##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364106_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2203418 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.12322083236136 33.0 31.0 34.0 30.0 34.0 2 32.145775790158744 33.0 31.0 34.0 30.0 34.0 3 32.293933334483064 34.0 31.0 34.0 30.0 34.0 4 35.91811494686891 37.0 35.0 37.0 35.0 37.0 5 35.84538839203456 37.0 35.0 37.0 35.0 37.0 6 35.87486169215283 37.0 35.0 37.0 35.0 37.0 7 35.79428914531877 37.0 35.0 37.0 33.0 37.0 8 35.77638968184883 37.0 35.0 37.0 33.0 37.0 9 37.4492642794059 39.0 37.0 39.0 34.0 39.0 10-11 37.369240652477195 39.0 37.0 39.0 34.0 39.0 12-13 37.30820752122384 39.0 37.0 39.0 33.0 39.0 14-15 38.63528209354739 40.0 38.0 41.0 34.0 41.0 16-17 38.605747071141295 40.0 38.0 41.0 34.0 41.0 18-19 38.72234932273405 40.0 38.0 41.0 34.0 41.0 20-21 38.77091977101031 40.0 38.0 41.0 34.0 41.0 22-23 38.74526576437154 40.0 38.0 41.0 34.0 41.0 24-25 38.7176073718196 40.0 38.0 41.0 34.0 41.0 26-27 38.60932287927211 40.0 38.0 41.0 34.0 41.0 28-29 38.54261152445882 40.0 38.0 41.0 34.0 41.0 30-31 38.43754067544152 40.0 38.0 41.0 34.0 41.0 32-33 38.33555412545418 40.0 38.0 41.0 34.0 41.0 34-35 38.233929286227124 40.0 38.0 41.0 33.5 41.0 36-37 38.14830095787545 40.0 38.0 41.0 33.0 41.0 38-39 38.063650428561445 40.0 37.0 41.0 33.0 41.0 40-41 37.953555566851136 40.0 37.0 41.0 33.0 41.0 42-43 37.837542853875206 40.0 37.0 41.0 33.0 41.0 44-45 37.699169199852236 40.0 37.0 41.0 33.0 41.0 46-47 37.49648205651401 40.0 37.0 41.0 32.0 41.0 48-49 37.29800496319808 40.0 37.0 41.0 31.5 41.0 50-51 37.13911795219972 40.0 36.0 41.0 31.0 41.0 52-53 36.993408649652494 40.0 36.0 41.0 31.0 41.0 54-55 36.822355086506505 39.0 36.0 41.0 31.0 41.0 56-57 36.62982171335625 39.0 35.0 41.0 31.0 41.0 58-59 36.42122556863927 39.0 35.0 41.0 30.5 41.0 60-61 36.18276173653841 38.5 35.0 41.0 30.0 41.0 62-63 35.92412129700311 38.0 35.0 40.0 30.0 41.0 64-65 35.71215175695215 37.5 35.0 40.0 29.5 41.0 66-67 35.502294162977705 37.0 35.0 40.0 30.0 41.0 68-69 35.194581554657354 37.0 34.0 39.5 29.0 41.0 70-71 34.88159940601375 36.0 34.0 39.0 29.0 41.0 72-73 34.54575005741081 36.0 34.0 39.0 29.0 40.5 74-75 34.272682033095855 35.0 34.0 37.5 29.0 40.0 76-77 34.07915157269297 35.0 34.0 37.0 29.0 39.0 78-79 33.80035472161887 35.0 34.0 37.0 29.0 39.0 80-81 33.53478368607318 35.0 34.0 36.0 29.0 39.0 82-83 33.28342488806028 35.0 34.0 36.0 29.0 37.0 84-85 33.08157349172967 35.0 34.0 35.5 29.0 37.0 86-87 32.908059886957446 35.0 34.0 35.0 29.0 36.5 88-89 32.76714903844845 35.0 34.0 35.0 29.0 36.0 90-91 32.63907574504701 35.0 34.0 35.0 29.0 36.0 92-93 32.535779865645104 35.0 33.0 35.0 29.0 36.0 94-95 32.45238216262189 35.0 33.0 35.0 29.0 35.5 96-97 32.39257666951981 35.0 33.0 35.0 29.0 35.0 98-99 32.3386248092736 35.0 33.0 35.0 29.0 35.0 100-101 32.27909910874832 35.0 33.0 35.0 28.5 35.0 102-103 31.06432642376526 34.0 31.0 35.0 24.5 35.0 104-105 32.0169532063367 35.0 33.0 35.0 27.0 35.0 106-107 32.16961057774785 35.0 33.0 35.0 27.0 35.0 108-109 32.17172048154276 35.0 33.0 35.0 27.0 35.0 110-111 32.13813221095589 35.0 33.0 35.0 27.0 35.0 112-113 32.08279250691426 35.0 33.0 35.0 27.0 35.0 114-115 32.012500351726274 35.0 33.0 35.0 27.0 35.0 116-117 31.948111524912658 35.0 33.0 35.0 27.0 35.0 118-119 31.86672660384911 35.0 33.0 35.0 27.0 35.0 120-121 31.751386028434005 35.0 33.0 35.0 27.0 35.0 122-123 31.613015097453136 35.0 33.0 35.0 25.0 35.0 124-125 30.741861961733996 34.5 31.5 35.0 22.