##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364104_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1934287 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.088013826283277 31.0 31.0 34.0 28.0 34.0 2 31.089969068705937 33.0 31.0 34.0 28.0 34.0 3 31.15883837300256 33.0 31.0 34.0 27.0 34.0 4 34.606769833018575 37.0 35.0 37.0 32.0 37.0 5 34.65164114735818 37.0 35.0 37.0 32.0 37.0 6 34.688101610567614 37.0 35.0 37.0 32.0 37.0 7 34.56946202916113 37.0 35.0 37.0 31.0 37.0 8 34.529041450415576 37.0 35.0 37.0 31.0 37.0 9 36.00927163342358 39.0 37.0 39.0 30.0 39.0 10-11 35.93902223403249 39.0 37.0 39.0 30.0 39.0 12-13 35.84660756133914 39.0 37.0 39.0 30.0 39.0 14-15 37.108489071166794 40.0 37.0 41.0 30.5 41.0 16-17 37.14289270413336 40.0 37.0 41.0 31.0 41.0 18-19 37.16810871396024 40.0 37.5 41.0 31.0 41.0 20-21 37.2042662231613 40.0 37.5 41.0 31.0 41.0 22-23 37.17895948222782 40.0 37.0 41.0 31.0 41.0 24-25 37.211765368841334 40.0 37.0 41.0 31.0 41.0 26-27 37.197839824183276 40.0 37.0 41.0 31.0 41.0 28-29 37.16926960683704 40.0 37.0 41.0 31.0 41.0 30-31 37.1340416391156 40.0 37.0 41.0 31.0 41.0 32-33 37.050843799291414 40.0 37.0 41.0 30.0 41.0 34-35 37.02838927211939 40.0 37.0 41.0 30.0 41.0 36-37 36.97206153998863 40.0 37.0 41.0 30.0 41.0 38-39 36.891862221066475 40.0 37.0 41.0 30.0 41.0 40-41 36.82217969722177 40.0 37.0 41.0 30.0 41.0 42-43 36.716255653892105 40.0 36.5 41.0 30.0 41.0 44-45 36.6260097389891 40.0 36.0 41.0 30.0 41.0 46-47 36.519910695775756 40.0 36.0 41.0 30.0 41.0 48-49 36.41973838422116 40.0 36.0 41.0 30.0 41.0 50-51 36.30708188598693 40.0 36.0 41.0 29.5 41.0 52-53 36.176047815034686 40.0 35.0 41.0 29.0 41.0 54-55 36.004734302613834 39.0 35.0 41.0 29.0 41.0 56-57 35.835769717730614 39.0 35.0 41.0 28.0 41.0 58-59 35.637650203925276 39.0 35.0 41.0 28.0 41.0 60-61 35.408099470244075 39.0 35.0 41.0 28.0 41.0 62-63 35.15804195551125 38.0 34.5 40.5 28.0 41.0 64-65 34.88829346420671 38.0 34.0 40.0 27.5 41.0 66-67 34.58646648610056 37.0 34.0 40.0 26.5 41.0 68-69 34.26415340639729 37.0 34.0 40.0 26.0 41.0 70-71 33.92634521143967 36.0 34.0 39.0 26.0 41.0 72-73 33.567535737974765 36.0 33.0 39.0 26.0 40.5 74-75 33.28444977399941 35.0 33.0 38.5 26.0 40.0 76-77 33.04415916562537 35.0 33.0 37.0 26.0 39.5 78-79 32.72295372920358 35.0 33.0 37.0 26.0 39.0 80-81 32.486485201006886 35.0 33.0 36.5 26.0 39.0 82-83 32.354855303271954 35.0 33.0 36.0 26.0 38.0 84-85 32.10888947710448 35.0 33.0 36.0 26.0 37.0 86-87 31.889167429652375 35.0 33.0 35.5 25.0 37.0 88-89 31.687418930076042 35.0 33.0 35.0 25.0 36.0 90-91 31.52130190607702 35.0 33.0 35.0 25.0 36.0 92-93 31.38676706197167 35.0 33.0 35.0 24.0 36.0 94-95 31.261377706617477 35.0 33.0 35.0 24.0 36.0 96-97 31.15944195458068 35.0 33.0 35.0 23.5 35.5 98-99 31.06541376745023 35.0 33.0 35.0 23.0 35.0 100-101 30.97976928966591 35.0 33.0 35.0 21.5 35.0 102-103 29.896635556150663 34.0 30.0 35.0 19.0 35.0 104-105 30.68206889670457 35.0 32.0 35.0 19.0 35.0 106-107 30.756062569825474 35.0 32.5 35.0 19.0 35.0 108-109 30.714370204628374 35.0 33.0 35.0 18.5 35.0 110-111 30.628176945820343 35.0 32.0 35.0 17.5 35.0 112-113 30.525075389536298 35.0 32.0 35.0 12.5 35.0 114-115 30.39967130007078 35.0 32.0 35.0 6.0 35.0 116-117 30.282770860787462 35.0 32.0 35.0 2.0 35.0 118-119 30.13416080447214 35.0 32.0 35.0 2.0 35.0 120-121 29.967419519440497 35.0 32.0 35.0 2.0 35.0 122-123 29.771897345119932 35.0 32.0 35.0 2.0 35.0 124-125 28.915537353040165 34.5 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.41898344367716334 