FastQCFastQC Report
Thu 26 May 2016
SRR1364099_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364099_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1317040
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN54630.4147937799914961No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16700.059.511311
GTATCAA27450.041.62541
TCAACGC28750.037.0635764
ATCAACG28750.037.0550883
GTACATG57600.036.161391
CAACGCA29500.036.121285
AACGCAG30300.034.3817146
TACATGG59800.034.3321382
ACATGGG58050.034.140883
GCTAACG700.002785487634.009063
TATCAAC33300.033.060582
CATGGGA41050.029.2934384
ACGCAGA36000.028.7725857
CGCAGAG36600.028.1382548
GAGTACA42500.025.9049241
GTGGTAT10400.025.1778621
CATGGGG23450.024.1164234
AGTACAT43500.023.940422
ATGGGAG18500.023.8117945
TGGTATC9500.023.8036162
Produced by FastQC (version 0.11.5)