##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364099_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1317040 Sequences flagged as poor quality 0 Sequence length 125 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.942476310514486 31.0 31.0 34.0 28.0 34.0 2 30.989361750592238 33.0 31.0 34.0 27.0 34.0 3 31.070357012695133 33.0 31.0 34.0 27.0 34.0 4 34.504708285245705 37.0 35.0 37.0 31.0 37.0 5 34.57331136487882 37.0 35.0 37.0 32.0 37.0 6 34.61785139403511 37.0 35.0 37.0 31.0 37.0 7 34.5179478223896 37.0 35.0 37.0 31.0 37.0 8 34.44648605964891 37.0 35.0 37.0 30.0 37.0 9 35.92307902569398 39.0 37.0 39.0 30.0 39.0 10-11 35.84382896495171 39.0 37.0 39.0 30.0 39.0 12-13 35.733688802162426 39.0 37.0 39.0 30.0 39.0 14-15 36.98432735528154 40.0 37.0 41.0 30.0 41.0 16-17 37.00657193403389 40.0 37.0 41.0 30.0 41.0 18-19 37.02645591629715 40.0 37.0 41.0 30.0 41.0 20-21 37.060952590657834 40.0 37.0 41.0 30.0 41.0 22-23 37.034313688270664 40.0 37.0 41.0 30.0 41.0 24-25 37.05798760857681 40.0 37.0 41.0 30.0 41.0 26-27 37.04127285427929 40.0 37.0 41.0 30.0 41.0 28-29 37.00736424102533 40.0 37.0 41.0 30.0 41.0 30-31 36.967529839640406 40.0 37.0 41.0 30.0 41.0 32-33 36.87390587985179 40.0 37.0 41.0 30.0 41.0 34-35 36.86004601226994 40.0 37.0 41.0 30.0 41.0 36-37 36.8017087559983 40.0 37.0 41.0 30.0 41.0 38-39 36.72109123489036 40.0 37.0 41.0 30.0 41.0 40-41 36.65143997145113 40.0 36.5 41.0 30.0 41.0 42-43 36.54708702848812 40.0 36.0 41.0 30.0 41.0 44-45 36.46147801129806 40.0 36.0 41.0 30.0 41.0 46-47 36.36459560833384 40.0 36.0 41.0 29.5 41.0 48-49 36.267137292716995 40.0 36.0 41.0 29.0 41.0 50-51 36.155584492498335 40.0 35.5 41.0 29.0 41.0 52-53 36.031541562898624 40.0 35.0 41.0 28.5 41.0 54-55 35.875504920123916 39.5 35.0 41.0 28.0 41.0 56-57 35.70946326611188 39.0 35.0 41.0 28.0 41.0 58-59 35.51898575593756 39.0 35.0 41.0 28.0 41.0 60-61 35.29731708983782 39.0 35.0 41.0 27.5 41.0 62-63 35.05374476097917 38.0 34.0 40.5 27.0 41.0 64-65 34.786424482172144 38.0 34.0 40.0 26.5 41.0 66-67 34.49130929964162 37.0 34.0 40.0 26.0 41.0 68-69 34.17266066330559 37.0 34.0 40.0 26.0 41.0 70-71 33.83319375265747 36.0 34.0 39.0 26.0 41.0 72-73 33.477550795723744 36.0 33.0 39.0 25.5 41.0 74-75 33.187820415477134 35.5 33.0 39.0 25.5 40.0 76-77 32.94960897163336 35.0 33.0 37.5 25.5 39.5 78-79 32.620486469659234 35.0 33.0 37.0 25.0 39.0 80-81 32.377336299580875 35.0 33.0 37.0 25.0 39.0 82-83 32.235624962036084 35.0 33.0 36.0 25.0 38.5 84-85 31.983780674846628 35.0 33.0 36.0 25.0 37.0 86-87 31.75715923586224 35.0 33.0 36.0 25.0 37.0 88-89 31.544027136609365 35.0 33.0 35.0 24.0 36.5 90-91 31.36643761768815 35.0 33.0 35.0 23.5 36.0 92-93 31.219388173479924 35.0 33.0 35.0 23.0 36.0 94-95 31.097006924618842 35.0 33.0 35.0 22.5 36.0 96-97 30.988555396950737 35.0 33.0 35.0 20.0 36.0 98-99 30.893715073194436 35.0 32.5 35.0 20.0 35.0 100-101 30.7995907489522 35.0 32.0 35.0 19.5 35.0 102-103 29.710342130838853 34.0 30.0 35.0 17.5 35.0 104-105 30.497627634695988 35.0 32.0 35.0 18.0 35.0 106-107 30.576466166555306 35.0 32.0 35.0 17.0 35.0 108-109 30.538059967806596 35.0 32.0 35.0 15.5 35.0 110-111 30.44462810544858 35.0 32.0 35.0 9.0 35.0 112-113 30.349749817773187 35.0 32.0 35.0 4.5 35.0 114-115 30.22980964890968 35.0 32.0 35.0 2.0 35.0 116-117 30.1072526271032 35.0 32.0 35.0 2.0 35.0 118-119 29.957483827370467 35.0 32.0 35.0 2.0 35.0 120-121 29.79664057279961 35.0 32.0 35.0 2.0 35.0 122-123 29.61681611796149 35.0 32.0 35.0 2.0 35.0 124-125 28.770893063232705 34.5 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3513911194812849 