##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364103_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2397128 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.10513289236119 31.0 31.0 34.0 28.0 34.0 2 31.138270880820716 33.0 31.0 34.0 28.0 34.0 3 31.238139557003215 33.0 31.0 34.0 28.0 34.0 4 34.729120013616296 37.0 35.0 37.0 32.0 37.0 5 34.76232683444522 37.0 35.0 37.0 32.0 37.0 6 34.804954095067096 37.0 35.0 37.0 32.0 37.0 7 34.73093927399788 37.0 35.0 37.0 32.0 37.0 8 34.66651885089156 37.0 35.0 37.0 32.0 37.0 9 36.21252515510227 39.0 37.0 39.0 32.0 39.0 10-11 36.129708968398845 39.0 37.0 39.0 31.0 39.0 12-13 36.03581994787095 39.0 37.0 39.0 31.0 39.0 14-15 37.31032906878565 40.0 38.0 41.0 31.0 41.0 16-17 37.31461878547996 40.0 38.0 41.0 31.0 41.0 18-19 37.32825677227082 40.0 38.0 41.0 31.0 41.0 20-21 37.34521977966967 40.0 38.0 41.0 31.0 41.0 22-23 37.31829964857947 40.0 38.0 41.0 31.0 41.0 24-25 37.32517183062398 40.0 37.5 41.0 31.0 41.0 26-27 37.28754618026238 40.0 37.0 41.0 31.0 41.0 28-29 37.24969380024763 40.0 37.0 41.0 31.0 41.0 30-31 37.19470925207165 40.0 37.0 41.0 31.0 41.0 32-33 37.09796014230362 40.0 37.0 41.0 30.0 41.0 34-35 37.072720772524455 40.0 37.0 41.0 30.0 41.0 36-37 37.01158094186043 40.0 37.0 41.0 30.0 41.0 38-39 36.926867693339695 40.0 37.0 41.0 30.0 41.0 40-41 36.852634485934836 40.0 37.0 41.0 30.0 41.0 42-43 36.744895766934434 40.0 36.5 41.0 30.0 41.0 44-45 36.65347344822638 40.0 36.0 41.0 30.0 41.0 46-47 36.55127448346521 40.0 36.0 41.0 30.0 41.0 48-49 36.44205983159848 40.0 36.0 41.0 30.0 41.0 50-51 36.322151341104856 40.0 36.0 41.0 29.5 41.0 52-53 36.18044655938273 40.0 35.0 41.0 29.0 41.0 54-55 36.007680649510576 39.0 35.0 41.0 29.0 41.0 56-57 35.82439131327155 39.0 35.0 41.0 28.0 41.0 58-59 35.623179070954905 39.0 35.0 41.0 28.0 41.0 60-61 35.38694199892538 38.5 35.0 41.0 28.0 41.0 62-63 35.130398960756374 38.0 34.0 40.0 28.0 41.0 64-65 34.850190519655186 37.5 34.0 40.0 27.5 41.0 66-67 34.55186831908851 37.0 34.0 40.0 26.5 41.0 68-69 34.22818180756305 36.5 34.0 39.5 26.0 41.0 70-71 33.87145701022223 36.0 34.0 39.0 26.0 41.0 72-73 33.53059390236983 35.5 33.0 39.0 26.0 40.5 74-75 33.25495447051638 35.0 33.0 38.0 26.0 40.0 76-77 33.024153904171996 35.0 33.0 37.0 26.0 39.5 78-79 32.71113870431617 35.0 33.0 37.0 26.0 39.0 80-81 32.49352871436152 35.0 33.0 36.5 26.0 39.0 82-83 32.36833848672245 35.0 33.0 36.0 26.0 37.5 84-85 32.13982044346401 35.0 33.0 36.0 26.0 37.0 86-87 31.93902995584716 35.0 33.0 35.5 25.5 37.0 88-89 31.737123132348376 35.0 33.0 35.0 25.0 36.0 90-91 31.58304062194426 35.0 33.0 35.0 25.0 36.0 92-93 31.448840237150456 35.0 33.0 35.0 24.5 36.0 94-95 31.333489283842997 35.0 33.0 35.0 24.0 36.0 96-97 31.236709303800215 35.0 33.0 35.0 24.0 35.5 98-99 31.147123975023444 35.0 33.0 35.0 23.5 35.0 100-101 31.063816992667896 35.0 33.0 35.0 23.0 35.0 102-103 29.94861663624137 34.0 30.0 35.0 19.5 35.0 104-105 30.77305008326631 35.0 32.0 35.0 20.0 35.0 106-107 30.86279914964908 35.0 32.5 35.0 20.0 35.0 108-109 30.83684997213332 35.0 33.0 35.0 19.5 35.0 110-111 30.74693424798342 35.0 33.0 35.0 19.0 35.0 112-113 30.65536675555081 35.0 32.5 35.0 18.0 35.0 114-115 30.53730130389366 35.0 32.0 35.0 16.0 35.0 116-117 30.424775815058688 35.0 32.0 35.0 8.5 35.0 118-119 30.280924506325903 35.0 32.0 35.0 2.0 35.0 120-121 30.123406217773937 35.0 32.0 35.0 2.0 35.0 122-123 29.94635726586148 35.0 32.0 35.0 2.0 35.0 124-125 29.100217218271197 34.5 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5015759331370724 1101 2 0.5298366925743068 1101 3 0.5528109595081467 1101 4 0.5954293326851214 1101 5 0.604731623978843 1101 6 0.5346907739854956 1101 7 0.5832976529695699 1101 8 0.5006827780395682 1101 9 0.5758735870345646 1101 10-11 