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.14682516684778335 1101 2 0.2296735550076292 1101 3 0.18308327583580564 1101 4 0.12943849989120793 1101 5 0.07634338230595716 1101 6 0.13551992266720703 1101 7 0.2005518346164621 1101 8 0.13431399128454302 1101 9 0.3305061346428815 1101 10-11 0.30339835056702924 1101 12-13 0.28972090601689615 1101 14-15 0.4298510218927163 1101 16-17 0.37395034154047124 1101 18-19 0.3217357831275365 1101 20-21 0.32582615630654743 1101 22-23 0.31183999194298195 1101 24-25 0.3656737702885309 1101 26-27 0.3355878159071679 1101 28-29 0.3238149290848824 1101 30-31 0.3440294979627865 1101 32-33 0.3560832739824846 1101 34-35 0.42338841751509193 1101 36-37 0.45590408981220065 1101 38-39 0.4473112056284734 1101 40-41 0.40198393976476154 1101 42-43 0.4308740239623816 1101 44-45 0.40567550964387067 1101 46-47 0.3883891279462688 1101 48-49 0.35856735229747017 1101 50-51 0.4541427835186198 1101 52-53 0.3644353811526244 1101 54-55 0.3721205722842882 1101 56-57 0.4552890569504555 1101 58-59 0.48216734465747635 1101 60-61 0.5094772052810086 1101 62-63 0.4291135097158758 1101 64-65 0.4267531510021527 1101 66-67 0.41803632525267886 1101 68-69 0.3791390231320264 1101 70-71 0.368921809541618 1101 72-73 0.36174846754978773 1101 74-75 0.3423471477379678 1101 76-77 0.3656612416365519 1101 78-79 0.26297686852329605 1101 80-81 0.2466452131406882 1101 82-83 0.25364100946642054 1101 84-85 0.25625065721349216 1101 86-87 0.2154716292795129 1101 88-89 0.252079861783038 1101 90-91 0.24163622289093212 1101 92-93 0.2206447734513972 1101 94-95 0.2887649615892798 1101 96-97 0.2724892433205781 1101 98-99 0.3419259305584035 1101 100-101 0.27722677066025625 1101 102-103 0.26511941092756786 1101 104-105 0.26730421998860265 1101 106-107 0.3175379277391599 1101 108-109 0.2669473003035563 1101 110-111 0.19510690975579337 1101 112-113 0.2828434836100797 1101 114-115 0.251977550656143 1101 116-117 0.274901520805507 1101 118-119 0.23106315224192642 1101 120-121 0.29184156683675866 1101 122-123 0.282415645723475 1101 124-125 0.4286175846081193 1102 1 0.20925430491291763 1102 2 0.26329922999924804 1102 3 0.20770916057825417 1102 4 0.15485988991127897 1102 5 0.15597042356809254 1102 6 0.18634888913048542 1102 7 0.22087425133798178 1102 8 0.26002194934495293 1102 9 0.29360987489904034 1102 10-11 0.20929977511907794 1102 12-13 0.25276900009108516 1102 14-15 0.29345742939382546 1102 16-17 0.37100349412691 1102 18-19 0.31058866745626545 1102 20-21 0.29685203187914055 1102 22-23 0.37767216834843254 1102 24-25 0.355210524640718 1102 26-27 0.43893409122311056 1102 28-29 0.35453196442873747 1102 30-31 0.41260900866630124 1102 32-33 0.3189388596982283 1102 34-35 0.38598507214108935 1102 36-37 0.44597232398349007 1102 38-39 0.34768509310895723 1102 40-41 0.38325126000628984 1102 42-43 0.3915325651761705 1102 44-45 0.37815435797168107 1102 46-47 0.42363559595671774 1102 48-49 0.43703517974218187 1102 50-51 0.4233822990872511 1102 52-53 0.5347589881929977 1102 54-55 0.5289872572580379 1102 56-57 0.5738457948458731 1102 58-59 0.5239381805522783 1102 60-61 0.5288013216603034 1102 62-63 0.5079116383974736 1102 64-65 0.46086318290171135 1102 66-67 0.45188752403112886 1102 68-69 0.4885066953846291 1102 70-71 0.44812611616423226 1102 72-73 0.4054456116368499 1102 74-75 0.45552129315025525 1102 76-77 0.37878516832896736 1102 78-79 0.3430264974720103 1102 80-81 0.28275336423848785 1102 82-83 0.32146586781112774 1102 84-85 0.3968744371978872 1102 86-87 0.3582745744908493 1102 88-89 0.3141666974540769 1102 