##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364101_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2156787 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.96753921458169 31.0 31.0 34.0 28.0 34.0 2 31.02564740978131 33.0 31.0 34.0 27.0 34.0 3 31.11865427601335 33.0 31.0 34.0 27.0 34.0 4 34.595259522613965 37.0 35.0 37.0 32.0 37.0 5 34.640953418209584 37.0 35.0 37.0 32.0 37.0 6 34.68212299128287 37.0 35.0 37.0 32.0 37.0 7 34.58423664460144 37.0 35.0 37.0 31.0 37.0 8 34.55741758458299 37.0 35.0 37.0 31.0 37.0 9 36.08452295011051 39.0 37.0 39.0 31.0 39.0 10-11 35.99706044222262 39.0 37.0 39.0 30.5 39.0 12-13 35.89863069463976 39.0 37.0 39.0 30.0 39.0 14-15 37.14836560123925 40.0 37.0 41.0 31.0 41.0 16-17 37.150548709724234 40.0 37.0 41.0 31.0 41.0 18-19 37.144571763461116 40.0 37.0 41.0 31.0 41.0 20-21 37.161923963747924 40.0 37.0 41.0 31.0 41.0 22-23 37.10976675026324 40.0 37.0 41.0 31.0 41.0 24-25 37.1139185278843 40.0 37.0 41.0 31.0 41.0 26-27 37.06931908436021 40.0 37.0 41.0 30.5 41.0 28-29 37.016704709366294 40.0 37.0 41.0 30.0 41.0 30-31 36.9658770198448 40.0 37.0 41.0 30.0 41.0 32-33 36.862667708957815 40.0 37.0 41.0 30.0 41.0 34-35 36.8422876250645 40.0 37.0 41.0 30.0 41.0 36-37 36.78258539206699 40.0 37.0 41.0 30.0 41.0 38-39 36.70577715833784 40.0 37.0 41.0 30.0 41.0 40-41 36.63891195560804 40.0 36.5 41.0 30.0 41.0 42-43 36.533799814260746 40.0 36.0 41.0 30.0 41.0 44-45 36.450799963093246 40.0 36.0 41.0 30.0 41.0 46-47 36.34584337720878 40.0 36.0 41.0 29.5 41.0 48-49 36.246643502580454 40.0 36.0 41.0 29.0 41.0 50-51 36.12762572289243 40.0 35.0 41.0 29.0 41.0 52-53 35.98864027833996 39.5 35.0 41.0 28.0 41.0 54-55 35.816248428797095 39.0 35.0 41.0 28.0 41.0 56-57 35.636471751730696 39.0 35.0 41.0 28.0 41.0 58-59 35.440431762617266 39.0 35.0 41.0 28.0 41.0 60-61 35.20378437926415 38.5 34.5 41.0 27.0 41.0 62-63 34.94358715070148 38.0 34.0 40.0 27.0 41.0 64-65 34.666726477858035 37.5 34.0 40.0 26.0 41.0 66-67 34.3648698272013 37.0 34.0 40.0 26.0 41.0 68-69 34.050704821570235 36.5 34.0 39.5 26.0 41.0 70-71 33.709884657131184 36.0 33.5 39.0 26.0 41.0 72-73 33.35439591392196 35.5 33.0 39.0 26.0 40.5 74-75 33.07155922212068 35.0 33.0 38.0 25.5 40.0 76-77 32.8452533792164 35.0 33.0 37.0 26.0 39.0 78-79 32.5374765797457 35.0 33.0 37.0 25.0 39.0 80-81 32.313736358759584 35.0 33.0 36.0 25.5 39.0 82-83 32.20668916309306 35.0 33.0 36.0 26.0 37.0 84-85 31.983210905852083 35.0 33.0 36.0 25.0 37.0 86-87 31.77986838755983 35.0 33.0 35.0 25.0 37.0 88-89 31.597325095153113 35.0 33.0 35.0 25.0 36.0 90-91 31.447515215920717 35.0 33.0 35.0 24.0 36.0 92-93 31.321113072361804 35.0 33.0 35.0 24.0 36.0 94-95 31.214370264657568 35.0 33.0 35.0 23.5 36.0 96-97 31.123413206774707 35.0 33.0 35.0 23.0 35.0 98-99 31.036952652255415 35.0 33.0 35.0 23.0 35.0 100-101 30.95471435055942 35.0 33.0 35.0 21.0 35.0 102-103 29.860061981085753 34.0 30.0 35.0 18.5 35.0 104-105 30.669116607249578 35.0 32.0 35.0 19.5 35.0 106-107 30.757095160532774 35.0 32.0 35.0 19.0 35.0 108-109 30.72488451571713 35.0 32.0 35.0 19.0 35.0 110-111 30.644923675819634 35.0 32.0 35.0 18.0 35.0 112-113 30.554731644803127 35.0 32.0 35.0 16.0 35.0 114-115 30.440094455317098 35.0 32.0 35.0 10.5 35.0 116-117 30.330612155952352 35.0 32.0 35.0 4.5 35.0 118-119 30.18949970488509 35.0 32.0 35.0 2.0 35.0 120-121 30.028448567243778 35.0 32.0 35.0 2.0 35.0 122-123 29.845516270266835 35.0 32.0 35.0 2.0 35.0 124-125 28.99091889927007 34.5 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4288497206039885 1101 2 0.46780671902108395 1101 3 0.4398170652127469 1101 4 0.4789940903013914 