FastQCFastQC Report
Thu 26 May 2016
SRR1364098_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364098_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2800989
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40070.14305661321768848No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37750.1347738245312638No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39750.065.815571
GTATCAA67400.051.1941031
TCAACGC85500.039.8017164
ATCAACG86250.039.5245933
CAACGCA88150.038.537695
TATCAAC90200.037.9263422
AACGCAG91200.037.5770576
ACGCAGA107950.031.6947147
CGCAGAG110150.030.7915828
TACATGG91800.030.6547742
GTACATG92750.030.5841
TGGTATC18800.029.114522
GTGGTAT20800.028.6508751
ACATGGG94500.028.5822183
GCAGAGT130200.025.5928529
GAGTACA66950.025.1015051
AGAGTAC116050.024.53456910-11
CATGGGG63350.024.4173554
GAGTACT84650.023.65310512-13
CAGAGTA122300.023.57268110-11
Produced by FastQC (version 0.11.5)