90-91 0.36416867475477943 1102 92-93 0.3612139849087441 1102 94-95 0.3358724916639346 1102 96-97 0.3256849359534044 1102 98-99 0.21632034971660374 1102 100-101 0.24005384750459768 1102 102-103 0.35563659614382814 1102 104-105 0.34397853571935144 1102 106-107 0.3028263214249307 1102 108-109 0.2271779635247242 1102 110-111 0.15916408072153843 1102 112-113 0.25258503942442445 1102 114-115 0.3102915653919922 1102 116-117 0.24274699175001757 1102 118-119 0.2843207120647513 1102 120-121 0.3315719610850536 1102 122-123 0.3151220810574671 1102 124-125 0.567882147816448 1103 1 0.13971278910180018 1103 2 0.20945185631572372 1103 3 0.13409184292529375 1103 4 0.04483696005880233 1103 5 0.06499130337277848 1103 6 0.091497977168153 1103 7 0.08539415432368003 1103 8 0.12254039202787936 1103 9 0.18172005679033276 1103 10-11 0.10424764208040926 1103 12-13 0.11999899471776132 1103 14-15 0.05249350713609857 1103 16-17 0.16323319703735706 1103 18-19 0.19818624175447752 1103 20-21 0.21850427246067738 1103 22-23 0.27399162765765084 1103 24-25 0.157588891882952 1103 26-27 0.28137987456536706 1103 28-29 0.25497782160868354 1103 30-31 0.2203855130947403 1103 32-33 0.260199309117219 1103 34-35 0.17230030013023878 1103 36-37 0.22505045477297614 1103 38-39 0.3140556630153597 1103 40-41 0.31121528345606464 1103 42-43 0.24393773205410696 1103 44-45 0.2496864019001208 1103 46-47 0.2707693548323036 1103 48-49 0.2569206815983591 1103 50-51 0.30796214067289895 1103 52-53 0.20656445153115754 1103 54-55 0.2076495345996392 1103 56-57 0.2862886686773578 1103 58-59 0.2195386502058554 1103 60-61 0.1743233435579512 1103 62-63 0.310691282840331 1103 64-65 0.32446671433236673 1103 66-67 0.18144142008794972 1103 68-69 0.11589812290615953 1103 70-71 0.12245286857060478 1103 72-73 0.14704418408280873 1103 74-75 0.11081432641183397 1103 76-77 0.0878190271149819 1103 78-79 0.06260699804941794 1103 80-81 0.09785223308727353 1103 82-83 0.1118553673274505 1103 84-85 0.05778798149658115 1103 86-87 -0.03827228001429006 1103 88-89 -0.061995504806468205 1103 90-91 -0.050056327028997316 1103 92-93 -0.05802628845901836 1103 94-95 -0.03352242000151762 1103 96-97 -0.06417104804562257 1103 98-99 -0.03289230338420879 1103 100-101 0.05433399800340055 1103 102-103 0.13864007971389114 1103 104-105 0.10772932167940752 1103 106-107 0.12289464806217865 1103 108-109 0.1495959353106997 1103 110-111 0.009248381931072913 1103 112-113 0.017427417969564374 1103 114-115 -0.007327588255996886 1103 116-117 0.03526711969197294 1103 118-119 0.07662621337384223 1103 120-121 0.13180917898828426 1103 122-123 0.18582192073787596 1103 124-125 0.18018151638948865 1104 1 0.10461648951808655 1104 2 0.15319560196178372 1104 3 0.0917162252685415 1104 4 0.09004512116741381 1104 5 0.08790429381613762 1104 6 0.1716866432307853 1104 7 0.15778284568786205 1104 8 0.11114016386681413 1104 9 0.1947200767260142 1104 10-11 0.052632907861223543 1104 12-13 0.11872553326320912 1104 14-15 0.10736097119272614 1104 16-17 0.233648488933909 1104 18-19 0.1635755250406845 1104 20-21 0.19858475485197147 1104 22-23 0.24440884791208362 1104 24-25 0.2650893935354688 1104 26-27 0.2314626076390951 1104 28-29 0.28645048522611916 1104 30-31 0.251123164943607 1104 32-33 0.18254335946244993 1104 34-35 0.15240314029396274 1104 36-37 0.2712802729241659 1104 38-39 0.2447536484496453 1104 40-41 0.28713060600139784 1104 42-43 0.37718139208853074 1104 44-45 0.32565238343148906 1104 46-47 0.23447891730746306 1104 48-49 0.25956868027030566 1104 50-51 