1101 5 0.49886416533846045 1101 6 0.4917406314938475 1101 7 0.44248321386176315 1101 8 0.4735345636972781 1101 9 0.4586092773142738 1101 10-11 0.5234875141370594 1101 12-13 0.6077938236863645 1101 14-15 0.5566127512504835 1101 16-17 0.5326985905086872 1101 18-19 0.5115888612914645 1101 20-21 0.54710350415737 1101 22-23 0.700075274530775 1101 24-25 0.5934210804241715 1101 26-27 0.5351198091356864 1101 28-29 0.588617492988945 1101 30-31 0.5857615595205559 1101 32-33 0.5843152015296269 1101 34-35 0.6813895877024976 1101 36-37 0.7493285685236231 1101 38-39 0.6110185375327433 1101 40-41 0.7530755979393859 1101 42-43 0.7357595350402804 1101 44-45 0.7686962856368638 1101 46-47 0.7410637394776458 1101 48-49 0.7175093771578531 1101 50-51 0.65576226589166 1101 52-53 0.680073563441276 1101 54-55 0.6619598624605629 1101 56-57 0.5850638845509337 1101 58-59 0.5933330502870433 1101 60-61 0.6415421165267503 1101 62-63 0.6925621300552791 1101 64-65 0.6548326706638292 1101 66-67 0.6828590751638615 1101 68-69 0.6636373617599958 1101 70-71 0.7666748528574061 1101 72-73 0.680241071018628 1101 74-75 0.6268161598029351 1101 76-77 0.6827955852983081 1101 78-79 0.5617011151002202 1101 80-81 0.5441003847465353 1101 82-83 0.5582943297737089 1101 84-85 0.5616310208365007 1101 86-87 0.49724340168767256 1101 88-89 0.5790558246710305 1101 90-91 0.5750201110948971 1101 92-93 0.6566125350885379 1101 94-95 0.6123297921144797 1101 96-97 0.5371083920519943 1101 98-99 0.49357558128054535 1101 100-101 0.5243241885055916 1101 102-103 0.583037645764076 1101 104-105 0.5241289270956493 1101 106-107 0.5508814397671173 1101 108-109 0.5898761880488372 1101 110-111 0.5748226279106241 1101 112-113 0.5930877306342843 1101 114-115 0.687273070302421 1101 116-117 0.6637902718118731 1101 118-119 0.6601818434458053 1101 120-121 0.7884840348635507 1101 122-123 0.7057373492284711 1101 124-125 0.8261441439689285 1102 1 0.3551730204032424 1102 2 0.3929364991526647 1102 3 0.3309184281186006 1102 4 0.448366837141009 1102 5 0.42425903437634105 1102 6 0.45721444574287773 1102 7 0.4168350757753174 1102 8 0.39316877722872334 1102 9 0.45290009538187803 1102 10-11 0.36193760669416974 1102 12-13 0.40688969989052026 1102 14-15 0.45679653097408845 1102 16-17 0.5030589150591069 1102 18-19 0.47725613038959835 1102 20-21 0.5066213969436362 1102 22-23 0.4529631803379459 1102 24-25 0.517582394792349 1102 26-27 0.4880140061574636 1102 28-29 0.5598036347776727 1102 30-31 0.5700421019376378 1102 32-33 0.5037622037658664 1102 34-35 0.5984003170830405 1102 36-37 0.5803458769895045 1102 38-39 0.5835591015687314 1102 40-41 0.5968557790882372 1102 42-43 0.5222148836212313 1102 44-45 0.6132354160384921 1102 46-47 0.6270757004111118 1102 48-49 0.6196566830881878 1102 50-51 0.7057858102857111 1102 52-53 0.665097184092474 1102 54-55 0.6221188395471984 1102 56-57 0.7168859442183333 1102 58-59 0.6593081599543495 1102 60-61 0.580877268593035 1102 62-63 0.4721511421630069 1102 64-65 0.4717896387559435 1102 66-67 0.5241721469733136 1102 68-69 0.5215308819995954 1102 70-71 0.5456209695575325 1102 72-73 0.5559792878596426 1102 74-75 0.5082249533958034 1102 76-77 0.6263222590950193 1102 78-79 0.5162908421186927 1102 80-81 0.555586808114775 1102 82-83 0.5571182539283441 1102 84-85 0.5388846101650699 1102 86-87 0.5624536045126156 1102 88-89 0.546696867029965 1102 90-91 0.5513510842695375 1102 92-93 0.5531092341072998 1102 94-95 0.5416779746207112 1102 96-97 0.5851325693803631 1102 98-99 0.5761039251087681 1102 100-101 0.5927447218862021 1102 102-103 0.5944708797724765 1102 104-105 0.5655213063376898 1102 106-107 