FastQCFastQC Report
Thu 26 May 2016
SRR1364104_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364104_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1934287
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN78060.40355955450251174No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25800.057.4326671
GTATCAA45350.048.0267451
TCAACGC51550.040.8801084
ATCAACG51950.040.784983
CAACGCA53750.039.208915
AACGCAG55850.037.843196
TATCAAC60000.035.7079162
GTACATG112900.035.631341
TACATGG113450.034.831872
ACATGGG114450.032.865263
ACGCAGA66650.031.5340467
CGCAGAG67250.031.0756328
CATGGGA76000.029.5301174
GAGTACA83050.025.7953991
CATGGGG39250.025.7837854
GCAGAGT77500.025.4291279
AGTACAT81450.025.1350982
ATGGGAG31400.024.4579475
GTATAGG7850.021.9840491
ATGGGAT34100.021.823065
Produced by FastQC (version 0.11.5)