FastQCFastQC Report
Thu 26 May 2016
SRR1364100_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364100_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2376208
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41860.1761630294991011No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40400.17001878623420172No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38150.068.411661
GTATCAA64700.051.758611
ATCAACG82150.040.1222423
TCAACGC83250.039.7350274
TATCAAC85500.038.5501982
CAACGCA86650.038.1758885
AACGCAG88150.037.7287526
GTGGTAT17250.034.543111
GTACATG88350.032.7103921
ACGCAGA101900.032.3478857
TACATGG87050.032.054352
CGCAGAG104250.031.7328518
ACATGGG88450.030.2689693
TGGTATC17300.030.2635272
GTAAGGT11600.027.1832164
TAAGGTG12700.026.7026335
GCAGAGT125500.026.07539
GAGTACT75000.025.94160712-13
CAGAGTA110500.025.60345610-11
GAGTACA65700.024.6691461
Produced by FastQC (version 0.11.5)