##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364100_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2376208 Sequences flagged as poor quality 0 Sequence length 125 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.99736092126615 31.0 31.0 34.0 28.0 34.0 2 31.05756188010477 33.0 31.0 34.0 27.0 34.0 3 31.12232388747113 33.0 31.0 34.0 27.0 34.0 4 34.60772625965404 37.0 35.0 37.0 32.0 37.0 5 34.6436157945769 37.0 35.0 37.0 32.0 37.0 6 34.65760110226041 37.0 35.0 37.0 32.0 37.0 7 34.579393302269835 37.0 35.0 37.0 32.0 37.0 8 34.553779803788224 37.0 35.0 37.0 31.0 37.0 9 36.0864314066782 39.0 37.0 39.0 31.0 39.0 10-11 35.99143130567694 39.0 37.0 39.0 31.0 39.0 12-13 35.90527070862483 39.0 37.0 39.0 30.0 39.0 14-15 37.170249616195214 40.0 37.0 41.0 31.0 41.0 16-17 37.16696160437134 40.0 37.0 41.0 31.0 41.0 18-19 37.150453579821296 40.0 37.0 41.0 31.0 41.0 20-21 37.15517959707231 40.0 37.0 41.0 31.0 41.0 22-23 37.11487588628605 40.0 37.0 41.0 31.0 41.0 24-25 37.10467160282265 40.0 37.0 41.0 31.0 41.0 26-27 37.05478771218681 40.0 37.0 41.0 30.5 41.0 28-29 37.00631931211409 40.0 37.0 41.0 30.0 41.0 30-31 36.939088455219405 40.0 37.0 41.0 30.0 41.0 32-33 36.84158394382983 40.0 37.0 41.0 30.0 41.0 34-35 36.81207200716436 40.0 37.0 41.0 30.0 41.0 36-37 36.74449206466774 40.0 37.0 41.0 30.0 41.0 38-39 36.652847730501705 40.0 36.0 41.0 30.0 41.0 40-41 36.563367769151526 40.0 36.0 41.0 30.0 41.0 42-43 36.432700335997524 40.0 36.0 41.0 30.0 41.0 44-45 36.325861624908256 40.0 36.0 41.0 29.5 41.0 46-47 36.1995553419566 40.0 35.0 41.0 29.0 41.0 48-49 36.06621768801384 40.0 35.0 41.0 29.0 41.0 50-51 35.91356396409742 39.0 35.0 41.0 28.0 41.0 52-53 35.73893973086531 39.0 35.0 41.0 28.0 41.0 54-55 35.52684592426252 39.0 35.0 41.0 28.0 41.0 56-57 35.298505433867746 38.5 35.0 41.0 28.0 41.0 58-59 35.05018836734831 38.0 34.0 40.0 27.0 41.0 60-61 34.770295992606705 37.5 34.0 40.0 26.5 41.0 62-63 34.47137329728711 37.0 34.0 40.0 26.0 41.0 64-65 34.14750476389273 36.5 34.0 40.0 26.0 41.0 66-67 33.82273710887262 36.0 33.5 39.0 26.0 41.0 68-69 33.48584277975665 35.5 33.0 39.0 26.0 41.0 70-71 33.13113982445981 35.0 33.0 38.5 25.5 40.0 72-73 32.79854415101708 35.0 33.0 37.0 25.0 40.0 74-75 32.54331712543683 35.0 33.0 37.0 25.0 39.0 76-77 32.3488572970043 35.0 33.0 36.5 25.0 39.0 78-79 32.08322377502306 35.0 33.0 36.0 24.5 38.5 80-81 31.927721184340765 35.0 33.0 36.0 24.5 37.0 82-83 31.87963574737565 35.0 33.0 35.0 25.0 37.0 84-85 31.711191949526302 35.0 33.0 35.0 25.0 36.5 86-87 31.557040250685127 35.0 33.0 35.0 24.5 36.0 88-89 31.403500451138957 35.0 33.0 35.0 24.0 36.0 90-91 31.283755252065475 35.0 33.0 35.0 24.0 36.0 92-93 31.181977545736736 35.0 33.0 35.0 23.5 35.5 94-95 31.084625798751624 35.0 33.0 35.0 23.0 35.0 96-97 31.009534518863667 35.0 33.0 35.0 23.0 35.0 98-99 30.935412640644252 35.0 33.0 35.0 20.0 35.0 100-101 30.86027948731761 35.0 32.5 35.0 20.0 35.0 102-103 29.727001592453185 33.5 30.0 34.5 18.5 35.0 104-105 30.572951315709737 35.0 32.0 35.0 19.0 35.0 106-107 30.669770701891416 35.0 32.0 35.0 19.0 35.0 108-109 30.64466073677052 35.0 32.0 35.0 18.0 35.0 110-111 30.55861187236134 35.0 32.0 35.0 17.5 35.0 112-113 30.46343775460734 35.0 32.0 35.0 14.0 35.0 114-115 30.346176555251056 35.0 32.0 35.0 7.5 35.0 116-117 30.237388099021636 35.0 32.0 35.0 2.0 35.0 118-119 30.088120442318182 35.0 32.0 35.0 2.0 35.0 120-121 29.929126364358673 35.0 32.0 35.0 2.0 35.0 122-123 29.751763734487888 35.0 32.0 35.0 2.0 35.0 124-125 28.86168614027055 34.5 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5573197764645634 1101 2 0.5515127545414948 1101 3 0.6377390635606055 1101 4 0.6546513672110166 1101 5 0.6391901099898405 1101 6 0.565279896722636 1101 