FastQCFastQC Report
Thu 26 May 2016
SRR1364099_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364099_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1317040
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57500.4365850695498998No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56870.4318016157443965No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32570.24729696896069975No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16900.12831804652857923No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32200.073.878161
GTATCAA54950.050.8866841
ATCAACG70100.039.7193873
TCAACGC70550.039.3817064
GTGGTAT13100.039.140481
CAACGCA72500.038.4045375
TATCAAC74100.037.73732
AACGCAG74900.037.4122476
TGGTATC13250.037.2694852
TACATGG52800.032.56532
GTACATG54650.032.4015121
ACGCAGA85700.032.216467
CGCAGAG86350.031.9050398
ACATGGG51850.031.8985353
GCAGAGT95350.028.6439329
GAGTACT72200.027.44401712-13
CAGAGTA94300.026.40732610-11
CATGGGA34950.026.3851284
GAGTACA39800.025.166641
GTACTTT78700.024.9089514-15
Produced by FastQC (version 0.11.5)