##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364098_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2800989 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.589791677154032 31.0 31.0 34.0 27.0 34.0 2 30.664780190139982 31.0 31.0 34.0 27.0 34.0 3 30.722911086048533 31.0 31.0 34.0 27.0 34.0 4 34.22664673085114 37.0 35.0 37.0 30.0 37.0 5 34.25341263389467 37.0 35.0 37.0 30.0 37.0 6 34.301237527173434 37.0 35.0 37.0 30.0 37.0 7 34.17258261278427 37.0 35.0 37.0 30.0 37.0 8 34.124308592429315 37.0 35.0 37.0 30.0 37.0 9 35.62453726166008 39.0 35.0 39.0 30.0 39.0 10-11 35.53027823386668 39.0 35.0 39.0 30.0 39.0 12-13 35.43116681286503 39.0 35.0 39.0 29.5 39.0 14-15 36.634042654219634 40.0 37.0 41.0 29.5 41.0 16-17 36.62392408538555 40.0 37.0 41.0 29.5 41.0 18-19 36.61523126295748 40.0 36.5 41.0 29.0 41.0 20-21 36.61934855866981 40.0 37.0 41.0 29.0 41.0 22-23 36.56675695620368 40.0 37.0 41.0 29.0 41.0 24-25 36.558290303889095 40.0 37.0 41.0 29.0 41.0 26-27 36.50387541686169 40.0 36.0 41.0 29.0 41.0 28-29 36.44573791614319 40.0 36.0 41.0 29.0 41.0 30-31 36.38744725523735 40.0 36.0 41.0 29.0 41.0 32-33 36.2800559016833 40.0 36.0 41.0 29.0 41.0 34-35 36.25289138943423 40.0 36.0 41.0 29.0 41.0 36-37 36.19318694218364 40.0 36.0 41.0 29.0 41.0 38-39 36.10199468830474 40.0 36.0 41.0 28.0 41.0 40-41 36.01758307512097 40.0 35.5 41.0 28.0 41.0 42-43 35.89850049393267 40.0 35.0 41.0 28.0 41.0 44-45 35.80137551414875 40.0 35.0 41.0 27.0 41.0 46-47 35.684128891616496 40.0 35.0 41.0 27.0 41.0 48-49 35.559641255285186 39.0 35.0 41.0 27.0 41.0 50-51 35.42150701055948 39.0 35.0 41.0 26.5 41.0 52-53 35.26551335974543 39.0 35.0 41.0 26.0 41.0 54-55 35.07231106584139 39.0 34.0 41.0 26.0 41.0 56-57 34.86612907083891 38.5 34.0 41.0 26.0 41.0 58-59 34.63751607021663 38.0 34.0 40.0 26.0 41.0 60-61 34.37709073473691 38.0 34.0 40.0 25.0 41.0 62-63 34.09794540428399 37.0 34.0 40.0 24.5 41.0 64-65 33.79774322569635 37.0 33.0 40.0 24.0 41.0 66-67 33.48224752042939 36.0 33.0 39.5 23.0 41.0 68-69 33.15250077740398 36.0 33.0 39.0 23.0 41.0 70-71 32.807661508131595 35.0 32.5 39.0 22.0 40.5 72-73 32.474381191786186 35.0 32.0 37.5 22.0 40.0 74-75 32.21539481233236 35.0 32.0 37.0 21.5 39.5 76-77 32.008702640388805 35.0 32.0 37.0 22.0 39.0 78-79 31.7275751529192 35.0 32.0 36.0 21.0 39.0 80-81 31.549171381965444 35.0 32.0 36.0 20.5 37.5 82-83 31.47047899866797 35.0 32.0 36.0 21.0 37.0 84-85 31.28119549916119 35.0 32.0 35.0 20.0 37.0 86-87 31.11013359923941 35.0 32.0 35.0 20.0 36.0 88-89 30.943271644408455 35.0 32.0 35.0 20.0 36.0 90-91 30.810816286675884 35.0 32.0 35.0 19.0 36.0 92-93 30.70273803288767 35.0 32.0 35.0 19.0 36.0 94-95 30.60909593004471 35.0 32.0 35.0 18.0 35.0 96-97 30.527926921526646 35.0 32.0 35.0 17.5 35.0 98-99 30.45264761839479 35.0 32.0 35.0 17.0 35.0 100-101 30.381932060425797 35.0 32.0 35.0 14.0 35.0 102-103 29.27411585693482 33.5 29.5 34.5 10.0 35.0 104-105 30.110975801761448 34.5 31.5 35.0 10.0 35.0 106-107 30.21757600618924 35.0 32.0 35.0 7.0 35.0 108-109 30.208321596407554 35.0 32.0 35.0 6.0 35.0 110-111 30.1353884288728 35.0 32.0 35.0 2.0 35.0 112-113 30.05685295443859 35.0 32.0 35.0 2.0 35.0 114-115 29.956889512954174 35.0 32.0 35.0 2.0 35.0 116-117 29.868327222991596 35.0 32.0 35.0 2.0 35.0 118-119 29.735141944506033 35.0 31.0 35.0 2.0 35.0 120-121 29.595655498825593 35.0 31.0 35.0 2.0 35.0 122-123 29.43228195469529 35.0 31.0 35.0 2.0 35.0 124-125 28.576664885153065 34.5 29.