1101 2 0.3807361490329555 1101 3 0.48709017300087964 1101 4 0.43582816540241964 1101 5 0.5370556285748833 1101 6 0.5130200399423117 1101 7 0.5774042713974126 1101 8 0.3758695413367974 1101 9 0.41943333682690565 1101 10-11 0.4440876277972805 1101 12-13 0.6068610482733661 1101 14-15 0.705034758220755 1101 16-17 0.6278545311968244 1101 18-19 0.6255720519242516 1101 20-21 0.5670379687486715 1101 22-23 0.666410028581609 1101 24-25 0.6981402675905741 1101 26-27 0.7609478927553823 1101 28-29 0.711471751129551 1101 30-31 0.6688938779375206 1101 32-33 0.835631898209364 1101 34-35 0.7545501464042346 1101 36-37 0.750531086532142 1101 38-39 0.7092877668854101 1101 40-41 0.818068649647536 1101 42-43 0.7238750840702792 1101 44-45 0.7203553859908922 1101 46-47 0.7229673942278723 1101 48-49 0.6435439314307558 1101 50-51 0.734894178127675 1101 52-53 0.7168027677353805 1101 54-55 0.724339055229386 1101 56-57 0.6981021546269517 1101 58-59 0.6271224188785993 1101 60-61 0.7565643782531666 1101 62-63 0.766596432266553 1101 64-65 0.8936233514733516 1101 66-67 0.8325161644409249 1101 68-69 0.9344139176208586 1101 70-71 0.7940798381542251 1101 72-73 0.9344529688639014 1101 74-75 0.7080355072772093 1101 76-77 0.7521334750919308 1101 78-79 0.7656970225852149 1101 80-81 0.7312186396605043 1101 82-83 0.6684926170734045 1101 84-85 0.6020915910016669 1101 86-87 0.6968807110275428 1101 88-89 0.6688122098493317 1101 90-91 0.7023538414520907 1101 92-93 0.6438929947432328 1101 94-95 0.6306922263194359 1101 96-97 0.5675812605400807 1101 98-99 0.59471481097194 1101 100-101 0.5461384597500896 1101 102-103 0.5858799026071217 1101 104-105 0.5052231651363819 1101 106-107 0.618737008760256 1101 108-109 0.6225177653874034 1101 110-111 0.5702244522172037 1101 112-113 0.5917328503388148 1101 114-115 0.6534048704994682 1101 116-117 0.6286583576424185 1101 118-119 0.7862237115514006 1101 120-121 0.8367297212092843 1101 122-123 0.894586801537443 1101 124-125 0.9410372599197778 1102 1 0.36876948306347046 1102 2 0.36950977120297424 1102 3 0.4650023901181015 1102 4 0.4348683793965833 1102 5 0.4376253787995239 1102 6 0.3614645167261159 1102 7 0.3543845374579462 1102 8 0.22409457246343578 1102 9 0.24675212668805102 1102 10-11 0.34983976046257226 1102 12-13 0.47789947667286725 1102 14-15 0.44235426416773294 1102 16-17 0.5019819459652908 1102 18-19 0.2933010088181973 1102 20-21 0.39876992627323915 1102 22-23 0.516501214027052 1102 24-25 0.5289303412550197 1102 26-27 0.5756306551835095 1102 28-29 0.5884297261312312 1102 30-31 0.5476848016651417 1102 32-33 0.5391284760120882 1102 34-35 0.6528766422728793 1102 36-37 0.5269728444997526 1102 38-39 0.5800397609534471 1102 40-41 0.5588305649692344 1102 42-43 0.5061982743943432 1102 44-45 0.622125446211399 1102 46-47 0.6048701151230276 1102 48-49 0.6430487349806953 1102 50-51 0.6514109865100366 1102 52-53 0.5331085617937177 1102 54-55 0.5618100941775239 1102 56-57 0.6850598639838879 1102 58-59 0.6256342308298386 1102 60-61 0.5604697602156534 1102 62-63 0.5825519795062704 1102 64-65 0.6210623167062224 1102 66-67 0.6285310313067143 1102 68-69 0.45129766224154366 1102 70-71 0.5095907769427441 1102 72-73 0.5307330184817189 1102 74-75 0.4593230180473782 1102 76-77 0.499260758192996 1102 78-79 0.5073000500112954 1102 80-81 0.37821286939469445 1102 82-83 0.3969456522378394 1102 84-85 0.4446988133047398 1102 86-87 0.45704999602739704 1102 88-89 0.4320875171310057 1102 90-91 0.4564208047168812 1102 92-93 0.4061265126419116 1102 94-95 0.4706253877921718 1102 96-97 0.40753290164710165 