##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364105_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2630235 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.102093158976288 31.0 31.0 34.0 28.0 34.0 2 31.127617874448482 33.0 31.0 34.0 28.0 34.0 3 31.23959570152477 33.0 31.0 34.0 28.0 34.0 4 34.71720891859473 37.0 35.0 37.0 32.0 37.0 5 34.78125262571595 37.0 35.0 37.0 32.0 37.0 6 34.81588755377371 37.0 35.0 37.0 32.0 37.0 7 34.737914292829345 37.0 35.0 37.0 32.0 37.0 8 34.7177024106211 37.0 35.0 37.0 32.0 37.0 9 36.25561822422711 39.0 37.0 39.0 32.0 39.0 10-11 36.178794100146945 39.0 37.0 39.0 32.0 39.0 12-13 36.0894507524993 39.0 37.0 39.0 31.0 39.0 14-15 37.36224614910835 40.0 38.0 41.0 31.0 41.0 16-17 37.36871990525562 40.0 38.0 41.0 31.0 41.0 18-19 37.37084519063886 40.0 38.0 41.0 31.0 41.0 20-21 37.39017635306351 40.0 38.0 41.0 31.0 41.0 22-23 37.34246654766589 40.0 38.0 41.0 31.0 41.0 24-25 37.34723741414741 40.0 38.0 41.0 31.0 41.0 26-27 37.309681074124555 40.0 37.5 41.0 31.0 41.0 28-29 37.265860084745285 40.0 37.0 41.0 31.0 41.0 30-31 37.221815921391055 40.0 37.0 41.0 31.0 41.0 32-33 37.12640695603244 40.0 37.0 41.0 30.5 41.0 34-35 37.105463960444595 40.0 37.0 41.0 30.0 41.0 36-37 37.0458822120457 40.0 37.0 41.0 30.0 41.0 38-39 36.97192342129125 40.0 37.0 41.0 30.0 41.0 40-41 36.90591810237488 40.0 37.0 41.0 30.0 41.0 42-43 36.80421578300037 40.0 37.0 41.0 30.0 41.0 44-45 36.71628656755004 40.0 37.0 41.0 30.0 41.0 46-47 36.61805218925305 40.0 36.0 41.0 30.0 41.0 48-49 36.52272363496037 40.0 36.0 41.0 30.0 41.0 50-51 36.41144707602172 40.0 36.0 41.0 30.0 41.0 52-53 36.290540008782486 40.0 36.0 41.0 29.0 41.0 54-55 36.128211357540295 39.5 35.0 41.0 29.0 41.0 56-57 35.958178831929466 39.0 35.0 41.0 28.5 41.0 58-59 35.76517611544216 39.0 35.0 41.0 28.0 41.0 60-61 35.53885527338812 39.0 35.0 41.0 28.0 41.0 62-63 35.29192866797074 38.0 35.0 40.5 28.0 41.0 64-65 35.0252954583906 38.0 34.0 40.0 28.0 41.0 66-67 34.72620355215408 37.0 34.0 40.0 27.0 41.0 68-69 34.40499404045646 37.0 34.0 40.0 26.5 41.0 70-71 34.06387071877607 36.0 34.0 39.0 26.0 41.0 72-73 33.707373295542034 36.0 33.5 39.0 26.0 41.0 74-75 33.42562774809095 35.0 33.0 38.5 26.0 40.0 76-77 33.18657781529027 35.0 33.0 37.0 26.0 39.5 78-79 32.8680598881849 35.0 33.0 37.0 26.0 39.0 80-81 32.63864730717978 35.0 33.0 36.5 26.0 39.0 82-83 32.514207095563705 35.0 33.0 36.0 26.0 38.0 84-85 32.2806475467021 35.0 33.0 36.0 26.0 37.0 86-87 32.06633608784006 35.0 33.0 35.5 26.0 37.0 88-89 31.869190395535 35.0 33.0 35.0 25.5 36.0 90-91 31.71228920609755 35.0 33.0 35.0 25.0 36.0 92-93 31.583133256153918 35.0 33.0 35.0 25.0 36.0 94-95 31.471700247316303 35.0 33.0 35.0 25.0 36.0 96-97 31.379543272749395 35.0 33.0 35.0 24.5 35.5 98-99 31.29696186842621 35.0 33.0 35.0 24.0 35.0 100-101 31.217452813151674 35.0 33.0 35.0 24.0 35.0 102-103 30.115215180392624 34.0 30.5 35.0 19.5 35.0 104-105 30.93237619452254 35.0 32.5 35.0 23.0 35.0 106-107 31.022866397869393 35.0 33.0 35.0 23.0 35.0 108-109 30.991326250316035 35.0 33.0 35.0 21.5 35.0 110-111 30.912381973473856 35.0 33.0 35.0 20.0 35.0 112-113 30.824940737234506 35.0 33.0 35.0 19.0 35.0 114-115 30.716989166367263 35.0 33.0 35.0 18.5 35.0 116-117 30.61270361773758 35.0 33.0 35.0 16.5 35.0 118-119 30.475700650322118 35.0 32.0 35.0 7.5 35.0 120-121 30.32542814615424 35.0 32.0 35.0 2.0 35.0 122-123 30.148769596633002 35.0 32.0 35.0 2.0 35.0 124-125 29.306556258281105 34.5 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2574198104779555 1101 2 0.26015700143802434 1101 3 0.3477345270395489 1101 4 0.28231247339985543 1101 5 0.3344813914768139 1101 6 0.23291276581914389 1101 7 0.2594508913475764 1101 8 0.27789899709355126 1101 9 0.22819343151338956 1101 10-11 0.3132306973119299 1101 12-13 0.33268910036863275 1101 14-15 0.3831111005738279 1101 16-17 0.4123930202949566 1101 18-19 0.3913834852648108 1101 20-21 0.43357431084545794 1101 22-23 0.45329276988518785 1101 24-25 0.47276408523933355 