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.41089315025004325 1101 2 0.5288150469551667 1101 3 0.562088952913566 1101 4 0.5548224740074943 1101 5 0.4083635318037224 1101 6 0.5225353592864366 1101 7 0.5417724068992129 1101 8 0.4981079031413387 1101 9 0.45323740305060056 1101 10-11 0.5633869592919325 1101 12-13 0.6288923946497604 1101 14-15 0.704565284294496 1101 16-17 0.6927909208316336 1101 18-19 0.6329578251838299 1101 20-21 0.7180965576882343 1101 22-23 0.7460294979621693 1101 24-25 0.7632894354586739 1101 26-27 0.7196072397966091 1101 28-29 0.7020266865231761 1101 30-31 0.7293943187197627 1101 32-33 0.736507617399738 1101 34-35 0.7449080537189587 1101 36-37 0.7110036193377454 1101 38-39 0.712413310529584 1101 40-41 0.766026578037291 1101 42-43 0.7089032234618884 1101 44-45 0.6985923699034089 1101 46-47 0.7429001479699693 1101 48-49 0.7233621109630235 1101 50-51 0.686037906999239 1101 52-53 0.7375983655910048 1101 54-55 0.6891133514955783 1101 56-57 0.6311855967743654 1101 58-59 0.6735645571971389 1101 60-61 0.6550047335197817 1101 62-63 0.5649050484874039 1101 64-65 0.6237182918241615 1101 66-67 0.6638460569996738 1101 68-69 0.6783663202696175 1101 70-71 0.5642155703111129 1101 72-73 0.6284904158554383 1101 74-75 0.6624593549302773 1101 76-77 0.6824720203578281 1101 78-79 0.6534079308247094 1101 80-81 0.6456759315790741 1101 82-83 0.6314746868720142 1101 84-85 0.6669203809630169 1101 86-87 0.6276783506906867 1101 88-89 0.6110086551101226 1101 90-91 0.6408955450306948 1101 92-93 0.7115861797190703 1101 94-95 0.7070045194092742 1101 96-97 0.6412641655207381 1101 98-99 0.6257665780455 1101 100-101 0.6302687275912007 1101 102-103 0.676404841847436 1101 104-105 0.6077237667445488 1101 106-107 0.6846363720119868 1101 108-109 0.6754548565624745 1101 110-111 0.6936688613098241 1101 112-113 0.7106941235739725 1101 114-115 0.7051515348244379 1101 116-117 0.7550733799221909 1101 118-119 0.8146762708638313 1101 120-121 0.8321887478048104 1101 122-123 0.8381411509035388 1101 124-125 0.8482322563251152 1102 1 0.5037153612678189 1102 2 0.5041157884391723 1102 3 0.38927814891551904 1102 4 0.47406657430432375 1102 5 0.5072974146169429 1102 6 0.5369779482141439 1102 7 0.5096673465396577 1102 8 0.5225886916119293 1102 9 0.6061168077518886 1102 10-11 0.5601107403496783 1102 12-13 0.577703892650618 1102 14-15 0.7283496301838142 1102 16-17 0.7004459670543994 1102 18-19 0.6388325900582146 1102 20-21 0.6401073537681299 1102 22-23 0.612692127923431 1102 24-25 0.6767314925726282 1102 26-27 0.7077154272720776 1102 28-29 0.7621601783748275 1102 30-31 0.7797818563011916 1102 32-33 0.7297030240504014 1102 34-35 0.7899974431777963 1102 36-37 0.7449407229206599 1102 38-39 0.7831410747287606 1102 40-41 0.7765108113479897 1102 42-43 0.7914921367502927 1102 44-45 0.8342184354647131 1102 46-47 0.8446916633364268 1102 48-49 0.8482895486881077 1102 50-51 0.889868740430849 1102 52-53 0.8878083602185285 1102 54-55 0.8401307703784511 1102 56-57 0.969735789629631 1102 58-59 0.9677984340384853 1102 60-61 0.906428157356828 1102 62-63 0.9499688725149866 1102 64-65 0.8403686314591425 1102 66-67 0.8757026426156145 1102 68-69 0.8723170676950076 1102 70-71 0.917556850630973 1102 72-73 0.8233064765532987 1102 74-75 0.759652907579806 1102 76-77 0.8097783140861452 1102 78-79 0.7155078194327054 1102 80-81 0.707554494505299 1102 82-83 0.6477388391148118 1102 84-85 0.6762414553543437 1102 86-87 0.6501376812995758 1102 88-89 0.6079493536342646 1102 90-91 0.6518594420793988 1102 92-93 0.668989067704544 1102 94-95 0.72684731080437 1102 96-97 0.7724550396387997 1102 98-99 0.7643865981414173 1102 100-101 0.7816839116696812 1102 102-103 0.7796700596584643 1102 104-105 0.7566836580285283 1102 106-107 0.759252764396166 1102 108-109 0.7579315682933014 1102 110-111 0.7739659739176581 1102 112-113 0.8391376536972501 1102 114-115 0.9380966346882609 1102 116-117 0.8990921885296217 1102 