1102 98-99 0.3948029934768087 1102 100-101 0.4023108885977251 1102 102-103 0.46030501273419233 1102 104-105 0.3714958690995722 1102 106-107 0.42281274985133876 1102 108-109 0.28228950725463164 1102 110-111 0.2361964001323571 1102 112-113 0.32726762785556573 1102 114-115 0.37124308823114305 1102 116-117 0.42149530489923137 1102 118-119 0.3827405314835346 1102 120-121 0.39712520848259203 1102 122-123 0.2960623425864881 1102 124-125 0.24359998095352964 1103 1 0.34424009422258806 1103 2 0.32232835504885315 1103 3 0.34093951770978137 1103 4 0.21872781449376077 1103 5 0.35330225121914083 1103 6 0.22451313948602092 1103 7 0.23679472174887906 1103 8 0.3325042936266769 1103 9 0.3097228084459047 1103 10-11 0.28608063204629985 1103 12-13 0.15753343635817174 1103 14-15 0.31133261577202376 1103 16-17 0.1483274731119124 1103 18-19 0.2237875188121592 1103 20-21 0.28976406940228827 1103 22-23 0.2754638644729539 1103 24-25 0.33460393789339093 1103 26-27 0.4259921207002648 1103 28-29 0.5271051244492853 1103 30-31 0.4244775859097629 1103 32-33 0.28702039782258026 1103 34-35 0.47916168042443275 1103 36-37 0.5764111609741107 1103 38-39 0.5759775098139812 1103 40-41 0.6399965171767121 1103 42-43 0.5724187570637653 1103 44-45 0.5352008964901174 1103 46-47 0.5187040781970609 1103 48-49 0.4515304668523541 1103 50-51 0.4688384251315014 1103 52-53 0.4472777276555533 1103 54-55 0.4806144905018712 1103 56-57 0.5706368250755105 1103 58-59 0.5607754565368239 1103 60-61 0.5215791769571538 1103 62-63 0.523222215644914 1103 64-65 0.4959712443396995 1103 66-67 0.5152728715050614 1103 68-69 0.5713734005631039 1103 70-71 0.5191207588129885 1103 72-73 0.389683525945415 1103 74-75 0.4498359602072455 1103 76-77 0.43772407791490053 1103 78-79 0.5061179448135391 1103 80-81 0.484317118308887 1103 82-83 0.4898770246207462 1103 84-85 0.4193264755829915 1103 86-87 0.3790474318813857 1103 88-89 0.5482167862364342 1103 90-91 0.5156415641333432 1103 92-93 0.5587920384330403 1103 94-95 0.5640910513920296 1103 96-97 0.6276278428242392 1103 98-99 0.698332365373993 1103 100-101 0.6851274392000732 1103 102-103 0.6047006028548871 1103 104-105 0.670820662810069 1103 106-107 0.6515803218761675 1103 108-109 0.6085646615834612 1103 110-111 0.5874307483930856 1103 112-113 0.4958980688999759 1103 114-115 0.6441817464033655 1103 116-117 0.6405645876725785 1103 118-119 0.7028066683773027 1103 120-121 0.6208031765945563 1103 122-123 0.5807088123674049 1103 124-125 0.4976582785639003 1104 1 0.32899772356764245 1104 2 0.359801033338897 1104 3 0.3694763582098517 1104 4 0.3591572569302244 1104 5 0.3742914777037427 1104 6 0.34295546091507134 1104 7 0.431940887880927 1104 8 0.3529766482029473 1104 9 0.43076393614697395 1104 10-11 0.43304534724506283 1104 12-13 0.34131002569915836 1104 14-15 0.5220965000162536 1104 16-17 0.5943568962860795 1104 18-19 0.5720723708803064 1104 20-21 0.6153713002583601 1104 22-23 0.5381471573643637 1104 24-25 0.6853347016706266 1104 26-27 0.584601301864943 1104 28-29 0.6831183084283978 1104 30-31 0.5636389264593262 1104 32-33 -0.02505706875597724 1104 34-35 0.49589032534787947 1104 36-37 0.46539900699336556 1104 38-39 0.6931806228168398 1104 40-41 0.7694278510647337 1104 42-43 0.772180787310937 1104 44-45 0.8052714179368294 1104 46-47 0.7646952442889798 1104 48-49 0.7558773274975863 1104 50-51 0.7186056722535241 1104 52-53 0.7216624708381758 1104 54-55 0.6869733258813824 1104 56-57 0.7210985853828547 1104 58-59 0.7739053941750953 1104 60-61 0.797198312989174 1104 62-63 0.8716566181254706 1104 64-65 0.8645046604296098 