##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364104_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1934287 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.12096808798281 33.0 31.0 34.0 30.0 34.0 2 32.13520589240377 33.0 31.0 34.0 30.0 34.0 3 32.27504036371025 34.0 31.0 34.0 30.0 34.0 4 35.90573684256783 37.0 35.0 37.0 35.0 37.0 5 35.846125730049366 37.0 35.0 37.0 35.0 37.0 6 35.884403917309065 37.0 35.0 37.0 35.0 37.0 7 35.80959392272191 37.0 35.0 37.0 33.0 37.0 8 35.79267554401182 37.0 35.0 37.0 33.0 37.0 9 37.4509661699634 39.0 37.0 39.0 34.0 39.0 10-11 37.38073357262908 39.0 37.0 39.0 34.0 39.0 12-13 37.3277820199381 39.0 37.0 39.0 34.0 39.0 14-15 38.61910719557129 40.0 38.0 41.0 34.0 41.0 16-17 38.60108479248426 40.0 38.0 41.0 34.0 41.0 18-19 38.75731445230206 40.0 38.0 41.0 34.0 41.0 20-21 38.82528058142354 40.0 38.0 41.0 34.0 41.0 22-23 38.79478303891822 40.0 38.0 41.0 34.0 41.0 24-25 38.76477534099128 40.0 38.0 41.0 34.0 41.0 26-27 38.646329370977526 40.0 38.0 41.0 34.0 41.0 28-29 38.56776605539922 40.0 38.0 41.0 34.0 41.0 30-31 38.422350457817274 40.0 38.0 41.0 34.0 41.0 32-33 38.261417773060565 40.0 37.5 41.0 34.0 41.0 34-35 38.13690522657703 40.0 37.0 41.0 33.0 41.0 36-37 38.01534157030471 40.0 37.0 41.0 33.0 41.0 38-39 37.910060141023536 40.0 37.0 41.0 33.0 41.0 40-41 37.77540535608212 40.0 37.0 41.0 33.0 41.0 42-43 37.620137549391586 40.0 37.0 41.0 33.0 41.0 44-45 37.41351386841767 40.0 37.0 41.0 32.5 41.0 46-47 37.093792958335555 40.0 37.0 41.0 31.0 41.0 48-49 36.78039944434306 40.0 36.0 41.0 30.5 41.0 50-51 36.58886168391764 40.0 36.0 41.0 30.0 41.0 52-53 36.447039658540845 40.0 36.0 41.0 30.0 41.0 54-55 36.28715412966122 39.5 35.5 41.0 30.0 41.0 56-57 36.11320967364202 39.0 35.0 41.0 29.0 41.0 58-59 35.924484836014514 39.0 35.0 41.0 29.0 41.0 60-61 35.71328479176048 39.0 35.0 41.0 28.5 41.0 62-63 35.47698092372022 38.5 35.0 40.5 28.0 41.0 64-65 35.28185243451463 38.0 35.0 40.0 28.0 41.0 66-67 35.086481220211894 37.5 35.0 40.0 28.0 41.0 68-69 34.79035065634004 37.0 34.0 40.0 28.0 41.0 70-71 34.48272490069984 36.5 34.0 39.0 28.0 41.0 72-73 34.15344491277665 36.0 34.0 39.0 28.0 41.0 74-75 33.86940562594899 36.0 34.0 39.0 28.0 40.0 76-77 33.65883320313893 35.0 34.0 37.5 28.0 39.5 78-79 33.35843776027032 35.0 34.0 37.0 28.0 39.0 80-81 33.06554430650674 35.0 34.0 36.5 27.5 39.0 82-83 32.78451258784244 35.0 34.0 36.0 27.0 38.0 84-85 32.55556026587575 35.0 34.0 36.0 27.0 37.0 86-87 32.35456062104538 35.0 34.0 35.5 26.5 37.0 88-89 32.193928563858414 35.0 33.0 35.0 26.0 36.0 90-91 32.05534339009671 35.0 33.0 35.0 26.0 36.0 92-93 31.94457130715349 35.0 33.0 35.0 26.0 36.0 94-95 31.853015607301295 35.0 33.0 35.0 26.0 36.0 96-97 31.785433082060727 35.0 33.0 35.0 26.0 35.5 98-99 31.719205578076057 35.0 33.0 35.0 25.0 35.0 100-101 31.657319725562957 35.0 33.0 35.0 25.0 35.0 102-103 30.48428981841888 34.0 31.0 35.0 23.5 35.0 104-105 31.401925877597275 35.0 33.0 35.0 25.0 35.0 106-107 31.54448228210188 35.0 33.0 35.0 25.0 35.0 108-109 31.540063082675942 35.0 33.0 35.0 25.0 35.0 110-111 31.497434196683326 35.0 33.0 35.0 25.0 35.0 112-113 31.438170240507226 35.0 33.0 35.0 25.0 35.0 114-115 31.368766113818683 35.0 33.0 35.0 24.5 35.0 116-117 31.301855153862896 35.0 33.0 35.0 24.0 35.0 118-119 31.214749414125205 35.0 33.0 35.0 24.0 35.0 120-121 31.098270318727263 35.0 33.0 35.0 23.0 35.0 122-123 30.960426244916086 35.0 33.0 35.0 19.5 35.0 124-125 30.12518307779559 34.5 31.5 