0.5299948932435825 1102 108-109 0.5263638029349984 1102 110-111 0.5110984385717536 1102 112-113 0.5864087484384228 1102 114-115 0.591417550905458 1102 116-117 0.573027252333894 1102 118-119 0.5959063149361228 1102 120-121 0.6210445496755455 1102 122-123 0.5419143592238775 1102 124-125 0.5353030562369838 1103 1 0.31366052395675226 1103 2 0.38393011363780616 1103 3 0.3896934636352434 1103 4 0.3670311822724628 1103 5 0.4052046388187378 1103 6 0.372718636161963 1103 7 0.29628841777562087 1103 8 0.3570219678341928 1103 9 0.39819282623175667 1103 10-11 0.4060844120249101 1103 12-13 0.39787407847808254 1103 14-15 0.5817602108068911 1103 16-17 0.5238978707450883 1103 18-19 0.5094959703942905 1103 20-21 0.521430881619338 1103 22-23 0.5303470255339562 1103 24-25 0.5832943744349635 1103 26-27 0.5537316196975794 1103 28-29 0.6398126914988325 1103 30-31 0.5826427755301182 1103 32-33 0.32495827466726723 1103 34-35 0.46994666863565726 1103 36-37 0.5282593861336835 1103 38-39 0.6574174199445295 1103 40-41 0.7490617085714177 1103 42-43 0.7412264615577087 1103 44-45 0.6459622187425396 1103 46-47 0.7081230567370298 1103 48-49 0.6672179073913753 1103 50-51 0.6651496619801094 1103 52-53 0.6730463825920481 1103 54-55 0.7394120602690748 1103 56-57 0.7138305707638892 1103 58-59 0.7125073917493339 1103 60-61 0.8486109769776959 1103 62-63 0.801497661772018 1103 64-65 0.713609819894053 1103 66-67 0.7183971157051801 1103 68-69 0.7272372720769908 1103 70-71 0.6783776226825182 1103 72-73 0.7732532702068866 1103 74-75 0.7480882787363967 1103 76-77 0.760368797674559 1103 78-79 0.7470040251987484 1103 80-81 0.691957190881773 1103 82-83 0.6015267122372308 1103 84-85 0.6055179167697133 1103 86-87 0.6162375148032346 1103 88-89 0.5872819408220167 1103 90-91 0.5136461789550388 1103 92-93 0.5804288343998181 1103 94-95 0.6106330011563657 1103 96-97 0.5477547995577723 1103 98-99 0.5790446348948848 1103 100-101 0.6001192628390051 1103 102-103 0.562092639842696 1103 104-105 0.6506337044403416 1103 106-107 0.6330644160931023 1103 108-109 0.6082376335549178 1103 110-111 0.6372690193194508 1103 112-113 0.6253109493343416 1103 114-115 0.7327216577950857 1103 116-117 0.7623119394558593 1103 118-119 0.7953881718465752 1103 120-121 0.7055948048726322 1103 122-123 0.8110645772187652 1103 124-125 0.7829471173054792 1104 1 0.38956513660173187 1104 2 0.4505010517387227 1104 3 0.38468290673138483 1104 4 0.3801273741488629 1104 5 0.39367706849002104 1104 6 0.2925784386946191 1104 7 0.3413335306288374 1104 8 0.4008706129990074 1104 9 0.40841047072920134 1104 10-11 0.49597768889121596 1104 12-13 0.4583888048990019 1104 14-15 0.4516141034805088 1104 16-17 0.5772901019566135 1104 18-19 0.570034113237817 1104 20-21 0.44140414229485714 1104 22-23 0.40118942576187067 1104 24-25 0.4825526034955203 1104 26-27 0.5687991119604092 1104 28-29 0.5577817572895043 1104 30-31 0.4616268428846695 1104 32-33 -0.17957363940784177 1104 34-35 0.5073091041220223 1104 36-37 0.5741575258104206 1104 38-39 0.63749520149495 1104 40-41 0.6305065910516419 1104 42-43 0.6193556901036672 1104 44-45 0.6581143663908833 1104 46-47 0.6700756908894334 1104 48-49 0.65678993324088 1104 50-51 0.7099682766979925 1104 52-53 0.6569039650248243 1104 54-55 0.5874620576765466 1104 56-57 0.4662346568181306 1104 58-59 0.5141329375053019 1104 60-61 0.5626156145444341 1104 62-63 0.6001547578122484 1104 64-65 0.7092468385820325 1104 66-67 0.7312999827116116 1104 68-69 0.680700059199971 1104 70-71 0.6452056117749265 1104 72-73 0.7055202921341319 1104 74-75 0.7233932915791925 1104 76-77 0.5994635466532969 1104 78-79 0.6629552365247164 1104 80-81 