118-119 0.8196568334064587 1102 120-121 0.8545921578449764 1102 122-123 0.8832346158822588 1102 124-125 0.9761024134777152 1103 1 0.2296090783751481 1103 2 0.32680123276124107 1103 3 0.33939187928760006 1103 4 0.3277200904105584 1103 5 0.3566511575066116 1103 6 0.2613462295208464 1103 7 0.3606399732535337 1103 8 0.3218391072333944 1103 9 0.38406160314674764 1103 10-11 0.41394480269401157 1103 12-13 0.4022033186807903 1103 14-15 0.5257464307055173 1103 16-17 0.5464667745321137 1103 18-19 0.4592959716088245 1103 20-21 0.42328518556867323 1103 22-23 0.483134803804937 1103 24-25 0.4556044977668705 1103 26-27 0.5164650779002926 1103 28-29 0.5522156735895578 1103 30-31 0.5446309377431007 1103 32-33 0.21922792921864698 1103 34-35 0.549020310216072 1103 36-37 0.5728143845793454 1103 38-39 0.663870467772739 1103 40-41 0.6625277168680199 1103 42-43 0.6344723381514257 1103 44-45 0.6358509555039191 1103 46-47 0.6396419088782537 1103 48-49 0.5743954287912274 1103 50-51 0.531788428468964 1103 52-53 0.49905907893754176 1103 54-55 0.5990912770000847 1103 56-57 0.6311862468846741 1103 58-59 0.5930357184664317 1103 60-61 0.6178734395174672 1103 62-63 0.6365454978306389 1103 64-65 0.6112074878034903 1103 66-67 0.6283899347321338 1103 68-69 0.6191352577390816 1103 70-71 0.5820084393161622 1103 72-73 0.5981721966115856 1103 74-75 0.5652373900433787 1103 76-77 0.575695221754529 1103 78-79 0.5583249101131678 1103 80-81 0.5563417550526673 1103 82-83 0.5052111568575626 1103 84-85 0.44936145415211115 1103 86-87 0.4661937132848344 1103 88-89 0.47064187587685424 1103 90-91 0.4298427596493326 1103 92-93 0.44488086399462645 1103 94-95 0.4835464916684167 1103 96-97 0.48251056025276284 1103 98-99 0.5292993099617895 1103 100-101 0.4394463998127378 1103 102-103 0.4402801461396919 1103 104-105 0.45775949030594276 1103 106-107 0.4373329483035704 1103 108-109 0.4549998508593802 1103 110-111 0.4652135981630501 1103 112-113 0.48117682397616335 1103 114-115 0.4656733263594184 1103 116-117 0.42676076722963074 1103 118-119 0.5537069596459503 1103 120-121 0.5559524086539653 1103 122-123 0.5686472682790011 1103 124-125 0.632337693165109 1104 1 0.23763834148001095 1104 2 0.2127723873577274 1104 3 0.22133360270514046 1104 4 0.19349430855043437 1104 5 0.2126976742851454 1104 6 0.139878967640108 1104 7 0.2090955235585028 1104 8 0.23075289123294596 1104 9 0.21452277399798447 1104 10-11 0.19074620184862567 1104 12-13 0.1718554632915641 1104 14-15 0.26679710690355307 1104 16-17 0.2463299866554678 1104 18-19 0.33214886145841405 1104 20-21 0.3385527183136219 1104 22-23 0.218042962290518 1104 24-25 0.3769852077059994 1104 26-27 0.4426306209337696 1104 28-29 0.4223852255624294 1104 30-31 0.3771844963495212 1104 32-33 -0.358491584947906 1104 34-35 0.2902052179916268 1104 36-37 0.3796177832359504 1104 38-39 0.5143732307491646 1104 40-41 0.46743076068546685 1104 42-43 0.48407791258081545 1104 44-45 0.4860506883654878 1104 46-47 0.46833809490065903 1104 48-49 0.5012346653126443 1104 50-51 0.47087094063001445 1104 52-53 0.4618601142076386 1104 54-55 0.5637437662455227 1104 56-57 0.48245843519884346 1104 58-59 0.600187508610631 1104 60-61 0.5325290646278233 1104 62-63 0.5708745355465084 1104 64-65 0.6048606731488348 1104 66-67 0.5303890848402872 1104 68-69 0.5457412707755864 1104 70-71 0.5570923883864012 1104 72-73 0.4903510183253559 1104 74-75 0.5281562531091666 1104 76-77 0.5531124923598014 1104 78-79 0.509295765612304 1104 80-81 0.5120420226671776 1104 82-83 0.4843462190770573 1104 84-85 0.5262498606214727 1104 86-87 0.4643170067999307 1104 88-89 0.4258317633645028 1104 90-91 0.4625899863752636 1104 92-93 0.512699428025158 1104 94-95 0.49214812774125605 1104 96-97 