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.19032968680753726 1101 2 0.20795779202160247 1101 3 0.15550470911752257 1101 4 0.16302079432880134 1101 5 0.19713291883756767 1101 6 0.0989439818175768 1101 7 0.18193202783481865 1101 8 0.1327107333492492 1101 9 0.1814853650030912 1101 10-11 0.2886843907872034 1101 12-13 0.18593122762192138 1101 14-15 0.3288236925222563 1101 16-17 0.3384950932632904 1101 18-19 0.38803978045642396 1101 20-21 0.30440677069900346 1101 22-23 0.37190723611630716 1101 24-25 0.3969510608672451 1101 26-27 0.4272825163027676 1101 28-29 0.47391038124469986 1101 30-31 0.45000947523331547 1101 32-33 0.49436183344093365 1101 34-35 0.48051836994386576 1101 36-37 0.5524151731927631 1101 38-39 0.4661958163291402 1101 40-41 0.43928727405926793 1101 42-43 0.4654049511128733 1101 44-45 0.46792553199322384 1101 46-47 0.5528065298258298 1101 48-49 0.5930365894577605 1101 50-51 0.589681301056558 1101 52-53 0.6035785510733334 1101 54-55 0.5984927723743425 1101 56-57 0.6502031429844521 1101 58-59 0.6472076420478885 1101 60-61 0.6738268073550273 1101 62-63 0.5688117704381384 1101 64-65 0.5639324234902148 1101 66-67 0.5254202704813977 1101 68-69 0.511873298856095 1101 70-71 0.44253656598026225 1101 72-73 0.47696402326381815 1101 74-75 0.43656375026608174 1101 76-77 0.5036555199641555 1101 78-79 0.45490322390600824 1101 80-81 0.4200881237637404 1101 82-83 0.47050658767965814 1101 84-85 0.426500998996282 1101 86-87 0.4185940748598327 1101 88-89 0.3707196088921165 1101 90-91 0.4335114851951758 1101 92-93 0.4332870597839573 1101 94-95 0.441290554131907 1101 96-97 0.4268343475528873 1101 98-99 0.49793561626131577 1101 100-101 0.48978626340207754 1101 102-103 0.5026053308353475 1101 104-105 0.43162752357129364 1101 106-107 0.5207865224700363 1101 108-109 0.5325741046750885 1101 110-111 0.46538677294012487 1101 112-113 0.5255956558263719 1101 114-115 0.5391126026401238 1101 116-117 0.508737274045437 1101 118-119 0.6237341166639538 1101 120-121 0.6167537277397095 1101 122-123 0.6201357707167396 1101 124-125 0.7098270916907019 1102 1 0.2454796603804752 1102 2 0.2769737143260471 1102 3 0.21655122074542987 1102 4 0.10161091060787442 1102 5 0.1588219383935936 1102 6 0.21491748868861293 1102 7 0.20015018322594358 1102 8 0.18683665829639295 1102 9 0.38069585654643134 1102 10-11 0.29254109004724427 1102 12-13 0.31059263089888134 1102 14-15 0.366061073600477 1102 16-17 0.28954722064172955 1102 18-19 0.33067654049870043 1102 20-21 0.315122118214866 1102 22-23 0.3889237860634651 1102 24-25 0.3498324363957863 1102 26-27 0.259635846112495 1102 28-29 0.315240653443432 1102 30-31 0.3635929847470578 1102 32-33 0.27651200521682995 1102 34-35 0.3294835853244109 1102 36-37 0.3217358325479438 1102 38-39 0.4133499521642321 1102 40-41 0.3428037249049254 1102 42-43 0.21464104387185756 1102 44-45 0.31096943394882715 1102 46-47 0.3977622644981267 1102 48-49 0.392752498548667 1102 50-51 0.37404060998891 1102 52-53 0.44220004948770253 1102 54-55 0.4672452499113433 1102 56-57 0.5189349743798033 1102 58-59 0.5271090360753732 1102 60-61 0.508979753972369 1102 62-63 0.4750163489265091 1102 64-65 0.37404572951558634 1102 66-67 0.379955814878862 1102 68-69 0.4530276659279835 1102 70-71 0.46170857311556546 1102 72-73 0.4001645055576901 1102 74-75 0.37415046406101027 1102 76-77 0.3281674451755734 1102 78-79 0.3346477072569627 1102 80-81 0.34043530928171606 1102 82-83 0.31184511833505013 1102 84-85 0.286619260095641 1102 86-87 