1101 26-27 0.4293573270172786 1101 28-29 0.47229841174191023 1101 30-31 0.3921334943093342 1101 32-33 0.35900364044987754 1101 34-35 0.36177707316940655 1101 36-37 0.4045639566453261 1101 38-39 0.3607649966132698 1101 40-41 0.456426546941735 1101 42-43 0.4394903937912886 1101 44-45 0.43369514302686696 1101 46-47 0.4244914570517153 1101 48-49 0.4071114325479641 1101 50-51 0.35392226759326917 1101 52-53 0.37345485920359067 1101 54-55 0.3427893566524034 1101 56-57 0.4050747727805941 1101 58-59 0.38600894022103205 1101 60-61 0.3631591038501938 1101 62-63 0.4428172357614031 1101 64-65 0.4919662981342725 1101 66-67 0.45284071967376605 1101 68-69 0.48349948767728534 1101 70-71 0.5357166128942765 1101 72-73 0.5837839073102487 1101 74-75 0.5479003798307431 1101 76-77 0.526918827418001 1101 78-79 0.5268550389819566 1101 80-81 0.45513161492412024 1101 82-83 0.4197866650241693 1101 84-85 0.43321591347158517 1101 86-87 0.38483443430946807 1101 88-89 0.4015269335139493 1101 90-91 0.4002220648362176 1101 92-93 0.41573134806231593 1101 94-95 0.41401214934500885 1101 96-97 0.38138829655005324 1101 98-99 0.4170371746862429 1101 100-101 0.45477534527556074 1101 102-103 0.4855049459406082 1101 104-105 0.45388036959265676 1101 106-107 0.46755058891177725 1101 108-109 0.47079232809430493 1101 110-111 0.43832536372226727 1101 112-113 0.4581454992292997 1101 114-115 0.4469439347577797 1101 116-117 0.4826033425432712 1101 118-119 0.5245431166566625 1101 120-121 0.5607756881698407 1101 122-123 0.5368745487106246 1101 124-125 0.5557798242127738 1102 1 0.44890142295334456 1102 2 0.4068515045866299 1102 3 0.43831885338693866 1102 4 0.438553657547466 1102 5 0.41208088551936584 1102 6 0.46498659155027156 1102 7 0.42396496921217164 1102 8 0.45017412006279756 1102 9 0.442390976475707 1102 10-11 0.4635859781644882 1102 12-13 0.49988401557885 1102 14-15 0.5093327620440959 1102 16-17 0.6105178500256017 1102 18-19 0.5311406396369733 1102 20-21 0.5991485499900477 1102 22-23 0.6078958819446783 1102 24-25 0.6635961039100664 1102 26-27 0.7111381347149646 1102 28-29 0.7143719976996366 1102 30-31 0.7181624903838042 1102 32-33 0.6972794363499233 1102 34-35 0.6712717012847804 1102 36-37 0.6674371022168799 1102 38-39 0.6597573364579716 1102 40-41 0.7641383851378052 1102 42-43 0.68148155319669 1102 44-45 0.7649657847949527 1102 46-47 0.7145137815914921 1102 48-49 0.7192312236220886 1102 50-51 0.7500906033175809 1102 52-53 0.8004981109805271 1102 54-55 0.7580612998848792 1102 56-57 0.7128253162373142 1102 58-59 0.7398699714017454 1102 60-61 0.7859682918244957 1102 62-63 0.7376819156955676 1102 64-65 0.8083031127354303 1102 66-67 0.7895048754789471 1102 68-69 0.7635552787491378 1102 70-71 0.8296287805546569 1102 72-73 0.787750919044278 1102 74-75 0.7395906378518191 1102 76-77 0.7759895632028702 1102 78-79 0.7384730988850308 1102 80-81 0.7570157855895587 1102 82-83 0.7155233517129815 1102 84-85 0.68055654545023 1102 86-87 0.684637663594664 1102 88-89 0.6517973545055256 1102 90-91 0.6346763708213707 1102 92-93 0.6518551030104831 1102 94-95 0.717819096327414 1102 96-97 0.7620339728700536 1102 98-99 0.7078074214685692 1102 100-101 0.7074193450316599 1102 102-103 0.7621871181908304 1102 104-105 0.7217440391345384 1102 106-107 0.780237653962299 1102 108-109 0.7717496002613728 1102 110-111 0.7487228062721698 1102 112-113 0.795768321729323 1102 114-115 0.788727252605451 1102 116-117 0.7930612976376281 1102 118-119 0.8316889971398034 1102 120-121 0.9295635738786103 1102 122-123 0.8983909681723397 1102 124-125 0.9745031169603671 1103 1 0.3187460764759926 1103 2 0.38057289811670003 1103 3 0.3410970757605192 1103 4 0.3037073033167985 1103 5 0.36252409188784185 1103 6 0.31574641667577197 1103 7 0.3752063828051604 1103 8 0.3245665928068959 1103 9 0.34293332369234264 1103 10-11 0.3695600928445941 1103 12-13 0.41320032875226786 1103 14-15 0.4633390222742406 1103 16-17 0.40149119678837053 1103 18-19 0.4108148072486486 1103 20-21 0.36363697488383195 