##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364102_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1464172 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.06898847949558 33.0 31.0 34.0 30.0 34.0 2 32.069031507227294 33.0 31.0 34.0 30.0 34.0 3 32.19747884811347 34.0 31.0 34.0 30.0 34.0 4 35.858833525023016 37.0 35.0 37.0 35.0 37.0 5 35.80480571954661 37.0 35.0 37.0 35.0 37.0 6 35.85540018522414 37.0 35.0 37.0 35.0 37.0 7 35.777234505235725 37.0 35.0 37.0 33.0 37.0 8 35.76182647940269 37.0 35.0 37.0 33.0 37.0 9 37.401497911447564 39.0 37.0 39.0 34.0 39.0 10-11 37.33198285447338 39.0 37.0 39.0 34.0 39.0 12-13 37.28043358293971 39.0 37.0 39.0 33.0 39.0 14-15 38.5215647478575 40.0 38.0 41.0 34.0 41.0 16-17 38.510297970457025 40.0 38.0 41.0 33.5 41.0 18-19 38.691301636692955 40.0 38.0 41.0 34.0 41.0 20-21 38.77117613231232 40.0 38.0 41.0 34.0 41.0 22-23 38.72912574478954 40.0 38.0 41.0 34.0 41.0 24-25 38.69462023587393 40.0 38.0 41.0 34.0 41.0 26-27 38.55789688643138 40.0 38.0 41.0 34.0 41.0 28-29 38.462421423166134 40.0 38.0 41.0 34.0 41.0 30-31 38.265043656073196 40.0 37.5 41.0 34.0 41.0 32-33 38.0504076706835 40.0 37.0 41.0 33.0 41.0 34-35 37.891410298790035 40.0 37.0 41.0 33.0 41.0 36-37 37.73826982075876 40.0 36.5 41.0 33.0 41.0 38-39 37.604318003622524 40.0 36.0 41.0 33.0 41.0 40-41 37.43403780430168 40.0 36.0 41.0 33.0 41.0 42-43 37.232218619123984 40.0 36.0 41.0 32.0 41.0 44-45 36.96234253899132 40.0 36.0 41.0 31.0 41.0 46-47 36.527568482391416 40.0 35.5 41.0 30.5 41.0 48-49 36.106212931267635 40.0 35.0 41.0 29.5 41.0 50-51 35.87442151605139 40.0 35.0 41.0 28.0 41.0 52-53 35.71616107943602 39.0 35.0 41.0 28.0 41.0 54-55 35.54557422215423 39.0 35.0 41.0 27.0 41.0 56-57 35.360822020910106 39.0 35.0 41.0 27.0 41.0 58-59 35.149576347587576 39.0 35.0 41.0 26.5 41.0 60-61 34.91556729673836 38.0 35.0 40.5 26.0 41.0 62-63 34.66642716839279 38.0 34.0 40.0 26.0 41.0 64-65 34.45025003892985 37.0 34.0 40.0 26.0 41.0 66-67 34.24678248183957 37.0 34.0 40.0 26.0 41.0 68-69 33.93904404673768 36.5 34.0 39.0 26.0 41.0 70-71 33.62395265037168 36.0 34.0 39.0 26.0 41.0 72-73 33.297523788188826 35.5 34.0 39.0 25.0 40.5 74-75 33.02192365377838 35.0 34.0 37.5 25.5 40.0 76-77 32.81779872856468 35.0 34.0 37.0 26.0 39.0 78-79 32.53867373505298 35.0 34.0 37.0 25.0 39.0 80-81 32.265079854006224 35.0 33.0 36.0 25.0 39.0 82-83 32.00847646314777 35.0 33.0 36.0 25.0 37.0 84-85 31.796251738183766 35.0 33.0 35.5 24.0 37.0 86-87 31.61515860158506 35.0 33.0 35.0 24.0 36.5 88-89 31.465094606371384 35.0 33.0 35.0 24.0 36.0 90-91 31.33054279142068 35.0 33.0 35.0 23.0 36.0 92-93 31.22510743273331 35.0 33.0 35.0 23.0 36.0 94-95 31.137205191739767 35.0 33.0 35.0 23.0 35.5 96-97 31.067486265274844 35.0 33.0 35.0 20.5 35.0 98-99 31.00855295689304 35.0 33.0 35.0 20.0 35.0 100-101 30.94525404119188 35.0 33.0 35.0 20.0 35.0 102-103 29.774411407949337 33.5 30.0 34.5 18.5 35.0 104-105 30.685220042454027 35.0 32.5 35.0 19.0 35.0 106-107 30.82709066967542 35.0 33.0 35.0 19.0 35.0 108-109 30.817501290831952 35.0 33.0 35.0 19.0 35.0 110-111 30.77310350150119 35.0 33.0 35.0 18.5 35.0 112-113 30.7050124575528 35.0 33.0 35.0 17.5 35.0 114-115 30.63026987266523 35.0 33.0 35.0 15.5 35.0 116-117 30.56442480801436 35.0 33.0 35.0 8.5 35.0 118-119 30.471764246277075 35.0 33.0 35.0 2.0 35.0 120-121 30.344375182697114 35.0 33.0 35.0 2.0 35.0 122-123 30.19536024456143 35.0 33.0 35.0 2.0 35.0 124-125 29.36013562614228 34.5 31.