0.6309735821098315 1101 12-13 0.6595696558463615 1101 14-15 0.7719927064414307 1101 16-17 0.7428038043889416 1101 18-19 0.7814925842076121 1101 20-21 0.7229244102126984 1101 22-23 0.7693375527947666 1101 24-25 0.8036093167982656 1101 26-27 0.758914650531004 1101 28-29 0.8253264014343173 1101 30-31 0.8487081189701726 1101 32-33 0.8645963358884714 1101 34-35 0.8441496535227486 1101 36-37 0.9011562784863401 1101 38-39 0.8709426444602499 1101 40-41 0.8337673011869242 1101 42-43 0.8415916251504285 1101 44-45 0.8700150940522988 1101 46-47 0.7959764802170426 1101 48-49 0.7397030685569135 1101 50-51 0.7124913170253961 1101 52-53 0.7650413450418228 1101 54-55 0.761764284128553 1101 56-57 0.7787682056768261 1101 58-59 0.777777332133887 1101 60-61 0.8552273236392054 1101 62-63 0.8757377530488313 1101 64-65 0.8088962652301888 1101 66-67 0.8779226933435851 1101 68-69 0.7921416404877135 1101 70-71 0.8408555461485676 1101 72-73 0.8585530667128864 1101 74-75 0.7842240318833618 1101 76-77 0.6789836604316903 1101 78-79 0.7772058150889762 1101 80-81 0.7008085950125817 1101 82-83 0.6691068789792922 1101 84-85 0.6294293448316708 1101 86-87 0.7188115770126373 1101 88-89 0.7064977095694829 1101 90-91 0.7133702640587991 1101 92-93 0.7451021280165193 1101 94-95 0.8040738761616701 1101 96-97 0.7524616933830472 1101 98-99 0.6959036169127657 1101 100-101 0.7040479777201583 1101 102-103 0.7171380911983078 1101 104-105 0.7090028217073936 1101 106-107 0.7038774321166201 1101 108-109 0.7553129408582926 1101 110-111 0.7845310533405438 1101 112-113 0.7429482690561215 1101 114-115 0.7253097386691927 1101 116-117 0.7346528437515794 1101 118-119 0.8258875944702027 1101 120-121 0.7909481059729302 1101 122-123 0.7381672629607827 1101 124-125 0.8194710581518798 1102 1 0.42550160346978316 1102 2 0.41465521865765353 1102 3 0.4057295760468662 1102 4 0.4168294020569121 1102 5 0.4197685484496816 1102 6 0.44237188337677935 1102 7 0.41628846792554697 1102 8 0.33722827520157495 1102 9 0.3397370384022338 1102 10-11 0.41464029925987944 1102 12-13 0.4630345793822954 1102 14-15 0.5502301935171587 1102 16-17 0.47194258562511493 1102 18-19 0.4646879397811645 1102 20-21 0.4664529444624108 1102 22-23 0.5371567886865591 1102 24-25 0.5612344078881364 1102 26-27 0.5946786368178891 1102 28-29 0.6006524961259885 1102 30-31 0.6261052485837766 1102 32-33 0.5729798553937684 1102 34-35 0.5292802282519489 1102 36-37 0.4649570928666975 1102 38-39 0.5001160037684684 1102 40-41 0.5513043471662229 1102 42-43 0.5358745237101914 1102 44-45 0.5533842523362651 1102 46-47 0.5225144828369821 1102 48-49 0.5904862880151143 1102 50-51 0.5060331052350762 1102 52-53 0.562399577019967 1102 54-55 0.47858214933859955 1102 56-57 0.4963085278227055 1102 58-59 0.4751864400760226 1102 60-61 0.45824974074680114 1102 62-63 0.5184474032421562 1102 64-65 0.4524403840798925 1102 66-67 0.47135361441750234 1102 68-69 0.48120718156860676 1102 70-71 0.4497116962265295 1102 72-73 0.39802933955336783 1102 74-75 0.32141134251885717 1102 76-77 0.4175972812891473 1102 78-79 0.34474685052681764 1102 80-81 0.41155145209855704 1102 82-83 0.40122033652606603 1102 84-85 0.41995788920016963 1102 86-87 0.36828580618870177 1102 88-89 0.36565927301921164 1102 90-91 0.401915641122347 1102 92-93 0.3797542331318624 1102 94-95 0.4397874177773673 1102 96-97 0.4882982861565388 1102 98-99 0.5438480172971438 1102 100-101 0.48271373026723197 1102 102-103 0.5093680027816205 1102 104-105 0.5209308299789264 1102 106-107 0.4891804907955013 1102 108-109 0.46895329772324246 1102 110-111 0.5278169439112723 1102 112-113 0.5012511948636416 1102 114-115 0.5930536310238104 1102 116-117 0.5409921680185832 1102 118-119 0.5617572392027022 1102 120-121 0.5301074781233908 1102 122-123 0.571888165417775 