FastQCFastQC Report
Thu 26 May 2016
SRR1364097_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364097_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2431536
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN97970.4029140428107994No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22100.053.0506551
GTATCAA36250.040.058861
ATCAACG37150.038.6141783
CAACGCA37200.038.566265
TCAACGC37300.038.462074
AACGCAG40000.035.5689746
TATCAAC44400.032.3049282
TACATGG75100.032.2544172
GTACATG77550.032.001551
ACATGGG75900.030.8989013
ACGCAGA46600.029.8938127
CGCAGAG46850.028.9718768
CATGGGG49000.026.7270534
TAAGGTG15150.022.3972995
GAGTACA54750.022.1749571
GGACCGA6300.021.7330516
AGGACCG7850.020.4751725
AGAGTAC53500.019.97365610-11
CAGAGTA58300.019.80979710-11
GCAGAGT64250.019.735949
Produced by FastQC (version 0.11.5)