7 0.5601875055462244 1101 8 0.5219821225328758 1101 9 0.649315650052813 1101 10-11 0.6818574898944902 1101 12-13 0.7415604078479063 1101 14-15 0.8608668946058984 1101 16-17 0.7706391167022346 1101 18-19 0.845078855349783 1101 20-21 0.8148281167785214 1101 22-23 0.8309758161927476 1101 24-25 0.8035515116066136 1101 26-27 0.7660930659093239 1101 28-29 0.8312305230873775 1101 30-31 0.7986592730315536 1101 32-33 0.8410010445303087 1101 34-35 0.8705425955039559 1101 36-37 0.8400613601436149 1101 38-39 0.8400186492961979 1101 40-41 0.937449579370984 1101 42-43 0.8877838069091979 1101 44-45 0.8315895046413857 1101 46-47 0.8279915450043092 1101 48-49 0.7774365737013724 1101 50-51 0.8464487255140014 1101 52-53 0.813089616834823 1101 54-55 0.7765553851670646 1101 56-57 0.8067311237086798 1101 58-59 0.8290380413072853 1101 60-61 0.7816932713355271 1101 62-63 0.8577333038781134 1101 64-65 0.77938627981694 1101 66-67 0.7815969175758184 1101 68-69 0.784511688547127 1101 70-71 0.7694382092183503 1101 72-73 0.76706769224743 1101 74-75 0.7426644328868619 1101 76-77 0.6617388484684525 1101 78-79 0.7061723634552806 1101 80-81 0.7015087378966136 1101 82-83 0.6031295564260866 1101 84-85 0.6640810660475331 1101 86-87 0.6695848165679834 1101 88-89 0.6688132881601554 1101 90-91 0.6335005147312636 1101 92-93 0.6977396219386556 1101 94-95 0.729249181124775 1101 96-97 0.7450733170642003 1101 98-99 0.6856723545874743 1101 100-101 0.7434582152549822 1101 102-103 0.757351255718504 1101 104-105 0.7330271069215506 1101 106-107 0.7449907407271432 1101 108-109 0.8016170352932512 1101 110-111 0.8044012456363276 1101 112-113 0.8372483379035813 1101 114-115 0.8261808903783425 1101 116-117 0.8280244208577265 1101 118-119 0.9239068171574125 1101 120-121 0.9879740616103767 1101 122-123 0.9969231586601026 1101 124-125 1.0954815244138096 1102 1 0.4498024094196964 1102 2 0.5367013754314343 1102 3 0.5546666115833965 1102 4 0.5947130561286116 1102 5 0.5004064846021876 1102 6 0.5036225155450964 1102 7 0.5063555166342937 1102 8 0.5063684100636152 1102 9 0.6367579503658689 1102 10-11 0.5648230032314174 1102 12-13 0.6520780957086956 1102 14-15 0.8000088692803118 1102 16-17 0.7670642627992876 1102 18-19 0.7311366986699994 1102 20-21 0.7524614178904798 1102 22-23 0.7432079611092917 1102 24-25 0.8201741377260632 1102 26-27 0.8768351571678181 1102 28-29 0.8924337295691132 1102 30-31 0.764644272266203 1102 32-33 0.8085007142786296 1102 34-35 0.7533456879178146 1102 36-37 0.85226528164646 1102 38-39 0.7819960076601689 1102 40-41 0.7921551940689682 1102 42-43 0.7038680913324242 1102 44-45 0.8274382724356641 1102 46-47 0.8336471312901637 1102 48-49 0.8614695024152326 1102 50-51 0.8896813338313123 1102 52-53 0.8545982484605616 1102 54-55 0.8565485914181394 1102 56-57 0.8604807908359433 1102 58-59 0.8290863104739401 1102 60-61 0.8127609593011584 1102 62-63 0.8054537285293577 1102 64-65 0.7595044574986289 1102 66-67 0.7521921883717155 1102 68-69 0.7944330362256196 1102 70-71 0.7673797452346491 1102 72-73 0.7279029459331454 1102 74-75 0.7032701249581024 1102 76-77 0.7100009697536436 1102 78-79 0.686912714339833 1102 80-81 0.5996081237333293 1102 82-83 0.6435556335183641 1102 84-85 0.6723787916094359 1102 86-87 0.5850127912969114 1102 88-89 0.566588251517949 1102 90-91 0.5784776906704927 1102 92-93 0.5908298341174998 1102 94-95 0.5915759143170582 1102 96-97 0.6333493613409864 1102 98-99 0.5790014802697314 1102 100-101 0.5985730484831251 1102 102-103 0.6836508223024893 1102 104-105 0.6594989076214723 1102 106-107 0.632961821163871 1102 108-109 0.6096138648770939 1102 110-111 0.6539442402118105 1102 112-113 0.6622056777734535 1102 114-115 0.6397625589985587 1102 116-117 0.650407433130507 1102 