023719 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 3045682.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.9305574948752 #Duplication Level Percentage of deduplicated Percentage of total 1 85.00056860761386 61.991388559346674 2 9.631187276965516 14.04815714893288 3 2.637525321042346 5.770685762114039 4 1.0362368685962398 3.0229333009387 5 0.5391494874144012 1.9660236345104238 6 0.2881105336786048 1.2607237104796 7 0.19114809993194634 0.9758376244486074 8 0.12144349569156539 0.7085553475929871 9 0.08471493421278478 0.5560476642252064 >10 0.4267913181073237 5.70821567374339 >50 0.027492624533112676 1.3813311161653918 >100 0.015531236648684507 1.6857977631505772 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 1.0019556371752516E-4 0.9243026943514943 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 12649 0.4153092804829919 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.283336868392695E-5 2 3.283336868392695E-5 0.0 0.0 0.0 3.283336868392695E-5 3 3.283336868392695E-5 0.0 0.0 0.0 3.283336868392695E-5 4 3.283336868392695E-5 0.0 0.0 0.0 3.283336868392695E-5 5 9.850010605178085E-5 0.0 0.0 0.0 3.283336868392695E-5 6 9.850010605178085E-5 0.0 0.0 0.0 3.283336868392695E-5 7 1.313334747357078E-4 0.0 0.0 0.0 3.283336868392695E-5 8 1.313334747357078E-4 0.0 0.0 0.0 3.283336868392695E-5 9 1.313334747357078E-4 0.0 0.0 0.0 3.283336868392695E-5 10-11 1.313334747357078E-4 0.0 0.0 0.0 3.283336868392695E-5 12-13 1.4775015907767127E-4 0.0 0.0 0.0 3.283336868392695E-5 14-15 1.970002121035617E-4 0.0 0.0 4.9250053025890425E-5 3.283336868392695E-5 16-17 1.970002121035617E-4 0.0 0.0 9.850010605178085E-5 3.283336868392695E-5 18-19 2.4625026512945213E-4 0.0 0.0 1.1491679039374433E-4 3.283336868392695E-5 20-21 2.9550031815534254E-4 0.0 0.0 1.313334747357078E-4 3.283336868392695E-5 22-23 2.9550031815534254E-4 0.0 0.0 1.6416684341963474E-4 3.283336868392695E-5 24-25 4.1041710854908687E-4 0.0 0.0 1.9700021210356167E-4 3.283336868392695E-5 26-27 4.432504772330138E-4 0.0 0.0 4.432504772330138E-4 3.283336868392695E-5 28-29 6.074173206526486E-4 0.0 0.0 0.0014118348534088588 3.283336868392695E-5 30-31 7.223341110463929E-4 0.0 0.0 0.005450339201531874 3.283336868392695E-5 32-33 7.223341110463929E-4 0.0 0.0 0.014512348958295711 3.283336868392695E-5 34-35 7.715841640722834E-4 0.0 0.0 0.028466530648964664 3.283336868392695E-5 36-37 8.865009544660276E-4 0.0 0.0 0.054372058540583024 3.283336868392695E-5 38-39 9.193343231499546E-4 0.0 0.0 0.09355868406484985 3.283336868392695E-5 40-41 9.35751007491918E-4 0.0 0.0 0.13445264476068086 3.283336868392695E-5 42-43 0.0010342511135436989 0.0 0.0 0.17562568909032525 3.283336868392695E-5 44-45 0.0011820012726213702 0.0 0.0 0.22993208089354045 3.283336868392695E-5 46-47 0.0012969180630151146 0.0 0.0 0.2976837371728237 3.283336868392695E-5 48-49 0.001379001484724932 0.0 0.0 0.37339748535795925 3.283336868392695E-5 50-51 0.001379001484724932 0.0 0.0 0.4540854888987097 3.283336868392695E-5 52-53 0.001379001484724932 0.0 0.0 0.5326557401593469 3.283336868392695E-5 54-55 0.0014446682220927859 0.0 0.0 0.6238832550476379 