0.2457652231156473 1102 88-89 0.2777128699914755 1102 90-91 0.2526471551211742 1102 92-93 0.2347867955133367 1102 94-95 0.30714431338137516 1102 96-97 0.285767520121599 1102 98-99 0.3429904524135381 1102 100-101 0.32877113390947343 1102 102-103 0.36745155837234833 1102 104-105 0.37088657483979404 1102 106-107 0.32293041781887055 1102 108-109 0.2724203322120893 1102 110-111 0.32168962706274584 1102 112-113 0.3339003598432839 1102 114-115 0.40758842187902644 1102 116-117 0.40242203223782624 1102 118-119 0.3545455926153238 1102 120-121 0.38713559877565373 1102 122-123 0.49694437272519565 1102 124-125 0.5058390829697714 1103 1 0.18927661876452362 1103 2 0.17329186981242373 1103 3 0.15424432064244087 1103 4 0.07603053813962646 1103 5 0.09989489602084944 1103 6 0.13823769025369614 1103 7 0.1488941074059511 1103 8 0.24397594183726312 1103 9 0.25041803650773176 1103 10-11 0.18706307515319764 1103 12-13 0.28131178660372314 1103 14-15 0.2729348691642528 1103 16-17 0.2538594582989404 1103 18-19 0.3434169888805698 1103 20-21 0.4051812160482413 1103 22-23 0.4194950716211494 1103 24-25 0.3202665137488765 1103 26-27 0.3587724067497646 1103 28-29 0.35407555400673374 1103 30-31 0.41671227952282663 1103 32-33 0.4177527549515645 1103 34-35 0.3847754368013341 1103 36-37 0.38135024472455115 1103 38-39 0.397138216176657 1103 40-41 0.3238681100670533 1103 42-43 0.3012387285260729 1103 44-45 0.34945537715404384 1103 46-47 0.4657326968078408 1103 48-49 0.3786125635394626 1103 50-51 0.34696645621507116 1103 52-53 0.4608930703893108 1103 54-55 0.3775490677303637 1103 56-57 0.4155152288425512 1103 58-59 0.46584218128258925 1103 60-61 0.43655961248281017 1103 62-63 0.3258084607957201 1103 64-65 0.33318840010666406 1103 66-67 0.2803357730600524 1103 68-69 0.36208840315998003 1103 70-71 0.3080673112760053 1103 72-73 0.38271218806634266 1103 74-75 0.34415104189781687 1103 76-77 0.3115528691566283 1103 78-79 0.3012253943688137 1103 80-81 0.2646659085935781 1103 82-83 0.2651378616799249 1103 84-85 0.1737545646083376 1103 86-87 0.0805461351908221 1103 88-89 0.008354117689890472 1103 90-91 0.07150793155830826 1103 92-93 0.13443409562568576 1103 94-95 0.11081249217627587 1103 96-97 0.12737763436262028 1103 98-99 0.14159433040560998 1103 100-101 0.2199833575230521 1103 102-103 0.3097737528649027 1103 104-105 0.219665583710686 1103 106-107 0.189522526469748 1103 108-109 0.1793113775457016 1103 110-111 0.16815058382397297 1103 112-113 0.2339716507648575 1103 114-115 0.2384458341843647 1103 116-117 0.278040402962322 1103 118-119 0.30982010898785717 1103 120-121 0.32033010498605563 1103 122-123 0.3184385124358968 1103 124-125 0.4928108742931485 1104 1 0.2231741625374255 1104 2 0.25040462254465723 1104 3 0.14432512165262068 1104 4 0.09645346120103682 1104 5 0.11997429081699096 1104 6 0.14466471121922098 1104 7 0.1894472118689663 1104 8 0.1843055133684004 1104 9 0.19890592521990413 1104 10-11 0.2231427660448304 1104 12-13 0.264930171738186 1104 14-15 0.2515714955537902 1104 16-17 0.2769576616269518 1104 18-19 0.2945352404606467 1104 20-21 0.22613393683283078 1104 22-23 0.27970949171118065 1104 24-25 0.21375688070366294 1104 26-27 0.30918671285739663 1104 28-29 0.2617637117920921 1104 30-31 0.39209574833900973 1104 32-33 0.38656774294125285 1104 34-35 0.34757071479691604 1104 36-37 0.37234819912632133 1104 38-39 0.43625540123801443 1104 40-41 0.4240082763069495 1104 42-43 0.3765821549785642 1104 44-45 0.3579744109915879 1104 46-47 0.3019945846465788 