0.5934723170544771 1104 82-83 0.5498253418747296 1104 84-85 0.6396203123254978 1104 86-87 0.6280470578874962 1104 88-89 0.5709959582828645 1104 90-91 0.5695874550274063 1104 92-93 0.5913339181609203 1104 94-95 0.5548403078607649 1104 96-97 0.5303906858944565 1104 98-99 0.47608290346229865 1104 100-101 0.4137009830874625 1104 102-103 0.5184378770817197 1104 104-105 0.5100484722860266 1104 106-107 0.5164861742984677 1104 108-109 0.4124696501605065 1104 110-111 0.5259314565341704 1104 112-113 0.4931331885799324 1104 114-115 0.5796439035638237 1104 116-117 0.5591537052102318 1104 118-119 0.5861829252298314 1104 120-121 0.5146164555854682 1104 122-123 0.6030793540964972 1104 124-125 0.5947429171798113 1105 1 0.4198815895610899 1105 2 0.40734253052957925 1105 3 0.4200256918906753 1105 4 0.34518201858450226 1105 5 0.37921711755849685 1105 6 0.37360372170317646 1105 7 0.32006464632210196 1105 8 0.3629776783128804 1105 9 0.39541933082032443 1105 10-11 0.49570543397184963 1105 12-13 0.45490496608756814 1105 14-15 0.6178361155610048 1105 16-17 0.4892612958499143 1105 18-19 0.5095272354583713 1105 20-21 0.5044940279534771 1105 22-23 0.43063591472602525 1105 24-25 0.5530363296497427 1105 26-27 0.5215297406103829 1105 28-29 0.5003389564264751 1105 30-31 0.5102399928257313 1105 32-33 -0.0069519297948801295 1105 34-35 0.46178478154460834 1105 36-37 0.5282593861336835 1105 38-39 0.5698399962767624 1105 40-41 0.62489222236443 1105 42-43 0.5748664767468412 1105 44-45 0.5077879734243353 1105 46-47 0.4571401968136044 1105 48-49 0.4598941513998511 1105 50-51 0.5793677056738815 1105 52-53 0.5587796790894473 1105 54-55 0.5180596700265099 1105 56-57 0.5493174985563769 1105 58-59 0.6031055322170147 1105 60-61 0.506218733442175 1105 62-63 0.5807344758733564 1105 64-65 0.5935113292576588 1105 66-67 0.5882002935999822 1105 68-69 0.6281696531333267 1105 70-71 0.5058146491589213 1105 72-73 0.4793721843200487 1105 74-75 0.5194045151957809 1105 76-77 0.5337260743624199 1105 78-79 0.462609896664091 1105 80-81 0.47103000551064156 1105 82-83 0.5125548667116604 1105 84-85 0.40249509668367267 1105 86-87 0.42482483414800853 1105 88-89 0.3692041396996615 1105 90-91 0.34526675523269645 1105 92-93 0.41459268152616247 1105 94-95 0.40112637523660766 1105 96-97 0.34094123256640074 1105 98-99 0.3187979364856517 1105 100-101 0.32964604619825977 1105 102-103 0.4134087853491657 1105 104-105 0.4665424491082284 1105 106-107 0.5269632946436786 1105 108-109 0.4763002000642338 1105 110-111 0.3631181784597679 1105 112-113 0.35881862803407927 1105 114-115 0.40724807734268964 1105 116-117 0.4966313729101586 1105 118-119 0.5380349130036777 1105 120-121 0.544122118032611 1105 122-123 0.6087517583151119 1105 124-125 0.6281975561532889 1106 1 0.10990247757559146 1106 2 0.18929031394082685 1106 3 0.20346652974023272 1106 4 0.2414777956278371 1106 5 0.06499710275723203 1106 6 0.032272094498885906 1106 7 0.18835313152160182 1106 8 0.08859878359643858 1106 9 0.1104018366772479 1106 10-11 0.1343568603937939 1106 12-13 0.18808787353984258 1106 14-15 0.24352820102221528 1106 16-17 0.14806986694577517 1106 18-19 0.21180168473952676 1106 20-21 0.3025859233425692 1106 22-23 0.234456260946871 1106 24-25 0.21873946817638767 1106 26-27 0.25845605759338497 1106 28-29 0.3016769595889812 1106 30-31 0.31307152924587456 1106 32-33 0.32587141306886735 1106 34-35 0.4338518024609641 1106 36-37 0.3726285474107698 1106 38-39 0.3607462978456013 1106 40-41 0.4095710954765366 1106 42-43 0.3631339570999259 1106 44-45 0.37480332590430976 1106 46-47 0.35112624516747104 1106 48-49 0.37563937814570636 1106 50-51 0.2623787265626589 