1104 66-67 0.7959357891560259 1104 68-69 0.8327488630591233 1104 70-71 0.8285313322144248 1104 72-73 0.749648024443232 1104 74-75 0.7686258057609976 1104 76-77 0.7443780011385499 1104 78-79 0.7890947471100276 1104 80-81 0.7271880581757131 1104 82-83 0.6268610556330643 1104 84-85 0.5991443932740879 1104 86-87 0.6368035871248843 1104 88-89 0.5004603099544873 1104 90-91 0.4881019493259764 1104 92-93 0.5383158046421386 1104 94-95 0.6060367316858866 1104 96-97 0.5953913016925227 1104 98-99 0.48290553137494996 1104 100-101 0.5566287220162209 1104 102-103 0.5425595974159627 1104 104-105 0.6257540874610079 1104 106-107 0.6275762767916682 1104 108-109 0.6706674099401475 1104 110-111 0.6966801337382158 1104 112-113 0.7417200209742845 1104 114-115 0.7574776876747329 1104 116-117 0.7495484474643845 1104 118-119 0.7777612548128943 1104 120-121 0.7855173052293125 1104 122-123 0.8093479838741686 1104 124-125 0.9236942295199277 1105 1 0.0754405857058984 1105 2 0.09543704136397935 1105 3 0.12034723682489457 1105 4 0.12597828069612405 1105 5 0.01999338331615519 1105 6 0.08812271471177979 1105 7 -0.07903549903803508 1105 8 -0.014626455069858935 1105 9 -0.10813097526881421 1105 10-11 0.022920948900804206 1105 12-13 0.1227895350462731 1105 14-15 0.1056637037225201 1105 16-17 0.11028780649417769 1105 18-19 0.14673989821117317 1105 20-21 0.09563903474292346 1105 22-23 0.006641249716494713 1105 24-25 0.03838955548240364 1105 26-27 0.058549628053491176 1105 28-29 0.0668698757445938 1105 30-31 0.08663065251553803 1105 32-33 -0.28461710061435497 1105 34-35 0.10149997752174755 1105 36-37 0.05907821908766664 1105 38-39 0.14469410770560387 1105 40-41 0.20749120274156496 1105 42-43 0.1723331280575522 1105 44-45 0.06903740217425991 1105 46-47 0.1347474200661125 1105 48-49 0.1426631075421696 1105 50-51 0.1488577800052795 1105 52-53 0.2374230523118186 1105 54-55 0.24151248053085084 1105 56-57 0.15027778129326208 1105 58-59 0.14488787790764235 1105 60-61 0.1452473061128856 1105 62-63 0.15532466599368178 1105 64-65 0.14540473370810503 1105 66-67 0.22066070294368245 1105 68-69 0.2976188268868185 1105 70-71 0.2577231303287064 1105 72-73 0.3563790074522757 1105 74-75 0.33218442405006243 1105 76-77 0.27178593739633783 1105 78-79 0.21996728125670018 1105 80-81 0.19155154606247748 1105 82-83 0.25594211353899254 1105 84-85 0.16776286274570396 1105 86-87 0.10996765826817168 1105 88-89 0.08471784912346436 1105 90-91 0.12738986378593253 1105 92-93 0.1669795306176951 1105 94-95 0.1800286482343587 1105 96-97 0.08302111102516108 1105 98-99 -0.010428433395127001 1105 100-101 0.0385381920285468 1105 102-103 0.06567960064212386 1105 104-105 0.05695746262304624 1105 106-107 0.1439234576919297 1105 108-109 0.13484624237647225 1105 110-111 0.03027134795923203 1105 112-113 0.19080844387347184 1105 114-115 0.20333888514410248 1105 116-117 0.1420338726839674 1105 118-119 0.10744286742804476 1105 120-121 0.11697569593583523 1105 122-123 0.183547345963877 1105 124-125 0.21580456009033 1106 1 0.18624987810755655 1106 2 0.1579741877855554 1106 3 0.1836504192695294 1106 4 0.1593296353689979 1106 5 0.1676284310520515 1106 6 0.19547025753569613 1106 7 0.19573338631887793 1106 8 0.20452207020106528 1106 9 0.15512232576035956 1106 10-11 0.2541033656502165 1106 12-13 0.16275185547188897 1106 14-15 0.2144606992708944 1106 16-17 0.17619904855691715 1106 18-19 0.16375005470766268 1106 20-21 0.19670195813467473 1106 22-23 0.21521747094625 1106 24-25 0.24977677003910514 1106 26-27 0.3357037590453942 1106 28-29 0.1694403435713312 1106 30-31 0.23837575331885574 