FastQCFastQC Report
Thu 26 May 2016
SRR1364098_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364098_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2800989
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN113990.4069633975713578No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24200.050.4122241
GTATCAA38250.044.030371
TCAACGC43700.037.7295274
ATCAACG44000.037.334323
CAACGCA44750.036.8455775
GTACATG90150.034.7230071
AACGCAG48150.034.2438136
TACATGG88750.033.5961952
TATCAAC51400.032.188622
ACATGGG92350.031.2576793
ACGCAGA57400.028.2084357
GTATACG2803.6379788E-1227.6301141
CGCAGAG58500.027.5762638
CATGGGG65950.025.4517334
GAGTACA63800.023.5991951
GTATAGG8900.021.3972171
GTGGTAT17350.020.9231821
GCAGAGT75600.020.5513679
AGAGTAC62250.019.50887910-11
CAGAGTA66100.019.49835210-11
Produced by FastQC (version 0.11.5)