1101 2 0.4356554777482877 1101 3 0.43656469773685913 1101 4 0.5216923217038101 1101 5 0.45793355882881315 1101 6 0.3858216004343049 1101 7 0.47291165411463254 1101 8 0.4465839916683194 1101 9 0.4568431671097315 1101 10-11 0.6064246825234321 1101 12-13 0.5810137218298053 1101 14-15 0.7288753711242109 1101 16-17 0.6990913372280616 1101 18-19 0.6141873061845757 1101 20-21 0.6092236337452164 1101 22-23 0.674187296179035 1101 24-25 0.6385569473987616 1101 26-27 0.6569448959382385 1101 28-29 0.6089723515592951 1101 30-31 0.5819405690178456 1101 32-33 0.5654988835390284 1101 34-35 0.6010046710878711 1101 36-37 0.6217781079456586 1101 38-39 0.6373588110059387 1101 40-41 0.6822755127011959 1101 42-43 0.6189918506263226 1101 44-45 0.699452243563158 1101 46-47 0.6995450011408835 1101 48-49 0.6186287876152434 1101 50-51 0.7123410346870642 1101 52-53 0.7613703176230828 1101 54-55 0.706474158552389 1101 56-57 0.7808647054103162 1101 58-59 0.7260120502547736 1101 60-61 0.7740719609317068 1101 62-63 0.778619393694612 1101 64-65 0.7823661084416216 1101 66-67 0.7864096538306242 1101 68-69 0.840344845107424 1101 70-71 0.7943822315401903 1101 72-73 0.7616445418931619 1101 74-75 0.6566951275946096 1101 76-77 0.6013686648350998 1101 78-79 0.635210484687569 1101 80-81 0.6398894223652505 1101 82-83 0.6893545762082383 1101 84-85 0.6374703587586197 1101 86-87 0.5948636952321742 1101 88-89 0.6502636420814341 1101 90-91 0.6455623084504793 1101 92-93 0.7186155070844862 1101 94-95 0.7156093115184312 1101 96-97 0.6720818325856364 1101 98-99 0.6134542172120021 1101 100-101 0.7493650665039304 1101 102-103 0.7061707543074789 1101 104-105 0.6394978008252572 1101 106-107 0.6867183534878087 1101 108-109 0.7461318531127894 1101 110-111 0.700400064612964 1101 112-113 0.5791553773276554 1101 114-115 0.573026784997591 1101 116-117 0.5857149809556716 1101 118-119 0.6761866888131927 1101 120-121 0.7218613061664456 1101 122-123 0.8271772719926318 1101 124-125 0.8512589510653896 1102 1 0.41371454832832555 1102 2 0.45663195766372056 1102 3 0.42140217130979707 1102 4 0.49942752254948175 1102 5 0.3611196714080904 1102 6 0.5220035507514282 1102 7 0.560203541006814 1102 8 0.4102054900826957 1102 9 0.5524348029448234 1102 10-11 0.46643558368622706 1102 12-13 0.5549748079820276 1102 14-15 0.5889647276252674 1102 16-17 0.6234273573126288 1102 18-19 0.5892178624742144 1102 20-21 0.5907742415676225 1102 22-23 0.6103576846568401 1102 24-25 0.6900484246714882 1102 26-27 0.6566971422384427 1102 28-29 0.6903511834831875 1102 30-31 0.7283960394195361 1102 32-33 0.6986128172071062 1102 34-35 0.7458827762675782 1102 36-37 0.734055955461514 1102 38-39 0.770328221682476 1102 40-41 0.7914275343713797 1102 42-43 0.774469684928647 1102 44-45 0.8114947250642146 1102 46-47 0.7565077059506038 1102 48-49 0.8124960576998106 1102 50-51 0.8873996697293407 1102 52-53 0.8348086434687403 1102 54-55 0.7575320668504801 1102 56-57 0.851424959110112 1102 58-59 0.8688544458678749 1102 60-61 0.8940590777393211 1102 62-63 0.7982001944345711 1102 64-65 0.7090062379342186 1102 66-67 0.7389235280378088 1102 68-69 0.7120951424118687 1102 70-71 0.8087065246162979 1102 72-73 0.7600424013011917 1102 74-75 0.7241832354170157 1102 76-77 0.7249895025729458 1102 78-79 0.6849676860617748 1102 80-81 0.6444976411813244 1102 82-83 0.6633941176987292 1102 84-85 0.6670025997734257 1102 86-87 0.6331664172194813 1102 88-89 0.6535133447769894 1102 90-91 0.69274287134688 1102 92-93 