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.16472994639708105 1101 2 0.20961762061794786 1101 3 0.13272934793806712 1101 4 0.13778317661010675 1101 5 0.16703230384896273 1101 6 0.21879710124421337 1101 7 0.2694526148541527 1101 8 0.19003867135445773 1101 9 0.31498862600419386 1101 10-11 0.2793915122927473 1101 12-13 0.31617193029542534 1101 14-15 0.43448044983515643 1101 16-17 0.3994901259829575 1101 18-19 0.31314292954782275 1101 20-21 0.32815798263926155 1101 22-23 0.3739382837295011 1101 24-25 0.35130648451743696 1101 26-27 0.3836804829122116 1101 28-29 0.37092807374507686 1101 30-31 0.44572949770284254 1101 32-33 0.41167062763731366 1101 34-35 0.4801525631129664 1101 36-37 0.5408203110023209 1101 38-39 0.5235435221361513 1101 40-41 0.5713993274219291 1101 42-43 0.5258666645961014 1101 44-45 0.46629137138054233 1101 46-47 0.4171996436005969 1101 48-49 0.6911178691238931 1101 50-51 0.6385005226084388 1101 52-53 0.5837765214766435 1101 54-55 0.6404701594252415 1101 56-57 0.5733986428424558 1101 58-59 0.5829108703953452 1101 60-61 0.5939793056792695 1101 62-63 0.6080012843338807 1101 64-65 0.533403704772347 1101 66-67 0.6074908048116328 1101 68-69 0.6575913204397779 1101 70-71 0.6072665218591524 1101 72-73 0.5926717577188043 1101 74-75 0.646081407667225 1101 76-77 0.6725461264212242 1101 78-79 0.6544725723566174 1101 80-81 0.5957883874389438 1101 82-83 0.6282064352475061 1101 84-85 0.5673714487514445 1101 86-87 0.5890431055553158 1101 88-89 0.56729198358925 1101 90-91 0.5722453580341487 1101 92-93 0.6554716574734663 1101 94-95 0.5294186254568736 1101 96-97 0.6355194040097452 1101 98-99 0.6882679567677457 1101 100-101 0.6822739861215794 1101 102-103 0.6248084591524652 1101 104-105 0.6589165074726075 1101 106-107 0.6004380882498879 1101 108-109 0.604873754146876 1101 110-111 0.5699055861255928 1101 112-113 0.6814749982696497 1101 114-115 0.6737046011811927 1101 116-117 0.6646420794558416 1101 118-119 0.6818010680639937 1101 120-121 0.6133204797143677 1101 122-123 0.6342825873827351 1101 124-125 0.6742085214773894 1102 1 0.25515614424397626 1102 2 0.35040220579662673 1102 3 0.18942077591129447 1102 4 0.1845074843510588 1102 5 0.20167228214275923 1102 6 0.21089100192979515 1102 7 0.20989729288239545 1102 8 0.2755698644050568 1102 9 0.26512653302217615 1102 10-11 0.2571470355168941 1102 12-13 0.2838501999522407 1102 14-15 0.34314840263698443 1102 16-17 0.392870268654228 1102 18-19 0.33862603139819925 1102 20-21 0.39426800060670786 1102 22-23 0.4025614140799405 1102 24-25 0.38474738746123194 1102 26-27 0.4269026360562975 1102 28-29 0.3960942351443606 1102 30-31 0.47619904385950207 1102 32-33 0.5204168808606653 1102 34-35 0.400089794589519 1102 36-37 0.43785173158347845 1102 38-39 0.363788229647227 1102 40-41 0.37633321537434483 1102 42-43 0.3138852045976819 1102 44-45 0.29518967483580383 1102 46-47 0.32853525864880595 1102 48-49 0.30318510707385116 1102 50-51 0.18558150853145605 1102 52-53 0.2858265397431836 1102 54-55 0.39419396937633877 1102 56-57 0.33257677683552345 1102 58-59 0.35715291590572207 1102 60-61 0.2634719445850706 1102 62-63 0.2532940723629977 1102 64-65 0.13895181840516813 1102 66-67 0.15243097388926685 1102 68-69 0.12513755828077677 1102 70-71 0.14212356216732047 1102 72-73 0.22173168264440335 