FastQCFastQC Report
Thu 26 May 2016
SRR1364096_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364096_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1913982
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN75810.39608522964165804No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19300.057.3502541
GTATCAA31650.044.9369771
GTACATG77000.038.719231
TCAACGC34950.038.1520044
ATCAACG35350.037.7153433
CAACGCA36000.037.0421565
TACATGG80300.036.3917272
AACGCAG37000.036.2019126
ACATGGG79450.035.0599633
TATCAAC39950.034.4137422
ACGCAGA46350.028.7721147
CGCAGAG47200.028.2539738
CATGGGA54950.028.1658024
GAGTACA56850.025.8550951
CATGGGG33400.025.4862584
ATGGGAG27900.024.3249155
AGTACAT57850.023.4567682
GCAGAGT55150.023.3174869
TGGACCG2701.1004522E-619.844015
CAGAGTA55400.019.71993610-11
Produced by FastQC (version 0.11.5)