FastQCFastQC Report
Thu 26 May 2016
SRR1364103_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364103_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2397128
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56430.23540670335501485No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55170.2301504133279491No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29180.12172900237283948No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42900.071.827771
GTATCAA77800.052.0940861
ATCAACG95200.042.3088383
TCAACGC95300.042.264444
CAACGCA97000.041.5237245
TATCAAC98800.040.768922
AACGCAG101550.039.781246
TGGTATC18000.035.367862
ACGCAGA116400.034.452647
CGCAGAG116400.034.2481738
GTGGTAT20950.031.5789761
GCAGAGT132350.029.7611459
ACATGGG75550.028.0346343
TACATGG79200.027.8705942
GTACATG81700.027.64881
GAGTACT94800.027.30184412-13
CAGAGTA127150.026.6263410-11
GTACTTT99850.025.62121614-15
AGAGTAC122700.024.5612510-11
AGTACTT100300.023.04629912-13
Produced by FastQC (version 0.11.5)