0.6682017584058357 1102 94-95 0.7665928937065942 1102 96-97 0.7591879042029746 1102 98-99 0.7809150721274349 1102 100-101 0.800026568882366 1102 102-103 0.7946023082186819 1102 104-105 0.8639213605504139 1102 106-107 0.8180608482023963 1102 108-109 0.7673602159563409 1102 110-111 0.8417807133973199 1102 112-113 0.8551034481521782 1102 114-115 0.7732654543950552 1102 116-117 0.8410582024144446 1102 118-119 0.7260429916673168 1102 120-121 0.7645823024666569 1102 122-123 0.8256494575106004 1102 124-125 1.0177287911816677 1103 1 0.241981691308073 1103 2 0.3146957313428409 1103 3 0.34821974847173465 1103 4 0.26255051353034986 1103 5 0.36346429913489686 1103 6 0.28322455650931744 1103 7 0.38417177314799744 1103 8 0.3494057200471161 1103 9 0.4887687672662082 1103 10-11 0.4641063161785013 1103 12-13 0.45725857998370856 1103 14-15 0.5083760582005823 1103 16-17 0.4509701318655672 1103 18-19 0.41488382549655967 1103 20-21 0.47449884600541026 1103 22-23 0.5107909159788093 1103 24-25 0.5349320332209331 1103 26-27 0.5008808855677245 1103 28-29 0.5598375962722528 1103 30-31 0.548750962881762 1103 32-33 0.2864033569700837 1103 34-35 0.4768374455791289 1103 36-37 0.5098959278228747 1103 38-39 0.6157538265337692 1103 40-41 0.6392486019602472 1103 42-43 0.6069628996128245 1103 44-45 0.5925814922598605 1103 46-47 0.6855641569500435 1103 48-49 0.6331422157920557 1103 50-51 0.581684193162701 1103 52-53 0.6228757159377736 1103 54-55 0.6260503591647151 1103 56-57 0.5394003944626036 1103 58-59 0.5761601683540718 1103 60-61 0.6547859964501157 1103 62-63 0.6283955325349311 1103 64-65 0.5712859041249629 1103 66-67 0.5796019718476941 1103 68-69 0.5777325403029749 1103 70-71 0.6385682655776606 1103 72-73 0.6781027030831694 1103 74-75 0.6772602042557736 1103 76-77 0.668179732303301 1103 78-79 0.6642732042326642 1103 80-81 0.6143291956549959 1103 82-83 0.535449405222522 1103 84-85 0.5240628391392761 1103 86-87 0.48952520658064813 1103 88-89 0.48437386569809604 1103 90-91 0.502142910942112 1103 92-93 0.5482483827660758 1103 94-95 0.5489197547799947 1103 96-97 0.5887903500374776 1103 98-99 0.6098402054443817 1103 100-101 0.6369927768880146 1103 102-103 0.5988702060434825 1103 104-105 0.6615903375890824 1103 106-107 0.6778600828246262 1103 108-109 0.7552152198768525 1103 110-111 0.6519834463460157 1103 112-113 0.6468890955634699 1103 114-115 0.8355375501921039 1103 116-117 0.7548623825395921 1103 118-119 0.853209148277184 1103 120-121 0.828295150241523 1103 122-123 0.8987922607359522 1103 124-125 1.0609757927693764 1104 1 0.42927957798638516 1104 2 0.3852583295472449 1104 3 0.38041318602780905 1104 4 0.4402224219975537 1104 5 0.24837765886289276 1104 6 0.38728092101724343 1104 7 0.39583771993848416 1104 8 0.47197667696018897 1104 9 0.5991648333165855 1104 10-11 0.5294126204971192 1104 12-13 0.5830847906154091 1104 14-15 0.5494731423067947 1104 16-17 0.6269690768457039 1104 18-19 0.6017582309399216 1104 20-21 0.62791422126692 1104 22-23 0.5976333162783547 1104 24-25 0.606641821788152 1104 26-27 0.6200602195397238 1104 28-29 0.6643934422537257 1104 30-31 0.7028501606210824 1104 32-33 0.07875106123584175 1104 34-35 0.6028983645915034 1104 36-37 0.6710567395365672 1104 38-39 0.8013306581634794 1104 40-41 0.7886924407185205 1104 42-43 0.8165184353647987 1104 44-45 0.7909851159320667 1104 46-47 0.7510889394959364 1104 48-49 0.7632666022503329 1104 50-51 0.7804347588224729 1104 52-53 0.7939149416432727 1104 54-55 0.8347606093630091 1104 56-57 0.8219364020460986 1104 58-59 