##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364097_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2431536 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.634153884622723 31.0 31.0 34.0 27.0 34.0 2 30.69932421317225 31.0 31.0 34.0 27.0 34.0 3 30.782847549861486 31.0 31.0 34.0 27.0 34.0 4 34.26609969994275 37.0 35.0 37.0 30.0 37.0 5 34.328193783682416 37.0 35.0 37.0 30.0 37.0 6 34.363791035789724 37.0 35.0 37.0 30.0 37.0 7 34.26321551480217 37.0 35.0 37.0 30.0 37.0 8 34.21134953379263 37.0 35.0 37.0 30.0 37.0 9 35.71170239716788 39.0 37.0 39.0 30.0 39.0 10-11 35.6157060804364 39.0 35.0 39.0 30.0 39.0 12-13 35.50923552026373 39.0 35.0 39.0 30.0 39.0 14-15 36.711284965552636 40.0 37.0 41.0 30.0 41.0 16-17 36.70588303031499 40.0 37.0 41.0 30.0 41.0 18-19 36.70484767653039 40.0 37.0 41.0 30.0 41.0 20-21 36.71600029775418 40.0 37.0 41.0 30.0 41.0 22-23 36.670813839482534 40.0 37.0 41.0 30.0 41.0 24-25 36.669587042922664 40.0 37.0 41.0 30.0 41.0 26-27 36.6183768202486 40.0 37.0 41.0 30.0 41.0 28-29 36.568395039185106 40.0 36.5 41.0 30.0 41.0 30-31 36.50294875338058 40.0 36.0 41.0 30.0 41.0 32-33 36.39035593139481 40.0 36.0 41.0 29.0 41.0 34-35 36.366618466681146 40.0 36.0 41.0 29.0 41.0 36-37 36.301225439393036 40.0 36.0 41.0 29.0 41.0 38-39 36.20938081936686 40.0 36.0 41.0 29.0 41.0 40-41 36.130774333589954 40.0 36.0 41.0 28.5 41.0 42-43 36.013406546314755 40.0 35.0 41.0 28.0 41.0 44-45 35.91855333418876 40.0 35.0 41.0 28.0 41.0 46-47 35.812152688670864 40.0 35.0 41.0 27.5 41.0 48-49 35.702529183199424 40.0 35.0 41.0 27.0 41.0 50-51 35.574834590152065 39.0 35.0 41.0 27.0 41.0 52-53 35.427453881003615 39.0 35.0 41.0 27.0 41.0 54-55 35.25627340084621 39.0 35.0 41.0 26.0 41.0 56-57 35.070943222720125 39.0 34.0 41.0 26.0 41.0 58-59 34.86428578478788 38.5 34.0 41.0 26.0 41.0 60-61 34.62534854511716 38.0 34.0 40.0 26.0 41.0 62-63 34.3700013078153 38.0 34.0 40.0 25.0 41.0 64-65 34.0955638740286 37.0 34.0 40.0 25.0 41.0 66-67 33.802870078830836 37.0 33.0 40.0 24.0 41.0 68-69 33.494379889913205 36.0 33.0 39.0 23.0 41.0 70-71 33.15560945838351 36.0 33.0 39.0 23.0 41.0 72-73 32.82394852471853 35.0 32.5 39.0 22.0 40.5 74-75 32.5680604769989 35.0 32.5 37.5 22.5 40.0 76-77 32.346347740687364 35.0 32.0 37.0 22.0 39.0 78-79 32.04220521513973 35.0 32.0 37.0 22.0 39.0 80-81 31.83075122062762 35.0 32.0 36.0 21.5 39.0 82-83 31.71383849550243 35.0 32.5 36.0 22.0 37.5 84-85 31.494496071618926 35.0 32.0 36.0 21.5 37.0 86-87 31.299450224055906 35.0 32.0 35.0 20.5 37.0 88-89 31.102111792710453 35.0 32.0 35.0 20.0 36.0 90-91 30.949490363292995 35.0 32.0 35.0 20.0 36.0 92-93 30.819490437320276 35.0 32.0 35.0 19.5 36.0 94-95 30.70643556171901 35.0 32.0 35.0 19.0 36.0 96-97 30.61691087444315 35.0 32.0 35.0 18.0 35.0 98-99 30.53385678846622 35.0 32.0 35.0 17.5 35.0 100-101 30.455313842772632 35.0 32.0 35.0 16.0 35.0 102-103 29.352177183475796 33.5 29.5 34.5 10.0 35.0 104-105 30.180922676036875 34.5 31.5 35.0 10.0 35.0 106-107 30.28132320475617 35.0 32.0 35.0 9.0 35.0 108-109 30.268762625764126 35.0 32.0 35.0 7.0 35.0 110-111 30.193488807075035 35.0 32.0 35.0 3.5 35.0 112-113 30.110528077725355 35.0 32.0 35.0 2.0 35.0 114-115 30.007287780234385 35.0 32.0 35.0 2.0 35.0 116-117 29.912454308716796 35.0 32.0 35.0 2.0 35.0 118-119 29.779932108757592 35.0 31.5 35.0 2.0 35.0 120-121 29.63343417494127 35.0 31.0 35.0 2.0 35.0 122-123 29.469957878476816 35.0 31.0 35.0 2.0 35.0 124-125 28.63819988682051 34.5 30.