1106 32-33 0.19862047176146547 1106 34-35 0.3244649990828137 1106 36-37 0.25329120033875085 1106 38-39 0.2236548134398504 1106 40-41 0.27818868538247443 1106 42-43 0.25788130467493886 1106 44-45 0.12018908969972841 1106 46-47 0.14847307124836817 1106 48-49 0.1278192554782649 1106 50-51 0.13521849183270263 1106 52-53 0.06406693153682852 1106 54-55 0.22504954794769816 1106 56-57 0.2916163330694985 1106 58-59 0.31191148586243855 1106 60-61 0.19882825988065633 1106 62-63 0.1495698429616894 1106 64-65 0.2500177060776281 1106 66-67 0.34189126093909294 1106 68-69 0.31511463008346396 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-1.9947030698064943 2216 92-93 -2.110073408321057 2216 94-95 -2.114306042244035 2216 96-97 -2.041721853925541 2216 98-99 -2.0081877303528586 2216 100-101 -2.0824591296662405 2216 102-103 -2.149692637516271 2216 104-105 -2.0311303765347013 2216 106-107 -1.9740132191533313 2216 108-109 -2.0319360900623806 2216 110-111 -1.9284456101851788 2216 112-113 -1.9422399661077137 2216 114-115 -1.8912339483074625 2216 116-117 -2.0632426713255754 2216 118-119 -2.217514336236249 2216 120-121 -2.370251589876858 2216 122-123 -2.4569756432509315 2216 124-125 -2.8153840727916517 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 25879.0 3 4814.0 4 2674.0 5 2314.0 6 2652.0 7 2857.0 8 3285.0 9 3457.0 10 4025.0 11 4471.0 12 4804.0 13 5013.0 14 5061.0 15 4754.0 16 4231.0 17 4058.0 18 3817.0 19 3875.0 20 3824.0 21 4188.0 22 4982.0 23 6564.0 24 8462.0 25 10329.0 26 12778.0 27 14636.0 28 17630.0 29 21877.0 30 25857.0 31 30693.0 32 39400.0 33 51937.0 34 70815.0 35 102359.0 36 183043.0 37 386549.0 38 227377.0 39 1699.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.421456236835496 19.143175592016277 12.578033394440261 23.85733477670796 2 15.430215225852123 20.517094336571308 40.56100303455178 23.491687403024788 3 19.070956679072115 25.15448331519173 29.864765599553923 25.90979440618223 4 12.115452090738508 16.386325151408826 38.46562218730455 33.03260057054812 5 12.756920372992237 39.008796929160766 35.22242530391056 13.011857393936435 6 31.423466276282074 37.143450308778846 18.05503293106725 13.378050483871828 7 27.75400767495321 33.10907347989217 21.65380893213131 17.483109913023302 8 25.25240420395069 36.549319479948494 20.378734158664102 17.819542157436715 9 26.00881525371717 16.38562383874223 20.4777549957939 37.1278059117467 10-11 24.18418842571385 27.37963811268896 28.680676439151014 19.755497022446175 12-13 24.472807736887194 25.045240265898233 28.539354068264316 21.942597928950256 14-15 21.67236217884735 26.928480830749585 25.99176533141163 25.407391658991436 16-17 21.653303634029875 30.059170874937358 26.745441575487998 21.54208391554477 18-19 21.432363962411 29.218580438497614 28.297711779751882 21.0513438193395 20-21 22.38167889979346 28.432257589227266 28.070168741953104 21.115894769026173 22-23 21.98376498936448 28.60111226314399 27.76500616142475 21.65011658606678 24-25 21.701486042348197 28.442957426585387 28.197128689816815 21.6584278412496 26-27 21.59194244296712 28.812378457495342 27.882108256148037 21.7135708433895 28-29 21.472696578565586 28.94598934420567 27.984036537641654 21.59727753958709 30-31 21.690445345427644 28.69324015765764 28.004995698233564 21.611318798681147 32-33 21.39786596188596 28.81279720530631 27.945410031673905 21.84392680113382 34-35 21.80768571599792 28.8269124112973 27.81734963912015 21.548052233584635 36-37 21.78428064681776 28.621144040024387 27.79762872147969 21.796946591678168 38-39 21.522539042298558 28.889699118392326 