1104 48-49 0.4704408549846235 1104 50-51 0.47012858598490936 1104 52-53 0.5268690032877501 1104 54-55 0.5440909239185103 1104 56-57 0.46525879241887935 1104 58-59 0.5251323689989889 1104 60-61 0.5105283269756029 1104 62-63 0.49376934744361023 1104 64-65 0.5236422431075383 1104 66-67 0.544367728854624 1104 68-69 0.48737344897213575 1104 70-71 0.3518160191521673 1104 72-73 0.4852972382624401 1104 74-75 0.4499307971590838 1104 76-77 0.4610445000271213 1104 78-79 0.48390756915666344 1104 80-81 0.48873527399338457 1104 82-83 0.4325064375636245 1104 84-85 0.4135725090013125 1104 86-87 0.4201249034192003 1104 88-89 0.5397465881923296 1104 90-91 0.539273050215705 1104 92-93 0.49510912866693246 1104 94-95 0.4530111425461918 1104 96-97 0.5379330194316942 1104 98-99 0.5149502038885103 1104 100-101 0.42963753188257314 1104 102-103 0.4752555584437168 1104 104-105 0.5718965051354203 1104 106-107 0.5161057929060995 1104 108-109 0.5825806215367741 1104 110-111 0.5630117698972299 1104 112-113 0.5637651936312693 1104 114-115 0.48503887943786594 1104 116-117 0.48486502076316995 1104 118-119 0.46192335719852906 1104 120-121 0.5026141918416904 1104 122-123 0.5856833798145828 1104 124-125 0.6517101249272059 1105 1 0.09713174756274157 1105 2 0.1784902760098035 1105 3 0.12404946881831336 1105 4 0.05094736821124002 1105 5 0.06517919415902895 1105 6 0.08910747124897256 1105 7 0.11692916567481859 1105 8 0.028661031584285013 1105 9 0.08219285836206325 1105 10-11 0.15141962774553264 1105 12-13 0.0950705068465183 1105 14-15 0.19079246136566042 1105 16-17 0.20991790253954434 1105 18-19 0.17195841362732978 1105 20-21 0.15785144647026073 1105 22-23 0.19525657955106368 1105 24-25 0.14518831152319933 1105 26-27 0.15576398763634813 1105 28-29 0.12650858083401317 1105 30-31 0.1145122487783965 1105 32-33 0.13733856642279818 1105 34-35 0.2875763190075986 1105 36-37 0.26050311789705205 1105 38-39 0.27725034913499513 1105 40-41 0.27946683131061434 1105 42-43 0.31754001043394453 1105 44-45 0.3617488175491914 1105 46-47 0.36422430740441314 1105 48-49 0.3158981434171153 1105 50-51 0.32790010045683005 1105 52-53 0.4811567868419786 1105 54-55 0.4242116736308361 1105 56-57 0.3541853904624901 1105 58-59 0.4022928142256532 1105 60-61 0.4186129143692625 1105 62-63 0.42095434165600665 1105 64-65 0.40162987178798915 1105 66-67 0.4515379643341717 1105 68-69 0.42511267321440727 1105 70-71 0.3930107576492361 1105 72-73 0.4293210273054697 1105 74-75 0.3594839224845927 1105 76-77 0.2477986595397752 1105 78-79 0.26443462414394503 1105 80-81 0.29171108052781136 1105 82-83 0.2906677038609402 1105 84-85 0.3361863549226811 1105 86-87 0.314568054667852 1105 88-89 0.2929586175865637 1105 90-91 0.28473442199542376 1105 92-93 0.32101794238854353 1105 94-95 0.3302561888591775 1105 96-97 0.3243087969181211 1105 98-99 0.3338719703787518 1105 100-101 0.30891239298764006 1105 102-103 0.26572568619135595 1105 104-105 0.21647584946233067 1105 106-107 0.23832876224774324 1105 108-109 0.2645922788818318 1105 110-111 0.28859861046721846 1105 112-113 0.2654288764388113 1105 114-115 0.30679637164649876 1105 116-117 0.2868768394860268 1105 118-119 0.28101579293226564 1105 120-121 0.327868202935683 1105 122-123 0.3081804468954168 1105 124-125 0.3531517910111148 1106 1 0.06553963512795491 1106 2 0.13111670675028364 1106 3 0.0339123318565413 1106 4 0.05438868838565014 1106 5 0.08575533485824138 1106 6 0.05038939525427111 1106 7 0.10748919837745774 1106 8 0.11112643102366349 