3.283336868392695E-5 56-57 0.0015431683281445666 0.0 0.0 0.7353853750982539 3.283336868392695E-5 58-59 0.0015431683281445666 0.0 0.0 0.8637966800210921 3.283336868392695E-5 60-61 0.0015431683281445666 0.0 0.0 1.00415933114488 3.283336868392695E-5 62-63 0.00155958501248653 0.0 0.0 1.1428967305188134 3.283336868392695E-5 64-65 0.0015760016968284936 0.0 0.0 1.2928303086139656 3.283336868392695E-5 66-67 0.0016088350655124204 0.0 0.0 1.4693096652900728 3.283336868392695E-5 68-69 0.0016088350655124204 0.0 0.0 1.6749122199888236 3.283336868392695E-5 70-71 0.0016745018028802745 0.0 0.0 1.8995252951555677 3.283336868392695E-5 72-73 0.0017401685402481282 0.0 0.0 2.130508043846994 3.283336868392695E-5 74-75 0.0017894185932740188 0.0 0.0 2.3697648014467694 3.283336868392695E-5 76-77 0.0018058352776159823 0.0 0.0 2.6350255870442156 3.283336868392695E-5 78-79 0.0018058352776159823 0.0 0.0 2.9400804154865807 3.283336868392695E-5 80-81 0.002134168964455252 0.0 0.0 3.2814489496933694 3.283336868392695E-5 82-83 0.002134168964455252 0.0 0.0 3.637510416386215 3.283336868392695E-5 84-85 0.002183419017481142 0.0 0.0 3.989319961834492 3.283336868392695E-5 86-87 0.0022326690705070326 0.0 0.0 4.366279867694658 3.283336868392695E-5 88-89 0.0022490857548489963 1.6416684341963475E-5 0.0 4.786875320535762 3.283336868392695E-5 90-91 0.0023640025452427403 3.283336868392695E-5 0.0 5.245672397840615 3.283336868392695E-5 92-93 0.0024296692826105944 3.283336868392695E-5 0.0 5.726566332269751 3.283336868392695E-5 94-95 0.0024296692826105944 3.283336868392695E-5 0.0 6.218262444995899 3.283336868392695E-5 96-97 0.0024296692826105944 3.283336868392695E-5 0.0 6.7113539758911145 3.283336868392695E-5 98-99 0.0024789193356364848 3.283336868392695E-5 0.0 7.247818386817796 3.283336868392695E-5 100-101 0.002561002757346302 3.283336868392695E-5 0.0 7.843858945221465 3.283336868392695E-5 102-103 0.002593836126030229 3.283336868392695E-5 0.0 8.463523112393219 3.283336868392695E-5 104-105 0.002905753128527535 3.283336868392695E-5 0.0 9.079772609221841 3.283336868392695E-5 106-107 0.003037086603263243 6.56667373678539E-5 0.0 9.691425434434718 3.283336868392695E-5 108-109 0.0030863366562891336 6.56667373678539E-5 0.0 10.342297718540543 3.283336868392695E-5 110-111 0.0031355867093150235 6.56667373678539E-5 0.0 11.044816891586187 3.283336868392695E-5 112-113 0.0031520033936569872 6.56667373678539E-5 0.0 11.778478514828535 3.283336868392695E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2920 0.0 44.430325 1 GTATCAA 4190 0.0 41.331844 1 ATCAACG 4360 0.0 38.08673 3 TCAACGC 4360 0.0 37.81933 4 CAACGCA 4530 0.0 36.795498 5 AACGCAG 4720 0.0 35.442783 6 GTACATG 9520 0.0 34.44455 1 TACATGG 10060 0.0 32.1826 2 ACATGGG 10120 0.0 31.053415 3 TATCAAC 5580 0.0 30.610323 2 ACGCAGA 5975 0.0 27.801336 7 CGCAGAG 6030 0.0 27.350283 8 GTACGGT 140 1.0541834E-4 25.508163 3 CATGGGG 6285 0.0 25.004557 4 GAGTACA 7345 0.0 23.740051 1 AGTACAT 7005 0.0 23.193949 2 GCAGAGT 7405 0.0 21.387299 9 CATGGGA 5200 0.0 19.804472 4 CAGAGTA 6885 0.0 19.283981 10-11 AGAGTAC 6445 0.0 18.660542 10-11 >>END_MODULE