FastQCFastQC Report
Thu 26 May 2016
SRR1364106_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364106_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2203418
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN88270.4006048784207082No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25000.048.7970281
GTATCAA31250.041.5131761
TCAACGC35450.035.765294
ATCAACG35800.035.5737843
CAACGCA36150.035.5683445
GTACATG83100.035.447341
TACATGG81150.035.3466222
ACATGGG81150.033.660733
TATCAAC39450.032.734232
AACGCAG39500.032.7032366
CATGGGG54850.029.084164
GAGTACA65700.025.1801851
AGTACAT59450.025.0251982
ACGCAGA50700.025.0103387
CGCAGAG52000.024.1561138
GGACCGA4050.023.5176016
CATGGGA33600.022.498984
ATGGGGG32000.020.0905575
CAGAGTA56300.019.08673310-11
ATGGGGC19050.019.0613235
Produced by FastQC (version 0.11.5)