1106 9 0.12139977068784447 1106 10-11 0.1496986026735101 1106 12-13 0.1519357495857534 1106 14-15 0.13011568218634295 1106 16-17 0.1596150868033419 1106 18-19 0.16440444453910885 1106 20-21 0.1446320613966776 1106 22-23 0.1647326560129514 1106 24-25 0.18866213967861967 1106 26-27 0.18034291681956915 1106 28-29 0.19261596909999668 1106 30-31 0.20341306050463004 1106 32-33 0.1589693915804702 1106 34-35 0.15547395779915973 1106 36-37 0.09089224037622756 1106 38-39 0.151394459739997 1106 40-41 0.0888027779352285 1106 42-43 0.0649527547304487 1106 44-45 0.1523086069286208 1106 46-47 0.15863663318499732 1106 48-49 0.052064367235537645 1106 50-51 -0.003196605920180673 1106 52-53 0.10361892575033238 1106 54-55 0.06804622844668984 1106 56-57 0.12457733549091188 1106 58-59 0.10957036814607335 1106 60-61 0.1158689585178223 1106 62-63 0.01698683503762055 1106 64-65 0.04242483305093714 1106 66-67 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-0.9072307236609305 2216 72-73 -0.95779119580029 2216 74-75 -0.7460240411721202 2216 76-77 -0.5820109680162844 2216 78-79 -0.5117724799230103 2216 80-81 -0.4364038637427612 2216 82-83 -0.5061135602281084 2216 84-85 -0.4964148517202176 2216 86-87 -0.5351912276698059 2216 88-89 -0.5724096758747663 2216 90-91 -0.5782424140866809 2216 92-93 -0.5671345773220011 2216 94-95 -0.6658080991800581 2216 96-97 -0.6683030591408077 2216 98-99 -0.6148902358374997 2216 100-101 -0.7395754256394547 2216 102-103 -0.8060366305058935 2216 104-105 -0.7780620355029484 2216 106-107 -0.8718823298460059 2216 108-109 -0.7844508703938615 2216 110-111 -0.7958468552597715 2216 112-113 -0.8162734076219138 2216 114-115 -0.9430051295066093 2216 116-117 -1.0153622255234147 2216 118-119 -1.0406854815265305 2216 120-121 -1.2443050951362942 2216 122-123 -1.2943506291081697 2216 124-125 -1.661844962989754 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10.0 3 17.0 4 47.0 5 103.0 6 323.0 7 798.0 8 1626.0 9 2795.0 10 4566.0 11 6613.0 12 8671.0 13 12778.0 14 19240.0 15 17900.0 16 7617.0 17 5429.0 18 5219.0 19 4783.0 20 4748.0 21 4836.0 22 5097.0 23 5933.0 24 7289.0 25 9858.0 26 13226.0 27 16932.0 28 22204.0 29 28858.0 30 37095.0 31 46359.0 32 59936.0 33 77051.0 34 104506.0 35 153534.0 36 284769.0 37 611862.0 38 339844.0 39 1815.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.659281914356484 19.374682491555117 13.71170414453062 23.25433144955778 2 15.939015437428267 20.750157683041575 39.81290002378171 23.497926855748453 3 19.321796610327215 25.524288794785882 30.254610613626625 24.899303981260278 4 12.653965001057237 17.156606025889644 37.51134138832552 32.6780875847276 5 12.651224973336427 38.5388517836288 34.730626840794564 14.079296402240205 6 31.04301481631216 37.33877134055081 17.392145012606715 14.22606883053032 7 27.100270021977092 33.695051458237586 21.241780563070527 17.962897956714798 8 25.825381652257395 35.644710428183615 19.74179633115458 18.788111588404412 9 26.469146754926626 15.671053815206232 20.331636583214685 37.52816284665246 10-11 24.518527862621468 26.96600691938482 27.797816857573583 20.717648360420128 12-13 25.508773834636223 24.703514704057575 28.332247590685363 21.45546387062084 14-15 22.573744227201033 25.842700695398356 26.936437043727224 24.64711803367339 16-17 21.31654189583253 29.248306669227482 27.48961564241362 21.945535792526364 18-19 21.01557318019508 27.136536615300628 30.84014936769983 21.007740836804466 20-21 22.083046621313176 26.155684239205456 30.990127111436927 