0.8079361016837154 1104 60-61 0.7447341653654078 1104 62-63 0.7817918628193965 1104 64-65 0.795367247497758 1104 66-67 0.7823470118289677 1104 68-69 0.8400873403199398 1104 70-71 0.8201609838325012 1104 72-73 0.8082506262583706 1104 74-75 0.7132324527297129 1104 76-77 0.7198660033183515 1104 78-79 0.6863502254736815 1104 80-81 0.6864828280112434 1104 82-83 0.6601112371001747 1104 84-85 0.5762382306812412 1104 86-87 0.5764587592546313 1104 88-89 0.5359614382157432 1104 90-91 0.5753484488860536 1104 92-93 0.5690002808555654 1104 94-95 0.6549027163529786 1104 96-97 0.6598868064979051 1104 98-99 0.6774695777339197 1104 100-101 0.6596197667587234 1104 102-103 0.5768237286335811 1104 104-105 0.6231550585298606 1104 106-107 0.700010469150186 1104 108-109 0.6968951880825216 1104 110-111 0.6867081889333875 1104 112-113 0.6167565281496437 1104 114-115 0.5713008309493226 1104 116-117 0.6565634104173625 1104 118-119 0.7010638573813317 1104 120-121 0.6962510540526523 1104 122-123 0.841556714615848 1104 124-125 0.7956599779402183 1105 1 0.21178852345095933 1105 2 0.1472615365641481 1105 3 0.07402986290691871 1105 4 0.10653397601192438 1105 5 0.11415045555474279 1105 6 0.037263731836986835 1105 7 0.003225595380257573 1105 8 0.07254169485111817 1105 9 0.14236664700738544 1105 10-11 0.21044892023142125 1105 12-13 0.16393347643778355 1105 14-15 0.20783683993175828 1105 16-17 0.21311706712978662 1105 18-19 0.3581649293898721 1105 20-21 0.30523002929776766 1105 22-23 0.31485112886592503 1105 24-25 0.31159259911564874 1105 26-27 0.21536529937037585 1105 28-29 0.3080876538278332 1105 30-31 0.19187781539583426 1105 32-33 -0.13579435952252084 1105 34-35 0.23138980985680035 1105 36-37 0.24491006472602095 1105 38-39 0.28291696276357214 1105 40-41 0.38854825210407284 1105 42-43 0.29939221690131745 1105 44-45 0.3572854713055378 1105 46-47 0.32468467149338665 1105 48-49 0.309276145057261 1105 50-51 0.38362717836952953 1105 52-53 0.41696245371114316 1105 54-55 0.3369350528908157 1105 56-57 0.439858457549299 1105 58-59 0.3929154477711094 1105 60-61 0.354201491496724 1105 62-63 0.42140272716375904 1105 64-65 0.4746713033129524 1105 66-67 0.40024913961963904 1105 68-69 0.44654766020985903 1105 70-71 0.36853471133191107 1105 72-73 0.39216756853059564 1105 74-75 0.4333449183819198 1105 76-77 0.3792782898412028 1105 78-79 0.31605787248274453 1105 80-81 0.3498832244137873 1105 82-83 0.2496062272976971 1105 84-85 0.22810304890383293 1105 86-87 0.23634148650023334 1105 88-89 0.22990197098741305 1105 90-91 0.2753624105104109 1105 92-93 0.25754419346508683 1105 94-95 0.30369903361389916 1105 96-97 0.256632305402249 1105 98-99 0.2744186327202236 1105 100-101 0.26136907760218264 1105 102-103 0.3213729491054771 1105 104-105 0.4032319672746283 1105 106-107 0.4137887341041626 1105 108-109 0.3343384552297657 1105 110-111 0.26401169802017677 1105 112-113 0.2747258282248026 1105 114-115 0.3641584942673184 1105 116-117 0.4204137819041591 1105 118-119 0.45515001649690134 1105 120-121 0.4601827257163258 1105 122-123 0.37667557914212324 1105 124-125 0.42787546033023105 1106 1 0.23020760388387984 1106 2 0.2127872606940251 1106 3 0.2829552016128254 1106 4 0.29759671446866776 1106 5 0.16096973453940677 1106 6 0.17817463661001653 1106 7 0.09153245514822572 1106 8 0.07405492190087415 1106 9 0.1430834771872469 1106 10-11 0.10367516701955992 1106 12-13 0.17453762363859227 1106 14-15 0.29294594605961066 1106 16-17 0.21883455428232423 1106 18-19 0.17664289403987254 1106 20-21 