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6265369401325671 1101 2 0.7262635366882613 1101 3 0.7346288501823395 1101 4 0.760713707525106 1101 5 0.7594580478342934 1101 6 0.768969628650261 1101 7 0.7951248186021544 1101 8 0.8423332028245198 1101 9 0.8222570059021095 1101 10-11 0.8544785563668071 1101 12-13 0.9733369194435113 1101 14-15 1.128980483274681 1101 16-17 1.1356734371307269 1101 18-19 1.0476636424824974 1101 20-21 1.073087499881325 1101 22-23 1.0448954154873746 1101 24-25 1.037518745295543 1101 26-27 1.0267461337159105 1101 28-29 1.097914732225334 1101 30-31 1.1241242015864827 1101 32-33 1.152394897430888 1101 34-35 1.1353482530056027 1101 36-37 1.0864914248509407 1101 38-39 1.153214172172163 1101 40-41 1.224142443561469 1101 42-43 1.1905505619035495 1101 44-45 1.2077801159666635 1101 46-47 1.223082029356668 1101 48-49 1.097470451540957 1101 50-51 1.1264076332843302 1101 52-53 1.1278194050582684 1101 54-55 1.163361278958888 1101 56-57 1.1747658841141018 1101 58-59 1.1763230696554317 1101 60-61 1.192857381902897 1101 62-63 1.2433132736329142 1101 64-65 1.1617709113904695 1101 66-67 1.1695973439900484 1101 68-69 1.1329592561570436 1101 70-71 1.2039142463377033 1101 72-73 1.2086661974661936 1101 74-75 1.2590322297906837 1101 76-77 1.1956747316047753 1101 78-79 1.1254125875583654 1101 80-81 1.146723658191842 1101 82-83 1.1247484221000583 1101 84-85 1.1580177040748936 1101 86-87 1.1650928212035012 1101 88-89 1.1391374433709416 1101 90-91 1.1540066340246256 1101 92-93 1.1729813293396987 1101 94-95 1.23140695586385 1101 96-97 1.1812442474654787 1101 98-99 1.1827386887874383 1101 100-101 1.2150909699138737 1101 102-103 1.3002421276343306 1101 104-105 1.2707312443856367 1101 106-107 1.2561826611935665 1101 108-109 1.3010415062812335 1101 110-111 1.2526505605416318 1101 112-113 1.3027623224529492 1101 114-115 1.3616892162238763 1101 116-117 1.3892689981475712 1101 118-119 1.3714465549789985 1101 120-121 1.446341365086596 1101 122-123 1.4077338331271712 1101 124-125 1.4338768793401435 1102 1 0.3471961133362811 1102 2 0.42572841795641736 1102 3 0.4037402545644362 1102 4 0.3930835654762461 1102 5 0.3640753917979751 1102 6 0.44081958100896657 1102 7 0.4922898625949088 1102 8 0.44394674659831423 1102 9 0.5535188115951186 1102 10-11 0.4823668406597861 1102 12-13 0.4631909695303946 1102 14-15 0.5557774843701537 1102 16-17 0.45830713908796383 1102 18-19 0.43514725501647433 1102 20-21 0.5018959441725244 1102 22-23 0.5168566879608321 1102 24-25 0.4787850400885887 1102 26-27 0.5541826099960616 1102 28-29 0.6042786399735505 1102 30-31 0.6179379727273542 1102 32-33 0.5504949290979653 1102 34-35 0.7073848385378696 1102 36-37 0.6012298746328852 1102 38-39 0.6436223609321416 1102 40-41 0.6884039809776468 1102 42-43 0.6303730574577102 1102 44-45 0.6948450084329565 1102 46-47 0.6982818970048896 1102 48-49 0.5641551674566045 1102 50-51 0.5720511171467138 1102 52-53 0.5907421960545776 1102 54-55 0.5125331448080885 1102 56-57 0.5341096553435989 1102 58-59 0.5914326896519171 1102 60-61 0.6618598366288992 1102 62-63 0.6354615197256379 1102 64-65 0.6347622431085185 1102 66-67 0.7259494416490071 1102 68-69 0.6755362457702105 1102 70-71 0.7100007682192881 1102 72-73 0.6727471368824851 1102 74-75 0.6515706871412092 1102 76-77 0.6883013390676567 1102 78-79 0.6821155060642425 1102 80-81 0.6412110053588016 1102 82-83 0.5325793272255801 1102 84-85 0.6036055489819034 1102 86-87 0.5993141058401577 1102 88-89 0.6020035051415817 1102 90-91 0.6059991135511069 1102 92-93 0.587885099269311 1102 94-95 0.5337669057198582 1102 96-97 0.5771864583563939 1102 98-99 0.6046820526919525 1102 100-101 0.6587045814954422 1102 102-103 