1106 52-53 0.3546412340341618 1106 54-55 0.44128640093923366 1106 56-57 0.3948340545966218 1106 58-59 0.3876491388680776 1106 60-61 0.40668943199744234 1106 62-63 0.40553026900502687 1106 64-65 0.39914338418168427 1106 66-67 0.36425457715602505 1106 68-69 0.28684683620472384 1106 70-71 0.3608038795545241 1106 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-2.0793717685139725 2216 82-83 -1.9460300868102074 2216 84-85 -1.9352589783359662 2216 86-87 -1.974295927176943 2216 88-89 -2.011261950899975 2216 90-91 -2.032854971659159 2216 92-93 -2.2147871463083497 2216 94-95 -2.12163195482362 2216 96-97 -2.0471450629298005 2216 98-99 -2.071694093496017 2216 100-101 -2.030665881007817 2216 102-103 -2.097977539605008 2216 104-105 -2.0986987789619462 2216 106-107 -2.065749539213357 2216 108-109 -2.1531021303887634 2216 110-111 -2.1514491984713686 2216 112-113 -2.1295884320601886 2216 114-115 -2.1361328797141326 2216 116-117 -2.282777503759128 2216 118-119 -2.316988898858064 2216 120-121 -2.412251441799807 2216 122-123 -2.4712839185636533 2216 124-125 -2.71189065411118 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 42323.0 3 7297.0 4 4142.0 5 3445.0 6 3583.0 7 4318.0 8 4983.0 9 5585.0 10 6432.0 11 6834.0 12 7514.0 13 7396.0 14 7502.0 15 6703.0 16 6191.0 17 5819.0 18 5541.0 19 5647.0 20 5857.0 21 6572.0 22 7797.0 23 10165.0 24 13360.0 25 16680.0 26 20681.0 27 24794.0 28 29429.0 29 36342.0 30 43288.0 31 52618.0 32 66847.0 33 88022.0 34 120390.0 35 176050.0 36 325701.0 37 648032.0 38 321372.0 39 1535.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.775697360189056 18.17937037152636 12.520549642672634 24.52438262561195 2 15.612333020623042 19.6345109189304 40.34405274400952 24.40910331643704 3 19.356587645230768 23.87201620175432 29.156264206345067 27.615131946669848 4 12.543636611763523 15.841411686018539 37.553965010598546 34.06098669161939 5 13.743738945771922 37.587333351034076 34.89239580902393 13.776531894170073 6 32.888480800557446 36.10427381898434 17.421446232938166 13.585799147520047 7 28.91501464621867 31.794901671090436 21.042689591047747 18.247394091643148 8 25.650805577281442 36.06467364401716 19.88465650982699 18.39986426887441 9 26.022770973418325 16.162030508909115 19.88164687510925 37.93355164256331 10-11 24.90944265066414 26.257050698828365 28.381613880106478 20.451892770401017 12-13 25.326017778337285 23.664981151605435 28.27042375667037 22.738577313386905 14-15 22.298335922468134 25.622878239963264 25.49603588505873 26.582749952509864 16-17 22.66876892204641 28.83322495764025 26.137769977835422 22.36023614247791 18-19 22.49794707832476 27.945421069794207 27.525443292394552 22.031188559486477 20-21 23.118158138559167 27.292228263086432 27.435227756120018 22.15438584223438 22-23 22.891949073521513 27.246239444709673 27.174441091782537 22.687370389986274 24-25 22.76434817147876 27.436186837183197 27.177750054545147 22.621714936792895 26-27 22.605817176452486 27.705019686645482 27.10283445734094 22.58632867956109 28-29 22.4295290531909 27.64518204873201 27.35580034806913 22.56948855000796 30-31 22.667780815659622 27.472210373706083 27.23369391669988 22.626314893934417 32-33 22.44128772163075 27.588827115660553 27.233835651305615 22.73604951140308 34-35 22.86449565974205 27.571161723497994 26.9830207598633 22.581321856896654 36-37 22.85223063482819 27.446942341292953 26.964100174723356 22.736726849155502 38-39 22.56574876471806 27.5518000986968 27.18733457864875 22.695116557936394 40-41 22.87088355139225 27.321115885965213 27.165737118781152 22.642263443861385 42-43 22.609639653110744 27.327707120154916 