1102 74-75 0.20382161263700738 1102 76-77 0.2727960895676844 1102 78-79 0.14076743486620558 1102 80-81 0.20513668865093848 1102 82-83 0.15458929760327322 1102 84-85 0.14241423090535932 1102 86-87 0.13542643792725428 1102 88-89 0.17110103738260207 1102 90-91 0.13403240225198587 1102 92-93 0.22131451397539337 1102 94-95 0.17486107090828895 1102 96-97 0.1833366061743824 1102 98-99 0.19058641836208423 1102 100-101 0.20183595241287122 1102 102-103 0.28557001353868117 1102 104-105 0.24544253248174286 1102 106-107 0.22063276040292834 1102 108-109 0.16801561998289571 1102 110-111 0.17247881784997077 1102 112-113 0.23190367438364135 1102 114-115 0.20444088151592865 1102 116-117 0.17420904501006262 1102 118-119 0.17690296543382544 1102 120-121 0.12878999382446565 1102 122-123 0.10009168817770586 1102 124-125 0.36028696290376416 1103 1 0.18889647210575333 1103 2 0.22883012613593223 1103 3 0.15193814041627718 1103 4 0.13243928724218534 1103 5 0.11886138850213968 1103 6 0.1464336689102339 1103 7 0.1411382111477124 1103 8 0.053739169314347635 1103 9 0.175100264733679 1103 10-11 0.18920507643964157 1103 12-13 0.13877196577557527 1103 14-15 0.10098779959112392 1103 16-17 0.23041974737666493 1103 18-19 0.27583863768639816 1103 20-21 0.20465217173200756 1103 22-23 0.3548670800032525 1103 24-25 0.2765027599259682 1103 26-27 0.31218982120726224 1103 28-29 0.21086202289293965 1103 30-31 0.23337291386447134 1103 32-33 0.24924370232304227 1103 34-35 0.3281143105519959 1103 36-37 0.28825872611192693 1103 38-39 0.39286721256765134 1103 40-41 0.35195805739729735 1103 42-43 0.38440314933757236 1103 44-45 0.17656432106060294 1103 46-47 0.20755295996396939 1103 48-49 0.2858373077122778 1103 50-51 0.29481083371992156 1103 52-53 0.3347371673805455 1103 54-55 0.3261128659837027 1103 56-57 0.33644791488038095 1103 58-59 0.36043769710777696 1103 60-61 0.38515691687450015 1103 62-63 0.36683749389784737 1103 64-65 0.32597288076630804 1103 66-67 0.3391931510402131 1103 68-69 0.4185815056379454 1103 70-71 0.3535668902752107 1103 72-73 0.4196304893444207 1103 74-75 0.31568336980247125 1103 76-77 0.30157281492606813 1103 78-79 0.29209969239919076 1103 80-81 0.2961081214202679 1103 82-83 0.2701353181588644 1103 84-85 0.19986900433358912 1103 86-87 0.20963995607246133 1103 88-89 0.1326066224548761 1103 90-91 0.10559093064676617 1103 92-93 0.11730367130247998 1103 94-95 0.15521785727019832 1103 96-97 0.17377196497579206 1103 98-99 0.17266782639878997 1103 100-101 0.1751639274968042 1103 102-103 0.26784919119888784 1103 104-105 0.2426833038206233 1103 106-107 0.1315952364703108 1103 108-109 0.18697525215215194 1103 110-111 0.1332783051846249 1103 112-113 0.15998619308459539 1103 114-115 0.12571954410351793 1103 116-117 0.13085651162891665 1103 118-119 0.19587287000220144 1103 120-121 0.07485771944059394 1103 122-123 -0.008941527572972063 1103 124-125 0.27476093928513023 1104 1 0.1753373351025047 1104 2 0.20707761709593342 1104 3 0.12096919296956088 1104 4 0.07837475583657039 1104 5 0.09079519728040708 1104 6 0.0901680937193774 1104 7 0.09659109916240283 1104 8 0.17682618127248162 1104 9 0.14515188509167842 1104 10-11 0.13058325812223615 1104 12-13 0.23258232774848864 1104 14-15 0.2232974694614711 1104 16-17 0.33729328115131096 1104 18-19 0.24998406110287164 1104 20-21 0.23050002140909243 1104 22-23 0.2944332252898292 1104 24-25 0.34991954405723646 