20.771142028044444 22-23 21.601628920630702 26.40934359792523 30.73535623203795 21.25367124940611 24-25 21.334993204214268 26.42361242152793 31.04903770743432 21.192356666823486 26-27 21.23997111080207 26.687482260905437 30.9103302663979 21.16221636189459 28-29 21.05116769124747 26.63505984375638 31.08256427303704 21.231208191959105 30-31 21.32524801128271 26.384011266166812 31.10637149502633 21.184369227524147 32-33 21.185790940020794 26.49495653954144 30.95685903901541 21.362393481422355 34-35 21.57407354751389 26.5826115772892 30.737941163850042 21.105373711346868 36-37 21.667337887293872 26.277667171417683 30.616940505726397 21.438054435562044 38-39 21.379169688882776 26.53753036648646 30.711316366185574 21.37198357844518 40-41 21.6052219758495 26.405181857707777 30.67502392354392 21.314572242898805 42-43 21.541710201226604 26.35278528987684 30.45365036315707 21.65185414573949 44-45 21.597518879049492 26.604867840191243 30.12451099552445 21.67310228523482 46-47 21.735424991224157 26.790414245662614 29.532251418739826 21.941909344373407 48-49 21.847817826413557 26.98513715906688 29.188119446597117 21.978925567922445 50-51 21.94914715344724 27.108360858548913 28.829615253579227 22.11287673442462 52-53 22.005136776496972 27.28542868767665 28.667178138507882 22.042256397318496 54-55 21.98006293791976 27.171717537263085 28.644068848107857 22.2041506767093 56-57 21.87630377498272 27.428789005974814 28.530150903149327 22.164756315893143 58-59 21.909804491267327 27.10771462559589 28.732680310626087 22.249800572510697 60-61 22.01009984557617 27.05937640070993 28.942654321721644 21.987869431992255 62-63 21.871133911358555 27.05803223616764 28.96746966711765 22.103364185356156 64-65 21.785882860196033 26.954764210274895 28.914504414288057 22.34484851524102 66-67 21.8071826983276 27.215015145115483 28.576860620993678 22.400941535563234 68-69 21.826698933508833 27.001396380164888 28.686771921643476 22.4851327646828 70-71 21.72632603124562 27.0041364078857 28.790324290035553 22.479213270833128 72-73 21.826181947146416 26.918781959450694 28.62282070861253 22.632215384790364 74-75 21.665218243207963 27.08605289701063 28.53025430042181 22.7184745593596 76-77 21.548896311664194 27.09890000811669 28.58707110165141 22.76513257856771 78-79 21.6373526782737 27.049217618688438 28.396690873691444 22.91673882934642 80-81 21.373198496396864 27.25006682048734 28.419309027047174 22.95742565606862 82-83 21.597622276321975 27.108645201048244 28.357270663557166 22.93646185907262 84-85 21.628098622386442 26.890140914972804 28.239371923608026 23.242388539032728 86-87 21.33008183377131 26.846688211211678 28.417060136370665 23.406169818646354 88-89 21.483833577954048 26.789225177029056 28.40367018958407 23.32327105543283 90-91 21.405406746775427 26.928708097609093 28.400671668682055 23.26521348693343 92-93 21.339801177384743 26.74057676032564 28.424272096126373 23.495349966163243 94-95 21.228823121445856 26.63741709742702 28.385075843721168 23.748683937405957 96-97 21.287415118980775 26.625289916439954 28.35157881022326 23.73571615435601 98-99 21.113197768124298 26.51806451863063 28.267685495263663 24.10105221798141 100-101 20.952577905879615 26.567307319469037 28.431232147242437 24.04888262740891 102-103 21.074457610131763 26.36190414149146 28.413042225070956 24.150596023305827 104-105 20.97826131917041 26.232146402600947 28.453867090230727 24.335725187997916 106-107 20.773940547491737 26.404524012291958 28.39069529826826 