118-119 0.5680295743291168 1102 120-121 0.579557068012015 1102 122-123 0.5678906178609147 1102 124-125 0.6105175585419147 1103 1 0.361758656000184 1103 2 0.41698930853557314 1103 3 0.3936147493274582 1103 4 0.4531850345050472 1103 5 0.45614399674910544 1103 6 0.4027700669704686 1103 7 0.4583329948106609 1103 8 0.45643185987130863 1103 9 0.5136463783295113 1103 10-11 0.5289409937425305 1103 12-13 0.5451059254312938 1103 14-15 0.6214224852660735 1103 16-17 0.6023173270901978 1103 18-19 0.5326481433167771 1103 20-21 0.4998686317334844 1103 22-23 0.6125103806946157 1103 24-25 0.6776551721947399 1103 26-27 0.6631920194645886 1103 28-29 0.6910511443773331 1103 30-31 0.6053465481299654 1103 32-33 0.478784850193513 1103 34-35 0.6816585109851232 1103 36-37 0.6881549671738512 1103 38-39 0.7624243142770766 1103 40-41 0.7537062029780728 1103 42-43 0.7957044202261798 1103 44-45 0.8483861815317653 1103 46-47 0.8099834969309114 1103 48-49 0.7617315493044003 1103 50-51 0.7454155387488797 1103 52-53 0.6991730449230431 1103 54-55 0.7395932839274693 1103 56-57 0.715867491690922 1103 58-59 0.7726397038545798 1103 60-61 0.8169953561196834 1103 62-63 0.8297147056727283 1103 64-65 0.8049202293079745 1103 66-67 0.8272489828907013 1103 68-69 0.8524344489433417 1103 70-71 0.8100058486651847 1103 72-73 0.8210638814299287 1103 74-75 0.7808522136571696 1103 76-77 0.7559958333406414 1103 78-79 0.7860548762062507 1103 80-81 0.7796822301689552 1103 82-83 0.730513839938471 1103 84-85 0.6839907596967159 1103 86-87 0.7085993386311245 1103 88-89 0.6417290333306376 1103 90-91 0.6473793638835659 1103 92-93 0.6659538756334342 1103 94-95 0.7195942306515057 1103 96-97 0.6720391268951751 1103 98-99 0.7216925033876755 1103 100-101 0.72456656468038 1103 102-103 0.7037955237944153 1103 104-105 0.7481766283279931 1103 106-107 0.7748448187385719 1103 108-109 0.8493699465044138 1103 110-111 0.7638130534472616 1103 112-113 0.7603154124601623 1103 114-115 0.7821823076491263 1103 116-117 0.7707427290254607 1103 118-119 0.7991962096927914 1103 120-121 0.7673075804284153 1103 122-123 0.7370970869648588 1103 124-125 0.8063457949312394 1104 1 0.30580092996621033 1104 2 0.35218401697955315 1104 3 0.4249310035903271 1104 4 0.38996167868906895 1104 5 0.44789481078951354 1104 6 0.3418909921301321 1104 7 0.41356330949471953 1104 8 0.3726886916422103 1104 9 0.3670731181813238 1104 10-11 0.42417481767257215 1104 12-13 0.4239453227987795 1104 14-15 0.4962317602047861 1104 16-17 0.5199561722390555 1104 18-19 0.5895068876822478 1104 20-21 0.5912817145450262 1104 22-23 0.49942683177972924 1104 24-25 0.5212927332101174 1104 26-27 0.4909569025209066 1104 28-29 0.5999115226908671 1104 30-31 0.5716791942029218 1104 32-33 -0.11883359597643306 1104 34-35 0.475229217138029 1104 36-37 0.6330741165567346 1104 38-39 0.6396894271073421 1104 40-41 0.7072500419124026 1104 42-43 0.6134064254597646 1104 44-45 0.6250121295276898 1104 46-47 0.585635046406523 1104 48-49 0.5736222479472275 1104 50-51 0.5832119812329282 1104 52-53 0.6852218099634086 1104 54-55 0.722061404972834 1104 56-57 0.6974538611642558 1104 58-59 0.6983693986722841 1104 60-61 0.7144202387510248 1104 62-63 0.680148239475507 1104 64-65 0.5719945425471096 1104 66-67 0.6015259719735013 1104 68-69 0.7218770592134334 1104 70-71 0.6488441629712156 1104 72-73 0.6079550082229659 1104 74-75 0.6810773175945997 1104 76-77 0.6721608110340114 1104 78-79 0.6444154129353592 1104 80-81 0.6606041818742447 1104 82-83 0.6797579407421601 1104 84-85 0.6212143451280561 1104 86-87 0.6277791281017358 1104 88-89 0.6268365022049132 1104 90-91 0.6759435637390112 1104 92-93 0.5995551120678115 1104 94-95 0.5318009279356701 1104 96-97 0.5113003584644993 1104 98-99 0.5679602545746789 