0.30169823809764296 1104 52-53 0.26955216537626114 1104 54-55 0.26095153537806226 1104 56-57 0.20362145722629776 1104 58-59 0.2829166469159077 1104 60-61 0.2594919239567801 1104 62-63 0.22754580137409164 1104 64-65 0.2033682620677908 1104 66-67 0.20194918000497353 1104 68-69 0.13444659669940506 1104 70-71 0.035841373433697754 1104 72-73 0.10208535871545621 1104 74-75 0.17775270522366782 1104 76-77 0.11050643695027418 1104 78-79 0.1850006640179629 1104 80-81 0.18345175954964787 1104 82-83 0.08947882368629223 1104 84-85 0.14601240359939283 1104 86-87 0.18992339558262472 1104 88-89 0.2125103837650002 1104 90-91 0.21162081145933342 1104 92-93 0.21013520955645504 1104 94-95 0.1967759575135659 1104 96-97 0.22331254926778854 1104 98-99 0.2220087998041791 1104 100-101 0.21310119788429915 1104 102-103 0.2050121082029115 1104 104-105 0.2183554864960584 1104 106-107 0.17288974798371726 1104 108-109 0.1496879282285022 1104 110-111 0.17611156410164597 1104 112-113 0.24712194931431952 1104 114-115 0.2557310276749192 1104 116-117 0.20816881014452093 1104 118-119 0.21414030109940185 1104 120-121 0.18730852958815092 1104 122-123 0.2524439370698559 1104 124-125 0.34702387788448164 1105 1 0.04985347040228305 1105 2 0.09654240017540161 1105 3 0.025603030208642963 1105 4 0.004878492242035293 1105 5 0.06043890963781706 1105 6 0.08846183329232815 1105 7 0.13160039009394353 1105 8 0.061403201830330545 1105 9 0.0921082604841601 1105 10-11 0.08309484928216193 1105 12-13 0.11446351940468702 1105 14-15 0.13135447804058487 1105 16-17 0.147235205248478 1105 18-19 0.18088616444227057 1105 20-21 0.2634752789825967 1105 22-23 0.2547473922944121 1105 24-25 0.2516197898538266 1105 26-27 0.21847919243514013 1105 28-29 0.14847058963786708 1105 30-31 0.21295759873581233 1105 32-33 0.18481920946913277 1105 34-35 0.2863468735849608 1105 36-37 0.2382193188666477 1105 38-39 0.297205919479957 1105 40-41 0.26652711962371 1105 42-43 0.2668670516562628 1105 44-45 0.3204894396698563 1105 46-47 0.3433930697762122 1105 48-49 0.34870607772108997 1105 50-51 0.36149341343136854 1105 52-53 0.33154055426724227 1105 54-55 0.38610911556637006 1105 56-57 0.3707921970628192 1105 58-59 0.4471332483309638 1105 60-61 0.3555674813698957 1105 62-63 0.44345943537019394 1105 64-65 0.3503267775612997 1105 66-67 0.3223458551398082 1105 68-69 0.21638604756964241 1105 70-71 0.16930552182564895 1105 72-73 0.20304829805635904 1105 74-75 0.2448712492739915 1105 76-77 0.2602206166404315 1105 78-79 0.27845423384378876 1105 80-81 0.24228785680165288 1105 82-83 0.18093724145600731 1105 84-85 0.21973434286133653 1105 86-87 0.22759185906470236 1105 88-89 0.2423837353810825 1105 90-91 0.2799266239200904 1105 92-93 0.19834594149068607 1105 94-95 0.18650340305948987 1105 96-97 0.18930141361643393 1105 98-99 0.24182517629400735 1105 100-101 0.19286132105571596 1105 102-103 0.228682727569975 1105 104-105 0.24995686624043856 1105 106-107 0.21269075464425669 1105 108-109 0.237209191522453 1105 110-111 0.1917075659649612 1105 112-113 0.16528210242196195 1105 114-115 0.21631674308874693 1105 116-117 0.1705121780741834 1105 118-119 0.1987228710086022 1105 120-121 0.18555826024010003 1105 122-123 0.19953418478568352 1105 124-125 0.24128590916912174 1106 1 0.04942582261607242 1106 2 0.08303828221269072 1106 3 0.05092592761846504 1106 4 -5.022391784095248E-4 1106 5 0.0331358767146952 1106 6 0.06113272428468264 1106 7 0.02724252739962907 1106 8 0.08181473734114775 1106 9 0.1270425281647789 1106 10-11 0.18082403404768854 1106 12-13 