24.430840141948046 108-109 20.836241077996505 26.378128761268453 28.13244577632581 24.653184384409233 110-111 20.7028325301454 26.454269995242925 28.166585658441747 24.67631181616993 112-113 20.607432535506607 26.360575965911586 28.222433091408345 24.80955840717346 114-115 20.356742081447965 26.371816418875245 28.174996767937944 25.09644473173885 116-117 20.334947281652855 26.31521791405066 28.31457978132836 25.035255022968123 118-119 20.212278041110192 26.317999268862597 28.18196014111883 25.28776254890839 120-121 19.994430751147917 26.420371764166873 28.22665446637469 25.35854301831052 122-123 20.124105063762713 26.428608176859797 28.023742422789166 25.42354433658832 124-125 19.908402156672903 26.360452896138696 28.0288883517171 25.702256595471297 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 808.0 1 538.0 2 368.0 3 467.5 4 316.5 5 235.0 6 586.5 7 1367.5 8 2199.5 9 2593.0 10 2143.5 11 1486.5 12 1400.5 13 1502.5 14 1660.0 15 1951.5 16 2210.0 17 2470.5 18 2765.0 19 3030.0 20 3403.5 21 3876.0 22 4526.0 23 5444.5 24 6628.0 25 8226.5 26 10879.5 27 14138.0 28 17134.5 29 20285.5 30 23997.0 31 28127.0 32 33142.0 33 39092.0 34 45464.0 35 50265.0 36 55343.0 37 60198.0 38 61894.5 39 63130.0 40 64336.5 41 65726.0 42 66918.0 43 68707.0 44 70966.5 45 72283.5 46 72067.0 47 71553.0 48 72254.5 49 71402.0 50 68666.0 51 65502.0 52 62301.0 53 59821.5 54 56828.0 55 51905.0 56 46138.5 57 41124.0 58 37876.5 59 34139.5 60 28930.0 61 23594.5 62 18934.0 63 14430.5 64 10750.5 65 8395.0 66 6169.5 67 4673.0 68 3356.0 69 2198.5 70 1698.5 71 1339.5 72 1156.5 73 1126.5 74 841.0 75 558.5 76 360.5 77 173.5 78 98.0 79 45.0 80 19.0 81 13.5 82 7.0 83 4.0 84 3.0 85 1.5 86 0.5 87 1.0 88 1.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16729678687805893 2 0.0013958631785252137 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 5.169863624167458E-5 10-11 5.686849986584204E-4 12-13 0.006565726802692672 14-15 0.0 16-17 3.8773977181255935E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 6.979315892626069E-4 96-97 0.005635151350342529 98-99 0.002042096131546146 100-101 0.003205315446983824 102-103 0.0047821238523548994 104-105 0.011528795881893431 106-107 0.01757753632216936 108-109 0.026598948346341574 110-111 0.01690545405102759 112-113 0.025435729030903895 114-115 0.027762167661779247 116-117 0.02688329084567078 118-119 0.01623337177988582 120-121 0.023445331535599422 122-123 0.01450146746578972 124-125 0.01496675519196479 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1934287.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.43838965243839 #Duplication Level Percentage of deduplicated Percentage of total 1 82.93015724584468 51.78025472054036 2 10.3578583017651 12.934559852207062 3 3.045352286340479 5.704406780504128 4 1.244009706932454 3.10695851251457 5 0.63376038301118 1.9785488870365326 6 0.40497878198071213 1.5171733794168958 7 0.24995223459963314 1.092463051290071 8 0.16599077909909835 0.8291357555281061 9 0.11523062689034248 0.6475333303506553 >10 0.7310638931480814 8.855861414951518 >50 0.07471352986034617 3.2527958058090465 >100 0.043944036166064654 4.66873151265495 >500 0.0021093136671488477 0.8717584722635455 >1k 5.273284167872119E-4 0.9238905727703042 >5k 3.515522778581413E-4 1.8359279521622296 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8421 0.4353542157911417 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8307 0.42946057125959075 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4240 