1104 100-101 0.6029387629526219 1104 102-103 0.5820602087916171 1104 104-105 0.6009717276889717 1104 106-107 0.5983241164073085 1104 108-109 0.6343964394879826 1104 110-111 0.5560202993979892 1104 112-113 0.564315064865621 1104 114-115 0.5554542537567535 1104 116-117 0.5915031935716115 1104 118-119 0.6218819626615364 1104 120-121 0.6536446412057622 1104 122-123 0.6872033554327999 1104 124-125 0.8011321146757169 1105 1 0.3313740751640246 1105 2 0.4330157085802995 1105 3 0.38781547710636133 1105 4 0.3959084075397854 1105 5 0.4393550180830914 1105 6 0.40239395992805527 1105 7 0.3093881674367296 1105 8 0.4058482108383856 1105 9 0.4351752165529206 1105 10-11 0.5556365062751425 1105 12-13 0.46566968397654307 1105 14-15 0.5514034459266881 1105 16-17 0.5876176662303934 1105 18-19 0.5104228082438738 1105 20-21 0.5424558838561708 1105 22-23 0.4547132184418814 1105 24-25 0.5882759639573862 1105 26-27 0.6090155666863595 1105 28-29 0.6111498336089554 1105 30-31 0.6433825124432886 1105 32-33 0.20064238555699632 1105 34-35 0.5202134244977827 1105 36-37 0.5181866983592371 1105 38-39 0.5437336944338256 1105 40-41 0.5819172305935894 1105 42-43 0.6147037305133907 1105 44-45 0.47795293762658986 1105 46-47 0.47623152531340907 1105 48-49 0.47318505305165104 1105 50-51 0.5727416548053057 1105 52-53 0.5611173408789369 1105 54-55 0.5521545681752045 1105 56-57 0.5383446535918992 1105 58-59 0.5445207321673564 1105 60-61 0.48371834723695883 1105 62-63 0.4940536178486781 1105 64-65 0.45062786646376907 1105 66-67 0.505368724066237 1105 68-69 0.474516046740284 1105 70-71 0.45144172641893476 1105 72-73 0.5023689116221348 1105 74-75 0.5610454431650709 1105 76-77 0.5478703665438971 1105 78-79 0.5069459185456964 1105 80-81 0.507804028547941 1105 82-83 0.5339098117142704 1105 84-85 0.5080290406409809 1105 86-87 0.4438561781259125 1105 88-89 0.4582152576053886 1105 90-91 0.4385994394253352 1105 92-93 0.4819587771169438 1105 94-95 0.4225167001499308 1105 96-97 0.3974224105938937 1105 98-99 0.4739378388751554 1105 100-101 0.47968538937963956 1105 102-103 0.4260138549532897 1105 104-105 0.4924983542180392 1105 106-107 0.5048765970354836 1105 108-109 0.5023476778267373 1105 110-111 0.4474694200679288 1105 112-113 0.4791176866279052 1105 114-115 0.5313452928115083 1105 116-117 0.5622613346246972 1105 118-119 0.5376643280645901 1105 120-121 0.574587505200725 1105 122-123 0.5471601253318639 1105 124-125 0.584446722902733 1106 1 0.2926994716676852 1106 2 0.33975466023118983 1106 3 0.30193047000262396 1106 4 0.33994128240618693 1106 5 0.2719410525185495 1106 6 0.24948821480851535 1106 7 0.363817798937923 1106 8 0.2659328334822959 1106 9 0.30673486832043295 1106 10-11 0.365706861732356 1106 12-13 0.35131504005440917 1106 14-15 0.43620290553342755 1106 16-17 0.4713098475921029 1106 18-19 0.4915971139834028 1106 20-21 0.47952722010390403 1106 22-23 0.41532619837637696 1106 24-25 0.46885976843785215 1106 26-27 0.5980320327167803 1106 28-29 0.5341690908541992 1106 30-31 0.5934627575120786 1106 32-33 0.6267702553748862 1106 34-35 0.6105528421263031 1106 36-37 0.5758467163283498 1106 38-39 0.5679095051415715 1106 40-41 0.5818489586484432 1106 42-43 0.471747933965986 1106 44-45 0.4749950889926424 1106 46-47 0.5181239450691209 1106 48-49 0.5208791739965406 1106 50-51 0.4887150744708251 1106 52-53 0.4775498088476837 1106 54-55 0.5110609066085843 1106 56-57 0.5151644034007532 1106 58-59 0.5512372302832347 1106 60-61 0.46471737713111594 1106 62-63 0.45796619896186996 1106 64-65 0.4279999983742826 1106 66-67 0.4058676670688257 1106 68-69 0.4868107320246864 1106 70-71 0.49710819416205254 1106 72-73 0.4142827824387254 1106 74-75 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-2.522860243219398 