FastQCFastQC Report
Thu 26 May 2016
SRR1364107_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364107_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2019048
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN81030.40132775446646146No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17550.057.3067171
GTATCAA30050.046.5392461
ATCAACG33900.040.0329443
TCAACGC34150.039.5754244
CAACGCA34950.038.5011145
AACGCAG38500.034.9510156
TATCAAC38950.034.8373372
GTACATG73300.034.261351
TACATGG73600.032.9913372
ACATGGG73950.031.8742073
ACGCAGA46350.028.3914157
CGCAGAG46600.028.111328
GTATAGG4050.026.4492551
CATGGGG45850.025.580994
GTGGTAT12650.023.5220641
TAAGGTG12950.023.4483595
CATGGGA30250.023.4213544
GAGTACA49600.023.156431
CTTAGCG1552.1015995E-423.0410333
GTAAGGT12300.022.7500934
Produced by FastQC (version 0.11.5)