27.126028945324926 22.93662428140941 44-45 22.656297494735327 27.530170111656954 27.024444714119106 22.78908767948861 46-47 22.619032644734556 27.619724457998085 26.85679651791983 22.904446379347533 48-49 22.629804346757474 27.530675436191203 27.02793119346311 22.811589023588212 50-51 22.599593311298538 27.45141747084349 27.04111841722161 22.907870800636353 52-53 22.800795956210763 27.381410746899576 26.922778598067037 22.895014698822628 54-55 22.616093955351786 27.280719957314194 27.059908018703688 23.043278068630336 56-57 22.494485903659793 27.573602008919423 27.13724790706512 22.79466418035566 58-59 22.613330414287176 27.37968250715011 27.003847551261867 23.003139527300846 60-61 22.771472708044204 27.2755601026687 27.247310140106308 22.705657049180786 62-63 22.64439297137996 27.330729761131643 27.146083600663022 22.878793666825374 64-65 22.500225943535547 27.28289155548847 27.037055671555432 23.17982682942055 66-67 22.458224894636256 27.61242914794905 26.799569879247514 23.12977607816718 68-69 22.355909798511117 27.26192156923044 27.18583264256889 23.196335989689555 70-71 22.25110996492003 27.27315084666712 27.18662739797385 23.289111790438994 72-73 22.540513614354474 27.083532346353017 27.07840654760213 23.29754749169038 74-75 22.311789818228412 27.23915689666218 27.09965695781571 23.349396327293697 76-77 22.411464295681693 27.19972361494138 27.067122152225174 23.321689937151753 78-79 22.448416618515964 27.2233742148329 27.074332258485395 23.253876908165736 80-81 22.274351090334115 27.303060278812048 27.17194341380045 23.250645217053393 82-83 22.35516567920097 27.279098087482684 27.147467057878742 23.2182691754376 84-85 22.448008252702675 27.10900072034092 27.020751968823127 23.422239058133282 86-87 22.331947307732626 26.954750340682764 27.28120013510838 23.432102216476235 88-89 22.302626030892892 27.040505162559715 27.20243356183307 23.454435244714322 90-91 22.318054113379926 27.12500599774252 27.21500707384234 23.34193281503521 92-93 22.337933011915624 26.804876747822348 27.322034877369727 23.5351553628923 94-95 22.161704779845387 26.762420089027017 27.36947330959528 23.706401821532317 96-97 22.312215404227594 26.793601067901495 27.235377311145314 23.658806216725598 98-99 22.15897728979001 26.68556177244628 27.17484801467533 23.980612923088383 100-101 22.026420745329556 26.63155633037082 27.426543796008694 23.915479128290926 102-103 22.206361014231792 26.58462079893937 27.28424670925839 23.92477147757045 104-105 22.087835708296847 26.410488107026286 27.456258819114975 24.04541736556189 106-107 22.007393167197726 26.526670870451053 27.502107129464687 23.963828832886534 108-109 22.049818998731247 26.57743478716346 27.180524030682918 24.19222218342238 110-111 21.98043318046392 26.597694966719175 27.346627034717542 24.075244818099367 112-113 22.02798220520827 26.61078142700631 27.400719289605462 23.960517078179958 114-115 21.8484485470372 26.64037933848602 27.307760815582398 24.203411298894373 116-117 21.72497577470875 26.663675660019866 27.391761491821608 24.21958707344978 118-119 21.68187610536194 26.761026600426668 27.162817586659326 24.394279707552062 120-121 21.79626688032496 26.740274743447014 27.290343918424764 24.173114457803262 122-123 21.821531546969354 26.750803714782762 27.22793369865259 24.199731039595292 124-125 21.74859522436826 26.653411371767017 27.16651655693288 24.431476846931847 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 8964.0 1 4747.0 2 519.0 3 503.5 4 439.0 5 342.5 6 295.0 7 285.5 8 286.5 9 277.0 10 266.0 11 264.5 12 285.0 13 304.5 14 331.0 15 393.5 16 504.5 17 607.5 18 717.0 19 907.5 20 1139.0 