0.4819361174069563 1104 98-99 0.4785182726093957 1104 100-101 0.47386412144438594 1104 102-103 0.4552565402179347 1104 104-105 0.5206217836105296 1104 106-107 0.5009619243577745 1104 108-109 0.4300981855805688 1104 110-111 0.47257925843441484 1104 112-113 0.5216012793343836 1104 114-115 0.5433163585400145 1104 116-117 0.5328492507812435 1104 118-119 0.5214220401341869 1104 120-121 0.48724152028930945 1104 122-123 0.5889001949310178 1104 124-125 0.6510718987035631 1105 1 0.35898388660123715 1105 2 0.3479949893817924 1105 3 0.2800633419379892 1105 4 0.27588353776847896 1105 5 0.34074023325452885 1105 6 0.3116806873737943 1105 7 0.23103020628233395 1105 8 0.32933929783873594 1105 9 0.3605878533983784 1105 10-11 0.45056580301996263 1105 12-13 0.5218373107088183 1105 14-15 0.5804251324119889 1105 16-17 0.5075208563845948 1105 18-19 0.526125115052281 1105 20-21 0.4948767101019129 1105 22-23 0.4164222048849169 1105 24-25 0.5747763107779349 1105 26-27 0.5661126293954126 1105 28-29 0.48531870164894997 1105 30-31 0.530286872549631 1105 32-33 0.15538651162175654 1105 34-35 0.4777166153391903 1105 36-37 0.4772160904920071 1105 38-39 0.534712336797881 1105 40-41 0.5797775735764219 1105 42-43 0.5359877493468872 1105 44-45 0.5414900900330721 1105 46-47 0.5301067802606738 1105 48-49 0.49951146953485903 1105 50-51 0.5948635294690305 1105 52-53 0.645413292022063 1105 54-55 0.5799992689421032 1105 56-57 0.5599094029519094 1105 58-59 0.5833566023101824 1105 60-61 0.5146263138149578 1105 62-63 0.5935370370306288 1105 64-65 0.6027699551486947 1105 66-67 0.5898840708390978 1105 68-69 0.5518748891716996 1105 70-71 0.6285124048422546 1105 72-73 0.5723998606251541 1105 74-75 0.5602870523447194 1105 76-77 0.6198609218910747 1105 78-79 0.5747318756947273 1105 80-81 0.537779598700606 1105 82-83 0.6164713937150914 1105 84-85 0.5493473587048499 1105 86-87 0.5784067691383328 1105 88-89 0.49554407507824294 1105 90-91 0.4798556813415935 1105 92-93 0.5555418625672139 1105 94-95 0.5528653460586064 1105 96-97 0.45484484318505736 1105 98-99 0.4657635880266362 1105 100-101 0.4775134039807085 1105 102-103 0.48551344396953056 1105 104-105 0.5277879471131115 1105 106-107 0.5016154069401324 1105 108-109 0.4456143811984532 1105 110-111 0.4631175625673194 1105 112-113 0.49102727720140393 1105 114-115 0.5542565645978215 1105 116-117 0.5478809096757118 1105 118-119 0.533081175800934 1105 120-121 0.5683831493183042 1105 122-123 0.5255812103860826 1105 124-125 0.6107731649969637 1106 1 0.3154030076492589 1106 2 0.3319413404619418 1106 3 0.3873400663377673 1106 4 0.4132901117861181 1106 5 0.3374262918644888 1106 6 0.2845362250153656 1106 7 0.35475089599685106 1106 8 0.2419609497037527 1106 9 0.4089803294665586 1106 10-11 0.38929375692694634 1106 12-13 0.41040520005364556 1106 14-15 0.516504672917641 1106 16-17 0.42950500765969934 1106 18-19 0.4494974655963162 1106 20-21 0.48771627296022757 1106 22-23 0.5453741055948456 1106 24-25 0.6105227784779146 1106 26-27 0.5268111099275785 1106 28-29 0.5708068341618358 1106 30-31 0.5605521240545457 1106 32-33 0.5913120687728082 1106 34-35 0.6554404869188701 1106 36-37 0.6103509857173464 1106 38-39 0.5765552942580427 1106 40-41 0.6111539123790735 1106 42-43 0.6548010576473189 1106 44-45 0.5491980470081046 1106 46-47 0.5879700772485918 1106 48-49 0.5382123905738609 1106 50-51 0.5611290599071097 1106 52-53 0.5212678943612374 1106 54-55 0.5942666687180491 1106 56-57 0.5544176818931064 1106 58-59 0.5666706028230735 1106 60-61 0.5546526160934135 1106 62-63 0.5327130049007422 1106 64-65 0.6311092005911974 1106 66-67 0.6082421912518612 1106 68-69 0.6081278802864887 1106 70-71 0.6263717353203049 1106 72-73 