1103 22-23 0.5170860592389204 1103 24-25 0.4947875639808146 1103 26-27 0.5099507438144641 1103 28-29 0.44095573342372774 1103 30-31 0.41761341055254775 1103 32-33 0.2040057520546199 1103 34-35 0.4895094180665467 1103 36-37 0.46501233522695884 1103 38-39 0.5271340530251578 1103 40-41 0.5181394892039961 1103 42-43 0.5687170734801441 1103 44-45 0.4843370866327277 1103 46-47 0.5747350987428632 1103 48-49 0.5852813810503079 1103 50-51 0.5376995703066285 1103 52-53 0.5105642234662753 1103 54-55 0.5640893374385527 1103 56-57 0.5155540628427033 1103 58-59 0.5941106202440594 1103 60-61 0.5910937597429324 1103 62-63 0.6191920631845775 1103 64-65 0.5154916176151687 1103 66-67 0.5587666071115578 1103 68-69 0.6068290972836934 1103 70-71 0.6300025576953345 1103 72-73 0.6067990622641943 1103 74-75 0.5918536671993451 1103 76-77 0.5601007028369622 1103 78-79 0.6454721130182222 1103 80-81 0.6181509622411809 1103 82-83 0.559929288997921 1103 84-85 0.5004647492099892 1103 86-87 0.500723421960025 1103 88-89 0.516403200879509 1103 90-91 0.5147149119422529 1103 92-93 0.5270867775804007 1103 94-95 0.526823355704348 1103 96-97 0.4937139811970148 1103 98-99 0.6153019161104574 1103 100-101 0.48988364444769417 1103 102-103 0.4764338539225612 1103 104-105 0.6259794801824121 1103 106-107 0.5839546079594733 1103 108-109 0.6000734001986316 1103 110-111 0.4900282850302524 1103 112-113 0.46685462634516384 1103 114-115 0.5513890255286782 1103 116-117 0.587526654721259 1103 118-119 0.6943518164407614 1103 120-121 0.7148596674545225 1103 122-123 0.7233355875982177 1103 124-125 0.7287948317896671 1104 1 0.19746554253882564 1104 2 0.22541196948107967 1104 3 0.3132149387081675 1104 4 0.22186562563463497 1104 5 0.2356148358799004 1104 6 0.19340242440389233 1104 7 0.20505077847390396 1104 8 0.27375541780531876 1104 9 0.37538306204538685 1104 10-11 0.33865485023476793 1104 12-13 0.3384570334219532 1104 14-15 0.4124026539894672 1104 16-17 0.428709899602957 1104 18-19 0.4432779365076911 1104 20-21 0.38544461628913496 1104 22-23 0.32641819164285835 1104 24-25 0.402996910659553 1104 26-27 0.39182558819945257 1104 28-29 0.33175232736497406 1104 30-31 0.3590556131315168 1104 32-33 -0.29414219548348797 1104 34-35 0.313546702088928 1104 36-37 0.3691818359124639 1104 38-39 0.4804971673772158 1104 40-41 0.538468353826147 1104 42-43 0.44606936477435966 1104 44-45 0.5063568453319363 1104 46-47 0.465565787734036 1104 48-49 0.40392850817001147 1104 50-51 0.49867219221530945 1104 52-53 0.47548235393817606 1104 54-55 0.5141343121821649 1104 56-57 0.45239253205043184 1104 58-59 0.5412521059520543 1104 60-61 0.47694787380326886 1104 62-63 0.4814860636312517 1104 64-65 0.4963071578517315 1104 66-67 0.41264200209320023 1104 68-69 0.3879392775246231 1104 70-71 0.3908447167822402 1104 72-73 0.43342539948474723 1104 74-75 0.40674516279189277 1104 76-77 0.42903422169723626 1104 78-79 0.4550650842418662 1104 80-81 0.4416377163977714 1104 82-83 0.3935735629530086 1104 84-85 0.35395908776201424 1104 86-87 0.39795806963694247 1104 88-89 0.36637225820246755 1104 90-91 0.3856868873473367 1104 92-93 0.35292245025937063 1104 94-95 0.4294242169433069 1104 96-97 0.3951565791583391 1104 98-99 0.38528669121803816 1104 100-101 0.3988806502277562 1104 102-103 0.4107151275987455 1104 104-105 0.4162001182333661 1104 106-107 0.42159187643371254 1104 108-109 0.3715033245523678 1104 110-111 0.38362187524101543 1104 112-113 0.37572279891994853 1104 114-115 0.4774559968891019 1104 116-117 0.41608528965831226 1104 118-119 0.44871581586157916 1104 120-121 0.38827807789988356 1104 122-123 0.4644329058906358 1104 124-125 0.5773137975869567 1105 1 0.26076037370845384 1105 2 0.3088017528922222 1105 3 0.32829898445159955 1105 4 0.2796620828831706 1105 5 0.31539990688165176 1105 6 0.3484249815647047 1105 7 0.23786276554127284 1105 8 0.2808922733772121 1105 9 0.24182760964078653 1105 10-11 0.35731355812217913 1105 12-13 0.3748403510451368 1105 14-15 0.5279697404318426 1105 16-17 