1104 26-27 0.42443421317756247 1104 28-29 0.22456079125402795 1104 30-31 0.23907339047539722 1104 32-33 0.27161575944562344 1104 34-35 0.3699060816618953 1104 36-37 0.3787141876488178 1104 38-39 0.27269814042330864 1104 40-41 0.3172776834735629 1104 42-43 0.4331988793280033 1104 44-45 0.40877415210761825 1104 46-47 0.42311513906650333 1104 48-49 0.4931816096189081 1104 50-51 0.5667782193711588 1104 52-53 0.5102989505565887 1104 54-55 0.4726735140423344 1104 56-57 0.5157637923399818 1104 58-59 0.6479313594009284 1104 60-61 0.5639002384978866 1104 62-63 0.5577964932109367 1104 64-65 0.4615637703055029 1104 66-67 0.5215570859037655 1104 68-69 0.4274146064765816 1104 70-71 0.3609186302184 1104 72-73 0.44020357948918587 1104 74-75 0.4172689785279218 1104 76-77 0.4118357485568751 1104 78-79 0.4139386710164672 1104 80-81 0.4032686058036603 1104 82-83 0.4329643167377526 1104 84-85 0.47208797628546506 1104 86-87 0.47287351080429474 1104 88-89 0.41101281074752194 1104 90-91 0.37198872606655087 1104 92-93 0.44859305013224926 1104 94-95 0.4881867524392405 1104 96-97 0.48773292892995457 1104 98-99 0.45577641058487117 1104 100-101 0.4364097774760296 1104 102-103 0.3226396519074122 1104 104-105 0.48970472575026136 1104 106-107 0.4206043746844088 1104 108-109 0.4416863930239501 1104 110-111 0.47931460049658625 1104 112-113 0.5222230893591586 1104 114-115 0.6189796643498546 1104 116-117 0.5994898118146175 1104 118-119 0.5469583812836056 1104 120-121 0.6336504011814235 1104 122-123 0.6148028953411 1104 124-125 0.638817944243435 1105 1 0.11927174161947107 1105 2 0.2594118968708088 1105 3 0.1823447700607872 1105 4 0.14041536163905732 1105 5 0.1481690027660818 1105 6 0.18605808095816911 1105 7 0.21266954084547507 1105 8 0.11754776448932347 1105 9 0.23481612098818516 1105 10-11 0.2087216185055496 1105 12-13 0.15587049261529273 1105 14-15 0.2428884613382536 1105 16-17 0.2407858224869983 1105 18-19 0.24656447960311567 1105 20-21 0.2325647035708016 1105 22-23 0.27404410229837595 1105 24-25 0.3006628586337925 1105 26-27 0.2101190448586081 1105 28-29 0.18056150577324814 1105 30-31 0.2563185129054517 1105 32-33 0.2782640166740933 1105 34-35 0.29516884049870384 1105 36-37 0.3360360667647697 1105 38-39 0.42312792489407514 1105 40-41 0.37442075879444303 1105 42-43 0.390218982333991 1105 44-45 0.4904621998984382 1105 46-47 0.5923010530624424 1105 48-49 0.5843336539345714 1105 50-51 0.5863847736930836 1105 52-53 0.5286067656685489 1105 54-55 0.5441207963077801 1105 56-57 0.49582066389412915 1105 58-59 0.5120126367354985 1105 60-61 0.5348106454045976 1105 62-63 0.659165911053698 1105 64-65 0.5695436508671179 1105 66-67 0.4848248823330721 1105 68-69 0.44577200875843914 1105 70-71 0.47080621523429755 1105 72-73 0.48520127798720836 1105 74-75 0.413724994128259 1105 76-77 0.31774414201182566 1105 78-79 0.2404234974391386 1105 80-81 0.3129781989781435 1105 82-83 0.3976692711436556 1105 84-85 0.34353840970439364 1105 86-87 0.3168513955064931 1105 88-89 0.3355625969427223 1105 90-91 0.41973237451072265 1105 92-93 0.45747785304693167 1105 94-95 0.45389176310193236 1105 96-97 0.44128164005984516 1105 98-99 0.40580520594142655 1105 100-101 0.4136163050421473 1105 102-103 0.4317943520848253 1105 104-105 0.45372589918182626 1105 106-107 0.3730022381636786 1105 108-109 0.36156522247289047 1105 110-111 0.2679358045890652 1105 112-113 