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-3.5069165467147307 2216 116-117 -3.5586460930995507 2216 118-119 -3.7434007043734923 2216 120-121 -3.6924802779925443 2216 122-123 -3.733979495161101 2216 124-125 -4.0432666221165725 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 72211.0 3 12210.0 4 7076.0 5 5825.0 6 5929.0 7 6986.0 8 8116.0 9 8783.0 10 9741.0 11 10397.0 12 11198.0 13 11303.0 14 11142.0 15 10248.0 16 8895.0 17 8121.0 18 8177.0 19 8235.0 20 8636.0 21 9308.0 22 11030.0 23 14357.0 24 18719.0 25 24117.0 26 29955.0 27 35204.0 28 41911.0 29 50312.0 30 59961.0 31 72883.0 32 91389.0 33 119291.0 34 161676.0 35 238200.0 36 451027.0 37 801440.0 38 335208.0 39 1772.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.65309434044829 17.981999134139198 12.460074096737527 24.904832428674986 2 16.04871676975595 19.23116650969551 39.21608149779894 25.504035222749604 3 19.10147618878547 23.256222633699252 29.222125509579396 28.420175667935887 4 12.70071359254289 15.178921728602447 36.55911973278115 35.561244946073515 5 13.867969393896137 37.49739079162844 34.595771755418006 14.038868059057414 6 34.300408539421326 35.36037167613131 16.60714183179545 13.732077952651917 7 29.546341897542412 31.70101871934476 20.63333711327924 18.119302269833593 8 26.759162726432617 35.22065011618831 19.47534923782266 18.54483791955642 9 26.34134891635122 15.312346457518103 19.460994781215536 38.88530984491514 10-11 25.447756425673546 25.718291263697253 27.790333615959344 21.043618694669856 12-13 25.744273086120945 23.02741627471883 27.726805462540927 23.501505176619297 14-15 22.707873016419946 24.958795803776145 24.82619222977872 27.507138950025194 16-17 23.224822366042147 28.00842701417931 25.73813986582456 23.028610753953977 18-19 23.18048603645722 27.148156589812295 26.91120439482341 22.760152978907072 20-21 23.67927933166533 26.472920827022694 26.895125736301136 22.95267410501084 22-23 23.54831588129503 26.405645916869187 26.63948049523823 23.406557706597553 24-25 23.23546879085903 26.59507813435243 26.777283039849785 23.392170034938758 26-27 23.002230866803664 26.847079276810742 26.67848540854442 23.47220444784117 28-29 23.136517445933684 27.072027644741524 26.559610768470982 23.231844140853806 30-31 23.163671975878565 26.725804937744734 26.536398320285983 23.574124766090723 32-33 22.968044413129395 26.89419649442951 26.51266698085407 23.62509211158702 34-35 23.566584178716674 26.80279900296601 26.41321707132662 23.21739974699069 36-37 23.599009063613185 26.503135735002186 26.35888277680308 23.53897242458155 38-39 23.163084526085132 26.84577369574339 26.428827594616212 23.562314183555266 40-41 23.509386935394218 26.528716670551738 26.630995714465026 23.33090067958902 42-43 23.269258117569883 26.59755995428434 26.48865079793487 23.644531130210915 44-45 23.265483049923386 26.8475031133965 26.577645526178355 23.30936831050176 46-47 23.552527956734878 26.9013099593968 26.100975506257633 23.445186577610684 48-49 23.284890895993158 26.8813732496434 26.260263927514643 23.5734719268488 50-51 23.326643782304433 26.969436104923727 26.35363872120175 23.35028139157009 52-53 23.565941939313724 26.71596821314624 26.34243143247087 23.375658415069164 54-55 23.1917180953269 26.858268500195916 26.497898769746946 23.45211463473024 56-57 23.18279573748242 27.048824239729413 26.399424110100394 23.368955912687774 58-59 23.19957836718064 26.67785186757946 26.435043583549007 23.68752618169089 60-61 22.99531617604866 26.981784730590462 26.549033109542663 23.47386598381821 62-63 23.156891131641512 26.941034003337343 26.56907990547534 23.3329949595458 64-65 22.946241229973026 26.910412691432256 26.49173632499932 23.651609753595398 66-67 22.91268032265262 