0.14314227165031923 1106 14-15 0.1388586261966651 1106 16-17 0.14982456044180736 1106 18-19 0.19818833273732395 1106 20-21 0.14535541957386044 1106 22-23 0.1956701014074511 1106 24-25 0.13610822511034115 1106 26-27 0.14580500762158266 1106 28-29 0.1611520757550906 1106 30-31 0.17458183686810003 1106 32-33 0.20942034830780898 1106 34-35 0.26890691376957676 1106 36-37 0.2609941710907293 1106 38-39 0.24181304209381693 1106 40-41 0.29103520801339755 1106 42-43 0.15234431948647398 1106 44-45 0.287980522335161 1106 46-47 0.31711180763881686 1106 48-49 0.41501097302560197 1106 50-51 0.30721384761189796 1106 52-53 0.28771187139772536 1106 54-55 0.24951393854406945 1106 56-57 0.2861464818438577 1106 58-59 0.3624226173857821 1106 60-61 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2216 110-111 -0.778135148190767 2216 112-113 -0.7400570122958072 2216 114-115 -0.9150256027667965 2216 116-117 -0.9515367779959618 2216 118-119 -1.0051382457765996 2216 120-121 -1.1336597363306815 2216 122-123 -1.1611067503872974 2216 124-125 -1.6281454848625572 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 12.0 4 37.0 5 105.0 6 278.0 7 678.0 8 1293.0 9 2212.0 10 3326.0 11 4640.0 12 5922.0 13 8147.0 14 11825.0 15 10837.0 16 5734.0 17 4926.0 18 4670.0 19 4390.0 20 4403.0 21 4716.0 22 5481.0 23 6287.0 24 8146.0 25 11574.0 26 15276.0 27 20326.0 28 26814.0 29 34904.0 30 44365.0 31 56216.0 32 71578.0 33 93056.0 34 127243.0 35 188273.0 36 357557.0 37 721480.0 38 334371.0 39 2312.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.41402297763699 18.587409942428618 13.264875637813086 24.7336914421213 2 16.25161513147295 20.014740982154244 38.830234081168896 24.90340980520391 3 19.56800752285767 23.820537002057712 29.530120930300107 27.081334544784514 4 13.375265156225463 15.955982931972054 36.05416675365273 34.614585158149744 5 13.599189985740336 37.74249824590704 34.43264056116452 14.225671207188107 6 33.57969300423251 35.80028846092752 16.49042533010078 14.129593204739182 7 28.633559315572445 32.20246907304924 20.70192764150969 18.46204396986863 8 26.28411858303781 35.856746200675495 19.15455896248465 18.704576253802045 9 26.360841529697527 15.284903077766524 19.35767009044139 38.996585302094566 10-11 25.409439697377813 26.01196101946644 27.544473729893358 21.03412555326239 12-13 25.620328713343127 23.29230751774337 28.062406744467072 23.024957024446422 14-15 22.786648467664435 24.94767786858369 25.52909287504524 26.736580788706632 16-17 22.673429519115714 28.05034651979126 26.280770778196246 22.99545318289678 18-19 22.58817891112807 26.879148668114723 28.164016087732787 22.36865633302442 20-21 23.159813526076306 26.085676889269312 28.419051673354762 22.335457911299628 22-23 23.0359151100699 25.86905888941635 28.27368660871428 22.821339391799466 24-25 22.824470890226003 26.06293948765055 28.31920679598696 22.793382826136487 26-27 22.669824790393832 26.254573576143976 28.322587906606916 22.753013726855276 28-29 22.689544153673975 26.408221227202468 28.291477150499816 22.610757468623746 30-31 22.990304154726886 26.13662046874447 28.207244381229522 22.66583099529912 32-33 22.75122105746617 26.148057245606598 28.30668080228082 22.794040894646407 34-35 22.974192822242536 26.229430820661353 28.202479057536973 22.59389729955914 36-37 23.04335809183732 26.145129975338318 28.09221854409831 22.71929338872606 38-39 22.85757854388046 26.27628983697147 28.11493325369948 22.751198365448587 40-41 23.099634295444623 26.14102272015569 28.018582947039555 22.74076003736014 42-43 