FastQCFastQC Report
Thu 26 May 2016
SRR1364100_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364100_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2376208
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN98110.41288473062964187No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22850.051.304991
GTATCAA36100.047.474991
ATCAACG41400.040.2550163
TCAACGC41350.040.0251244
CAACGCA41600.039.7854355
AACGCAG44550.037.0188646
TATCAAC47000.036.0895732
GTACATG86050.035.546091
TACATGG85600.034.5555152
ACATGGG87150.031.7576123
ACGCAGA54150.029.9081157
CGCAGAG57250.028.288648
CATGGGG62000.026.5021324
TAAGGTG13150.025.3533235
GAGTACA62700.024.3918741
GTAAGGT12900.023.9981124
AGGTAAG11150.022.4186342
GTGTAAG9200.021.99231
GCAGAGT72300.021.2471169
CATGGGA32350.020.7954164
Produced by FastQC (version 0.11.5)