0.524861413898833 1105 18-19 0.46578116517945745 1105 20-21 0.538304313018287 1105 22-23 0.42075616747281686 1105 24-25 0.5038781586078187 1105 26-27 0.6074337026551362 1105 28-29 0.5208877083297665 1105 30-31 0.5329157613733315 1105 32-33 0.11572557939035022 1105 34-35 0.5300616022705924 1105 36-37 0.5683888717516439 1105 38-39 0.5598034132935439 1105 40-41 0.5858290894830347 1105 42-43 0.6053312524086607 1105 44-45 0.5802970902307223 1105 46-47 0.5496442203121887 1105 48-49 0.5720601720060472 1105 50-51 0.5574099385680995 1105 52-53 0.6194963872471959 1105 54-55 0.6689686511053523 1105 56-57 0.6827666410320177 1105 58-59 0.6237796017799866 1105 60-61 0.6270570311450001 1105 62-63 0.6739536264973296 1105 64-65 0.6623048240247513 1105 66-67 0.6528988220190755 1105 68-69 0.6744999279984043 1105 70-71 0.6996789802334078 1105 72-73 0.6029256203591444 1105 74-75 0.6120903694216153 1105 76-77 0.6934220974607967 1105 78-79 0.7232683211469464 1105 80-81 0.7268075442687945 1105 82-83 0.650674727649843 1105 84-85 0.6269893340963435 1105 86-87 0.5956727189137254 1105 88-89 0.6534423777754768 1105 90-91 0.6076235019365477 1105 92-93 0.5568853116938044 1105 94-95 0.5891382109857624 1105 96-97 0.5827808704866548 1105 98-99 0.5390284011711621 1105 100-101 0.5202553545410602 1105 102-103 0.5184667700387244 1105 104-105 0.580445336051941 1105 106-107 0.6290132892170917 1105 108-109 0.5727650926453336 1105 110-111 0.5508086874824691 1105 112-113 0.5371779600975621 1105 114-115 0.5722876415140696 1105 116-117 0.6135236150345378 1105 118-119 0.6126298540261423 1105 120-121 0.6064133504066405 1105 122-123 0.6454294808860688 1105 124-125 0.7176942887039566 1106 1 0.12215688836052507 1106 2 0.20926784207812332 1106 3 0.28264319234875757 1106 4 0.22283559987270252 1106 5 0.17219402365068248 1106 6 0.26823012326811835 1106 7 0.3017136925272652 1106 8 0.24105149691401095 1106 9 0.2991515147062742 1106 10-11 0.30497666318399297 1106 12-13 0.36129057554208543 1106 14-15 0.43657462529758106 1106 16-17 0.3857234454271534 1106 18-19 0.3681249620240763 1106 20-21 0.3664047425184407 1106 22-23 0.4272062924598359 1106 24-25 0.4745899314230826 1106 26-27 0.43449300955064274 1106 28-29 0.39672837584235054 1106 30-31 0.4136699228656937 1106 32-33 0.33290765219231844 1106 34-35 0.4001142366427928 1106 36-37 0.41668079024434235 1106 38-39 0.4292045446958923 1106 40-41 0.4373670728046548 1106 42-43 0.37125063992761653 1106 44-45 0.4220589568532276 1106 46-47 0.4650446444285947 1106 48-49 0.4207010769135877 1106 50-51 0.37582881769339593 1106 52-53 0.45042962833397837 1106 54-55 0.4634800152665193 1106 56-57 0.3859926061315875 1106 58-59 0.5361947918936991 1106 60-61 0.5323238044999243 1106 62-63 0.5331506528345429 1106 64-65 0.5524116747218457 1106 66-67 0.520749129491989 1106 68-69 0.5268076461158699 1106 70-71 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0.7034427389811455 2203 88-89 0.6495972023454684 2203 90-91 0.653559374821743 2203 92-93 0.6605898055432036 2203 94-95 0.7062982532864872 2203 96-97 0.6759112673520029 2203 98-99 0.605593779989352 2203 100-101 0.6292297188271263 2203 102-103 0.7715091821156044 2203 104-105 0.7392686003783595 2203 106-107 0.7088062168427882 2203 108-109 0.6951535422101465 2203 110-111 0.6179943247669719 2203 112-113 0.7239624454898745 2203 114-115 0.7252409507947277 2203 116-117 0.7599926067961462 2203 118-119 0.8150457961283841 2203 120-121 0.7744172814086063 2203 122-123 0.7503501109401718 2203 124-125 0.8543131158143815 2204 1 0.5224126146395669 2204 2 0.5235206721429577 2204 3 0.4932679090346035 2204 4 0.4988699913908121 2204 5 0.42464539617315467 2204 6 0.4682117142578548 2204 7 0.5313496863984497 2204 8 0.5279854070288152 2204 9 0.6426790297667964 2204 10-11 0.6039665520735653 2204 12-13 0.6604177628299155 2204 14-15 0.741243745131527 2204 16-17 0.7158875061163741 2204 18-19 0.6815661803621182 2204 20-21 0.6700813654342284 2204 22-23 0.6782354535627775 2204 24-25 0.672615500974274 