FastQCFastQC Report
Thu 26 May 2016
SRR1364097_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364097_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2431536
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36480.15002862388218804No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35790.1471909114238901No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34150.075.7572251
GTATCAA63100.049.5024831
ATCAACG77400.040.0480653
TCAACGC77700.039.970834
CAACGCA78150.039.7406735
AACGCAG82300.038.025916
TATCAAC82600.037.671712
ACGCAGA96150.032.3654637
CGCAGAG96400.032.158088
GTACATG74500.029.6853871
TACATGG74400.028.9487192
ACATGGG75850.026.9044023
GCAGAGT116900.026.3660269
GAGTACT78600.025.66242412-13
CAGAGTA109350.025.22062910-11
TGGTATC16500.024.8804862
AGAGTAC105900.024.1600310-11
GTGGTAT17700.023.57491
GTACTTT87450.023.06346914-15
TAAGGTG16000.022.3113045
Produced by FastQC (version 0.11.5)