2216 110-111 -2.4923537548295123 2216 112-113 -2.46675184635437 2216 114-115 -2.581442853844596 2216 116-117 -2.572927931569261 2216 118-119 -2.7291550159389253 2216 120-121 -2.762594630156869 2216 122-123 -2.9531534617216053 2216 124-125 -3.137579510837803 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 49218.0 3 8006.0 4 4475.0 5 3973.0 6 4251.0 7 4826.0 8 5678.0 9 6313.0 10 6911.0 11 7637.0 12 8108.0 13 8113.0 14 8143.0 15 7480.0 16 6782.0 17 6104.0 18 5987.0 19 6063.0 20 6530.0 21 7146.0 22 8569.0 23 11226.0 24 14803.0 25 18966.0 26 23502.0 27 27515.0 28 33303.0 29 40849.0 30 48610.0 31 60035.0 32 77124.0 33 101426.0 34 141501.0 35 214587.0 36 416688.0 37 709548.0 38 255481.0 39 731.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.41495961210026 17.263686493658827 12.233455295502086 25.08789859873883 2 16.55336784585385 19.15550590833831 38.43117424312892 25.85995200267892 3 19.78610394370854 22.933071656785444 28.443288076876083 28.837536322629937 4 13.23295956343202 15.08189665923862 35.461931994255416 36.22321178307394 5 14.46465838866471 37.141459462933334 33.80377685248763 14.590105295914329 6 34.85461677618497 34.48845107890903 16.495902159510322 14.161029985395679 7 30.446938718528614 31.061961727953214 19.937101650129833 18.55399790338834 8 27.64900452109633 34.012767041266954 19.191389693457314 19.146838744179394 9 26.925179439704706 15.37951857186135 18.807531982662766 38.88777000577118 10-11 26.012592365952198 25.2486966884312 27.02999848922078 21.708712456395823 12-13 26.40054426432794 22.538468293746146 27.037113452237737 24.02387398968818 14-15 23.266766709480706 24.428627251941812 24.10480334563029 28.199802692947195 16-17 23.930716962323743 27.612134643867087 24.990326700485667 23.46682169332351 18-19 24.042009710921413 26.57207944006799 26.12184542628465 23.264065422725942 20-21 24.226285863237614 25.877345727936817 26.228800272852798 23.667568135972772 22-23 24.159822966267228 25.796068837141195 26.10718556272812 23.936922633863457 24-25 24.020244572548165 26.04334767535475 26.077709282606236 23.85869846949085 26-27 23.50190346830949 26.34789065325221 25.994197442278598 24.1560084361597 28-29 23.786876995127177 26.583085616171008 25.909335835927216 23.7207015527746 30-31 23.781075433251885 26.158171662340372 25.823168532132396 24.23758437227535 32-33 23.484103482448962 26.274615757639356 25.83174581143879 24.409534948472892 34-35 24.20262850728045 26.28932536785202 25.749799907374516 23.758246217493014 36-37 24.332771706170877 25.903741840969857 25.63859715008048 24.124889302778783 38-39 23.828532075301 26.21875405589022 25.732174058621275 24.220539810187507 40-41 24.09114333751723 25.9815077003336 26.081320967930814 23.846027994218353 42-43 23.825900844382723 26.035891498056845 25.790109130669077 24.348098526891352 44-45 23.773086518988773 26.2462476049048 25.990474799148156 23.99019107695827 46-47 24.185827612693384 26.317184880638795 25.47848187334652 24.0185056333213 48-49 23.867265739186923 26.370412223244738 25.554736980999298 24.207585056569048 50-51 23.916172001900225 26.404813052765018 25.69717657888389 23.981838366450873 52-53 24.296168599872132 25.998695974264518 25.71218240799938 23.992953017863968 54-55 23.86344274458986 26.317448371937672 25.756764127031772 24.062344756440694 56-57 23.736369881109642 26.540639365918096 25.637389696169087 24.08560105680317 58-59 23.714676799653308 25.978321186785543 25.826918300152734 24.480083713408415 60-61 23.575775639928043 26.342966340493085 25.88435740845261 24.19690061112626 62-63 23.69146576300542 26.280943195013062 26.12501680833592 23.902574233645595 64-65 23.48152655409851 26.30599160728451 25.84844738146262 24.36403445715436 66-67 