FastQCFastQC Report
Thu 26 May 2016
SRR1364102_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364102_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1464172
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN58620.40036279890613946No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25650.060.550551
GTATCAA44050.045.524891
ATGGGCG1950.042.7446755
GTACATG107450.039.4310651
TCAACGC50750.038.0086634
ATCAACG51500.037.5578463
CAACGCA52500.036.742975
TACATGG113200.036.6922152
ACATGGG112350.035.6505743
AACGCAG53800.035.523146
TATCAAC56850.034.5420762
CATGGGA82250.032.7895854
ACGCAGA65450.029.1101077
CGCAGAG65600.029.0435438
ATGGGAT32450.027.5210595
ATGGGAG29950.027.4328425
GAGTACA77550.026.7798751
CATGGGG32750.026.3590874
AGTACAT76050.026.2126262
GTCGGGA4350.025.9913772
Produced by FastQC (version 0.11.5)