FastQCFastQC Report
Thu 26 May 2016
SRR1364105_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364105_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2630235
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN107680.4093930770444466No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25600.052.770291
GTATCAA41700.046.8102951
TCAACGC45250.041.1777084
ATCAACG45100.041.042823
CAACGCA46850.039.9045945
AACGCAG49650.037.5349856
TATCAAC53050.035.2267652
GTACATG89750.033.1542431
TACATGG89550.032.1668322
ACATGGG90950.031.3462923
CGCAGAG59200.030.878258
ACGCAGA60250.030.7354377
GAGTACA66500.025.4155941
GCAGAGT71150.024.2694059
AGTACAT67450.023.6472852
CATGGGG44800.023.5196934
TAGACCG1852.6785277E-522.5283375
CAGAGTA68150.020.79423710-11
AGAGTAC61250.020.65783110-11
CATGGGA59700.020.4416484
Produced by FastQC (version 0.11.5)