2204 26-27 0.6727189347700673 2204 28-29 0.6862462136092304 2204 30-31 0.7208888700959335 2204 32-33 0.7473613917340529 2204 34-35 0.7568194494181384 2204 36-37 0.7188244400075305 2204 38-39 0.6924574937270336 2204 40-41 0.7532256916058415 2204 42-43 0.7679942962544715 2204 44-45 0.6239281123770581 2204 46-47 0.6469416903721523 2204 48-49 0.5241835591250492 2204 50-51 0.5902273814829258 2204 52-53 0.6335333874641265 2204 54-55 0.6622788658058667 2204 56-57 0.6900122827483557 2204 58-59 0.6745028653984662 2204 60-61 0.623120094186973 2204 62-63 0.6670110786929015 2204 64-65 0.7169873290878002 2204 66-67 0.6598017679800492 2204 68-69 0.6212447149912066 2204 70-71 0.6473053146659709 2204 72-73 0.6181206981296228 2204 74-75 0.6896588383428153 2204 76-77 0.6060597480063237 2204 78-79 0.7335541359096851 2204 80-81 0.7683338487366171 2204 82-83 0.6394809317045542 2204 84-85 0.5908925789244321 2204 86-87 0.5977386150806936 2204 88-89 0.649872489430571 2204 90-91 0.5260847693828232 2204 92-93 0.5658776938337766 2204 94-95 0.5939156954454567 2204 96-97 0.6156946193623263 2204 98-99 0.593320382636719 2204 100-101 0.551842279091808 2204 102-103 0.6167990252134921 2204 104-105 0.5127842534402838 2204 106-107 0.5298243921954082 2204 108-109 0.5153494751348902 2204 110-111 0.5167058428605955 2204 112-113 0.49540614370861746 2204 114-115 0.487208413116651 2204 116-117 0.503698729314145 2204 118-119 0.4755899832282928 2204 120-121 0.45964456170543144 2204 122-123 0.46450741861454503 2204 124-125 0.5171120245553631 2205 1 0.35659764148969586 2205 2 0.33614848400389974 2205 3 0.3788024594915633 2205 4 0.4072792021728162 2205 5 0.4474113296662665 2205 6 0.42184897378648856 2205 7 0.4331119092992566 2205 8 0.4247951612907528 2205 9 0.5158194924958579 2205 10-11 0.5221000670809559 2205 12-13 0.541772884711925 2205 14-15 0.5181405271257375 2205 16-17 0.573122621335969 2205 18-19 0.4713896958938619 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2216 102-103 -2.024457818905514 2216 104-105 -1.8524622157473338 2216 106-107 -1.8456649179721616 2216 108-109 -1.9573715219189793 2216 110-111 -1.863874471218967 2216 112-113 -1.9035538738163105 2216 114-115 -1.9611580066078567 2216 116-117 -2.0571613426027824 2216 118-119 -2.13302396054009 2216 120-121 -2.121852419351967 2216 122-123 -2.2860280220664357 2216 124-125 -2.446073725363423 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 47205.0 3 7885.0 4 4470.0 5 3760.0 6 4253.0 7 4698.0 8 5553.0 9 6337.0 10 6994.0 11 7740.0 12 8358.0 13 8562.0 14 8560.0 15 8044.0 16 6946.0 17 6581.0 18 6364.0 19 6536.0 20 6802.0 21 7380.0 22 8616.0 23 11304.0 24 14854.0 25 18860.0 26 23553.0 27 27847.0 28 33210.0 29 41431.0 30 49889.0 31 61065.0 32 78552.0 33 103311.0 34 142069.0 35 210330.0 36 386342.0 37 807927.0 38 435993.0 39 2054.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.840840177820425 18.893572841360438 12.819213101077878 24.44637387974126 2 15.383722204885853 19.874406590044575 40.8085768053623 23.933294399707272 3 18.914363563657002 24.48756666207071 29.476484802976653 27.121584971295636 4 12.184530538666015 16.147911637996884 38.375332274129974 33.292225549207124 5 13.263137952753137 38.17081028722507 35.28977461316723 13.276277146854557 6 32.11736074380039 36.84636950932044 17.723043809255927 13.313225937623251 7 28.457190681463818 32.09078541074322 21.64958421229631 17.802439695496652 8 25.397585535687057 36.4894681062083 20.09626670325525 18.016679654849398 9 25.975819416170992 16.208872482431392 20.27119069041407 37.544117410983546 10-11 24.52346419415733 26.604750716590885 28.649911424855627 20.221873664396156 12-13 24.866251739773972 24.135838187305104 28.486109430516375 22.511800642404552 14-15 22.134898759612707 26.0307988803604 25.749952507435403 26.084349852591487 16-17 22.255461900945246 29.1024407657841 26.491113004333133 22.15098432893752 18-19 22.069784589838456 28.396638633619244 27.76127938032452 21.772297396217773 20-21 22.77710023487517 