22.862820399942272 26.1654847151108 27.940658558657503 23.031036326289428 44-45 22.85730623966946 26.283778202774055 27.924252229944567 22.934663327611922 46-47 22.92942147155011 26.38242040320992 27.543911323226006 23.144246802013964 48-49 23.134466542435433 26.431798233471817 27.431517760134483 23.002217463958267 50-51 23.09121555692111 26.587352014007326 27.324638357315774 22.99679407175579 52-53 23.08336411883719 26.55903237606301 27.161233138696332 23.196370366403468 54-55 23.049666472725555 26.442168485507516 27.247031657179893 23.261133384587037 56-57 22.980319666990106 26.652183108243648 27.18989315690441 23.17760406786184 58-59 23.148580977372426 26.47802187328959 27.306961275618153 23.06643587371983 60-61 23.142068368325937 26.423447571001052 27.38853907883116 23.04594498184185 62-63 22.981113887605527 26.510925298785796 27.40850805430472 23.099452759303954 64-65 22.90845404730287 26.383963460405607 27.392986714277544 23.314595778013977 66-67 22.87221489522188 26.536816890848673 27.226881145565663 23.364087068363787 68-69 22.65875108581304 26.42555792863633 27.4273424288991 23.488348556651527 70-71 22.828805065584472 26.28171322917395 27.32393490477068 23.565546800470905 72-73 22.90305334711798 26.449293779028764 27.066380505196925 23.581272368656332 74-75 22.662064120380247 26.616670100725326 27.05147184964451 23.66979392924992 76-77 22.91097286125465 26.441442340944842 26.96946289809741 23.678121899703097 78-79 22.868289176179918 26.485079090758084 27.014007328614 23.632624404448 80-81 22.77888262690057 26.577526370393635 26.932497601453743 23.711093401252054 82-83 22.864249997050038 26.535704981987074 27.005497822020153 23.594547198942735 84-85 22.91848391907482 26.426715221533094 26.833651172859618 23.82114968653247 86-87 22.646429320265153 26.33100029136551 27.120228662922784 23.902341725446558 88-89 22.78430601910305 26.273339874685604 27.050178404642246 23.89217570156911 90-91 22.812466812924285 26.394197560335808 26.89993455622129 23.89340107051862 92-93 22.812653525716303 26.103223742209547 26.964522157058298 24.119600575015856 94-95 22.67074762226018 26.267770503432075 26.932219239717792 24.129262634589956 96-97 22.59631968222039 26.199911223589474 27.00330142949991 24.200467664690226 98-99 22.531188616699527 26.045989594112285 26.92192134949768 24.50090043969051 100-101 22.432352700899543 26.114376355896045 27.04155963219476 24.411711311009647 102-103 22.50186479229039 25.96428539820549 26.989189310627083 24.54466049887704 104-105 22.456852718149968 26.004405903193135 26.84970659462536 24.68903478403154 106-107 22.490016077768832 25.93597852424057 26.929241476104405 24.64476392188619 108-109 22.487328459538798 25.85368765603228 26.911694042702027 24.747289841726893 110-111 22.328923971126756 26.090661812850435 26.81144898443201 24.76896523159081 112-113 22.372448950641033 26.154249469731273 26.755413620955178 24.71788795867252 114-115 22.358084807397365 26.078693467541992 26.637086649792984 24.92613507526766 116-117 22.125567416670126 25.91378512399696 26.834725729978327 25.125921729354587 118-119 22.281142005352027 25.885580227221816 26.674313503831776 25.158964263594374 120-121 22.319466094824275 25.95629109852388 26.689720727501182 25.034522079150662 122-123 22.151980322282093 25.962586372572115 26.65499675230505 25.230436552840747 124-125 22.084050976270518 25.95590720090999 26.577916107890527 25.38212571492896 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 258.0 1 186.5 2 149.5 3 205.5 4 158.5 5 