26.981656898554405 26.429936125226984 23.67572665356599 68-69 23.158106405450862 26.61542612119786 26.720991355310513 23.505476118040765 70-71 23.047235220394736 26.584786999081068 26.517736817814182 23.850240962710018 72-73 23.093151280124413 26.74429138798649 26.427697910838248 23.734859421050846 74-75 22.85825242021844 27.066966103488667 26.46031953879076 23.614461937502128 76-77 23.037788455536433 26.741047053328444 26.577101923646772 23.64406256748835 78-79 23.099453233517668 26.74235062407513 26.600577747345056 23.55761839506215 80-81 23.17091786810486 26.79348429542563 26.417881827380718 23.61771600908879 82-83 22.967744736146294 26.81294372199634 26.644971099883435 23.574340441973927 84-85 23.012884111162794 26.790276547301445 26.55800128111047 23.638838060425293 86-87 23.042404758607972 26.77952768333533 26.54067537968495 23.637392178371748 88-89 22.96369744347966 26.812961952188623 26.481901635427562 23.74143896890415 90-91 22.88021558458002 26.924865524168347 26.53022135045604 23.66469754079559 92-93 22.968402189687293 26.56966805333471 26.75792276696588 23.704006990012118 94-95 22.754530105508934 26.453126218885085 26.948830811801006 23.843512863804975 96-97 22.960689159035763 26.203019463915695 26.909919589505538 23.926371787543008 98-99 22.7138206331285 26.383831532819972 26.774560647077095 24.12778718697443 100-101 22.655786086878905 26.302180746220316 27.010234553577845 24.031798613322934 102-103 22.56233033748179 26.022473456614335 27.140644324635872 24.274551881268003 104-105 22.404955392364027 26.262192317715193 26.972071881220273 24.360780408700506 106-107 22.536670554033048 26.157369695711868 26.833724847941966 24.47223490231312 108-109 22.507679859642028 26.154327088708516 26.88377483787959 24.454218213769867 110-111 22.505587562746904 26.238990269232133 26.95980648189551 24.295615686125455 112-113 22.54758106360266 26.29757165084068 27.028360562212622 24.126486723344044 114-115 22.4826087447586 26.457583766930735 26.861208971687972 24.198598516622695 116-117 22.305931501365738 26.370323150364243 27.064172406616276 24.25957294165374 118-119 22.21159921355063 26.430721095448 26.856101752760566 24.501577938240803 120-121 22.194855881064374 26.577720677889015 26.946628282055517 24.28079515899109 122-123 22.19070920249094 26.568302065111666 26.903422444209646 24.33756628818775 124-125 22.100109972738093 26.49685016133757 27.02831932468779 24.37472054123655 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 11152.0 1 5819.5 2 489.0 3 452.5 4 354.5 5 286.5 6 272.0 7 265.5 8 249.5 9 244.5 10 255.0 11 261.0 12 248.5 13 274.5 14 320.0 15 368.0 16 453.5 17 563.5 18 691.0 19 864.0 20 1153.5 21 1649.5 22 2485.5 23 3601.0 24 5263.0 25 7549.0 26 11104.5 27 15555.5 28 19122.0 29 22795.5 30 27453.5 31 33778.0 32 42489.0 33 46920.5 34 48191.0 35 51624.5 36 57123.5 37 63148.0 38 67333.0 39 71310.5 40 75759.0 41 80886.0 42 84731.0 43 88382.5 44 92634.0 45 97479.5 46 100396.0 47 102949.5 48 104785.5 49 104395.0 50 104746.5 51 104065.0 52 104099.0 53 106054.0 54 107970.5 55 104099.5 56 97064.5 57 90120.0 58 84296.0 59 76119.0 60 62661.0 61 51416.0 62 42375.5 63 31718.0 64 22679.0 65 17124.0 66 12352.0 67 8878.5 68 6433.0 69 4606.0 70 3464.5 71 2418.5 72 1659.0 73 1273.5 74 978.5 75 699.5 76 462.5 77 258.0 78 149.5 79 92.0 80 61.0 81 43.0 82 39.5 83 23.5 84 14.5 85 14.5 86 16.0 87 14.5 88 9.0 89 8.5 90 9.0 91 9.0 92 8.5 93 5.0 94 4.0 95 5.5 96 4.0 97 2.0 98 3.