FastQCFastQC Report
Thu 26 May 2016
SRR1364102_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364102_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1464172
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106290.725939302213128No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100390.6856434899724896No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58910.40234344052474713No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29250.1997716115319785No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20190.13789363544720157No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48800.072.945841
GTATCAA92300.056.0743261
TGGTATC19650.046.630362
GTGGTAT21050.043.9061781
ATCAACG119150.043.191973
TCAACGC120550.042.691824
TATCAAC120950.042.5520822
CAACGCA122050.042.1671375
AACGCAG127050.041.0241936
ACGCAGA146050.035.608217
GTACATG95350.035.2073821
CGCAGAG147500.035.0564588
TACATGG99050.033.2185942
ACATGGG96600.033.0733453
GCAGAGT157800.032.5796979
ATGGGAG25050.029.689175
CATGGGA70300.028.6906344
GAGTACT110550.027.71829612-13
CAGAGTA156350.027.53426610-11
CATGGGG29700.027.2444634
Produced by FastQC (version 0.11.5)