21 1493.0 22 2094.0 23 2979.0 24 4292.5 25 6133.0 26 8763.5 27 12343.0 28 15782.0 29 19388.5 30 23880.0 31 28596.5 32 33120.5 33 37227.0 34 42362.5 35 47497.5 36 52323.5 37 57181.5 38 60062.0 39 62278.0 40 65569.5 41 70068.0 42 74148.0 43 78080.0 44 81045.0 45 82748.0 46 83084.5 47 82203.0 48 83877.5 49 84643.0 50 83329.0 51 81571.0 52 78413.0 53 75663.0 54 72363.0 55 66867.5 56 60734.5 57 54824.5 58 49547.0 59 44290.5 60 37321.0 61 29670.5 62 23378.5 63 18208.5 64 14375.5 65 11488.0 66 8679.5 67 7067.5 68 5540.5 69 3482.5 70 2283.5 71 1754.0 72 1355.5 73 1028.0 74 751.5 75 609.5 76 409.5 77 200.0 78 113.5 79 70.5 80 40.0 81 31.5 82 28.5 83 20.0 84 13.0 85 15.5 86 16.0 87 12.5 88 9.5 89 6.5 90 7.0 91 6.0 92 5.0 93 4.5 94 4.5 95 4.5 96 3.5 97 4.0 98 2.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.47088562755617497 2 0.4620298620123359 3 0.45711514396182845 4 0.4541014017610455 5 0.4629108020402571 6 0.5230929155266607 7 0.5184563890639178 8 0.5271266935492471 9 0.5337569263909696 10-11 0.5506107000830402 12-13 0.5583305166435073 14-15 0.5380457133690068 16-17 0.5319023158058723 18-19 0.5412449166282994 20-21 0.5412912818929269 22-23 0.5513293616847653 24-25 0.5459509909879835 26-27 0.55323033753449 28-29 0.534290126934185 30-31 0.5402480634388097 32-33 0.5408508118789662 34-35 0.5395989497340257 36-37 0.4885507933792257 38-39 0.4547273328335158 40-41 0.45324364436543807 42-43 0.4659709095056675 44-45 0.47141882809939045 46-47 0.4754294234896631 48-49 0.4899185686857348 50-51 0.4720911244364882 52-53 0.47229976812731156 54-55 0.4607548172350816 56-57 0.4433910256321092 58-59 0.46212259254159077 60-61 0.4581583624159456 62-63 0.475197597166526 64-65 0.47445575293248704 66-67 0.4828015005654244 68-69 0.49212091875553776 70-71 0.48906081129012735 72-73 0.49981755268369105 74-75 0.4891303591870686 76-77 0.48711347017577533 78-79 0.49828749895098584 80-81 0.49193545769702807 82-83 0.49316413720965496 84-85 0.49059086502283256 86-87 0.4804368720694255 88-89 0.48535159011993306 90-91 0.4704219749099007 92-93 0.4706769838653516 94-95 0.4647190473607269 96-97 0.45310454857155574 98-99 0.41564141475259264 100-101 0.41225675043479026 102-103 0.40054952111636427 104-105 0.4058815265485187 106-107 0.40442102071275465 108-109 0.41784376482239555 110-111 0.4418377892670904 112-113 0.4418377892670904 114-115 0.43418752060356447 116-117 0.4283223146281946 118-119 0.4705378880714693 120-121 0.46629546635805946 122-123 0.42929598518537065 124-125 0.43903269075713086 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2156787.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.91065087026656 #Duplication Level Percentage of deduplicated Percentage of total 1 85.40927502750772 63.12655107640699 2 9.396452864143232 13.889958941212132 3 2.490312761367691 5.521819111896504 4 1.0355009701525009 3.0613820272305516 5 0.5204663486516657 1.9234003292457849 6 0.2891183447017848 1.28213550212658 7 0.18282147322767422 0.9458717855522889 8 0.12302698348455399 0.7274403539159136 9 0.09039218614200868 0.6012850780207936 >10 0.42443267173498483 5.583681529642838 >50 0.028722342766395802 1.4669110938806136 >100 0.009351652833738214 1.0400483018760591 >500 0.0 0.0 >1k 0.0 0.0 >5k 1.2637328599710452E-4 0.8295148689928447 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 8881 0.4117699151562022 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 4.6365264627429596E-5 0.0 0.0 0.0 0.0 6 9.273052925485919E-5 0.0 0.0 0.0 0.0 7 9.273052925485919E-5 0.0 0.0 0.0 0.0 8 9.273052925485919E-5 0.0 0.0 0.0 0.0 9 9.273052925485919E-5 0.0 0.0 0.0 0.0 10-11 9.273052925485919E-5 