27.742830325100183 27.708676046765557 21.77139339325909 22-23 22.503217948487592 27.643716236389903 27.344381081559465 22.50868473356304 24-25 22.266656600282687 27.8349406099273 27.586887069506787 22.31151572028322 26-27 22.30853850734058 28.056507315730467 27.37989901625431 22.25505516067464 28-29 22.05736041637443 28.04258993333713 27.618861605649812 22.28118804463863 30-31 22.34921994002626 27.878022816013637 27.57012703806555 22.20263020589455 32-33 22.157069759939084 27.800232866962325 27.634833105639494 22.407864267459097 34-35 22.46185966435893 27.98989575433618 27.348872073537688 22.199372507767205 36-37 22.538580546124827 27.78194739708464 27.36209676602883 22.317375290761703 38-39 22.163330115655008 28.03210803760769 27.468985686582855 22.33557616015445 40-41 22.467371542305813 27.79570127576902 27.476439166714243 22.26048801521092 42-43 22.288734398317537 27.75528662941017 27.38787815590663 22.568100816365664 44-45 22.27483137050131 27.927378875823717 27.426986105697086 22.370803647977887 46-47 22.294638286818806 27.992680932990044 27.26243591996394 22.45024486022721 48-49 22.300052589462737 27.85936817380464 27.414315757220447 22.426263479512173 50-51 22.23944533265697 27.89687422827849 27.36739090949546 22.496289529569076 52-53 22.312450473723786 27.944880847586173 27.28540905528932 22.457259623400716 54-55 22.34628101793482 27.770879492537436 27.322060206943465 22.560779282584274 56-57 22.21330322088391 27.99264424246428 27.392215544851904 22.401836991799907 58-59 22.279032221410166 27.78816136314461 27.459402642635737 22.473403772809487 60-61 22.434487590725684 27.846815265155705 27.39924763111123 22.31944951300738 62-63 22.10585444274012 27.860386932098276 27.572287864523915 22.46147076063769 64-65 22.17061089077614 27.62870018708047 27.5014969302187 22.699191991924693 66-67 22.123834811786804 27.852909654112374 27.239746697093377 22.783508837007453 68-69 21.992159397814856 27.7783763359149 27.510629819427745 22.718834446842497 70-71 22.153091649489085 27.464283333342888 27.543939174265013 22.83868584290302 72-73 22.129192315907197 27.50092751831252 27.45731218757152 22.912567978208763 74-75 22.034097746874373 27.747485649337627 27.29942024279124 22.91899636099676 76-77 22.04742338107584 27.63018526686325 27.467628584532434 22.854762767528474 78-79 22.073556168755 27.560428207140504 27.449108564066393 22.916907060038103 80-81 22.002755779237365 27.683049856375114 27.394061721737362 22.920132642650163 82-83 22.153015427394887 27.647145574715253 27.429101188718235 22.770737809171617 84-85 22.168782040787946 27.45112684431823 27.401288537055613 22.97880257783821 86-87 21.937670103971023 27.34577542388826 27.6535264442972 23.063028027843522 88-89 22.148971837367466 27.310414726262632 27.54463852517323 22.99597491119667 90-91 22.151221818942986 27.428167955318255 27.566232311757684 22.854377913981075 92-93 21.990327243822172 27.26309281823733 27.64164926929113 23.104930668649374 94-95 21.93142776386828 27.21169620127458 27.633373629500614 23.223502405356523 96-97 21.960508363400965 27.110251384193223 27.671318676177552 23.257921576228256 98-99 21.899413251603164 27.0208154615399 27.58610816190728 23.493663124949656 100-101 21.847868330252478 27.05178510209582 27.624492155079672 23.47585441257203 102-103 21.875526664718585 26.877703466028823 27.68093456063828 23.56583530861431 104-105 21.789471594667962 26.90473709144395 27.663413831554216 23.64237748233387 106-107 21.748991820037777 26.884048233833617 27.723021165289595 23.643938780839008 108-109 21.674401965891636 26.923308197190092 27.553369365008066 23.848920471910205 110-111 21.739052400426857 26.928955521853382 27.564101217765142 23.767890859954623 112-113 21.6783948478105 26.9573269866546 27.718909341207493 23.64536882432741 114-115 21.51453616246533 27.001204346338543 27.590012860616575 23.894246630579552 116-117 21.446056064946085 26.97446844905412 27.73358421463792 23.845891271361882 118-119 21.45743173027761 