FastQCFastQC Report
Thu 26 May 2016
SRR1364108_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364108_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3045682
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52760.1732288531763986No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52410.17207968527246115No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45550.064.125121
GTATCAA75500.048.6355971
ATCAACG89500.040.2835733
TCAACGC90200.039.9716074
CAACGCA92250.039.0833475
AACGCAG96450.037.628176
TATCAAC97750.036.8842852
GTACATG88950.032.569481
ACGCAGA112050.032.2338567
CGCAGAG114800.031.3580388
TACATGG92200.030.586722
ACATGGG93700.029.0171663
GCAGAGT133050.026.5201059
GTGGTAT25650.026.028621
CAGAGTA123500.025.27029810-11
GAGTACT87750.025.12287712-13
CATGGGG58850.023.8589344
TGGTATC26200.023.8436932
AGAGTAC118350.023.83098210-11
GTACTTT92600.023.45094114-15
Produced by FastQC (version 0.11.5)