0.24102676682014845 1106 22-23 0.31290661142451626 1106 24-25 0.29775833628765014 1106 26-27 0.2967381775919762 1106 28-29 0.3436340117660208 1106 30-31 0.2702521000255942 1106 32-33 0.28119252048993104 1106 34-35 0.36609268788374294 1106 36-37 0.31321867642110846 1106 38-39 0.3286378807733854 1106 40-41 0.46083252174511813 1106 42-43 0.3426554488175384 1106 44-45 0.38452976294299646 1106 46-47 0.3750012349910108 1106 48-49 0.3314275602250589 1106 50-51 0.27863625432530625 1106 52-53 0.20585109927682055 1106 54-55 0.3571887903353357 1106 56-57 0.33777281264546133 1106 58-59 0.29041773500929224 1106 60-61 0.28597037824437166 1106 62-63 0.30475795958608387 1106 64-65 0.2993234727827101 1106 66-67 0.2765340078357923 1106 68-69 0.2909343153916666 1106 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-2.2350179559732446 2216 122-123 -2.316132752592843 2216 124-125 -2.6913263452109177 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 34936.0 3 6120.0 4 3449.0 5 3038.0 6 3657.0 7 4190.0 8 4690.0 9 5102.0 10 5777.0 11 6404.0 12 6727.0 13 6782.0 14 7000.0 15 6464.0 16 5809.0 17 5477.0 18 5282.0 19 5212.0 20 5372.0 21 5786.0 22 6962.0 23 9173.0 24 11850.0 25 14843.0 26 18094.0 27 20970.0 28 25341.0 29 31489.0 30 37601.0 31 44589.0 32 57968.0 33 76645.0 34 105592.0 35 155517.0 36 283406.0 37 581056.0 38 314271.0 39 1646.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.570968537779336 18.994247724410165 12.857309804838144 23.577473932972357 2 15.378203456975806 20.36844494255512 40.80648227108 23.446869329389074 3 19.32268630099496 25.54867114629309 29.33449037108818 25.79415218162377 4 12.403247307282657 16.463264226183583 38.05267500910079 33.08081345743297 5 13.220992300205763 38.890296888194584 34.466057233786856 13.422653577812794 6 31.464064697937893 36.540273302331286 18.319944668151813 13.67571733157901 7 28.21553671391626 32.927141677591806 21.224748124279994 17.632573484211946 8 25.82015261843935 35.89188868956428 20.325150861934567 17.9628078300618 9 26.727013729840355 16.57558999752105 20.200311505594783 36.49708476704381 10-11 24.53347631197239 27.207880320017797 28.16116076754163 20.09748260046818 12-13 25.00931817957341 24.85232569805771 28.049332028181713 22.08902409418717 14-15 22.349206464701695 26.528477643465354 25.72092265418347 25.401393237649483 16-17 22.159070830033283 29.74555298480625 26.30583324498548 21.789542940174993 18-19 21.897906582334667 28.917251347854943 27.882444907257497 21.302397162552893 20-21 23.099684429617515 28.02981221444233 27.467591210183702 21.40291214575646 22-23 22.577498653050498 28.231593704411317 27.131656419107784 22.059251223430397 24-25 22.19111628696317 28.239976110918935 27.548461022879927 22.020446579237966 26-27 22.14353122866331 28.47898357885391 27.380437209343427 21.997047983139353 28-29 21.904318170951505 28.46911983901446 27.58031087005781 22.04625111997622 30-31 22.188759638250673 28.262127674613748 27.582541834510156 21.966570852625427 32-33 21.954191577707636 28.36048924825352 27.466134584329733 22.219184589709112 34-35 22.353843383234395 28.451791945127976 27.284119019324176 21.91024565231345 36-37 22.388165532932824 28.17410411650576 27.239299656373454 22.19843069418796 38-39 22.05768336981386 28.434063722779168 27.40063925304502 22.107613654361955 40-41 22.216585038574173 28.23186099847562 27.57007756378117 21.981476399169033 42-43 22.125004674198014 28.108809097443526 27.506616586962934 22.25956964139553 44-45 22.14469926578111 28.319559858199383 27.401533048419807 