0.3310781786363606 1105 114-115 0.3356134436712921 1105 116-117 0.32545206872874033 1105 118-119 0.32829868092157355 1105 120-121 0.2920426478745952 1105 122-123 0.2195088627558519 1105 124-125 0.1976237846647706 1106 1 0.073464437712218 1106 2 0.1385577127838431 1106 3 0.047192304336512336 1106 4 0.01239501638323759 1106 5 0.04011986145264501 1106 6 0.044447579797918024 1106 7 0.05083510333341934 1106 8 0.06129709035481312 1106 9 0.08364513078973346 1106 10-11 0.17136244458626493 1106 12-13 0.1705214517417204 1106 14-15 0.20593738000176387 1106 16-17 0.16710799198791193 1106 18-19 0.1543588104411242 1106 20-21 0.09326610032803018 1106 22-23 0.005139985253784118 1106 24-25 0.11191974222746381 1106 26-27 0.10267729255417635 1106 28-29 0.1735228982753867 1106 30-31 0.07012864694070231 1106 32-33 0.1162895570949587 1106 34-35 0.087490386579816 1106 36-37 0.0821673510590415 1106 38-39 0.2414024374144219 1106 40-41 0.19275345716316394 1106 42-43 0.24133990103707958 1106 44-45 0.1863409991721099 1106 46-47 0.13262325225001348 1106 48-49 0.10888461850354503 1106 50-51 0.08997517698632151 1106 52-53 0.02451030382332675 1106 54-55 -0.04497826311995823 1106 56-57 0.005369575135972582 1106 58-59 -0.003689625882572045 1106 60-61 0.03717320855059114 1106 62-63 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-1.5461532399446263 2216 94-95 -1.6400997130318586 2216 96-97 -1.6703230301800183 2216 98-99 -1.666744984334457 2216 100-101 -1.6622642374754193 2216 102-103 -1.7103181111398094 2216 104-105 -1.7091492712029854 2216 106-107 -1.848827740927561 2216 108-109 -1.7981835023394552 2216 110-111 -1.8433543090830469 2216 112-113 -1.8838882995019546 2216 114-115 -1.8351736262034422 2216 116-117 -1.798122897502683 2216 118-119 -1.9269237599140396 2216 120-121 -2.2064320611605766 2216 122-123 -2.277758319125688 2216 124-125 -2.6062946033253382 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11.0 3 11.0 4 46.0 5 126.0 6 350.0 7 835.0 8 1586.0 9 2797.0 10 4769.0 11 6866.0 12 8911.0 13 13140.0 14 20266.0 15 18438.0 16 7427.0 17 5472.0 18 5208.0 19 4605.0 20 4330.0 21 4388.0 22 4473.0 23 5128.0 24 6411.0 25 8392.0 26 10520.0 27 13767.0 28 17866.0 29 22660.0 30 28507.0 31 36450.0 32 46262.0 33 59251.0 34 81097.0 35 120776.0 36 223284.0 37 450848.0 38 217782.0 39 1116.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.908753194065795 19.167541792234356 13.609200277759726 22.314504735940126 2 16.544627630111417 21.071762505045594 38.781708692216434 23.601901172626558 3 19.11981652428813 26.244116128433 30.673786959455583 23.96228038782329 4 12.645372265007115 17.222771641583094 36.73195498889475 33.399901104515045 5 12.23838456137667 39.120540482948726 34.27322746234732 14.367847493327288 6 30.763530514174565 37.409539316419114 16.975328035230834 14.851602134175494 7 27.193458145627698 34.54116046475414 20.87084031111099 17.39454107850717 8 27.021005728835135 34.40087639976724 19.378597596457247 19.199520274940376 9 27.379160371868878 14.851397240214947 20.194758539297293 37.574683848618875 10-11 24.88526741000249 26.99804086354992 27.04110293542636 21.075588791021232 12-13 26.078834553714707 24.864088070998296 28.131438506404827 20.92563886888217 14-15 22.9411913354442 25.4712219602615 27.190384736219514 24.39720196807479 16-17 21.303117269489935 29.18361032833709 27.601071190015343 21.912201212157637 18-19 