0.5544100551458442 1102 104-105 0.5419135433478814 1102 106-107 0.5749158332231694 1102 108-109 0.5259907876102226 1102 110-111 0.578243220926371 1102 112-113 0.5891430245842102 1102 114-115 0.7293396161983274 1102 116-117 0.6158540366284839 1102 118-119 0.5502325256339837 1102 120-121 0.6245912744534792 1102 122-123 0.6215793093283999 1102 124-125 0.6572380588803703 1103 1 0.2637517519406174 1103 2 0.37622492903596694 1103 3 0.3217837982050362 1103 4 0.266453907466385 1103 5 0.37390562014586237 1103 6 0.3339687120056283 1103 7 0.3685315586755564 1103 8 0.33371444639600156 1103 9 0.4515531773342829 1103 10-11 0.4321392374901549 1103 12-13 0.41660883132201576 1103 14-15 0.5216570563851306 1103 16-17 0.46700226282750634 1103 18-19 0.4090289710985573 1103 20-21 0.5031934506380864 1103 22-23 0.519810881641888 1103 24-25 0.5723510327352557 1103 26-27 0.6072498748370876 1103 28-29 0.5906685415206496 1103 30-31 0.5453788939091808 1103 32-33 0.2960320681852906 1103 34-35 0.5550097793629973 1103 36-37 0.5209860989159125 1103 38-39 0.6350471733001228 1103 40-41 0.7028164659359533 1103 42-43 0.7196595624351616 1103 44-45 0.6872531225568963 1103 46-47 0.742948788063643 1103 48-49 0.6248056716171035 1103 50-51 0.6416844871093659 1103 52-53 0.5775025049291003 1103 54-55 0.6119183049043144 1103 56-57 0.6103400690625023 1103 58-59 0.6550392702114252 1103 60-61 0.6787981620656254 1103 62-63 0.6751741081846134 1103 64-65 0.6086347547916517 1103 66-67 0.6362495406172144 1103 68-69 0.6417104980121593 1103 70-71 0.689481373748805 1103 72-73 0.6677128536342565 1103 74-75 0.6419228383800828 1103 76-77 0.7195628249546999 1103 78-79 0.7665512230649583 1103 80-81 0.6649150870007112 1103 82-83 0.5914313127504052 1103 84-85 0.5968532855888213 1103 86-87 0.5362143599303764 1103 88-89 0.5777714257053859 1103 90-91 0.546155040253133 1103 92-93 0.48773600892541324 1103 94-95 0.6004388997810679 1103 96-97 0.552929031957941 1103 98-99 0.5812451426756198 1103 100-101 0.605122428155596 1103 102-103 0.5747413870105156 1103 104-105 0.5720580196381526 1103 106-107 0.5597573038378805 1103 108-109 0.5761111107208841 1103 110-111 0.5278860588684928 1103 112-113 0.6221262211868961 1103 114-115 0.6410434557366465 1103 116-117 0.6092204890627677 1103 118-119 0.6485277663530731 1103 120-121 0.6602359020933548 1103 122-123 0.6608073071017699 1103 124-125 0.6830512727573392 1104 1 0.35916372153758047 1104 2 0.32706780442624606 1104 3 0.36813640636268374 1104 4 0.3377533237906789 1104 5 0.3532949285013629 1104 6 0.2919141058054464 1104 7 0.3544785673220616 1104 8 0.35916169742086623 1104 9 0.37620515489540196 1104 10-11 0.39024046069248897 1104 12-13 0.3873970446474502 1104 14-15 0.5096491453425998 1104 16-17 0.47214762586048664 1104 18-19 0.5546368591897917 1104 20-21 0.4839180759354562 1104 22-23 0.5445909557877897 1104 24-25 0.5700336809627231 1104 26-27 0.6210722180352164 1104 28-29 0.6851030514106924 1104 30-31 0.6517884633893445 1104 32-33 -0.14079465763738597 1104 34-35 0.41870827774546626 1104 36-37 0.5767118628343013 1104 38-39 0.6503638963887255 1104 40-41 0.7130326140450265 1104 42-43 0.7119642043075487 1104 44-45 0.6386701931760328 1104 46-47 0.6733204614842947 1104 48-49 0.6633217464003565 1104 50-51 0.716693640796791 1104 52-53 0.66446810811685 1104 54-55 0.7590157569663063 1104 56-57 0.7342580866978494 1104 58-59 0.6984891515540426 1104 60-61 0.7403871706507559 1104 62-63 0.7793161362619827 1104 64-65 0.6820557557516693 1104 66-67 0.6884274164746316 1104 68-69 0.6690832225416941 1104 70-71 0.6665226116438205 1104 72-73 