FastQCFastQC Report
Thu 26 May 2016
SRR1364101_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364101_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2156787
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN88810.4117699151562022No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21500.051.2080841
GTATCAA34400.042.731071
ATCAACG35350.040.0712853
CAACGCA36950.038.1803935
TCAACGC37600.037.6786774
AACGCAG39850.035.4018976
TATCAAC42000.034.5736962
GTACATG70450.033.7896921
TACATGG74250.030.7779732
ACATGGG76300.028.4717433
ACGCAGA48850.028.1497277
CGCAGAG49400.027.1132978
CATGGGG45050.025.2374274
GCAGAGT61300.020.8787619
GTGGTAT14000.020.4041481
AGTACAT52500.020.4041462
GAGTACA55300.020.016731
TACCGTA1500.004694068319.8429167
GGACCGT3051.639255E-719.5167126
CAGAGTA57350.019.41064610-11
Produced by FastQC (version 0.11.5)