20.951500233579115 26.162465885155566 31.905438705288724 20.980595175976593 20-21 21.84080832033395 25.143152580434542 32.46981911961163 20.54621997961988 22-23 21.586364170329716 25.336060244288237 32.213257732015094 20.864317853366956 24-25 21.341857377411944 25.655865567706527 32.362557131266 20.639719923615534 26-27 21.0634747830173 25.884288184721466 32.26530079799368 20.786936234267557 28-29 20.81469936592149 25.544642296123683 32.68782629363216 20.952832044322662 30-31 20.923156569036973 25.4643238635898 32.70776930579194 20.904750261581288 32-33 20.85885401441907 25.654977693877495 32.38625653270244 21.099911759000992 34-35 21.349916539860068 25.58282087077201 32.134817494119545 20.932445095248372 36-37 21.5812759703095 25.324586182497683 31.80459672770685 21.28954111948596 38-39 21.09550653884926 25.619633485683373 32.039541802465834 21.245318173001532 40-41 21.329563739779207 25.411461221769027 32.16548329021454 21.093491748237227 42-43 21.391441715863984 25.479656761637294 31.55831418713102 21.5705873353677 44-45 21.41042855620788 25.731027502233346 31.241001740232704 21.617542201326074 46-47 21.75570902872067 25.996194436172797 30.409337154378036 21.838759380728494 48-49 21.959202880535894 26.227212376687987 29.735919004051436 22.077665738724686 50-51 21.98860516387419 26.4311160164243 29.269546200856183 22.31073261884533 52-53 22.14770532423786 26.591957775452613 29.131003734533923 22.129333165775606 54-55 21.775925232827838 26.555725693429462 29.222010802009603 22.4463382717331 56-57 21.843915878735558 26.881575388683842 29.028625052247957 22.24588368033264 58-59 21.893022131279658 26.239232822373324 29.270741415626034 22.59700363072098 60-61 21.93830369656024 26.49756995762793 29.442954789464626 22.121171556347203 62-63 21.845657477400195 26.313916670992203 29.61568039820458 22.224745453403017 64-65 21.679078687476608 26.196683176566687 29.592015145761565 22.53222299019514 66-67 21.73644899642938 26.66008501733403 29.14309247820611 22.460373508030475 68-69 21.723472378928157 26.300120477648804 29.407815475231054 22.56859166819199 70-71 21.66774122165975 26.40342118275722 29.374554355635812 22.55428323994722 72-73 21.943152853626486 26.326073712651244 29.06724756381081 22.663525869911457 74-75 21.597394295205756 26.69191187920545 28.89687823561713 22.81381558997167 76-77 21.653535240395254 26.682213565072953 28.903161650407196 22.761089544124598 78-79 21.681059329095216 26.52779181680841 28.870378616719893 22.920770237376484 80-81 21.379626164139186 26.916065872042356 28.69481864152572 23.009489322292737 82-83 21.60985867780561 26.825502741481195 28.470596350701964 23.094042230011226 84-85 21.61655188051677 26.608383441289686 28.282913482842183 23.492151195351365 86-87 21.538350685575193 26.361861857759884 28.562149802072433 23.537637654592494 88-89 21.538350685575193 26.408919170698525 28.48910510513792 23.563625038588363 90-91 21.44867542884306 26.57993732976727 28.564369486645013 23.40701775474466 92-93 21.53324304333305 26.200935546462812 28.53683073903253 23.728990671171605 94-95 21.33561723361213 26.002484697731536 28.584132083744834 24.077765984911505 96-97 21.522315537548188 25.894923269849162 28.507907972996232 24.074853219606418 98-99 21.2122453927811 25.96874962648786 28.359671275166182 24.459333705564863 100-101 21.07003012716793 25.966873092293223 28.633923150109247 24.3291736304296 