22.134207827599703 46-47 22.073978657705222 28.364787550934018 27.21682763850547 22.344406152855292 48-49 21.998734031564016 28.34668002764663 27.420320481701772 22.23426545908758 50-51 22.05938110275481 28.32018427722608 27.354546682178913 22.265887937840194 52-53 22.178184704981383 28.376472470218197 27.32483509086655 22.120507733933874 54-55 22.10494609360394 28.184086707243605 27.373647146804046 22.33732005234841 56-57 22.01806137252723 28.5000919061332 27.287216531888316 22.194630189451253 58-59 22.049693553735402 28.23468099752719 27.380557624959007 22.335067823778402 60-61 22.141396757506815 28.23417117734212 27.43084424368917 22.1935878214619 62-63 21.9609829066488 28.25874794188988 27.436594626319916 22.343674525141406 64-65 21.837324366493604 28.125980358548937 27.40656598708991 22.63012928786755 66-67 21.866219881059173 28.39908288094646 27.07441307445255 22.660284163541817 68-69 21.89590688934967 28.140054551258626 27.307915387106924 22.65612317228478 70-71 21.785430804842267 28.085299691228478 27.42692649741721 22.702343006512045 72-73 21.8848734394397 27.98416690932885 27.32945957031519 22.801500080916256 74-75 21.624069461828746 28.154985757353725 27.34767303445118 22.873271746366353 76-77 21.64944142639119 28.08438809359809 27.408338969456885 22.857831510553837 78-79 21.803696646885932 28.018952101524665 27.292766588608735 22.88458466298067 80-81 21.591037485542174 28.25557254549426 27.287622230805216 22.86576773815835 82-83 21.76280576055324 28.127191610812517 27.285768906685565 22.82423372194868 84-85 21.839112404488052 27.793852638490694 27.343987416241173 23.023047540780077 86-87 21.603135670958164 27.80160211759597 27.52479999501351 23.070462216432354 88-89 21.800023686982755 27.75005350349175 27.49063948620025 22.959283323325234 90-91 21.73535032417779 27.8454791045749 27.51001799876857 22.90915257247874 92-93 21.713327845310467 27.668983928056694 27.531272641340394 23.086415585292443 94-95 21.625414242408347 27.55622816479642 27.52441829485594 23.293939297939293 96-97 21.741486417786206 27.49425278999978 27.49492786323224 23.269332928981775 98-99 21.608790273288008 27.4186553228157 27.402355890830144 23.57019851306615 100-101 21.467437499594467 27.50679669605954 27.598154672687986 23.427611131658004 102-103 21.593864290984246 27.31780461657398 27.575604463305826 23.512726629135948 104-105 21.482925208820213 27.17116574686721 27.65411561055176 23.691793433760814 106-107 21.35623826625233 27.367005415236473 27.597281429834204 23.679474888677003 108-109 21.377196131273813 27.445447280260986 27.371915215686705 23.805441372778496 110-111 21.346338621588636 27.58772429695818 27.379202148359667 23.68673493309352 112-113 21.301893126037317 27.52038418612403 27.49491612284174 23.682806564996913 114-115 21.104275972423732 27.622189461322016 27.41879670926882 23.85473785698543 116-117 21.119178602420696 27.655897253658214 27.49695576297237 23.72796838094872 118-119 21.05535423709711 27.63697027521563 27.305967757768272 24.00170772991898 120-121 20.96134765042253 27.770335206541525 27.37056569398507 23.897751449050876 122-123 21.114481936549165 27.728648869504408 27.25491046084494 23.90195873310149 124-125 21.067056217065485 27.726208098626103 27.16467607935904 24.042059604949372 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 8514.0 1 4658.0 2 953.0 3 1071.0 4 949.0 5 790.5 6 664.5 7 610.0 8 651.5 9 651.0 10 564.5 11 514.0 12 490.5 13 494.0 14 554.0 15 612.5 16 651.5 17 750.0 18 899.0 19 1085.0 