FastQCFastQC Report
Thu 26 May 2016
SRR1364106_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364106_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2203418
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43000.19515135121887903No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41360.18770836945146133No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23290.10569941790436493No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36800.065.243591
GTATCAA61100.053.3367731
ATCAACG78300.041.4867973
TCAACGC78500.041.0788734
TATCAAC79700.040.608752
CAACGCA79550.040.5366635
AACGCAG82150.039.3994456
GTACATG82600.032.745861
ACGCAGA99200.032.2114757
TACATGG82850.032.0991862
CGCAGAG101000.031.5784978
ACATGGG81550.031.0058753
GTGGTAT19250.028.1638011
GCAGAGT113450.027.6934769
TATACCG1306.383688E-527.4597645
GAGTACT69100.025.66087312-13
TGGTATC20200.025.6239992
CAGAGTA107500.025.1022110-11
CATGGGG55350.025.0453554
GTACTTT71650.024.49541914-15
Produced by FastQC (version 0.11.5)