FastQCFastQC Report
Thu 26 May 2016
SRR1364103_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364103_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2397128
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN97690.407529343447659No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22800.047.7672351
GTATCAA38700.043.3662641
ATCAACG40900.039.437973
TCAACGC41100.039.3983154
CAACGCA42000.038.5556835
AACGCAG44650.036.400726
TATCAAC47850.034.2044682
TACATGG78650.030.3443452
GTACATG83100.030.1505281
ACGCAGA53850.030.0725487
ACATGGG75950.029.5448743
CGCAGAG53850.029.188068
TAAGGTG15200.025.850515
TGGTATC14050.024.1451912
GAGTACA54850.023.2194041
GTAAGGT14050.022.880634
GTGGTAT17250.022.4252931
GCAGAGT68000.022.0637159
TATGCCG1400.003146046321.2623255
GTGTAGC22700.021.2360171
Produced by FastQC (version 0.11.5)