0.5785686315614598 1104 74-75 0.666894826926999 1104 76-77 0.6438385071881996 1104 78-79 0.5869333786788857 1104 80-81 0.5484190552497701 1104 82-83 0.4896119683566944 1104 84-85 0.5577111068419711 1104 86-87 0.5205964698618644 1104 88-89 0.4960885768604584 1104 90-91 0.5501246652842475 1104 92-93 0.49287693443235625 1104 94-95 0.5723462296521689 1104 96-97 0.50466976678198 1104 98-99 0.4945036861516101 1104 100-101 0.533879082896668 1104 102-103 0.5672732714950435 1104 104-105 0.5470193650024804 1104 106-107 0.5259429770821278 1104 108-109 0.48165613317201306 1104 110-111 0.5834770114340238 1104 112-113 0.5973994317528053 1104 114-115 0.5719220047082878 1104 116-117 0.5442298243791548 1104 118-119 0.6562285251906665 1104 120-121 0.5936860489995439 1104 122-123 0.6700267318250219 1104 124-125 0.7894232092648004 1105 1 0.24996651430139138 1105 2 0.22865866138749524 1105 3 0.3138535059544445 1105 4 0.26788014058564613 1105 5 0.24294818444194277 1105 6 0.309880232250201 1105 7 0.259621982945589 1105 8 0.28529318591201047 1105 9 0.3424958096106394 1105 10-11 0.3483324953082416 1105 12-13 0.28772341110314414 1105 14-15 0.3875356202265223 1105 16-17 0.3939330531694196 1105 18-19 0.4668485505899582 1105 20-21 0.4480385688686539 1105 22-23 0.3883756457289138 1105 24-25 0.45423968923536506 1105 26-27 0.44700660760029365 1105 28-29 0.4353659081378751 1105 30-31 0.4546814509731405 1105 32-33 0.10201520091925431 1105 34-35 0.4455062090355142 1105 36-37 0.4431567554616578 1105 38-39 0.4384736256845514 1105 40-41 0.5414022701919166 1105 42-43 0.49604219994481014 1105 44-45 0.4650812747915367 1105 46-47 0.46304215949177774 1105 48-49 0.5021894989330633 1105 50-51 0.5310109977828006 1105 52-53 0.4763758332593113 1105 54-55 0.4384930821008197 1105 56-57 0.4380582151936565 1105 58-59 0.5140078516929734 1105 60-61 0.4673546602998897 1105 62-63 0.5111037955379274 1105 64-65 0.5608475663788681 1105 66-67 0.611074325334549 1105 68-69 0.5981512306970203 1105 70-71 0.5731832078434138 1105 72-73 0.4653777401342438 1105 74-75 0.4957982493256168 1105 76-77 0.4901569427122041 1105 78-79 0.4741777745023299 1105 80-81 0.4900249669156729 1105 82-83 0.4579875594152476 1105 84-85 0.4967390496461732 1105 86-87 0.48825838175433134 1105 88-89 0.48943491948792683 1105 90-91 0.38060589911238196 1105 92-93 0.4434296046258801 1105 94-95 0.444934970472481 1105 96-97 0.46420073562610753 1105 98-99 0.4745865682263144 1105 100-101 0.5202068422178812 1105 102-103 0.44845686359830594 1105 104-105 0.5312766159089897 1105 106-107 0.5821899883199428 1105 108-109 0.5302048378996886 1105 110-111 0.4845427253473922 1105 112-113 0.5123374648060448 1105 114-115 0.5518126262460079 1105 116-117 0.5813771437304176 1105 118-119 0.6073716331973351 1105 120-121 0.5859484179956311 1105 122-123 0.6746818862125856 1105 124-125 0.7504538102974116 1106 1 0.09185272897776642 1106 2 0.10781065352949426 1106 3 0.16663345865753598 1106 4 0.23242935571133927 1106 5 0.18515688529784313 1106 6 0.15341063073832828 1106 7 0.11816464164197527 1106 8 0.0970708693268918 1106 9 0.1728064446868416 1106 10-11 0.12501544455076186 1106 12-13 0.24819620900868244 1106 14-15 0.2991712417007264 1106 16-17 0.2338744765082339 1106 18-19 0.2865870578765026 1106 20-21 0.2591435682903338 1106 22-23 0.2524509759841109 1106 24-25 0.2968978282040382 1106 26-27 0.28675542313696667 1106 28-29 0.3546899154711056 1106 30-31 0.360939482519413 1106 32-33 0.24906025880213178 1106 34-35 0.38184330832418567 1106 36-37 0.38529567953755617 1106 38-39 0.40964347271911095 1106 40-41 0.4534883052602723 