FastQCFastQC Report
Thu 26 May 2016
SRR1364105_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364105_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2630235
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52430.19933580079346522No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52040.19785304354934066No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27990.10641634682832521No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42150.072.1110461
GTATCAA69650.048.003281
ATCAACG86450.038.057253
TCAACGC87900.037.4971434
CAACGCA89200.036.8839655
TATCAAC92700.035.427862
AACGCAG94400.034.9789286
GTACATG87200.031.7125171
TACATGG88950.030.0990052
ACATGGG87400.029.611143
TGGTATC20700.029.6041372
CGCAGAG112600.029.1171118
ACGCAGA114200.028.657067
GTGGTAT23950.027.3726231
GCAGAGT125850.025.767869
GAGTACT85350.025.23689812-13
CAGAGTA124400.024.4180210-11
GAGTACA66950.023.1447221
GTACTTT92600.023.12963514-15
AGAGTAC117700.023.00223210-11
Produced by FastQC (version 0.11.5)