20 1381.0 21 1823.0 22 2573.0 23 3551.5 24 4663.0 25 6297.0 26 9215.0 27 12613.0 28 15701.5 29 19434.0 30 23536.5 31 27528.5 32 32842.0 33 39133.5 34 45849.5 35 51060.0 36 56112.5 37 61466.0 38 63653.0 39 64634.0 40 65358.0 41 66532.5 42 67779.5 43 69861.5 44 72318.5 45 73611.0 46 73212.5 47 72094.0 48 72022.5 49 70993.0 50 69144.5 51 66400.5 52 62791.5 53 60173.5 54 57509.5 55 52800.5 56 47026.0 57 42026.5 58 38576.0 59 34886.5 60 29669.5 61 24367.5 62 19628.5 63 14861.5 64 11044.0 65 8654.5 66 6525.5 67 4888.5 68 3613.0 69 2455.0 70 1839.5 71 1476.5 72 1255.0 73 1135.5 74 903.5 75 684.5 76 437.0 77 225.0 78 157.5 79 105.5 80 61.0 81 45.0 82 36.5 83 27.5 84 24.5 85 23.5 86 15.0 87 9.5 88 8.5 89 9.0 90 7.0 91 4.0 92 7.0 93 6.5 94 3.0 95 2.5 96 5.0 97 4.0 98 1.0 99 1.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4633231779978876 2 0.45437931392807784 3 0.44874416257773536 4 0.4461592307656516 5 0.45396572483814446 6 0.5117131015200951 7 0.5079907997106945 8 0.5175550474154043 9 0.5213290478610464 10-11 0.5405867898610702 12-13 0.5494531059765174 14-15 0.5277138294368933 16-17 0.5208637601348715 18-19 0.5327027478342149 20-21 0.5330387889697857 22-23 0.5426030366744956 24-25 0.5374331730503281 26-27 0.5437404066718123 28-29 0.5241724728543385 30-31 0.5297817748865603 32-33 0.5311517887469647 34-35 0.5297300762503186 36-37 0.4772818097831397 38-39 0.44471166895088476 40-41 0.44365184690793047 42-43 0.4561370675602948 44-45 0.4605056023227163 46-47 0.46539112344755457 48-49 0.4796857963683776 50-51 0.461513725729429 52-53 0.46055730095895797 54-55 0.4511739984810941 56-57 0.43473383215624156 58-59 0.45213042325156505 60-61 0.44822717621531866 62-63 0.4640986575415127 64-65 0.4637884657240627 66-67 0.47190515161400554 68-69 0.48123675545562783 70-71 0.47764370023683145 72-73 0.48893985225563735 74-75 0.4786518236435441 76-77 0.47588594660461453 78-79 0.48775078362207885 80-81 0.4803578786395194 82-83 0.4825033720435489 84-85 0.4790395634153567 86-87 0.46968211025561357 88-89 0.474541782062331 90-91 0.4589546432354661 92-93 0.45918728709855366 94-95 0.45412082074686955 96-97 0.44305731259115116 98-99 0.4052914588166079 100-101 0.4032235133669409 102-103 0.3928320874823643 104-105 0.3975366633803567 106-107 0.3973557181535108 108-109 0.4092722538072168 110-111 0.4318128592085869 112-113 0.4314509687548952 114-115 0.4244716528622692 116-117 0.41909499469313494 118-119 0.4613586298207039 120-121 0.45507724551734047 122-123 0.41971537832803507 124-125 0.42940887262334904 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1934287.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.39895083467773 #Duplication Level Percentage of deduplicated Percentage of total 1 83.79909523766744 55.64172004676357 2 9.92334219227113 13.177990206805879 3 2.8631519545702746 5.703308575911693 4 1.1638860414310779 3.0912324816859926 5 0.6186210166137974 2.0537893233718947 6 0.3515320295033352 1.4004814766283857 7 0.2337939319886037 1.08665702568901 8 0.16035191833119464 0.8517759313215402 9 0.12057412519499049 0.7205395869680805 >10 0.668257843717377 8.564597474741829 >50 0.0628095565738634 2.9263559087600974 >100 0.03424003123086798 3.831707927012785 >500 1.7206045303909778E-4 0.05803713849463197 >1k 0.0 0.0 >5k 1.7206045303909778E-4 0.8918068958446795 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 7806 0.40355955450251174 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 5.169863624167458E-5 0.0 0.0 0.0 16-17 0.0 1.0339727248334916E-4 