27.920563777350964 21.66719806195815 40-41 21.811696981877997 28.67072886557559 27.938175351374355 21.579398801172054 42-43 21.529780267731 28.640336021628414 27.932026718174523 21.89785699246606 44-45 21.671987824275924 28.796693634827452 27.86486166897441 21.666456871922215 46-47 21.643954548298154 28.83611264966576 27.588662708639454 21.931270093396627 48-49 21.554971588849796 28.789456857552054 27.799511207658917 21.856060345939234 50-51 21.558829542784427 28.902909969759015 27.755064830439945 21.78319565701661 52-53 21.68898694579317 28.837808260353288 27.695608075504836 21.777596718348708 54-55 21.52934948353971 28.691140652187013 27.8398450580994 21.939664806173877 56-57 21.555168367636835 29.020751416585345 27.632785689961626 21.791294525816195 58-59 21.598918314844372 28.748758026344145 27.74533866547666 21.90698499333482 60-61 21.57461455582147 28.735018449832666 27.93802381792351 21.752343176422354 62-63 21.45836877717414 28.794919584759672 27.881975252506376 21.864736385559816 64-65 21.367607112872236 28.7349083006778 27.769108205543496 22.128376380906463 66-67 21.40550252924077 28.82144246835588 27.605461328938635 22.167593673464715 68-69 21.36576607785095 28.611934439138437 27.840780546762723 22.181518936247897 70-71 21.343011748691833 28.501450465201806 27.854883283712823 22.300654502393535 72-73 21.379891870442936 28.487712376904383 27.807228731786044 22.32516702086664 74-75 21.231393021653492 28.67716010337274 27.753649672630104 22.337797202343666 76-77 21.254113476638352 28.53308826054015 27.83514200411863 22.377656258702867 78-79 21.31699835174898 28.586243208595324 27.787795311641535 22.308963128014163 80-81 21.252343465650917 28.551404400717555 27.76773117425465 22.428520959376876 82-83 21.263613548805044 28.580535973037296 27.812729149800354 22.34312132835731 84-85 21.340058104156373 28.43437339646072 27.759022263086315 22.466546236296594 86-87 21.171104326751962 28.359621559409913 27.91188183058676 22.55739228325136 88-89 21.309818104132333 28.312084172617958 27.890572544710977 22.48752517853873 90-91 21.324930063875122 28.34489971689887 27.862498750800057 22.467671468425948 92-93 21.27464959037185 28.108981914415597 28.00023344112821 22.616135054084342 94-95 21.16865774625378 28.15022025137912 27.970723673294867 22.71039832907223 96-97 21.28454859151277 27.978219018009966 27.905414888196656 22.83181750228061 98-99 21.123190878324927 27.949655112204336 27.872454229407474 23.054699780063263 100-101 21.08747582061239 27.96563000600053 27.93139572917866 23.015498444208422 102-103 21.151688048718754 27.778510503990265 27.99327128624708 23.076530161043905 104-105 20.948644776838574 27.73116483395838 28.062775373219107 23.25741501598394 106-107 20.92205448569623 27.881815762930117 27.991715758889395 23.20441399248426 108-109 20.965297642399072 27.878433664523673 27.793682183088364 23.362586509988894 110-111 20.879943044474196 28.06005345491167 27.77409370129694 23.28590979931719 112-113 20.861102541642847 28.062099811658133 27.89240527326731 23.18439237343171 114-115 20.771802560574955 28.03294215980257 27.793631812871205 23.401623466751268 116-117 20.66401571809911 28.123669076515924 27.83896345343689 23.373351751948082 118-119 20.659848773530676 28.105366468431292 27.759780075838286 23.475004682199746 120-121 20.646466249211883 28.220840877562832 27.743642536447833 23.389050336777455 122-123 20.77154457998264 28.221273162561054 27.5964240082693 23.410758249187005 124-125 20.68393798189486 28.15631124974261 27.548408745986592 23.611342022375936 