1102 124-125 0.6201832967656244 1103 1 0.42929669993408126 1103 2 0.5213185797180451 1103 3 0.49937140476420794 1103 4 0.48159589176270146 1103 5 0.4904100458828893 1103 6 0.4208304563404397 1103 7 0.5775601713168399 1103 8 0.5393290394381296 1103 9 0.5814370686205592 1103 10-11 0.6500489455907967 1103 12-13 0.6222222716583303 1103 14-15 0.7046483107476931 1103 16-17 0.5986277353899041 1103 18-19 0.6891985762044328 1103 20-21 0.6901036168019843 1103 22-23 0.7850834492452492 1103 24-25 0.8133005122962587 1103 26-27 0.8199698931275421 1103 28-29 0.8339936513340831 1103 30-31 0.7282496481093901 1103 32-33 0.4304135666128843 1103 34-35 0.6374709852076421 1103 36-37 0.6738780817220942 1103 38-39 0.6893004652002332 1103 40-41 0.7136207893499034 1103 42-43 0.6886794295128738 1103 44-45 0.613354598346902 1103 46-47 0.6502963832438553 1103 48-49 0.6726324126941918 1103 50-51 0.6405702757406146 1103 52-53 0.6128139715847567 1103 54-55 0.7543085004468821 1103 56-57 0.6912477748470707 1103 58-59 0.6432327873770731 1103 60-61 0.749370433721495 1103 62-63 0.8349286963001532 1103 64-65 0.7386267040309065 1103 66-67 0.7721293787927266 1103 68-69 0.6962206310933539 1103 70-71 0.7172796631331622 1103 72-73 0.7917230273808187 1103 74-75 0.8254010613759206 1103 76-77 0.7301498307420076 1103 78-79 0.7429802403175216 1103 80-81 0.696571524894253 1103 82-83 0.6932796142784241 1103 84-85 0.6375576219219639 1103 86-87 0.7013915977282679 1103 88-89 0.7224647019951789 1103 90-91 0.6186421455329345 1103 92-93 0.6849532556306457 1103 94-95 0.6801325272526633 1103 96-97 0.675688499552237 1103 98-99 0.7119127737381987 1103 100-101 0.6382500620716449 1103 102-103 0.7126701442790946 1103 104-105 0.7653716515474933 1103 106-107 0.7441092968630869 1103 108-109 0.8154477995401592 1103 110-111 0.765634333763721 1103 112-113 0.7985573588787886 1103 114-115 0.8790011948796135 1103 116-117 0.8925506303425088 1103 118-119 0.9580379739251441 1103 120-121 0.9733419704527684 1103 122-123 0.9850512069710611 1103 124-125 1.0788219384508473 1104 1 0.38886139875153347 1104 2 0.38841966084556034 1104 3 0.3791319424025765 1104 4 0.3367913089949539 1104 5 0.4081738129300163 1104 6 0.35063495399874256 1104 7 0.3378945762375096 1104 8 0.4178411995990743 1104 9 0.4661091735830709 1104 10-11 0.41151839387176636 1104 12-13 0.4819170097963479 1104 14-15 0.45604701526312397 1104 16-17 0.5130423070775976 1104 18-19 0.5455244052595276 1104 20-21 0.5818131647758022 1104 22-23 0.48841752283286155 1104 24-25 0.4676403618464988 1104 26-27 0.5088631892343969 1104 28-29 0.630187002583952 1104 30-31 0.6440353923954731 1104 32-33 -0.052029502093397184 1104 34-35 0.46100596459730525 1104 36-37 0.5721553873073759 1104 38-39 0.5909044814161604 1104 40-41 0.6490664996159694 1104 42-43 0.5549308016553312 1104 44-45 0.5554761750172545 1104 46-47 0.5828623856213753 1104 48-49 0.5248074737255877 1104 50-51 0.528762072060637 1104 52-53 0.5628236898962271 1104 54-55 0.5297700608633065 1104 56-57 0.5316623726613869 1104 58-59 0.5746864686378288 1104 60-61 0.6068629351832016 1104 62-63 0.6033074483897849 1104 64-65 0.6118700746975918 1104 66-67 0.5431718191141783 1104 68-69 0.5997626092507105 1104 70-71 0.5516983596601861 1104 72-73 0.6055877524436539 1104 74-75 0.5299607901974213 1104 76-77 0.5316591577479386 1104 78-79 0.5234267996987825 1104 80-81 0.5144232119812457 1104 82-83 0.42438272378181807 1104 84-85 0.5502139907411916 1104 86-87 0.5449816884549392 1104 88-89 0.4279815756361458 1104 90-91 0.4707581049796694 1104 92-93 0.5233139051384583 1104 94-95 0.5303055369484113 1104 96-97 0.5257533212835668 1104 98-99 0.4415381061103858 1104 100-101 0.4609879419913163 1104 102-103 0.5154441976937854 1104 104-105 0.4502515423223805 1104 106-107 