26.968877612703128 27.56849795819675 24.005192698822515 120-121 21.46316449858055 27.01524105813679 27.604467756582316 23.917126686700335 122-123 21.517665941137647 27.10497121334276 27.518448298277765 23.858914547241827 124-125 21.415410887370697 27.013806921522754 27.50570021907323 24.065081972033315 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 10953.0 1 5796.0 2 680.0 3 656.5 4 537.0 5 442.0 6 361.0 7 318.0 8 338.5 9 352.5 10 331.5 11 332.0 12 324.5 13 327.5 14 380.5 15 435.5 16 475.0 17 533.5 18 702.5 19 941.5 20 1289.0 21 1844.5 22 2524.0 23 3688.5 24 5433.5 25 7874.5 26 11641.5 27 15991.0 28 19924.0 29 24779.5 30 30867.5 31 37246.0 32 44297.0 33 51596.0 34 58992.0 35 65821.5 36 74067.0 37 81976.5 38 84634.0 39 86555.5 40 89323.5 41 91704.5 42 94043.0 43 96324.5 44 99082.0 45 101349.5 46 101510.5 47 100639.5 48 98981.5 49 97236.5 50 95397.0 51 92075.0 52 89367.0 53 86332.5 54 82955.0 55 76143.5 56 66237.5 57 58958.0 58 53523.0 59 46765.0 60 39398.0 61 32708.0 62 26405.0 63 20883.5 64 16108.5 65 11877.0 66 8516.5 67 6886.5 68 5301.0 69 3364.0 70 2418.5 71 1947.5 72 1515.0 73 1246.5 74 952.0 75 678.5 76 492.5 77 255.5 78 145.5 79 107.0 80 64.0 81 42.5 82 31.0 83 24.0 84 19.5 85 13.5 86 16.5 87 12.0 88 9.5 89 11.5 90 10.5 91 8.5 92 5.0 93 5.5 94 5.5 95 4.0 96 2.5 97 0.5 98 1.5 99 2.5 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.46813307556168937 2 0.46018701750984226 3 0.45592884286004864 4 0.4530013477883155 5 0.4605672116750025 6 0.5177864335316046 7 0.513832414213939 8 0.5229190547612667 9 0.529268297319441 10-11 0.5466051512507438 12-13 0.5548363549264609 14-15 0.5336405302187828 16-17 0.5278615789083485 18-19 0.5382028602007045 20-21 0.5385260252410906 22-23 0.5489243356582207 24-25 0.5424420251422402 26-27 0.5500459084454431 28-29 0.5298385885671812 30-31 0.5356935787106476 32-33 0.5379367242850924 34-35 0.5363399087914198 36-37 0.4849946867865419 38-39 0.4519558138341251 40-41 0.4506631536725806 42-43 0.46262026016686725 44-45 0.46731565810659503 46-47 0.4720110560463228 48-49 0.4862873469480864 50-51 0.46784792993781926 52-53 0.46788594935433525 54-55 0.45744961952068924 56-57 0.44125334808486694 58-59 0.4582670369757835 60-61 0.4551494448214703 62-63 0.4708894832591004 64-65 0.47058532792697233 66-67 0.47822723064669126 68-69 0.48794119156653304 70-71 0.4837780654580294 72-73 0.495621113702768 74-75 0.48512775474434794 76-77 0.48233332763042086 78-79 0.49413835645864346 80-81 0.4876560459426629 82-83 0.48961404589323765 84-85 0.48552695861776607 86-87 0.4765543763199866 88-89 0.4810596771771343 90-91 0.4658519105707285 92-93 0.4662511144441466 94-95 0.4607953281740985 96-97 0.44986474592574427 98-99 0.4138033293603043 100-101 0.4118643391179876 102-103 0.4016941451999536 104-105 0.40581925189194124 106-107 0.4054010383102651 108-109 0.4169019118063595 110-111 0.43891515396913205 112-113 0.4388391151361 114-115 0.4329270958678597 116-117 0.42585548439588095 118-119 0.46872237651768756 120-121 0.4616507650457088 122-123 0.4268249795170394 124-125 0.43631082393778503 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2630235.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.54771144776826 #Duplication Level Percentage of deduplicated Percentage of total 1 83.78409673287528 54.080716998255376 2 9.743635906137655 12.578587978429749 3 2.9584768995289457 5.728887397070474 4 1.2072385655032964 3.1169794629889784 5 0.6273324116446558 2.024643574433591 6 0.37377019447471804 1.4475606396438194 7 0.22679182959284214 1.0247225502689143 8 0.16136766352618978 0.8332730705831246 9 0.11839030911109422 0.6877641159643499 >10 0.672967165895049 8.38161530391536 >50 0.07822014508874896 3.490083889310125 >100 0.04726626860601175 5.472100158823942 >500 2.972720103831376E-4 0.09997066760756251 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 1.486360051915688E-4 1.0330941927046384 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 10768 0.4093930770444466 