1106 42-43 0.36555753102816624 1106 44-45 0.3299990028914337 1106 46-47 0.3973974389661947 1106 48-49 0.37411546348008073 1106 50-51 0.30682746076298173 1106 52-53 0.3339568464898264 1106 54-55 0.33684533479588197 1106 56-57 0.38999973170088964 1106 58-59 0.37194372115843066 1106 60-61 0.3465109326152742 1106 62-63 0.29265827886028006 1106 64-65 0.3539869859594873 1106 66-67 0.4133825730693985 1106 68-69 0.37926057598777163 1106 70-71 0.39669443911789415 1106 72-73 0.34661039423392737 1106 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scores pass #Quality Count 2 58840.0 3 10091.0 4 5592.0 5 4654.0 6 4835.0 7 5574.0 8 6641.0 9 7525.0 10 8359.0 11 8914.0 12 9810.0 13 10082.0 14 9791.0 15 9007.0 16 8152.0 17 7455.0 18 7252.0 19 7207.0 20 7586.0 21 8132.0 22 9638.0 23 12429.0 24 16382.0 25 20589.0 26 25364.0 27 30133.0 28 35973.0 29 43668.0 30 51348.0 31 61907.0 32 78608.0 33 101613.0 34 136697.0 35 200139.0 36 363812.0 37 685299.0 38 350318.0 39 2120.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.93405464678655 18.782906465997907 12.594476651102527 24.68856223611302 2 15.429329475188464 19.774576152847317 40.23024928248503 24.56584508947919 3 18.59141139776506 24.1998636729804 29.960630409187615 27.248094520066925 4 11.95704216013639 15.893260582996897 38.493881312794365 33.65581594407235 5 12.91520415650346 38.337472980744444 35.69950125924604 13.047821603506057 6 32.5631105766667 37.13608256201514 17.323770103843245 12.977036757474908 7 28.193601883588542 32.407412000271165 21.77258545344517 17.626400662695126 8 25.87671722149098 35.980461541514266 20.283387018896878 17.859434218097878 9 26.081172256637018 15.340144266545838 20.18429373224367 38.39438974457347 10-11 24.57292505710321 26.28281677188653 28.934435922839135 20.209822248171125 12-13 24.9566262892966 23.732589262176532 28.706604320726647 22.604180127800216 14-15 21.87782107167804 25.72310908223064 25.841414505365297 26.55765534072603 16-17 22.232796380165322 28.827109072531055 26.816025499246553 22.12406904805707 18-19 22.233093804782854 27.916225647471304 28.0429084113386 21.807772136407237 20-21 22.686638131081082 27.198058394930165 28.045567354077278 22.06973611991148 22-23 22.347373248146376 27.34547734070139 27.812197238880614 22.49495217227162 24-25 22.219764348738067 27.446696476766995 27.91944202504702 22.41409714944792 26-27 21.948333025959194 27.68056438854186 27.69952372170263 22.671578863796316 28-29 22.14052529082741 28.051333877535495 27.585427095686494 22.222713735950602 30-31 22.235842954382285 27.570571528448045 27.539728494477504 22.65385702269216 32-33 21.81501247814877 27.77661550267342 27.54001442139924 22.868357597778573 34-35 22.566553341279754 27.833682487982177 27.403203126970325 22.196561043767744 36-37 22.705062578449045 27.32337794490617 27.345919616522984 22.625639860121797 38-39 22.246971419152782 27.688802073920275 27.404259748972365 22.659966757954574 40-41 22.56292747369321 27.493480361357143 27.549247902417545 22.394344262532105 42-43 22.28653939110999 27.557705734836624 27.411742347560597 22.74401252649279 44-45 22.263919571995963 27.77867755544547 27.645700974775973 22.311701897782594 46-47 22.611014484609804 27.90117254004066 27.0502097193337 22.437603256015834 48-49 22.26039589088052 27.85957636456689 27.207120688597147 22.67290705595544 50-51 22.416863332275636 27.89871060077212 27.2885101010101 22.395915965942145 52-53 22.67180090503346 27.607197711903535 27.33296079806557 22.388040584997434 54-55 22.03494931609677 27.88095964993673 27.507125326005095 22.576965707961403 