FastQCFastQC Report
Thu 26 May 2016
SRR1364107_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364107_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2019048
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42460.21029713013261697No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38640.1913773223816373No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20680.10242450897650773No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33400.072.096541
GTATCAA59800.053.5244981
ATCAACG76100.041.7483673
TGGTATC13500.041.4263762
TATCAAC77250.041.357922
TCAACGC77100.041.2068864
CAACGCA79200.040.1142775
GTGGTAT14800.039.4678881
AACGCAG83900.037.9380236
ACGCAGA96250.032.8903927
CGCAGAG97450.032.1190078
GTACATG73400.027.447331
TACATGG72050.027.3323442
GCAGAGT113500.027.2624999
GAGTACT69500.026.49927512-13
TAAGGTG13700.026.4906225
ACATGGG71700.026.2210963
CAGAGTA103050.025.7540210-11
GTACTTT71800.025.60453814-15
AGAGTAC97500.024.53463710-11
Produced by FastQC (version 0.11.5)