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.46455019994723296 2 0.45666012968990594 3 0.45198321021610577 4 0.44852014770497134 5 0.4565173229884159 6 0.516853154367975 7 0.5124261466217825 8 0.5199592001253843 9 0.5251002413790272 10-11 0.5430581840914048 12-13 0.5510732102125356 14-15 0.5307054044125128 16-17 0.525225197242831 18-19 0.5349539037818427 20-21 0.5362213132575672 22-23 0.5447718645092858 24-25 0.5396129724179567 26-27 0.5462534840372455 28-29 0.5277064636812212 30-31 0.5326511457203152 32-33 0.5343112736251374 34-35 0.5328653557725503 36-37 0.48284730857564956 38-39 0.45051944152583245 40-41 0.44766330749603084 42-43 0.4600339380126091 44-45 0.46544274183154594 46-47 0.469637688687817 48-49 0.4840076130252564 50-51 0.46622817868974137 52-53 0.4656926535591536 54-55 0.45489289675896616 56-57 0.43775609258015646 58-59 0.4563031129361807 60-61 0.4523045252944585 62-63 0.46929852277177814 64-65 0.46967339036318956 66-67 0.47651026119702716 68-69 0.4860069068461176 70-71 0.48284730857564956 72-73 0.49334360113517045 74-75 0.4831864744916885 76-77 0.4809372689432197 78-79 0.49212974417250477 80-81 0.4855606359039611 82-83 0.4866138353274504 84-85 0.48423967391517786 86-87 0.47422535397318594 88-89 0.4794913510906326 90-91 0.4653534876431146 92-93 0.46451449827186037 94-95 0.45828455591935563 96-97 0.44794892089901106 98-99 0.4091590506067678 100-101 0.40594589982324103 102-103 0.39541390558834755 104-105 0.40057279767967674 106-107 0.3992339848532072 108-109 0.4123543505526084 110-111 0.43577464959698164 112-113 0.4363458764029419 114-115 0.4289734804385165 116-117 0.4226542838975804 118-119 0.4646751558110367 120-121 0.4602838497402168 122-123 0.4227792397613843 124-125 0.43256149881345485 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2800989.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.3957183435893 #Duplication Level Percentage of deduplicated Percentage of total 1 84.06826923921 60.02114472235669 2 9.68746550537325 13.832871173697312 3 2.970143593530695 6.361666063312012 4 1.2509018695774536 3.5723615022328468 5 0.6121909738327425 2.1853907170125066 6 0.35654140402760465 1.5273317795869643 7 0.23530873662138363 1.1760025398524299 8 0.16327798452749 0.9325879196026886 9 0.1085287384465421 0.6973638518082952 >10 0.5095888398695446 6.442272895832861 >50 0.029022237372694347 1.4053657993362076 >100 0.008651368644407392 0.9544134916773874 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 1.095089661283511E-4 0.8912275436918875 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 11399 0.4069633975713578 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 3.570167537252021E-5 0.0 0.0 0.0 0.0 3 3.570167537252021E-5 0.0 0.0 0.0 0.0 4 3.570167537252021E-5 0.0 0.0 0.0 0.0 5 7.140335074504041E-5 0.0 0.0 0.0 0.0 6 7.140335074504041E-5 0.0 0.0 0.0 0.0 7 1.0710502611756061E-4 0.0 0.0 0.0 0.0 8 1.0710502611756061E-4 0.0 0.0 0.0 0.0 9 1.0710502611756061E-4 0.0 0.0 3.570167537252021E-4 0.0 10-11 1.4280670149008083E-4 0.0 0.0 3.570167537252021E-4 0.0 12-13 2.1421005223512123E-4 0.0 0.0 3.570167537252021E-4 0.0 14-15 2.4991172760764147E-4 0.0 0.0 3.9271842909772227E-4 5.355251305878031E-5 16-17 2.4991172760764147E-4 0.0 0.0 4.8197261752902276E-4 1.0710502611756061E-4 18-19 3.748675914114622E-4 0.0 0.0 5.890776436465834E-4 1.0710502611756061E-4 20-21 4.641217798427627E-4 0.0 0.0 6.247793190191037E-4 1.0710502611756061E-4 22-23 4.641217798427627E-4 0.0 0.0 6.783318320778839E-4 1.0710502611756061E-4 24-25 5.355251305878031E-4 0.0 0.0 7.675860205091844E-4 1.0710502611756061E-4 26-27 5.355251305878031E-4 0.0 0.0 9.460943973717855E-4 1.0710502611756061E-4 28-29 6.783318320778839E-4 0.0 0.0 0.002070697171606172 1.0710502611756061E-4 30-31 7.140335074504042E-4 0.0 0.0 0.005819373085720794 1.0710502611756061E-4 32-33 7.140335074504042E-4 0.0 0.0 0.014548432714301982 1.0710502611756061E-4 34-35 8.389893712542249E-4 0.0 0.0 0.028757699512565026 