0.0 0.0 0.0 0.0 12-13 2.0864369082343318E-4 0.0 0.0 0.0 0.0 14-15 2.781915877645776E-4 0.0 0.0 0.0 0.0 16-17 2.781915877645776E-4 0.0 0.0 0.0 0.0 18-19 3.7092211701943677E-4 0.0 0.0 0.0 0.0 20-21 4.6365264627429596E-4 0.0 0.0 0.0 0.0 22-23 5.100179109017256E-4 0.0 0.0 6.95478969411444E-5 0.0 24-25 8.345747632937327E-4 0.0 0.0 2.3182632313714798E-4 0.0 26-27 8.345747632937327E-4 0.0 0.0 6.722963370977291E-4 0.0 28-29 9.273052925485919E-4 0.0 0.0 0.0021096195405480465 0.0 30-31 9.736705571760215E-4 0.0 0.0 0.008090738677486465 0.0 32-33 9.736705571760215E-4 0.0 0.0 0.02158303068406848 0.0 34-35 0.0011127663510583103 0.0 0.0 0.042285121340215795 0.0 36-37 0.0013909579388228879 0.0 0.0 0.07450898025627936 0.0 38-39 0.0016691495265874655 0.0 0.0 0.1236097954967273 0.0 40-41 0.0016691495265874655 0.0 0.0 0.17604890979035018 0.0 42-43 0.0018082453204697542 0.0 0.0 0.2285575719809142 0.0 44-45 0.0020400716436069022 0.0 0.0 0.29819819945131343 0.0 46-47 0.0023414458636851947 0.0 0.0 0.3829307205579411 0.0 48-49 0.002503724289881198 0.0 0.0 0.4668054842689612 0.0 50-51 0.002503724289881198 0.0 0.0 0.5555717833981751 0.0 52-53 0.002526906922194913 0.0 0.0 0.6467954415526429 0.0 54-55 0.0025964548191360574 0.0 0.0 0.7525314275354961 0.0 56-57 0.0027355506130183464 0.0 0.0 0.8848578927821802 0.0 58-59 0.0027355506130183464 0.0 0.0 1.0326471737821121 0.0 60-61 0.0027819158776457758 0.0 0.0 1.1918423098803914 0.0 62-63 0.0027819158776457758 0.0 0.0 1.348765548011927 0.0 64-65 0.0027819158776457758 0.0 0.0 1.5135940637624392 0.0 66-67 0.0027819158776457758 0.0 0.0 1.7123619532202299 0.0 68-69 0.002874646406900635 0.0 0.0 1.9419395610229475 0.0 70-71 0.0029210116715280643 0.0 0.0 2.1978990043986726 0.0 72-73 0.002990559568469209 0.0 0.0 2.4550407620223975 0.0 74-75 0.003013742200782924 0.0 0.0 2.7181172735184327 0.0 76-77 0.003013742200782924 0.0 0.0 3.0185641883041763 0.0 78-79 0.0030369248330966384 0.0 0.0 3.358954778566451 0.0 80-81 0.0037092211701943677 0.0 0.0 3.734420691519376 0.0 82-83 0.0037555864348217975 0.0 0.0 4.122335678024766 0.0 84-85 0.0038714995963903716 0.0 0.0 4.51664907104874 0.0 86-87 0.003987412757958946 0.0 0.0 4.927306219853884 0.0 88-89 0.003987412757958946 0.0 0.0 5.390124291364887 0.0 90-91 0.004242421713409808 0.0 0.0 5.892005098324498 0.0 92-93 0.0043583348749783824 0.0 0.0 6.418714504492099 0.0 94-95 0.0043583348749783824 0.0 0.0 6.944589335896405 0.0 96-97 0.0043583348749783824 0.0 0.0 7.474938415337259 0.0 98-99 0.00459016119811553 0.0 0.0 8.050400897260602 0.0 100-101 0.004798804888938963 0.0 0.0 8.690960210720855 0.0 102-103 0.0049610833151349665 0.0 0.0 9.359686422442271 0.0 104-105 0.005702927549173841 0.0 0.0 10.024749778258123 0.0 106-107 0.006073849666193277 0.0 0.0 10.689349481427698 0.0 108-109 0.006143397563134421 0.0 0.0 11.381119229668947 0.0 110-111 0.006259310724702996 0.0 0.0 12.132329247162563 0.0 112-113 0.006259310724702996 0.0 0.0 12.91963462316863 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2150 0.0 51.208084 1 GTATCAA 3440 0.0 42.73107 1 ATCAACG 3535 0.0 40.071285 3 CAACGCA 3695 0.0 38.180393 5 TCAACGC 3760 0.0 37.678677 4 AACGCAG 3985 0.0 35.401897 6 TATCAAC 4200 0.0 34.573696 2 GTACATG 7045 0.0 33.789692 1 TACATGG 7425 0.0 30.777973 2 ACATGGG 7630 0.0 28.471743 3 ACGCAGA 4885 0.0 28.149727 7 CGCAGAG 4940 0.0 27.113297 8 CATGGGG 4505 0.0 25.237427 4 GCAGAGT 6130 0.0 20.878761 9 GTGGTAT 1400 0.0 20.404148 1 AGTACAT 5250 0.0 20.404146 2 GAGTACA 5530 0.0 20.01673 1 TACCGTA 150 0.0046940683 19.842916 7 GGACCGT 305 1.639255E-7 19.516712 6 CAGAGTA 5735 0.0 19.410646 10-11 >>END_MODULE