115.0 6 261.5 7 629.0 8 1013.5 9 1167.0 10 1000.5 11 787.0 12 835.5 13 993.5 14 1160.5 15 1337.0 16 1527.5 17 1726.0 18 1934.5 19 2171.0 20 2432.5 21 2785.0 22 3316.0 23 4084.5 24 5354.0 25 7367.5 26 9772.0 27 12298.5 28 15034.0 29 18253.5 30 21811.5 31 25562.0 32 29180.5 33 32919.0 34 36874.0 35 41173.0 36 46964.5 37 51429.5 38 53961.5 39 56914.5 40 60453.5 41 65109.0 42 68351.0 43 71098.0 44 76214.0 45 80374.5 46 83104.0 47 85501.5 48 85690.5 49 84901.5 50 84836.5 51 83596.5 52 82222.5 53 82896.5 54 83298.5 55 79338.5 56 72581.5 57 66286.5 58 61257.0 59 54652.0 60 45159.5 61 36350.0 62 28559.5 63 21885.5 64 17004.5 65 13148.5 66 9672.5 67 7486.0 68 5753.5 69 3749.5 70 2448.0 71 1717.0 72 1198.5 73 852.5 74 659.5 75 488.0 76 284.5 77 120.0 78 58.5 79 31.0 80 15.0 81 11.5 82 8.0 83 4.5 84 3.0 85 2.5 86 1.5 87 0.5 88 0.0 89 1.0 90 2.0 91 1.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16855630661091087 2 0.0016792093011857034 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 9.076807033436234E-5 10-11 4.765323692554023E-4 12-13 0.005922616589317142 14-15 2.2692017583590585E-5 16-17 3.8576429892103997E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 2.2692017583590585E-5 94-95 5.673004395897646E-4 96-97 0.005899924571733552 98-99 0.0022692017583590585 100-101 0.0035399547430401313 102-103 0.004061871147462715 104-105 0.010778708352205528 106-107 0.01706439722286012 108-109 0.024893143289198873 110-111 0.015453263974425188 112-113 0.023849310480353703 114-115 0.02630004837938149 116-117 0.025483135746372225 118-119 0.014908655552419015 120-121 0.02178433688024696 122-123 0.01438673914799643 124-125 0.014273279060078478 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2203418.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.77837512575452 #Duplication Level Percentage of deduplicated Percentage of total 1 84.26634693055284 63.013005012498546 2 10.218641621978392 15.282668329678978 3 2.8740125716987315 6.447419706078667 4 1.1305952466105809 3.381763018657638 5 0.5207752639600026 1.9471364022307451 6 0.28667074068868725 1.2862063310877934 7 0.16644916927014552 0.8712758893337209 8 0.12032233097608236 0.7198006721387744 9 0.07542755679911475 0.5076315123429024 >10 0.3208178629245551 3.9377588516865147 >50 0.013346446262978755 0.6855735554150134 >100 0.00606247093632631 0.9032572852892584 >500 1.0635746831841958E-4 0.055831735416649125 >1k 4.254298732736783E-4 0.9606716981448786 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4300 0.19515135121887903 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4136 0.18770836945146133 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2329 0.10569941790436493 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 4.538403516718117E-5 0.0 16-17 0.0 0.0 0.0 4.538403516718117E-5 0.0 18-19 2.2692017583590585E-5 0.0 0.0 9.076807033436234E-5 0.0 20-21 4.538403516718117E-5 0.0 0.0 9.076807033436234E-5 0.0 22-23 4.538403516718117E-5 0.0 0.0 1.8153614066872468E-4 0.0 24-25 4.538403516718117E-5 0.0 0.0 2.7230421100308703E-4 0.0 26-27 4.538403516718117E-5 0.0 0.0 4.311483340882211E-4 0.0 28-29 4.538403516718117E-5 0.0 0.0 0.0013842130725990258 0.0 30-31 4.538403516718117E-5 0.0 0.0 0.005741080448648418 0.0 32-33 4.538403516718117E-5 0.0 0.0 0.01629286862501804 0.0 34-35 4.538403516718117E-5 2.2692017583590585E-5 0.0 0.033629570058881245 0.0 36-37 4.538403516718117E-5 6.807605275077176E-5 0.0 0.0614726756339469 0.0 38-39 4.538403516718117E-5 1.1346008791795293E-4 0.0 0.10277214763608175 