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 3.801941651601473E-5 0.0 16-17 0.0 0.0 0.0 7.603883303202946E-5 0.0 18-19 0.0 0.0 0.0 1.3306795780605154E-4 0.0 20-21 0.0 0.0 0.0 2.09106790838081E-4 3.801941651601473E-5 22-23 0.0 0.0 0.0 3.421747486441326E-4 3.801941651601473E-5 24-25 0.0 0.0 0.0 5.322718312242062E-4 3.801941651601473E-5 26-27 0.0 0.0 0.0 9.504854129003682E-4 3.801941651601473E-5 28-29 0.0 0.0 0.0 0.0024142329487669353 3.801941651601473E-5 30-31 0.0 0.0 0.0 0.007109630888494754 3.801941651601473E-5 32-33 0.0 0.0 0.0 0.017621999555172827 3.801941651601473E-5 34-35 0.0 0.0 0.0 0.035795280649827864 3.801941651601473E-5 36-37 0.0 0.0 0.0 0.06674308569386386 3.801941651601473E-5 38-39 0.0 0.0 0.0 0.11154896805798721 3.801941651601473E-5 40-41 0.0 0.0 0.0 0.15884512220390956 3.801941651601473E-5 42-43 0.0 0.0 0.0 0.20804224717563258 3.801941651601473E-5 44-45 0.0 0.0 0.0 0.2704130999701547 3.801941651601473E-5 46-47 0.0 0.0 0.0 0.34780162228850275 3.801941651601473E-5 48-49 0.0 0.0 0.0 0.43279402791005367 3.801941651601473E-5 50-51 0.0 0.0 0.0 0.5233372683429427 3.801941651601473E-5 52-53 0.0 0.0 0.0 0.6128920039464154 3.801941651601473E-5 54-55 0.0 0.0 0.0 0.7153163120405591 3.801941651601473E-5 56-57 0.0 0.0 0.0 0.8398869302552814 3.801941651601473E-5 58-59 0.0 0.0 0.0 0.980957975237954 3.801941651601473E-5 60-61 0.0 0.0 0.0 1.1352407674599418 3.801941651601473E-5 62-63 0.0 0.0 0.0 1.2872994238157427 3.801941651601473E-5 64-65 0.0 0.0 0.0 1.4499845070877697 3.801941651601473E-5 66-67 0.0 1.9009708258007364E-5 0.0 1.6446059002332492 3.801941651601473E-5 68-69 0.0 3.801941651601473E-5 0.0 1.8646242636114263 3.801941651601473E-5 70-71 0.0 3.801941651601473E-5 0.0 2.109469305974561 3.801941651601473E-5 72-73 0.0 3.801941651601473E-5 0.0 2.359446969567358 3.801941651601473E-5 74-75 0.0 3.801941651601473E-5 0.0 2.6162491184247796 3.801941651601473E-5 76-77 0.0 3.801941651601473E-5 0.0 2.899303674386509 3.801941651601473E-5 78-79 0.0 3.801941651601473E-5 0.0 3.2282286563748106 3.801941651601473E-5 80-81 0.0 3.801941651601473E-5 0.0 3.5919414044752656 3.801941651601473E-5 82-83 0.0 3.801941651601473E-5 0.0 3.9686758027324553 3.801941651601473E-5 84-85 0.0 3.801941651601473E-5 0.0 4.348755909643055 3.801941651601473E-5 86-87 0.0 3.801941651601473E-5 0.0 4.744290909367415 3.801941651601473E-5 88-89 0.0 3.801941651601473E-5 0.0 5.185962471033957 3.801941651601473E-5 90-91 0.0 3.801941651601473E-5 0.0 5.668409856913926 3.801941651601473E-5 92-93 0.0 3.801941651601473E-5 0.0 6.1805313973846445 3.801941651601473E-5 94-95 0.0 3.801941651601473E-5 0.0 6.698184762958443 3.801941651601473E-5 96-97 0.0 5.7029124774022093E-5 0.0 7.217054749860754 3.801941651601473E-5 98-99 0.0 7.603883303202946E-5 0.0 7.774305337735981 3.801941651601473E-5 100-101 0.0 7.603883303202946E-5 0.0 8.398850292844555 3.801941651601473E-5 102-103 0.0 7.603883303202946E-5 0.0 9.047518567732542 3.801941651601473E-5 104-105 0.0 1.1405824954804419E-4 0.0 9.712516182014154 3.801941651601473E-5 106-107 0.0 1.1405824954804419E-4 0.0 10.353105330892486 3.801941651601473E-5 108-109 0.0 1.1405824954804419E-4 0.0 11.031067566206062 3.801941651601473E-5 110-111 0.0 1.1405824954804419E-4 0.0 11.760451062357546 3.801941651601473E-5 112-113 0.0 1.1405824954804419E-4 0.0 12.549487023022657 3.801941651601473E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2560 0.0 52.77029 1 GTATCAA 4170 0.0 46.810295 1 TCAACGC 4525 0.0 41.177708 4 ATCAACG 4510 0.0 41.04282 3 CAACGCA 4685 0.0 39.904594 5 AACGCAG 4965 0.0 37.534985 6 TATCAAC 5305 0.0 35.226765 2 GTACATG 8975 0.0 33.154243 1 TACATGG 8955 0.0 32.166832 2 ACATGGG 9095 0.0 31.346292 3 CGCAGAG 5920 0.0 30.87825 8 ACGCAGA 6025 0.0 30.735437 7 GAGTACA 6650 0.0 25.415594 1 GCAGAGT 7115 0.0 24.269405 9 AGTACAT 6745 0.0 23.647285 2 CATGGGG 4480 0.0 23.519693 4 TAGACCG 185 2.6785277E-5 22.528337 5 CAGAGTA 6815 0.0 20.794237 10-11 AGAGTAC 6125 0.0 20.657831 10-11 CATGGGA 5970 0.0 20.441648 4 >>END_MODULE