FastQCFastQC Report
Thu 26 May 2016
SRR1364101_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364101_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2156787
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42330.1962641651679095No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39720.18416283110015036No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22170.10279179167901142No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31250.071.342321
GTATCAA54800.047.5364271
ATCAACG67250.038.394273
TCAACGC68850.037.50294
CAACGCA68950.037.448515
TATCAAC71250.036.4066472
AACGCAG72200.035.7628066
GTGGTAT14850.033.3178751
CGCAGAG85350.030.1872718
ACGCAGA85250.030.1528847
TGGTATC15200.030.1388172
GTACATG67500.030.0262281
TACATGG70450.028.5439952
ACATGGG71850.027.3247473
GCAGAGT96950.026.2071329
GAGTACT62750.025.74456212-13
CAGAGTA94700.024.22193510-11
AGAGTAC91000.023.50671410-11
CATGGGG41550.023.196534
GTACTTT69350.023.0767614-15
Produced by FastQC (version 0.11.5)