23.42085888429027 26.48906698850899 25.681233161318534 24.408840965882202 68-69 23.76623827012835 25.88216162904593 26.148055636851463 24.203544463974257 70-71 23.709839966742383 25.956674593404045 25.808487036337667 24.5249984035159 72-73 23.65262128998995 26.170160211444088 25.693996522457052 24.483221976108915 74-75 23.381894643891005 26.440060374588032 25.82671444418459 24.35133053733637 76-77 23.671462240512287 26.11258257700738 25.868567810942267 24.34738737153807 78-79 23.56707200374229 26.266358503059113 25.973569239638234 24.193000253560363 80-81 23.7884933432608 26.231352428553635 25.756111218369675 24.224043009815883 82-83 23.653800241619496 26.20402150764561 25.93324103606677 24.20893721466812 84-85 23.372463441736738 26.352420494830408 25.906309769628226 24.368806293804628 86-87 23.660158606351402 26.277795597105953 25.815977812786826 24.24606798375582 88-89 23.484926976662557 26.318786461449978 25.731428051872264 24.4648585100152 90-91 23.3219239624273 26.26571078830367 25.90129449496092 24.51107075430811 92-93 23.50709255243939 26.029253079048935 26.035489823324664 24.42816454518701 94-95 23.33597586627515 25.753026757231556 26.309015688189568 24.601981688303727 96-97 23.514539494084918 25.524950680350116 26.198441598411115 24.762068227153847 98-99 23.25827974651903 25.861444180580783 26.01668447552678 24.863591597373404 100-101 23.19211537466021 25.60236057011191 26.41178341156472 24.79374064366315 102-103 23.01223999129563 25.284164913996815 26.53503772773704 25.16855736697051 104-105 22.888856539623497 25.62049142384118 26.271824397858218 25.218827638677112 106-107 23.069128429541237 25.557090160618007 26.129218253588448 25.244563156252305 108-109 23.08327525940977 25.491233549302123 26.109641595495297 25.315849595792812 110-111 23.08502935085719 25.5875983698618 26.2675482702925 25.059824008988503 112-113 23.06509691397465 25.693706314102855 26.361970688772217 24.879226083150282 114-115 22.936320580260247 25.929703466218058 26.08954132473313 25.04443462878857 116-117 22.76967936806411 25.838931918624663 26.322554167910855 25.06883454540037 118-119 22.642673041400464 25.888116994716405 26.11382891433962 25.355381049543514 120-121 22.602704395096556 26.05385028114936 26.258512806787664 25.08493251696642 122-123 22.648613191502314 26.05162366314907 26.11447214041542 25.1852910049332 124-125 22.659423555062215 26.048022142735768 26.048867543459934 25.24368675874208 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 9785.0 1 5140.0 2 524.5 3 512.0 4 413.0 5 327.5 6 301.5 7 279.0 8 277.5 9 277.0 10 261.0 11 269.5 12 269.5 13 290.5 14 317.5 15 331.0 16 355.0 17 414.5 18 504.5 19 623.0 20 802.0 21 1077.0 22 1539.5 23 2226.0 24 3072.5 25 4534.5 26 6859.0 27 9773.5 28 12392.5 29 15125.5 30 18919.5 31 24006.5 32 31597.0 33 35406.0 34 35611.5 35 38092.5 36 42237.0 37 47265.0 38 50988.5 39 53941.5 40 57061.5 41 60425.0 42 62403.5 43 65690.0 44 70229.0 45 75567.5 46 79226.5 47 81725.0 48 85189.5 49 87071.5 50 89060.0 51 90558.0 52 93615.0 53 98016.5 54 100665.5 55 97887.5 56 92874.0 57 88083.5 58 84392.0 59 77290.5 60 63558.5 61 52348.0 62 44206.0 63 33255.5 64 23512.5 65 17956.0 66 13291.5 67 10156.0 68 7588.0 69 5660.0 70 4295.0 71 2862.0 72 2163.0 73 1750.5 74 1238.5 75 940.5 76 696.0 77 439.5 78 309.0 79 197.0 80 140.5 81 102.5 82 86.5 83 78.0 84 58.0 85 46.0 86 39.0 87 29.0 88 26.0 89 24.5 90 18.0 91 10.5 92 13.0 93 10.5 94 5.0 95 6.0 96 4.0 97 3.0 98 3.5 99 1.