102-103 21.376104210642428 25.697428098884544 28.447662626787647 24.478805063685382 104-105 21.123728201707696 25.558300282202524 28.59647702779915 24.721494488290627 106-107 21.08105560341029 25.71849847088743 28.52698335497612 24.67346257072615 108-109 21.184383112447055 25.794268342669763 27.99986336931275 25.021485175570433 110-111 21.000143448501987 25.93135647635832 28.153954397038127 24.91454567810156 112-113 20.957688671253763 25.814241680648387 28.214645829803747 25.0134238182941 114-115 20.61444678542833 25.87192540914842 28.102703153223317 25.410924652199935 116-117 20.503346592180296 25.855503047713064 28.219824737285254 25.421325622821385 118-119 20.54163181509707 25.901800373169376 27.87813625702852 25.67843155470504 120-121 20.427888439955336 26.076526832347398 27.99521395617484 25.500370771522423 122-123 20.497757801586786 26.030369578507685 27.80175755567927 25.670115064226263 124-125 20.351654101492723 25.929921917638854 27.816815542973305 25.901608437895117 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1256.0 1 839.5 2 597.0 3 765.5 4 527.5 5 400.5 6 944.5 7 2226.0 8 3557.5 9 4069.0 10 3225.5 11 2086.0 12 1838.0 13 1923.0 14 2078.0 15 2379.0 16 2676.5 17 2916.5 18 3157.5 19 3333.5 20 3598.0 21 4011.0 22 4431.0 23 5011.5 24 6015.5 25 7386.0 26 9319.5 27 11343.0 28 13140.0 29 15247.0 30 17922.5 31 20701.5 32 24381.0 33 28302.0 34 32295.5 35 35347.0 36 38822.5 37 42586.5 38 43610.5 39 43717.0 40 43195.5 41 44120.5 42 45845.5 43 47754.0 44 49404.5 45 50667.0 46 50309.5 47 49056.0 48 50658.0 49 51287.0 50 50212.5 51 48718.5 52 46980.5 53 46800.5 54 46490.5 55 43589.5 56 39121.5 57 34763.0 58 31641.5 59 28708.0 60 25340.5 61 21483.0 62 17838.0 63 13920.0 64 10459.0 65 8281.0 66 5857.0 67 4398.0 68 3020.0 69 1598.0 70 1163.5 71 1023.0 72 838.5 73 715.0 74 581.0 75 467.5 76 291.0 77 114.5 78 58.0 79 22.0 80 9.0 81 5.5 82 2.5 83 1.0 84 0.5 85 1.0 86 1.0 87 0.5 88 0.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1691741134238327 2 0.0015708536975164121 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 4.439369145155078E-4 12-13 0.005497987941307442 14-15 0.0 16-17 4.097879210912379E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 6.829798684853965E-5 94-95 6.829798684853966E-4 96-97 0.0054638389478831725 98-99 0.0020147906120319197 100-101 0.003073409408184284 102-103 0.004132028204336649 104-105 0.010415442994402297 106-107 0.01714279469898345 108-109 0.02540685110765675 110-111 0.0158451329488612 112-113 0.024314083318080115 114-115 0.02731919473941586 116-117 0.0252361061405354 118-119 0.015401196034345692 120-121 0.022709080627139434 122-123 0.014137683277647708 124-125 0.01400108730395063 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1464172.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.61771929612111 #Duplication Level Percentage of deduplicated Percentage of total 1 79.5709745783485 41.07272229903982 2 11.110333499822781 11.469801517602862 3 3.7206988950099786 5.761619734440392 4 1.6886780294868837 3.4866283403032354 5 0.940320480763762 2.426859931222876 6 0.5684829790164281 1.7606276901295619 7 0.38339959326934503 1.3853148808536841 8 0.2827168424136412 1.167455888959443 9 0.19960347961170405 0.9272768743013378 >10 1.3202365720062175 13.34549280618435 >50 0.13519914184862575 4.771699663212945 >100 0.07427926779731482 6.653174722271333 >500 