1.0710502611756061E-4 36-37 9.282435596855254E-4 0.0 0.0 0.053338303006545185 1.0710502611756061E-4 38-39 9.639452350580455E-4 0.0 0.0 0.09128918392753416 1.0710502611756061E-4 40-41 9.817960727443056E-4 0.0 0.0 0.13298874076263778 1.0710502611756061E-4 42-43 0.001035348585803086 0.0 0.0 0.17472399927311388 1.0710502611756061E-4 44-45 0.0011067519365481264 0.0 0.0 0.2275981804998163 1.0710502611756061E-4 46-47 0.0012138569626656869 0.0 0.0 0.2955920212467811 1.6065753917634092E-4 48-49 0.0013388128264695078 0.0 0.0 0.36756659879778175 1.7850837686260104E-4 50-51 0.0013566636641557678 0.0 0.0 0.44816313095124616 1.7850837686260104E-4 52-53 0.0013566636641557678 0.0 0.0 0.5267425184461632 1.9635921454886113E-4 54-55 0.0013566636641557678 0.0 0.0 0.6164251269819339 2.1421005223512123E-4 56-57 0.0014637686902733284 0.0 0.0 0.7264933921554138 2.1421005223512123E-4 58-59 0.0014637686902733284 0.0 0.0 0.8496641721906084 2.1421005223512123E-4 60-61 0.0014637686902733284 0.0 0.0 0.9852769860931263 2.1421005223512123E-4 62-63 0.0015351720410183688 0.0 0.0 1.1253882110925821 2.1421005223512123E-4 64-65 0.0015351720410183688 0.0 0.0 1.2723006052505026 2.1421005223512123E-4 66-67 0.001570873716390889 0.0 0.0 1.4470424553613026 2.1421005223512123E-4 68-69 0.0016779787425084498 0.0 0.0 1.6437765375015752 2.1421005223512123E-4 70-71 0.0017672329309397503 0.0 0.0 1.8617352656508113 2.1421005223512123E-4 72-73 0.0018564871193710508 0.0 0.0 2.0940639181374863 2.1421005223512123E-4 74-75 0.0018564871193710508 0.0 0.0 2.3232865248667522 2.1421005223512123E-4 76-77 0.0018743379570573108 0.0 0.0 2.5784999512672133 2.1421005223512123E-4 78-79 0.001927890470116091 0.0 0.0 2.8787688919877943 2.1421005223512123E-4 80-81 0.0021778021977237327 0.0 0.0 3.211990479077212 2.1421005223512123E-4 82-83 0.0022135038730962528 0.0 0.0 3.5629557988267715 2.1421005223512123E-4 84-85 0.002249205548468773 0.0 0.0 3.9113148962741375 2.1421005223512123E-4 86-87 0.0023206088992138137 0.0 0.0 4.274543741514158 2.3206088992138135E-4 88-89 0.0023563105745863337 0.0 0.0 4.681400034059399 2.4991172760764147E-4 90-91 0.0026776256529390157 0.0 0.0 5.140202264271656 2.4991172760764147E-4 92-93 0.0026776256529390157 0.0 0.0 5.6118927992933925 2.8561340298016166E-4 94-95 0.0027133273283115357 0.0 0.0 6.087421264417675 3.3916591603894196E-4 96-97 0.0027133273283115357 0.0 0.0 6.574285011472734 3.9271842909772227E-4 98-99 0.0028561340298016167 0.0 0.0 7.092423426154119 4.2842010447024246E-4 100-101 0.0029275373805466567 0.0 0.0 7.678020156451882 4.2842010447024246E-4 102-103 0.0031060457574092577 0.0 0.0 8.296462428092363 4.641217798427627E-4 104-105 0.0035701675372520206 0.0 0.0 8.914012157848532 4.998234552152829E-4 106-107 0.003659421725683321 0.0 0.0 9.522654319599255 4.998234552152829E-4 108-109 0.0037129742387421016 0.0 0.0 10.157680019450272 4.998234552152829E-4 110-111 0.0037308250764283616 0.0 0.0 10.860056929891549 4.998234552152829E-4 112-113 0.003784377589487142 0.0 0.0 11.605972033449614 4.998234552152829E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2420 0.0 50.412224 1 GTATCAA 3825 0.0 44.03037 1 TCAACGC 4370 0.0 37.729527 4 ATCAACG 4400 0.0 37.33432 3 CAACGCA 4475 0.0 36.845577 5 GTACATG 9015 0.0 34.723007 1 AACGCAG 4815 0.0 34.243813 6 TACATGG 8875 0.0 33.596195 2 TATCAAC 5140 0.0 32.18862 2 ACATGGG 9235 0.0 31.257679 3 ACGCAGA 5740 0.0 28.208435 7 GTATACG 280 3.6379788E-12 27.630114 1 CGCAGAG 5850 0.0 27.576263 8 CATGGGG 6595 0.0 25.451733 4 GAGTACA 6380 0.0 23.599195 1 GTATAGG 890 0.0 21.397217 1 GTGGTAT 1735 0.0 20.923182 1 GCAGAGT 7560 0.0 20.551367 9 AGAGTAC 6225 0.0 19.508879 10-11 CAGAGTA 6610 0.0 19.498352 10-11 >>END_MODULE