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4741588278467205 2 0.46612081097277674 3 0.4606919932935164 4 0.4565677752115976 5 0.4656158046770316 6 0.5270582373260254 7 0.5215873357887862 8 0.5311824554079441 9 0.5361483506494381 10-11 0.5550440028819026 12-13 0.5633134809747296 14-15 0.5423136358433268 16-17 0.5362956441523637 18-19 0.5467745247890757 20-21 0.5483105856053005 22-23 0.5583896695912143 24-25 0.5515300007406759 26-27 0.5590630113188744 28-29 0.5383998370513019 30-31 0.5448386673220527 32-33 0.5465009797122137 34-35 0.5440390740204561 36-37 0.4902769454525866 38-39 0.45538942718819225 40-41 0.45412691144882933 42-43 0.4674464524991078 44-45 0.4721177607347505 46-47 0.4758211402368816 48-49 0.49086611946428926 50-51 0.47228609616666556 52-53 0.4723492219536337 54-55 0.4617861736009642 56-57 0.4453734689892467 58-59 0.4627330604054864 60-61 0.45884030354245087 62-63 0.4759684337398073 64-65 0.47554759516001965 66-67 0.4835014443180058 68-69 0.4937278218068452 70-71 0.48972985529886276 72-73 0.5005874906573835 74-75 0.48972985529886276 76-77 0.4880465009797122 78-79 0.49993519085871274 80-81 0.4934121928720045 82-83 0.49433803774753726 84-85 0.4907188259613637 86-87 0.4807028677624181 88-89 0.4861948112286466 90-91 0.471044622356292 92-93 0.47100253849831325 94-95 0.46515288223926526 96-97 0.45330627621824354 98-99 0.4156412233272508 100-101 0.41370536586022777 102-103 0.403184401365537 104-105 0.40722445173149824 106-107 0.4078977934591585 108-109 0.4196181479062439 110-111 0.44268010207860586 112-113 0.4430378148714254 114-115 0.43653585881370655 116-117 0.4296130641762001 118-119 0.4732961087581558 120-121 0.46692040427437326 122-123 0.42925535138338056 124-125 0.44044965760573146 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2376208.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.38642934633296 #Duplication Level Percentage of deduplicated Percentage of total 1 83.56881441974384 57.14972822872624 2 9.949810075300404 13.608639674479258 3 2.917690706087017 5.985913479788165 4 1.2519330850945718 3.4246093388062637 5 0.6495348570442474 2.220968480461845 6 0.39154796411805315 1.6065940310315856 7 0.2801177691393004 1.3409377819528026 8 0.17152625855193906 0.9384054689202417 9 0.11936745397923326 0.7346802562022239 >10 0.6346895019633642 7.864419003334891 >50 0.0471953717888668 2.2245101198829897 >100 0.017632596116277148 1.9616745401580618 >500 0.0 0.0 >1k 0.0 0.0 >5k 1.399410728492545E-4 0.9389195962554626 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 9811 0.41288473062964187 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.20838579787628E-5 2 0.0 0.0 0.0 0.0 4.20838579787628E-5 3 0.0 0.0 0.0 0.0 4.20838579787628E-5 4 0.0 0.0 0.0 0.0 4.20838579787628E-5 5 0.0 0.0 0.0 0.0 8.41677159575256E-5 6 0.0 0.0 0.0 0.0 1.683354319150512E-4 7 8.41677159575256E-5 0.0 0.0 0.0 1.683354319150512E-4 8 8.41677159575256E-5 0.0 0.0 0.0 1.683354319150512E-4 9 8.41677159575256E-5 0.0 0.0 0.0 2.10419289893814E-4 10-11 8.41677159575256E-5 0.0 0.0 0.0 2.10419289893814E-4 12-13 8.41677159575256E-5 0.0 0.0 0.0 2.945870058513396E-4 14-15 8.41677159575256E-5 0.0 0.0 0.0 4.418805087770094E-4 16-17 1.472935029256698E-4 0.0 0.0 0.0 5.050062957451536E-4 18-19 2.525031478725768E-4 0.0 0.0 0.0 5.050062957451536E-4 20-21 3.366708638301024E-4 0.0 0.0 1.0520964494690701E-4 6.522997986708234E-4 22-23 3.366708638301024E-4 0.0 0.0 1.472935029256698E-4 7.154255856389677E-4 24-25 4.20838579787628E-4 0.0 0.0 2.525031478725768E-4 7.575094436177304E-4 26-27 4.20838579787628E-4 0.0 0.0 6.522997986708234E-4 7.785513726071118E-4 28-29 4.629224377663908E-4 0.0 0.0 0.0021252348279275214 7.995933015964932E-4 30-31 5.050062957451536E-4 0.0 0.0 0.006985920424474625 8.206352305858746E-4 32-33 5.050062957451536E-4 0.0 0.0 0.01986358096597604 9.258448755327817E-4 34-35 5.050062957451536E-4 0.0 0.0 0.03966403614498394 9.88970662500926E-4 36-37 5.470901537239164E-4 0.0 0.0 0.07065879754634274 0.0010100125914903073 