0.0032062993293641537 1.142376580107882 >1k 0.0010687664431213847 0.9659791823102264 >5k 2.671916107803462E-4 0.8124762083080446 >10k+ 5.343832215606924E-4 2.8504936807521077 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10629 0.725939302213128 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10039 0.6856434899724896 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5891 0.40234344052474713 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2925 0.1997716115319785 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2019 0.13789363544720157 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 6.829798684853965E-5 0.0 14-15 0.0 0.0 0.0 1.365959736970793E-4 0.0 16-17 0.0 0.0 0.0 1.365959736970793E-4 0.0 18-19 0.0 0.0 0.0 2.731919473941586E-4 0.0 20-21 0.0 0.0 0.0 3.0734094081842845E-4 0.0 22-23 0.0 0.0 0.0 6.146818816368569E-4 0.0 24-25 0.0 0.0 0.0 0.0011269167830009043 0.0 26-27 0.0 0.0 0.0 0.0025270255133959672 0.0 28-29 0.0 0.0 0.0 0.0051564980070647436 0.0 30-31 0.0 0.0 0.0 0.014171832271071978 0.0 32-33 0.0 0.0 0.0 0.033226970601814544 0.0 34-35 0.0 0.0 0.0 0.06307319085462637 0.0 36-37 0.0 0.0 0.0 0.11040369574066436 0.0 38-39 0.0 0.0 0.0 0.17876998057605253 0.0 40-41 0.0 0.0 0.0 0.2528049983198695 0.0 42-43 0.0 0.0 0.0 0.32622533418204963 0.0 44-45 0.0 0.0 0.0 0.42098879093439845 0.0 46-47 0.0 0.0 0.0 0.5325877014449122 0.0 48-49 0.0 0.0 0.0 0.660509830812227 0.0 50-51 0.0 0.0 0.0 0.8041063481612816 0.0 52-53 0.0 0.0 0.0 0.9411804077663006 0.0 54-55 0.0 0.0 0.0 1.089045549293389 0.0 56-57 0.0 0.0 0.0 1.2682253177905327 0.0 58-59 0.0 0.0 0.0 1.4688165051646938 0.0 60-61 0.0 0.0 0.0 1.6958048644558152 0.0 62-63 0.0 0.0 0.0 1.9199930062861466 0.0 64-65 0.0 0.0 0.0 2.151693926669817 0.0 66-67 0.0 0.0 0.0 2.4193878861226685 0.0 68-69 0.0 0.0 0.0 2.728470425605735 0.0 70-71 0.0 0.0 0.0 3.0837907021852624 0.0 72-73 0.0 0.0 0.0 3.4441650297915816 0.0 74-75 0.0 0.0 0.0 3.802490417792445 0.0 76-77 0.0 0.0 0.0 4.1868031897891775 0.0 78-79 0.0 0.0 0.0 4.633779364719446 0.0 80-81 0.0 0.0 0.0 5.13607690899703 0.0 82-83 0.0 0.0 0.0 5.648824045262442 0.0 84-85 0.0 0.0 0.0 6.152863188204664 0.0 86-87 0.0 0.0 0.0 6.677255131227752 0.0 88-89 0.0 0.0 0.0 7.258368552328552 0.0 90-91 0.0 0.0 0.0 7.898354838092793 0.0 92-93 0.0 0.0 0.0 8.580412683755734 0.0 94-95 0.0 0.0 0.0 9.254821154891639 0.0 96-97 0.0 0.0 0.0 9.91857514007917 0.0 98-99 0.0 0.0 0.0 10.626415475777435 0.0 100-101 0.0 0.0 0.0 11.417681802411192 0.0 102-103 0.0 0.0 0.0 12.257576295681108 0.0 104-105 0.0 0.0 0.0 13.099007493655117 0.0 106-107 0.0 0.0 0.0 13.904104162625703 0.0 108-109 0.0 0.0 0.0 14.73040735651276 0.0 110-111 0.0 0.0 0.0 15.631735888952939 0.0 112-113 0.0 0.0 0.0 16.581112055141062 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4880 0.0 72.94584 1 GTATCAA 9230 0.0 56.074326 1 TGGTATC 1965 0.0 46.63036 2 GTGGTAT 2105 0.0 43.906178 1 ATCAACG 11915 0.0 43.19197 3 TCAACGC 12055 0.0 42.69182 4 TATCAAC 12095 0.0 42.552082 2 CAACGCA 12205 0.0 42.167137 5 AACGCAG 12705 0.0 41.024193 6 ACGCAGA 14605 0.0 35.60821 7 GTACATG 9535 0.0 35.207382 1 CGCAGAG 14750 0.0 35.056458 8 TACATGG 9905 0.0 33.218594 2 ACATGGG 9660 0.0 33.073345 3 GCAGAGT 15780 0.0 32.579697 9 ATGGGAG 2505 0.0 29.68917 5 CATGGGA 7030 0.0 28.690634 4 GAGTACT 11055 0.0 27.718296 12-13 CAGAGTA 15635 0.0 27.534266 10-11 CATGGGG 2970 0.0 27.244463 4 >>END_MODULE