FastQCFastQC Report
Thu 26 May 2016
SRR1364104_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364104_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1934287
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84210.4353542157911417No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83070.42946057125959075No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42400.21920221766470022No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27560.14248144148205516No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49100.067.604731
GTATCAA89100.050.430931
ATCAACG111450.039.9829443
TCAACGC112050.039.7188264
CAACGCA115050.038.631425
TGGTATC22150.038.140612
TATCAAC116900.038.1188962
AACGCAG119900.037.168016
GTGGTAT25350.034.087361
ACGCAGA140300.031.6829597
GTACATG104900.031.188911
CGCAGAG142500.031.0267838
TACATGG105950.030.771782
ACATGGG108450.028.9652543
GCAGAGT156300.028.1350889
GAGTACT99500.026.79139312-13
CATGGGA75850.026.1213344
CAGAGTA153300.024.9007110-11
CTAGACG1701.4005032E-524.497523
GTACTTT108850.024.4595714-15
Produced by FastQC (version 0.11.5)