56-57 22.233473435376304 28.097746629609947 27.264321563205872 22.40445837180787 58-59 22.226277749203184 27.614214036805457 27.373423147075165 22.786085066916193 60-61 21.979970193863817 27.890776932157475 27.58220183277787 22.547051041200838 62-63 22.073206254635302 27.90421375994976 27.728015502306548 22.294564483108395 64-65 21.89382203050012 27.889127129836545 27.506692908749812 22.71035793091352 66-67 21.830083994202738 28.016420758168803 27.440826492211606 22.712668755416857 68-69 22.320445641932658 27.430637056382963 27.721044018711673 22.52787328297271 70-71 22.090042006710412 27.546720094315198 27.404962167927255 22.958275731047134 72-73 22.022132187676224 27.639198186047505 27.472193218259296 22.866476408016982 74-75 21.90995170966625 27.935127478051164 27.494621780816598 22.660299031465993 76-77 22.05340453442055 27.643341396003844 27.58028355779265 22.722970511782954 78-79 22.201248816500676 27.665864511385767 27.485059289648312 22.64782738246524 80-81 22.297432299050143 27.710512281867533 27.338160415242275 22.653895003840052 82-83 22.09198941506607 27.76049138491591 27.624284848531182 22.523234351486835 84-85 22.065135395529246 27.8197682286894 27.475894432418148 22.6392019433632 86-87 22.1737633942978 27.799358139753977 27.36705076084033 22.659827705107897 88-89 22.031053077415756 27.86886634573666 27.290923741245017 22.809156835602572 90-91 21.96712851946541 27.81759678994677 27.448669556551902 22.76660513403592 92-93 22.095989095969884 27.467438608538007 27.762577056296106 22.673995239196003 94-95 21.858823033645198 27.31407307764448 27.914632302331672 22.91247158637865 96-97 22.067324116695243 27.066276927932346 27.890292622131746 22.97610633324066 98-99 21.76820493338261 27.337513652796737 27.65258613392026 23.241695279900398 100-101 21.739672626796157 27.12652083849923 27.9865666363096 23.14723989839501 102-103 21.57338693095535 26.823411910331885 28.14389194375883 23.459309214953937 104-105 21.4775564572437 27.202165923372657 27.790057050791596 23.530220568592046 106-107 21.669331919572535 27.075728278808164 27.57923604891844 23.675703752700866 108-109 21.611935121206397 27.001673485526567 27.66931265272749 23.71707874053954 110-111 21.648905631074378 27.104434889282658 27.809733873599253 23.436925606043708 112-113 21.621302906746088 27.221085314351413 27.92388231423638 23.233729464666123 114-115 21.58274852104426 27.382720725122496 27.69409287100689 23.340437882826354 116-117 21.40667118477192 27.2110942198401 27.907993370692363 23.474241224695618 118-119 21.269372428448985 27.383458655936003 27.708886325902395 23.63828258971262 120-121 21.328777791319546 27.532756729511938 27.759611153916865 23.378854325251652 122-123 21.307392154044646 27.487729853584238 27.702178642759083 23.50269934961203 124-125 21.238127513974586 27.405635020402986 27.771730229939678 23.58450723568275 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 9576.0 1 5025.5 2 434.0 3 363.0 4 300.0 5 252.5 6 216.5 7 198.0 8 202.0 9 211.0 10 215.0 11 210.0 12 229.0 13 259.0 14 284.0 15 339.5 16 412.0 17 501.0 18 648.5 19 882.5 20 1244.0 21 1872.5 22 2788.5 23 4139.0 24 6010.0 25 8620.5 26 12511.5 27 17099.0 28 21373.5 29 26209.5 30 31994.0 31 38890.0 32 48740.5 33 53749.5 34 55112.0 35 59793.0 36 65207.0 37 69991.0 38 72457.5 39 74744.5 40 78344.5 41 82190.5 42 83347.0 43 84234.0 44 86359.0 45 90057.0 46 90942.5 47 90109.5 48 89630.5 49 86759.5 50 85097.5 51 82904.5 52 80592.0 53 79092.5 54 77987.0 55 73615.5 56 67111.0 57 60876.0 58 56073.5 59 49902.0 60 39917.0 61 32631.5 62 27288.5 63 19712.0 64 13301.5 65 9794.5 66 6969.5 67 5169.5 68 3847.5 69 2786.5 70 2008.5 71 1286.5 72 849.0 73 645.5 74 495.0 75 372.0 76 250.5 77 141.5 78 86.5 79 54.0 80 35.5 81 29.5 82 17.0 83 9.0 84 12.0 85 8.5 86 6.5 87 8.0 88 9.0 89 8.5 90 6.0 91 7.5 92 7.5 93 4.5 94 3.0 95 3.0 96 3.0 97 2.0 98 2.0 99 2.