0.0 0.0 0.0 18-19 0.0 1.0339727248334916E-4 0.0 7.754795436251186E-5 0.0 20-21 0.0 1.0339727248334916E-4 0.0 1.0339727248334916E-4 0.0 22-23 0.0 1.0339727248334916E-4 0.0 2.3264386308753562E-4 0.0 24-25 0.0 1.0339727248334916E-4 0.0 4.6528772617507124E-4 0.0 26-27 0.0 1.0339727248334916E-4 0.0 0.0012924659060418644 0.0 28-29 0.0 1.0339727248334916E-4 0.0 0.0035413565825547087 0.0 30-31 0.0 1.0339727248334916E-4 0.0 0.010365576566455753 0.0 32-33 0.0 1.0339727248334916E-4 0.0 0.025901016757078964 0.0 34-35 0.0 1.0339727248334916E-4 0.0 0.05092315669804946 0.0 36-37 0.0 1.0339727248334916E-4 0.0 0.08967128456118456 0.0 38-39 0.0 1.5509590872502376E-4 0.0 0.14783225033306846 0.0 40-41 0.0 1.5509590872502376E-4 0.0 0.20935362746066122 0.0 42-43 0.0 1.5509590872502376E-4 0.0 0.2727103061748334 0.0 44-45 0.0 1.5509590872502376E-4 0.0 0.3531792334849999 0.0 46-47 0.0 1.5509590872502376E-4 0.0 0.44946794348511876 0.0 48-49 0.0 1.5509590872502376E-4 0.0 0.5545971202825641 0.0 50-51 0.0 1.5509590872502376E-4 0.0 0.6660593800196144 0.0 52-53 0.0 1.5509590872502376E-4 0.0 0.7736442420385392 0.0 54-55 0.0 1.5509590872502376E-4 0.0 0.8985222978803042 0.0 56-57 0.0 1.5509590872502376E-4 0.0 1.049947603432169 0.0 58-59 0.0 1.5509590872502376E-4 0.0 1.2233965280229873 0.0 60-61 0.0 1.5509590872502376E-4 0.0 1.412975427121208 0.0 62-63 0.0 1.5509590872502376E-4 0.0 1.5994007094086866 0.0 64-65 0.0 1.5509590872502376E-4 0.0 1.796139869626379 0.0 66-67 0.0 1.5509590872502376E-4 0.0 2.0260178556749855 0.0 68-69 0.0 1.5509590872502376E-4 0.0 2.2945405723142427 0.0 70-71 0.0 1.5509590872502376E-4 0.0 2.5981925122797187 0.0 72-73 0.0 1.5509590872502376E-4 0.0 2.900138397249219 0.0 74-75 0.0 1.5509590872502376E-4 0.0 3.2066854608442283 0.0 76-77 0.0 1.5509590872502376E-4 0.0 3.549499117762773 0.0 78-79 0.0 1.5509590872502376E-4 0.0 3.940702698203524 0.0 80-81 0.0 1.5509590872502376E-4 0.0 4.374480105589294 0.0 82-83 0.0 1.5509590872502376E-4 0.0 4.82198350089723 0.0 84-85 0.0 1.5509590872502376E-4 0.0 5.263412306446769 0.0 86-87 0.0 1.5509590872502376E-4 0.0 5.730819676707748 0.0 88-89 0.0 1.5509590872502376E-4 0.0 6.246823765035902 0.0 90-91 0.0 2.067945449666983E-4 0.0 6.815689708921168 0.0 92-93 0.0 2.584931812083729E-4 0.0 7.41898694454339 0.0 94-95 0.0 2.584931812083729E-4 0.0 8.026859509472999 0.0 96-97 0.0 2.584931812083729E-4 0.0 8.631630156228109 0.0 98-99 0.0 2.584931812083729E-4 0.0 9.288151137861135 0.0 100-101 0.0 2.843424993292102E-4 0.0 10.016481525233846 0.0 102-103 0.0 3.101918174500475E-4 0.0 10.77544335457975 0.0 104-105 0.0 3.101918174500475E-4 0.0 11.526081703490743 0.0 106-107 0.0 3.101918174500475E-4 0.0 12.279149888305096 0.0 108-109 0.0 3.101918174500475E-4 0.0 13.049382020351686 0.0 110-111 0.0 3.101918174500475E-4 0.0 13.886925776784935 0.0 112-113 0.0 3.101918174500475E-4 0.0 14.763165962445077 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2580 0.0 57.432667 1 GTATCAA 4535 0.0 48.026745 1 TCAACGC 5155 0.0 40.880108 4 ATCAACG 5195 0.0 40.78498 3 CAACGCA 5375 0.0 39.20891 5 AACGCAG 5585 0.0 37.84319 6 TATCAAC 6000 0.0 35.707916 2 GTACATG 11290 0.0 35.63134 1 TACATGG 11345 0.0 34.83187 2 ACATGGG 11445 0.0 32.86526 3 ACGCAGA 6665 0.0 31.534046 7 CGCAGAG 6725 0.0 31.075632 8 CATGGGA 7600 0.0 29.530117 4 GAGTACA 8305 0.0 25.795399 1 CATGGGG 3925 0.0 25.783785 4 GCAGAGT 7750 0.0 25.429127 9 AGTACAT 8145 0.0 25.135098 2 ATGGGAG 3140 0.0 24.457947 5 GTATAGG 785 0.0 21.984049 1 ATGGGAT 3410 0.0 21.82306 5 >>END_MODULE