0.0 40-41 4.538403516718117E-5 1.3615210550154352E-4 0.0 0.1455919848163172 0.0 42-43 4.538403516718117E-5 1.3615210550154352E-4 0.0 0.19142986033517018 0.0 44-45 9.076807033436234E-5 1.3615210550154352E-4 0.0 0.25047449008767286 0.0 46-47 1.1346008791795293E-4 1.3615210550154352E-4 0.0 0.3232250984606643 0.0 48-49 1.3615210550154352E-4 1.3615210550154352E-4 0.0 0.39967450569978097 0.0 50-51 1.3615210550154352E-4 1.3615210550154352E-4 0.0 0.48563186830642213 0.0 52-53 1.3615210550154352E-4 1.3615210550154352E-4 0.0 0.5686846526623637 0.0 54-55 1.3615210550154352E-4 1.588441230851341E-4 0.0 0.6649214992343713 0.0 56-57 2.2692017583590584E-4 1.8153614066872468E-4 0.0 0.7815130855788597 0.0 58-59 2.2692017583590584E-4 1.8153614066872468E-4 0.0 0.9145563846714513 0.0 60-61 2.2692017583590584E-4 1.8153614066872468E-4 0.0 1.06085182203286 0.0 62-63 2.2692017583590584E-4 1.8153614066872468E-4 0.0 1.2062395786909246 0.0 64-65 2.2692017583590584E-4 1.8153614066872468E-4 0.0 1.3614983629978514 0.0 66-67 2.2692017583590584E-4 1.8153614066872468E-4 0.0 1.5516574703483408 0.0 68-69 2.2692017583590584E-4 1.8153614066872468E-4 0.0 1.7702042916959015 0.0 70-71 2.2692017583590584E-4 1.8153614066872468E-4 0.0 2.005792818248739 0.0 72-73 2.2692017583590584E-4 1.8153614066872468E-4 0.0 2.2516608287669433 0.0 74-75 2.2692017583590584E-4 1.8153614066872468E-4 0.0 2.4986180561291595 0.0 76-77 2.2692017583590584E-4 1.8153614066872468E-4 0.0 2.772851088626852 0.0 78-79 2.2692017583590584E-4 1.8153614066872468E-4 0.0 3.087702832599171 0.0 80-81 2.2692017583590584E-4 1.8153614066872468E-4 0.0 3.43427801715335 0.0 82-83 2.2692017583590584E-4 1.8153614066872468E-4 0.0 3.795625705154446 0.0 84-85 2.2692017583590584E-4 1.8153614066872468E-4 0.0 4.163939842553706 0.0 86-87 2.496121934194964E-4 1.8153614066872468E-4 0.0 4.5503622099846694 0.0 88-89 2.7230421100308703E-4 1.8153614066872468E-4 0.0 4.984324354253255 0.0 90-91 2.7230421100308703E-4 1.8153614066872468E-4 0.0 5.465871659394631 0.0 92-93 2.7230421100308703E-4 1.8153614066872468E-4 0.0 5.972266723790039 0.0 94-95 2.7230421100308703E-4 1.8153614066872468E-4 0.0 6.479297164677787 0.0 96-97 2.7230421100308703E-4 1.8153614066872468E-4 0.0 6.988120274954639 0.0 98-99 2.7230421100308703E-4 1.8153614066872468E-4 0.0 7.5412835875898265 0.0 100-101 2.9499622858667764E-4 1.8153614066872468E-4 0.0 8.153877294276437 0.0 102-103 3.176882461702682E-4 1.8153614066872468E-4 0.0 8.795380631364544 0.0 104-105 3.6307228133744936E-4 1.8153614066872468E-4 0.0 9.441013915652864 0.0 106-107 4.084563165046305E-4 1.8153614066872468E-4 0.0 10.086079899501591 0.0 108-109 4.084563165046305E-4 1.8153614066872468E-4 0.0 10.746258767060993 0.0 110-111 4.311483340882211E-4 1.8153614066872468E-4 0.0 11.45463547996794 0.0 112-113 4.538403516718117E-4 1.8153614066872468E-4 0.0 12.203267832068178 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3680 0.0 65.24359 1 GTATCAA 6110 0.0 53.336773 1 ATCAACG 7830 0.0 41.486797 3 TCAACGC 7850 0.0 41.078873 4 TATCAAC 7970 0.0 40.60875 2 CAACGCA 7955 0.0 40.536663 5 AACGCAG 8215 0.0 39.399445 6 GTACATG 8260 0.0 32.74586 1 ACGCAGA 9920 0.0 32.211475 7 TACATGG 8285 0.0 32.099186 2 CGCAGAG 10100 0.0 31.578497 8 ACATGGG 8155 0.0 31.005875 3 GTGGTAT 1925 0.0 28.163801 1 GCAGAGT 11345 0.0 27.693476 9 TATACCG 130 6.383688E-5 27.459764 5 GAGTACT 6910 0.0 25.660873 12-13 TGGTATC 2020 0.0 25.623999 2 CAGAGTA 10750 0.0 25.10221 10-11 CATGGGG 5535 0.0 25.045355 4 GTACTTT 7165 0.0 24.495419 14-15 >>END_MODULE