FastQCFastQC Report
Thu 26 May 2016
SRR1364096_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364096_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1913982
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49160.2568467206065679No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47100.24608381897008436No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26110.13641716588766248No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35450.075.981991
GTATCAA62700.048.0918461
ATCAACG79550.037.394093
TCAACGC80150.037.1151244
CAACGCA81950.036.2999085
TATCAAC83050.035.6032682
AACGCAG84150.035.5629966
TGGTATC14400.033.052222
GTACATG72900.032.534491
TACATGG76450.030.5835692
ACGCAGA98250.030.4640717
CGCAGAG98100.030.1467118
ACATGGG76150.029.454023
GTGGTAT16100.029.2408281
GCAGAGT109300.027.0031269
GAGTACT75200.026.19163912-13
CAGAGTA107600.025.49418810-11
GAGTACA56150.024.8344521
AGAGTAC104250.024.17296410-11
CATGGGG29950.024.0365984
Produced by FastQC (version 0.11.5)