0.46152874670769606 1104 108-109 0.49330481487450584 1104 110-111 0.4157350598066998 1104 112-113 0.38309910783441836 1104 114-115 0.5354279281137444 1104 116-117 0.4820236207599784 1104 118-119 0.4966645211852345 1104 120-121 0.43394258981204104 1104 122-123 0.521591701848859 1104 124-125 0.4881852445610342 1105 1 0.3182141783531556 1105 2 0.28225321498261735 1105 3 0.27008663037000247 1105 4 0.2710909985746994 1105 5 0.30487069279854495 1105 6 0.29812148238141134 1105 7 0.22255732930383232 1105 8 0.24887351205511976 1105 9 0.28247403199025456 1105 10-11 0.40381732974026363 1105 12-13 0.3785227838461438 1105 14-15 0.5562138807178982 1105 16-17 0.5259696205632025 1105 18-19 0.4337554371834784 1105 20-21 0.446686141119379 1105 22-23 0.38190238838863877 1105 24-25 0.44764758815025374 1105 26-27 0.416943051546113 1105 28-29 0.3884731642122716 1105 30-31 0.4524501511495984 1105 32-33 0.15332417885363014 1105 34-35 0.4794244919076789 1105 36-37 0.448072564542187 1105 38-39 0.4904101012271127 1105 40-41 0.5640348553987522 1105 42-43 0.4914743362502847 1105 44-45 0.4073101409759303 1105 46-47 0.39296891532497824 1105 48-49 0.40486326389859073 1105 50-51 0.3978298011752628 1105 52-53 0.4589430482315393 1105 54-55 0.47127993098409604 1105 56-57 0.516086968512866 1105 58-59 0.5242812517127433 1105 60-61 0.5275418192243322 1105 62-63 0.5298872310567617 1105 64-65 0.5130606665163313 1105 66-67 0.4269561489133551 1105 68-69 0.5326222829792044 1105 70-71 0.5563325183202892 1105 72-73 0.4793729758180234 1105 74-75 0.5374901324872283 1105 76-77 0.5170865003718674 1105 78-79 0.4908656966384797 1105 80-81 0.4912197837605916 1105 82-83 0.4018811405996843 1105 84-85 0.44574242391536245 1105 86-87 0.4846352532371938 1105 88-89 0.4115258056657183 1105 90-91 0.37962373069598954 1105 92-93 0.3809634915685436 1105 94-95 0.37931502827101937 1105 96-97 0.39535272763883 1105 98-99 0.4305939467898128 1105 100-101 0.46191609466058026 1105 102-103 0.44299117838090396 1105 104-105 0.5454835416385997 1105 106-107 0.5413797916079055 1105 108-109 0.4704281243662187 1105 110-111 0.46196786334059325 1105 112-113 0.4420944962093927 1105 114-115 0.4596144833036675 1105 116-117 0.4597386324347319 1105 118-119 0.4978786179166903 1105 120-121 0.533726771977058 1105 122-123 0.5104903898473694 1105 124-125 0.5081655859067595 1106 1 0.02794105247306078 1106 2 0.13766519936661936 1106 3 0.12837022648633933 1106 4 0.18054040219028877 1106 5 0.03966774338414325 1106 6 0.06887260936461104 1106 7 0.06425478323078693 1106 8 -0.03806149421531302 1106 9 0.07400925580002848 1106 10-11 0.13460684964388037 1106 12-13 0.0690827011442181 1106 14-15 0.08923002053467854 1106 16-17 0.04888889991021017 1106 18-19 0.07924665703686173 1106 20-21 0.18614164569663672 1106 22-23 0.13644552496082696 1106 24-25 0.15309400599217327 1106 26-27 0.1814946154567707 1106 28-29 0.1684783407858106 1106 30-31 0.1624459451835989 1106 32-33 0.2085293313930805 1106 34-35 0.2742936755805232 1106 36-37 0.2298051914375705 1106 38-39 0.23340339325056902 1106 40-41 0.30785162318321113 1106 42-43 0.1691168537905554 1106 44-45 0.18079899747699102 1106 46-47 0.21731129020133721 1106 48-49 0.25288214606683823 1106 50-51 0.22156808840730235 1106 52-53 0.23923709609635324 1106 54-55 0.21645816842242738 1106 56-57 0.3841216269052268 1106 58-59 0.3823749631002826 1106 60-61 0.2591783097539775 1106 62-63 0.23925197612591376 1106 64-65 0.2955076913731318 1106 66-67 0.27370453662638994 1106 68-69 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98-99 -2.271345438042676 2216 100-101 -2.329634825216072 2216 102-103 -2.5191857541440505 2216 104-105 -2.3135897871650357 2216 106-107 -2.2791707624087145 2216 108-109 -2.3387170698009285 2216 110-111 -2.282140810155461 2216 112-113 -2.2040234675181978 2216 114-115 -2.3100433069017363 2216 116-117 -2.3205075391013565 2216 118-119 -2.382995068907121 2216 120-121 -2.469874989377672 2216 122-123 -2.4948318936418943 2216 124-125 -2.7246837450920403 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 41664.0 3 7213.0 4 4042.0 5 3347.0 6 3612.0 7 4410.0 8 5022.0 9 5912.0 10 6517.0 11 7376.0 12 8360.0 13 8400.0 14 8535.0 15 7788.0 16 7092.0 17 6433.0 18 6497.0 19 6609.0 20 6744.0 21 7306.0 22 8632.0 23 10946.0 24 14140.0 25 18336.0 26 22441.0 27 26347.0 28 31864.0 29 39181.0 30 47464.0 31 57274.0 32 73212.0 33 96725.0 34 133457.0 35 196052.0 36 359546.0 37 719510.0 38 376822.0 39 2300.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.19363734741109 18.609539200984415 12.867049513282977 24.329773938321516 2 15.569624656194328 19.87830651687503 40.234703554960085 24.31736527197056 3 19.233067620561826 23.94507615600142 29.656210529668282 27.165645693768475 4 12.303586267413078 16.161691082585016 38.10576222377829 33.42896042622361 5 13.224906899612513 38.00176352208216 35.28020639243228 13.49312318587305 6 32.47824900108309 36.721439195113454 17.5355255483298 13.264786255473654 7 28.294595745965513 32.31906432709305 21.343549680159132 18.042790246782303 8 25.715879189424356 35.6498128715051 20.2371761943201 18.397131744750443 9 25.738918786764568 16.210859771391334 20.293852341630604 37.75636910021349 10-11 24.435705798195016 26.47196742853811 28.670360749828138 20.421966023438735 12-13 25.04255503000874 24.06248898896464 28.24802472512261 22.64693125590401 14-15 21.9711104764469 26.045004682395994 25.73174756748165 26.25213727367546 16-17 22.11724491783596 29.08079147375261 26.716988395696617 22.08497521271481 18-19 21.976057711120202 28.176614380765308 28.13293386879532 21.71439403931917 20-21 22.531945840120652 27.491393873713065 28.189320518649126 21.787339767517157 22-23 22.26135841479755 27.626157946030084 27.763545905425662 22.348937733746705 24-25 22.05550459658319 27.71792719871189 28.01915134647026 22.207416858234655 26-27 21.76477607833457 27.95472805774467 27.779734040132436 22.500761823788324 28-29 21.92570611523757 28.336031613598156 27.643110725386222 22.095151545778048 30-31 22.078394844525935 27.86320513567887 27.620546238346734 22.437853781448464 32-33 21.718806192365964 27.987061666780257 27.662890364926003 22.63124177592778 34-35 22.4657264803489 28.040392195841257 27.476481711912452 22.017399611897396 36-37 22.541392994409392 27.530831749880218 27.448022706151765 22.479752549558622 38-39 21.936414961747484 28.0394480507916 27.521801859637552 22.50233512782337 40-41 22.505896960231713 27.740373675986284 27.64334463767375 22.110384726108247 42-43 22.07096082153471 27.81742490265052 27.5039242586132 22.60769001720157 44-45 22.160966904525807 28.04999233027913 27.747080267191283 22.041960498003775 46-47 22.457649584695066 28.06857796699146 27.11111856230203 22.36265388601145 48-49 22.073891091825317 28.045000405562003 27.37384244534397 22.50726605726871 50-51 22.215614211776817 28.242916052142654 27.387108847343644 22.15436088873689 52-53 22.50697975885648 27.921675821084307 27.345378145449313 22.2259662746099 54-55 22.088331366144217 28.109857786161058 27.578035734603173 22.22377511309155 56-57 22.1110072449771 28.3492254105075 27.29105483531305 22.24871250920235 58-59 22.021202165686002 27.92003330793052 27.4514257600467 22.607338766336778 60-61 21.829593879423093 28.07651643059098 27.674619946855213 22.419269743130716 62-63 21.86530714630442 28.024537084867408 27.86539641931407 22.2447593495141 64-65 21.69264979267077 28.16793971515204 27.552186465750705 22.587224026426487 66-67 21.661830334220404 28.287745503426372 27.36592223613346 22.68450192621977 