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.46180685788736014 2 0.4535816043850472 3 0.4477005481308934 4 0.44568536102282674 5 0.4540339933276743 6 0.5103358535510065 7 0.5068401208125234 8 0.5145718591046976 9 0.5214810720466405 10-11 0.5390831145415902 12-13 0.5473289311776589 14-15 0.5262722822117377 16-17 0.5198771476136894 18-19 0.5303643458291384 20-21 0.5312896868481487 22-23 0.5415918168597956 24-25 0.5348265458541432 26-27 0.5429489836876773 28-29 0.5224886656006739 30-31 0.527958459179712 32-33 0.5308578610392772 34-35 0.5279378960459561 36-37 0.4759748570450941 38-39 0.44443100986372397 40-41 0.4430532799020866 42-43 0.4551032762829751 44-45 0.45999730211685125 46-47 0.4638014818616709 48-49 0.4771263925354179 50-51 0.4599561758493397 52-53 0.45993561271558386 54-55 0.4501269979140757 56-57 0.4343962005909022 58-59 0.4511962808693764 60-61 0.4480089951372302 62-63 0.46271163577261454 64-65 0.4626910726388587 66-67 0.4700115482559173 68-69 0.4793677741147982 70-71 0.47564584690500156 72-73 0.4872434543432629 74-75 0.4766534404590349 76-77 0.47441205887965465 78-79 0.4854955879740213 80-81 0.4790182008409499 82-83 0.4804164939363431 84-85 0.4773731501404873 86-87 0.46814030308414106 88-89 0.4723763086378322 90-91 0.4581466200788308 92-93 0.458413940817656 94-95 0.45411624586269744 96-97 0.4428887748320403 98-99 0.4059573866066552 100-101 0.4032224898171362 102-103 0.39226233952530415 104-105 0.3972386178942035 106-107 0.39592257733383346 108-109 0.4089390410012436 110-111 0.4320725664764988 112-113 0.43164074066762737 114-115 0.4243202650505689 116-117 0.4181307617900784 118-119 0.4598944864480723 120-121 0.45407511959518587 122-123 0.41998144382809877 124-125 0.42954330102453764 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2431536.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.33938136164946 #Duplication Level Percentage of deduplicated Percentage of total 1 84.3411076602578 61.01183551499324 2 9.70608571149851 14.042644716258948 3 2.8930183291715506 6.278374686005482 4 1.1974309067272195 3.464856440638641 5 0.598244633522716 2.1638323345979984 6 0.3378775015465686 1.4665109662739118 7 0.20802777257483734 1.0534020261873949 8 0.1358141477032708 0.7859769140011436 9 0.09648940926677975 0.6281985756878868 >10 0.4486076350669551 5.799194674553351 >50 0.027364793932794236 1.3813918553155098 >100 0.009813267509222129 1.0858660820947295 >500 0.0 0.0 >1k 0.0 0.0 >5k 1.1823122168935627E-4 0.8379152133916872 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 9797 0.4029140428107994 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.225253502312941E-5 2 0.0 0.0 0.0 0.0 8.225253502312941E-5 3 0.0 0.0 0.0 0.0 8.225253502312941E-5 4 0.0 0.0 0.0 0.0 8.225253502312941E-5 5 0.0 0.0 0.0 0.0 8.225253502312941E-5 6 0.0 0.0 0.0 0.0 8.225253502312941E-5 7 0.0 0.0 0.0 0.0 8.225253502312941E-5 8 0.0 0.0 0.0 0.0 8.225253502312941E-5 9 0.0 0.0 0.0 0.0 8.225253502312941E-5 10-11 0.0 0.0 0.0 4.1126267511564704E-5 8.225253502312941E-5 12-13 0.0 0.0 0.0 4.1126267511564704E-5 4.523889426272118E-4 14-15 0.0 0.0 0.0 4.1126267511564704E-5 5.552046114061235E-4 16-17 0.0 0.0 0.0 4.1126267511564704E-5 6.168940126734706E-4 18-19 0.0 0.0 0.0 8.225253502312941E-5 6.991465476966E-4 20-21 0.0 0.0 0.0 