38-39 5.470901537239164E-4 0.0 0.0 0.11638290924026853 0.0010100125914903073 40-41 5.470901537239164E-4 0.0 0.0 0.16397975261424924 0.0010100125914903073 42-43 5.470901537239164E-4 0.0 0.0 0.2116818056331769 0.0010100125914903073 44-45 6.312578696814421E-4 0.0 0.0 0.27609115026967335 0.0010310545204796886 46-47 7.154255856389677E-4 0.0 0.0 0.3563661093641634 0.0010731383784584514 48-49 7.575094436177304E-4 0.0 0.0 0.44026028024482705 0.0011783480234053584 50-51 7.575094436177304E-4 0.0 0.0 0.5369479439510346 0.0013045995973416469 52-53 7.575094436177304E-4 0.0 0.0 0.6281857480489923 0.001367725384309791 54-55 8.41677159575256E-4 0.0 0.0 0.7303653552214284 0.001472935029256698 56-57 8.41677159575256E-4 0.0 0.0 0.857058809666494 0.0015991866031929863 58-59 8.41677159575256E-4 0.0 0.0 0.9975978533865723 0.001683354319150512 60-61 8.41677159575256E-4 0.0 0.0 1.1497941257667679 0.001725438177129275 62-63 8.41677159575256E-4 0.0 0.0 1.306156700086861 0.0018937736090443261 64-65 8.41677159575256E-4 0.0 0.0 1.4719250166652078 0.001935857467023089 66-67 9.679287335115444E-4 0.0 0.0 1.6660368115922513 0.0020200251829806145 68-69 0.0010100125914903073 0.0 0.0 1.8916273322874093 0.0020621090409593773 70-71 0.0010100125914903073 0.0 0.0 2.142531293556793 0.0020831509699487587 72-73 0.0010100125914903073 0.0 0.0 2.4002949236767153 0.0022725283308531914 74-75 0.0010100125914903073 0.0 0.0 2.65626998983254 0.002356696046810717 76-77 0.0010100125914903073 0.0 0.0 2.9456596392235026 0.0023987799047894796 78-79 0.0010100125914903073 0.0 0.0 3.283066970568233 0.0024408637627682424 80-81 0.0012625157393628841 0.0 0.0 3.6512376020954393 0.002525031478725768 82-83 0.0012625157393628841 0.0 0.0 4.041754762209369 0.0026091991946832938 84-85 0.0012835576683522655 0.0 0.0 4.427200817436857 0.0026091991946832938 86-87 0.0013045995973416469 0.0 0.0 4.840148673853467 0.0026512830526620565 88-89 0.0013045995973416469 0.0 0.0 5.281966056843508 0.0027144088396302006 90-91 0.0013887673132991724 0.0 0.0 5.786509430150896 0.002735450768619582 92-93 0.0013887673132991724 2.10419289893814E-5 0.0 6.305171937810158 0.0028617023425558706 94-95 0.0013887673132991724 4.20838579787628E-5 0.0 6.822950684451866 0.002945870058513396 96-97 0.0013887673132991724 4.20838579787628E-5 0.0 7.3692412448741855 0.0030721216324496843 98-99 0.001472935029256698 6.31257869681442E-5 0.0 7.939372310841475 0.0031142054904284475 100-101 0.0015571027452142235 8.41677159575256E-5 0.0 8.568020981328235 0.0031142054904284475 102-103 0.0016412704611717492 8.41677159575256E-5 0.0 9.23829058735599 0.0031773312773965916 104-105 0.0019358574670230889 1.262515739362884E-4 0.0 9.903404920781345 0.0032404570643647357 106-107 0.0019358574670230889 1.262515739362884E-4 0.0 10.563595442823187 0.0032404570643647357 108-109 0.0019568993960124704 1.262515739362884E-4 0.0 11.247921057415848 0.003366708638301024 110-111 0.001977941325001852 1.262515739362884E-4 0.0 11.999075838478786 0.0034298344252691685 112-113 0.001977941325001852 1.262515739362884E-4 0.0 12.785959814965693 0.00345087635425855 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2285 0.0 51.30499 1 GTATCAA 3610 0.0 47.47499 1 ATCAACG 4140 0.0 40.255016 3 TCAACGC 4135 0.0 40.025124 4 CAACGCA 4160 0.0 39.785435 5 AACGCAG 4455 0.0 37.018864 6 TATCAAC 4700 0.0 36.089573 2 GTACATG 8605 0.0 35.54609 1 TACATGG 8560 0.0 34.555515 2 ACATGGG 8715 0.0 31.757612 3 ACGCAGA 5415 0.0 29.908115 7 CGCAGAG 5725 0.0 28.28864 8 CATGGGG 6200 0.0 26.502132 4 TAAGGTG 1315 0.0 25.353323 5 GAGTACA 6270 0.0 24.391874 1 GTAAGGT 1290 0.0 23.998112 4 AGGTAAG 1115 0.0 22.418634 2 GTGTAAG 920 0.0 21.9923 1 GCAGAGT 7230 0.0 21.247116 9 CATGGGA 3235 0.0 20.795416 4 >>END_MODULE