68-69 22.18967665236944 27.718122155785014 27.612569711562646 22.479631480282904 70-71 21.843323516269415 27.779063245868407 27.531813239371207 22.845799998490975 72-73 21.858969951755792 27.76022862758053 27.55753419603884 22.82326722462484 74-75 21.747688069468055 28.19803870239722 27.396253669651994 22.65801955848273 76-77 21.911615130098756 27.889729859785632 27.52421386290677 22.674441147208842 78-79 21.959957139156046 27.95362107894964 27.480701301336385 22.605720480557927 80-81 22.01074591578698 27.868118877872362 27.40219005410685 22.718945152233808 82-83 21.902174277560356 27.970327742956698 27.531221599578792 22.596276379904157 84-85 21.88137683455819 27.995781333574783 27.46945407170056 22.653387760166467 86-87 21.923655348803287 28.00502643573229 27.403358893044054 22.667959322420366 88-89 21.857256389900233 28.009785733267723 27.314921545631126 22.818036331200922 90-91 21.821046962651586 27.98976968162797 27.399357514212852 22.7898258415076 92-93 21.8930882637069 27.637242916410138 27.717857557470676 22.751811262412286 94-95 21.72087109287254 27.492045770763973 27.822976071935063 22.96410706442842 96-97 21.978551991223682 27.196384377951592 27.753012669175863 23.072050961648863 98-99 21.55218346354974 27.466502018172772 27.620943757571286 23.3603707607062 100-101 21.589449716133043 27.343043814448105 27.844868823055847 23.222637646363008 102-103 21.510513269354846 26.989764517090304 27.9568341014954 23.542888112059448 104-105 21.338042548379228 27.17609399721678 27.82199502202602 23.66386843237798 106-107 21.479955510907303 27.29337984960743 27.487909684880098 23.738754954605167 108-109 21.432838134175025 27.174956173513092 27.617095785745555 23.77510990656633 110-111 21.4676616550631 27.224493960161215 27.7429306515769 23.564913733198782 112-113 21.43186496069384 27.37709296783756 27.78239943017854 23.408642641290058 114-115 21.307983290271416 27.502657242341762 27.600253553498654 23.58910591388817 116-117 21.233638540568712 27.479040742149603 27.661947237801904 23.625373479479787 118-119 21.116951120920056 27.54390030976127 27.483423308414682 23.855725260903988 120-121 21.092184484217114 27.736354608867984 27.510991056912104 23.660469850002798 122-123 21.111756280239685 27.653289461832593 27.44432169473362 23.790632563194105 124-125 21.046097715895552 27.54567287146235 27.53844548835323 23.869783924288868 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 9780.0 1 5181.5 2 550.5 3 477.5 4 413.5 5 390.0 6 342.5 7 298.0 8 304.5 9 299.0 10 299.0 11 289.0 12 287.5 13 327.0 14 359.5 15 420.0 16 508.5 17 570.0 18 708.5 19 952.5 20 1299.0 21 1931.0 22 2757.0 23 3960.5 24 5596.5 25 7958.5 26 12196.5 27 17635.0 28 22082.5 29 26891.0 30 32864.0 31 39498.0 32 48938.5 33 54557.0 34 56513.5 35 60841.0 36 66215.0 37 71274.0 38 73171.0 39 74178.0 40 77544.5 41 81869.5 42 83159.5 43 83839.0 44 85640.5 45 89064.5 46 89629.5 47 87800.0 48 86143.0 49 83763.0 50 82406.5 51 79863.0 52 77842.0 53 77401.0 54 76939.0 55 71110.5 56 63860.0 57 58429.0 58 54098.0 59 47899.5 60 37930.0 61 31158.5 62 26390.5 63 19014.5 64 12585.0 65 9232.0 66 6581.5 67 4840.5 68 3807.5 69 2857.0 70 2043.5 71 1335.0 72 877.5 73 643.0 74 438.5 75 295.5 76 206.5 77 107.0 78 56.0 79 39.0 80 26.5 81 24.5 82 19.0 83 14.0 84 10.0 85 6.5 86 5.0 87 5.0 88 4.5 89 5.5 90 8.0 91 7.5 92 6.0 93 6.0 94 5.0 95 2.5 96 1.5 97 2.5 98 1.5 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4648062181076688 2 0.45758924846733257 3 0.4522912418527505 4 0.449496230489152 5 0.4586321631552424 6 0.5129054435140719 7 0.5086086349998832 8 0.5174108349658425 9 0.5226671249929082 10-11 0.5404383912748922 12-13 0.5488234253656876 14-15 0.5283405808951379 16-17 0.523063432574314 18-19 0.5326999642906011 20-21 0.5331379884595232 