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 6067.0 1 3344.5 2 752.5 3 902.0 4 808.5 5 618.0 6 525.5 7 496.0 8 484.5 9 497.5 10 449.5 11 392.5 12 412.0 13 439.5 14 445.0 15 489.5 16 564.0 17 668.5 18 816.0 19 1029.0 20 1348.5 21 1872.0 22 2536.0 23 3426.0 24 4686.5 25 6392.5 26 8763.0 27 11500.0 28 13780.5 29 16072.5 30 19140.5 31 22420.5 32 25964.0 33 29030.0 34 32194.5 35 35247.5 36 38382.5 37 41695.5 38 43050.0 39 43589.0 40 44633.0 41 45702.0 42 46673.5 43 48157.0 44 49527.0 45 50642.5 46 50541.5 47 49388.0 48 48360.5 49 47155.5 50 45960.5 51 43797.0 52 41091.5 53 39141.5 54 37272.0 55 33742.5 56 29967.0 57 27053.5 58 24393.5 59 21202.5 60 17398.5 61 14082.0 62 11028.0 63 8220.0 64 6202.0 65 4760.0 66 3384.0 67 2574.5 68 1921.0 69 1239.0 70 878.0 71 678.0 72 522.0 73 438.5 74 325.5 75 234.0 76 188.0 77 109.0 78 55.0 79 34.5 80 20.0 81 14.0 82 13.5 83 10.0 84 7.0 85 4.5 86 6.0 87 5.0 88 3.5 89 4.0 90 5.0 91 3.0 92 1.0 93 2.0 94 2.5 95 1.5 96 1.0 97 0.5 98 0.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4724989370102654 2 0.4660450707647452 3 0.4598949158719553 4 0.4570855858591994 5 0.4652857923829193 6 0.5223075988580453 7 0.516613010994351 8 0.5268632691490008 9 0.5343801251290773 10-11 0.5497175484419607 12-13 0.5592844560529673 14-15 0.5383663366336633 16-17 0.533051387960882 18-19 0.54178308935188 20-21 0.542162728542793 22-23 0.5526028062928993 24-25 0.5455415173419182 26-27 0.5552602806292899 28-29 0.5351773674299946 30-31 0.5400747129927717 32-33 0.5412895584036932 34-35 0.5403404604264107 36-37 0.48889935005770513 38-39 0.45754115288829494 40-41 0.4562883435582822 42-43 0.4686266172629533 44-45 0.47341007106845656 46-47 0.4772823908157687 48-49 0.4907216181740873 50-51 0.4734859989066391 52-53 0.47352396282573045 54-55 0.4629320293992589 56-57 0.44618994107999754 58-59 0.464868189272915 60-61 0.4595532406001336 62-63 0.47690275162485574 64-65 0.47542215878029526 66-67 0.4836603292231063 68-69 0.4913670047986394 70-71 0.4879122881613315 72-73 0.49922553605053754 74-75 0.4892030614104355 76-77 0.48662151491222744 78-79 0.49808661847779867 80-81 0.49208831926137403 82-83 0.4926957419668347 84-85 0.490683654254996 86-87 0.4818000971876329 88-89 0.4859381643685841 90-91 0.4708285245702484 92-93 0.47204336998116986 94-95 0.46707009658021015 96-97 0.4546558950373565 98-99 0.4196151977160906 100-101 0.41676790378424344 102-103 0.4060620786004981 104-105 0.41084553240600136 106-107 0.40947883131871465 108-109 0.4220448885379336 110-111 0.4435324667436069 112-113 0.4449750956690761 114-115 0.4382554819899168 116-117 0.43168772398712263 118-119 0.47261282876753935 120-121 0.4676775192856709 122-123 0.43153586831075746 124-125 0.4411407398408553 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1317040.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 81.01867205642979 #Duplication Level Percentage of deduplicated Percentage of total 1 89.89239690812886 72.82962625466115 2 7.05043332771669 11.42433491268003 3 1.5475131899290788 3.761323909135908 4 0.5615389878086299 1.819805724006677 5 0.25248136631852863 1.0227852509060096 6 0.15313074945756328 0.7443869983234581 7 0.09156548591464925 0.5192959852504626 8 0.07163726507423589 0.46431648688552385 9 0.04591149222887475 0.3347719319261276 >10 0.3067836099683618 4.55712954582027 >50 0.01984402043661342 1.0949239856599695 >100 0.006613294879984978 0.762054228735444 >500 0.0 0.0 >1k 0.0 0.0 >5k 1.50302137984472E-4 0.6652447860090355 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 5463 0.4147937799914961 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 