8.225253502312941E-5 7.813990827197294E-4 22-23 0.0 0.0 0.0 1.0281566877891178E-4 9.047778852544235E-4 24-25 0.0 0.0 0.0 2.0563133755782353E-4 9.047778852544235E-4 26-27 0.0 0.0 0.0 2.4675760506938827E-4 9.253410190102058E-4 28-29 0.0 0.0 0.0 0.0013777299616374175 9.459041527659882E-4 30-31 0.0 0.0 0.0 0.0049351521013877644 9.664672865217707E-4 32-33 0.0 0.0 0.0 0.01371561021510683 0.0010692829553006823 34-35 0.0 0.0 0.0 0.028274308914200737 0.0010692829553006823 36-37 0.0 0.0 0.0 0.05403991551019603 0.0010692829553006823 38-39 0.0 0.0 0.0 0.0940352106651927 0.0011309723565680295 40-41 0.0 0.0 0.0 0.13919185239289075 0.0011515354903238117 42-43 0.0 0.0 0.0 0.1859935448210514 0.0011515354903238117 44-45 0.0 0.0 0.0 0.24192526863677938 0.001172098624079594 46-47 0.0 0.0 0.0 0.3131970902343211 0.0012132248915911587 48-49 0.0 0.0 0.0 0.39606651927012393 0.0012337880253469411 50-51 0.0 0.0 0.0 0.49086256588428057 0.001274914292858506 52-53 0.0 0.0 0.0 0.5804972659257359 0.0013982930953932 54-55 0.0 0.0 0.0 0.6768149844378204 0.0014599824966605472 56-57 0.0 0.0 0.0 0.792873311355456 0.0015011087641721118 58-59 0.0 0.0 0.0 0.9215985286666535 0.0015833612991952412 60-61 0.0 0.0 0.0 1.0686249350204973 0.0016039244329510236 62-63 0.0 0.0 0.0 1.2159597883806779 0.001809555770508847 64-65 0.0 0.0 0.0 1.3641171670910897 0.001809555770508847 66-67 0.0 0.0 0.0 1.53773170539116 0.001871245171776194 68-69 0.0 0.0 0.0 1.7509097130373559 0.0018918083055319765 70-71 0.0 0.0 0.0 1.9774331944910544 0.001912371439287759 72-73 0.0 0.0 0.0 2.221826039178527 0.002035750241822453 74-75 0.0 0.0 0.0 2.457479552019793 0.0021796921781129294 76-77 0.0 0.0 0.0 2.7180555829730673 0.002220818445624494 78-79 0.0 0.0 0.0 3.0225544676286926 0.002220818445624494 80-81 0.0 0.0 0.0 3.3604273183699522 0.002220818445624494 82-83 0.0 0.0 0.0 3.7203849747649222 0.0024058866494265354 84-85 0.0 0.0 0.0 4.0776488606378845 0.002426449783182318 86-87 0.0 0.0 0.0 4.451774516190589 0.002488139184449665 88-89 0.0 0.0 0.0 4.863880279790223 0.0025909548532285767 90-91 0.0 0.0 0.0 5.3271882464417555 0.002632081120740141 92-93 0.0 0.0 0.0 5.801620868455165 0.0028377124582979647 94-95 0.0 6.168940126734705E-5 0.0 6.2811325845062544 0.0028377124582979647 96-97 0.0 8.225253502312941E-5 0.0 6.776333971613005 0.0031050331971231356 98-99 0.0 8.225253502312941E-5 0.0 7.298637569009877 0.0033312276684367413 100-101 0.0 8.225253502312941E-5 0.0 7.888491060794493 0.0033312276684367413 102-103 0.0 8.225253502312941E-5 0.0 8.512684985951267 0.0034340433372156533 104-105 0.0 8.225253502312941E-5 0.0 9.134596403261149 0.003536859005994565 106-107 0.0 8.225253502312941E-5 0.0 9.745033591935304 0.003536859005994565 108-109 0.0 8.225253502312941E-5 0.0 10.383395516249811 0.003536859005994565 110-111 0.0 8.225253502312941E-5 0.0 11.079272525679242 0.0035779852735061293 112-113 0.0 8.225253502312941E-5 0.0 11.819627593422428 0.0035779852735061293 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2210 0.0 53.050655 1 GTATCAA 3625 0.0 40.05886 1 ATCAACG 3715 0.0 38.614178 3 CAACGCA 3720 0.0 38.56626 5 TCAACGC 3730 0.0 38.46207 4 AACGCAG 4000 0.0 35.568974 6 TATCAAC 4440 0.0 32.304928 2 TACATGG 7510 0.0 32.254417 2 GTACATG 7755 0.0 32.00155 1 ACATGGG 7590 0.0 30.898901 3 ACGCAGA 4660 0.0 29.893812 7 CGCAGAG 4685 0.0 28.971876 8 CATGGGG 4900 0.0 26.727053 4 TAAGGTG 1515 0.0 22.397299 5 GAGTACA 5475 0.0 22.174957 1 GGACCGA 630 0.0 21.733051 6 AGGACCG 785 0.0 20.475172 5 AGAGTAC 5350 0.0 19.973656 10-11 CAGAGTA 5830 0.0 19.809797 10-11 GCAGAGT 6425 0.0 19.73594 9 >>END_MODULE