22-23 0.5424616457694373 24-25 0.5363501656982855 26-27 0.5442554590326424 28-29 0.5249823956000681 30-31 0.530259543920892 32-33 0.5314901832526256 34-35 0.5300926775708265 36-37 0.4805333716013496 38-39 0.4484115992137257 40-41 0.4475772674633978 42-43 0.4592370536742302 44-45 0.46397188635734093 46-47 0.4684981361028698 48-49 0.48180572752059964 50-51 0.4645142019950541 52-53 0.46395102806358274 54-55 0.45460651245991035 56-57 0.43839961820979106 58-59 0.45510711151010713 60-61 0.45124832716484065 62-63 0.46674603942718124 64-65 0.46674603942718124 66-67 0.4741924502988576 68-69 0.4832449497899152 70-71 0.4791567242133086 72-73 0.4907539355428663 74-75 0.4803039303700095 76-77 0.4780512346441241 78-79 0.48950243791737447 80-81 0.48268177585844396 82-83 0.4839124151901776 84-85 0.48140941993919395 86-87 0.47314953561094775 88-89 0.4759862635620626 90-91 0.4623449394442015 92-93 0.4624283726192343 94-95 0.4578812645799474 96-97 0.4466177859505208 98-99 0.4096568894109952 100-101 0.4064029955847164 102-103 0.3964118728745399 104-105 0.40091726432631053 106-107 0.3994989003507531 108-109 0.4118678685493641 110-111 0.4352500158523033 112-113 0.43449911727700813 114-115 0.4278036049806268 116-117 0.422004999315848 118-119 0.4629289716694311 120-121 0.45819413898632033 122-123 0.4237153794040201 124-125 0.432684445720045 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2397128.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.58546842987266 #Duplication Level Percentage of deduplicated Percentage of total 1 85.11664891691831 60.93115183898611 2 9.476101052175542 13.567022654175908 3 2.5760813436939345 5.532299691053582 4 1.0613675528657038 3.039139737926362 5 0.5222258622572755 1.8691891487940613 6 0.2984904703557083 1.2820548085359842 7 0.18521948766462656 0.9281316650769321 8 0.12711940067788416 0.7279921475240808 9 0.0944500897048756 0.6085128523290313 >10 0.4821194048593807 6.318727485396301 >50 0.04127102837987373 2.0165819295703993 >100 0.018653319509832917 2.2437631416118595 >500 1.2603546853441746E-4 0.054043625242350346 >1k 0.0 0.0 >5k 1.2603546853441746E-4 0.8813892737771629 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 9769 0.407529343447659 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.1716587516394616E-5 2 0.0 0.0 0.0 0.0 4.1716587516394616E-5 3 0.0 0.0 0.0 0.0 4.1716587516394616E-5 4 0.0 0.0 0.0 0.0 4.1716587516394616E-5 5 0.0 0.0 0.0 0.0 4.1716587516394616E-5 6 0.0 0.0 0.0 0.0 8.343317503278923E-5 7 4.1716587516394616E-5 0.0 0.0 0.0 8.343317503278923E-5 8 4.1716587516394616E-5 0.0 0.0 0.0 8.343317503278923E-5 9 4.1716587516394616E-5 0.0 0.0 0.0 8.343317503278923E-5 10-11 4.1716587516394616E-5 0.0 0.0 4.1716587516394616E-5 1.6686635006557847E-4 12-13 4.1716587516394616E-5 0.0 0.0 6.257488127459193E-5 3.545909938893543E-4 14-15 4.1716587516394616E-5 0.0 0.0 8.343317503278923E-5 4.171658751639462E-4 16-17 6.257488127459193E-5 0.0 0.0 8.343317503278923E-5 5.840322252295247E-4 18-19 1.6686635006557847E-4 0.0 0.0 1.0429146879098655E-4 6.257488127459193E-4 20-21 2.085829375819731E-4 0.0 0.0 2.085829375819731E-4 6.883236940205112E-4 22-23 2.085829375819731E-4 0.0 0.0 2.085829375819731E-4 7.091819877787086E-4 24-25 2.085829375819731E-4 0.0 0.0 2.9201611261476233E-4 7.091819877787086E-4 26-27 2.085829375819731E-4 0.0 0.0 6.466071065041166E-4 7.091819877787086E-4 28-29 2.085829375819731E-4 0.0 0.0 0.0019398213195123497 7.091819877787086E-4 30-31 2.085829375819731E-4 0.0 0.0 0.0072169696403362685 7.300402815369059E-4 32-33 2.085829375819731E-4 0.0 0.0 0.020441127883033365 7.508985752951032E-4 34-35 2.085829375819731E-4 0.0 0.0 0.03967247472809128 7.926151628114978E-4 36-37 2.085829375819731E-4 0.0 0.0 0.074422392129248 7.926151628114978E-4 