3.796391909129563E-5 0.0 20-21 0.0 0.0 0.0 7.592783818259127E-5 0.0 22-23 0.0 0.0 0.0 1.138917572738869E-4 0.0 24-25 0.0 0.0 0.0 6.453866245520257E-4 0.0 26-27 0.0 0.0 0.0 0.0016324485209257122 0.0 28-29 0.0 0.0 0.0 0.0036825001518556765 0.0 30-31 0.0 0.0 0.0 0.00990858288282816 0.0 32-33 0.0 0.0 0.0 0.024600619571159568 0.0 34-35 0.0 0.0 0.0 0.04787250197412379 0.0 36-37 0.0 0.0 0.0 0.08754479742452773 0.0 38-39 0.0 0.0 0.0 0.14236469659235862 0.0 40-41 0.0 0.0 0.0 0.20284121970479257 0.0 42-43 0.0 0.0 0.0 0.26369738200813947 0.0 44-45 0.0 0.0 0.0 0.3406122820871044 0.0 46-47 0.0 0.0 0.0 0.43567393549170863 0.0 48-49 0.0 0.0 0.0 0.5399987851545891 0.0 50-51 0.0 0.0 0.0 0.6448551296847476 0.0 52-53 0.0 0.0 0.0 0.7473197473121544 0.0 54-55 0.0 0.0 0.0 0.8676653708315617 0.0 56-57 0.0 3.796391909129563E-5 0.0 1.020659964769483 0.0 58-59 0.0 7.592783818259127E-5 0.0 1.1907383222984875 0.0 60-61 0.0 7.592783818259127E-5 0.0 1.3692067059466684 0.0 62-63 0.0 7.592783818259127E-5 0.0 1.5485862236530403 0.0 64-65 7.592783818259127E-5 7.592783818259127E-5 0.0 1.7341158962522019 0.0 66-67 7.592783818259127E-5 7.592783818259127E-5 0.0 1.9548381218489947 0.0 68-69 7.592783818259127E-5 7.592783818259127E-5 0.0 2.221952256575351 0.0 70-71 7.592783818259127E-5 7.592783818259127E-5 0.0 2.513249407762862 0.0 72-73 7.592783818259127E-5 7.592783818259127E-5 0.0 2.799535321630323 0.0 74-75 7.592783818259127E-5 7.592783818259127E-5 0.0 3.090300977950556 0.0 76-77 7.592783818259127E-5 7.592783818259127E-5 0.0 3.4223334143230275 0.0 78-79 7.592783818259127E-5 7.592783818259127E-5 0.0 3.809451497296969 0.0 80-81 7.592783818259127E-5 7.592783818259127E-5 0.0 4.233128834355828 0.0 82-83 7.592783818259127E-5 7.592783818259127E-5 0.0 4.661589625220191 0.0 84-85 7.592783818259127E-5 7.592783818259127E-5 0.0 5.093011601773674 0.0 86-87 7.592783818259127E-5 7.592783818259127E-5 0.0 5.54880641438377 0.0 88-89 7.592783818259127E-5 7.592783818259127E-5 0.0 6.049360687602503 0.0 90-91 7.592783818259127E-5 7.592783818259127E-5 0.0 6.596306869950799 0.0 92-93 7.592783818259127E-5 7.592783818259127E-5 0.0 7.166524934702059 0.0 94-95 7.592783818259127E-5 7.592783818259127E-5 0.0 7.752080422766204 0.0 96-97 7.592783818259127E-5 1.5185567636518253E-4 0.0 8.34211565328312 0.0 98-99 7.592783818259127E-5 1.5185567636518253E-4 0.0 8.974404725748649 0.0 100-101 7.592783818259127E-5 1.5185567636518253E-4 0.0 9.671118568912107 0.0 102-103 7.592783818259127E-5 1.5185567636518253E-4 0.0 10.389661665553058 0.0 104-105 7.592783818259127E-5 1.5185567636518253E-4 0.0 11.116291076960458 0.0 106-107 7.592783818259127E-5 1.5185567636518253E-4 0.0 11.827658992893154 0.0 108-109 7.592783818259127E-5 1.5185567636518253E-4 0.0 12.57091660086254 0.0 110-111 7.592783818259127E-5 1.5185567636518253E-4 0.0 13.38080088683715 0.0 112-113 7.592783818259127E-5 1.5185567636518253E-4 0.0 14.22705460730122 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1670 0.0 59.51131 1 GTATCAA 2745 0.0 41.6254 1 TCAACGC 2875 0.0 37.063576 4 ATCAACG 2875 0.0 37.055088 3 GTACATG 5760 0.0 36.16139 1 CAACGCA 2950 0.0 36.12128 5 AACGCAG 3030 0.0 34.381714 6 TACATGG 5980 0.0 34.332138 2 ACATGGG 5805 0.0 34.14088 3 GCTAACG 70 0.0027854876 34.00906 3 TATCAAC 3330 0.0 33.06058 2 CATGGGA 4105 0.0 29.293438 4 ACGCAGA 3600 0.0 28.772585 7 CGCAGAG 3660 0.0 28.138254 8 GAGTACA 4250 0.0 25.904924 1 GTGGTAT 1040 0.0 25.177862 1 CATGGGG 2345 0.0 24.116423 4 AGTACAT 4350 0.0 23.94042 2 ATGGGAG 1850 0.0 23.811794 5 TGGTATC 950 0.0 23.803616 2 >>END_MODULE