0.21920221766470022 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2756 0.14248144148205516 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 5.169863624167458E-5 0.0 16-17 0.0 0.0 0.0 5.169863624167458E-5 0.0 18-19 0.0 0.0 0.0 1.2924659060418645E-4 0.0 20-21 0.0 0.0 0.0 1.5509590872502376E-4 0.0 22-23 0.0 0.0 0.0 3.1019181745004746E-4 0.0 24-25 0.0 0.0 0.0 5.686849986584204E-4 0.0 26-27 0.0 0.0 0.0 0.0013700138604043764 0.0 28-29 0.0 0.0 0.0 0.0037998497637630815 0.0 30-31 0.0 0.0 0.0 0.010572371111422452 0.0 32-33 0.0 0.0 0.0 0.026211208574529014 0.0 34-35 0.0 0.0 0.0 0.0516469376054329 0.0 36-37 0.0 0.0 0.0 0.09075695592225973 0.0 38-39 0.0 0.0 0.0 0.14912471623911033 0.0 40-41 0.0 0.0 0.0 0.21126647700160317 0.0 42-43 0.0 0.0 0.0 0.27488164889698374 0.0 44-45 0.0 0.0 0.0 0.3556090693883586 0.0 46-47 0.0 0.0 0.0 0.4521562725696859 0.0 48-49 0.0 0.0 0.0 0.5568977095953186 0.0 50-51 0.0 0.0 0.0 0.6690320516035106 0.0 52-53 0.0 0.0 0.0 0.7776508863472691 0.0 54-55 0.0 0.0 0.0 0.9037180108225925 0.0 56-57 0.0 0.0 0.0 1.0567459740979492 0.0 58-59 0.0 0.0 0.0 1.2314098166404468 0.0 60-61 0.0 0.0 0.0 1.4227723186890053 0.0 62-63 0.0 0.0 0.0 1.6113947930167551 0.0 64-65 0.0 0.0 0.0 1.8097366109579396 0.0 66-67 0.0 0.0 0.0 2.0415532958656084 0.0 68-69 0.0 0.0 0.0 2.3137724649961458 0.0 70-71 0.0 0.0 0.0 2.620319528591155 0.0 72-73 0.0 0.0 0.0 2.9249278933271023 0.0 74-75 0.0 0.0 0.0 3.234706121687216 0.0 76-77 0.0 0.0 0.0 3.580621696780261 0.0 78-79 0.0 0.0 0.0 3.9753149351673254 0.0 80-81 0.0 2.584931812083729E-5 0.0 4.412737096408134 0.0 82-83 5.169863624167458E-5 5.169863624167458E-5 0.0 4.8623601358019775 0.0 84-85 7.754795436251186E-5 5.169863624167458E-5 0.0 5.305908585437425 0.0 86-87 1.0339727248334916E-4 5.169863624167458E-5 0.0 5.777994682278276 0.0 88-89 1.0339727248334916E-4 5.169863624167458E-5 0.0 6.299168634230598 0.0 90-91 1.0339727248334916E-4 5.169863624167458E-5 0.0 6.87284255128634 0.0 92-93 1.0339727248334916E-4 5.169863624167458E-5 0.0 7.481774938258903 0.0 94-95 1.0339727248334916E-4 5.169863624167458E-5 0.0 8.093395654316035 0.0 96-97 1.0339727248334916E-4 5.169863624167458E-5 0.0 8.704034096284573 0.0 98-99 1.0339727248334916E-4 5.169863624167458E-5 0.0 9.36673306494848 0.0 100-101 1.0339727248334916E-4 5.169863624167458E-5 0.0 10.101784275032609 0.0 102-103 1.0339727248334916E-4 5.169863624167458E-5 0.0 10.868242406633556 0.0 104-105 1.0339727248334916E-4 5.169863624167458E-5 0.0 11.625110441211671 0.0 106-107 1.0339727248334916E-4 5.169863624167458E-5 0.0 12.385338887145497 0.0 108-109 1.0339727248334916E-4 5.169863624167458E-5 0.0 13.163687705082028 0.0 110-111 1.0339727248334916E-4 5.169863624167458E-5 0.0 14.010175325585086 0.0 112-113 1.0339727248334916E-4 5.169863624167458E-5 0.0 14.897117128947254 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4910 0.0 67.60473 1 GTATCAA 8910 0.0 50.43093 1 ATCAACG 11145 0.0 39.982944 3 TCAACGC 11205 0.0 39.718826 4 CAACGCA 11505 0.0 38.63142 5 TGGTATC 2215 0.0 38.14061 2 TATCAAC 11690 0.0 38.118896 2 AACGCAG 11990 0.0 37.16801 6 GTGGTAT 2535 0.0 34.08736 1 ACGCAGA 14030 0.0 31.682959 7 GTACATG 10490 0.0 31.18891 1 CGCAGAG 14250 0.0 31.026783 8 TACATGG 10595 0.0 30.77178 2 ACATGGG 10845 0.0 28.965254 3 GCAGAGT 15630 0.0 28.135088 9 GAGTACT 9950 0.0 26.791393 12-13 CATGGGA 7585 0.0 26.121334 4 CAGAGTA 15330 0.0 24.90071 10-11 CTAGACG 170 1.4005032E-5 24.49752 3 GTACTTT 10885 0.0 24.45957 14-15 >>END_MODULE