38-39 2.502995250983677E-4 0.0 0.0 0.12191672701666327 8.343317503278924E-4 40-41 2.502995250983677E-4 0.0 0.0 0.16770068181590636 8.343317503278924E-4 42-43 2.502995250983677E-4 0.0 0.0 0.2161336399224405 8.343317503278924E-4 44-45 2.502995250983677E-4 0.0 0.0 0.2799183022350079 8.551900440860896E-4 46-47 3.545909938893543E-4 0.0 0.0 0.3629551696863914 8.76048337844287E-4 48-49 3.754492876475516E-4 0.0 0.0 0.4522495252652341 9.594815128770762E-4 50-51 3.754492876475516E-4 0.0 0.0 0.5461535637646384 0.0010429146879098654 52-53 3.754492876475516E-4 0.0 0.0 0.6378466231256736 0.0011054895691844575 54-55 3.754492876475516E-4 0.0 0.0 0.7425761160855824 0.0011680644504590493 56-57 3.754492876475516E-4 0.0 0.0 0.8649517255649259 0.0011889227442172467 58-59 3.754492876475516E-4 0.0 0.0 1.005870358195307 0.001209781037975444 60-61 3.754492876475516E-4 0.0 0.0 1.1656657466768565 0.001209781037975444 62-63 3.754492876475516E-4 0.0 0.0 1.3195165214373201 0.0013349308005246277 64-65 3.754492876475516E-4 0.0 0.0 1.4839633094269478 0.0013349308005246277 66-67 3.754492876475516E-4 0.0 0.0 1.685078977843486 0.0013349308005246277 68-69 3.754492876475516E-4 0.0 0.0 1.9154588324027753 0.0013349308005246277 70-71 3.963075814057489E-4 0.0 0.0 2.170388898715463 0.0013766473880410224 72-73 4.171658751639462E-4 0.0 0.0 2.4304918218801834 0.0014600805630738119 74-75 4.171658751639462E-4 0.0 0.0 2.694432671096412 0.0015017971505902063 76-77 4.171658751639462E-4 0.0 0.0 2.9899321187687935 0.0015226554443484035 78-79 4.171658751639462E-4 0.0 0.0 3.3261260975634173 0.0015435137381066008 80-81 4.588824626803408E-4 0.0 0.0 3.700073588060379 0.0015643720318647982 82-83 4.588824626803408E-4 0.0 0.0 4.091291745789128 0.00162694691313939 84-85 4.588824626803408E-4 0.0 0.0 4.483365093561963 0.00162694691313939 86-87 4.588824626803408E-4 0.0 0.0 4.89819483982499 0.0017312383819303766 88-89 4.797407564385381E-4 4.1716587516394616E-5 0.0 5.350423506796466 0.0018355298507213632 90-91 5.005990501967354E-4 4.1716587516394616E-5 0.0 5.857947510520923 0.0018355298507213632 92-93 5.4231563771313E-4 4.1716587516394616E-5 0.0 6.382992481002265 0.001960679613270547 94-95 5.4231563771313E-4 4.1716587516394616E-5 0.0 6.908412900771256 0.002002396200786942 96-97 5.4231563771313E-4 8.343317503278923E-5 0.0 7.456401994386615 0.0022735540196435066 98-99 5.631739314713273E-4 8.343317503278923E-5 0.0 8.033279824857079 0.0024612786634672823 100-101 6.04890518987722E-4 8.343317503278923E-5 0.0 8.678322559329331 0.0024612786634672823 102-103 6.257488127459193E-4 1.0429146879098655E-4 0.0 9.349229578061747 0.0024612786634672823 104-105 6.674654002623139E-4 1.2514976254918386E-4 0.0 10.02827133135986 0.0024612786634672823 106-107 6.883236940205112E-4 1.2514976254918386E-4 0.0 10.69775998611672 0.0024612786634672823 108-109 7.091819877787086E-4 1.2514976254918386E-4 0.0 11.40112250993689 0.0024612786634672823 110-111 7.091819877787086E-4 1.2514976254918386E-4 0.0 12.159759512216286 0.0024612786634672823 112-113 7.508985752951032E-4 1.2514976254918386E-4 0.0 12.95740152382351 0.0024612786634672823 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2280 0.0 47.767235 1 GTATCAA 3870 0.0 43.366264 1 ATCAACG 4090 0.0 39.43797 3 TCAACGC 4110 0.0 39.398315 4 CAACGCA 4200 0.0 38.555683 5 AACGCAG 4465 0.0 36.40072 6 TATCAAC 4785 0.0 34.204468 2 TACATGG 7865 0.0 30.344345 2 GTACATG 8310 0.0 30.150528 1 ACGCAGA 5385 0.0 30.072548 7 ACATGGG 7595 0.0 29.544874 3 CGCAGAG 5385 0.0 29.18806 8 TAAGGTG 1520 0.0 25.85051 5 TGGTATC 1405 0.0 24.145191 2 GAGTACA 5485 0.0 23.219404 1 GTAAGGT 1405 0.0 22.88063 4 GTGGTAT 1725 0.0 22.425293 1 GCAGAGT 6800 0.0 22.063715 9 TATGCCG 140 0.0031460463 21.262325 5 GTGTAGC 2270 0.0 21.236017 1 >>END_MODULE