##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364096_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1913982 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.05738246232201 33.0 31.0 34.0 30.0 34.0 2 32.08311415676845 33.0 31.0 34.0 30.0 34.0 3 32.22029726507355 34.0 31.0 34.0 30.0 34.0 4 35.86892980184766 37.0 35.0 37.0 35.0 37.0 5 35.80546421021723 37.0 35.0 37.0 35.0 37.0 6 35.83517713332727 37.0 35.0 37.0 35.0 37.0 7 35.74913034709835 37.0 35.0 37.0 33.0 37.0 8 35.727338606110195 37.0 35.0 37.0 33.0 37.0 9 37.374490460202864 39.0 37.0 39.0 34.0 39.0 10-11 37.298032322143044 39.0 37.0 39.0 33.0 39.0 12-13 37.246987171248215 39.0 37.0 39.0 33.0 39.0 14-15 38.55222671895556 40.0 38.0 41.0 34.0 41.0 16-17 38.52941250231193 40.0 38.0 41.0 33.5 41.0 18-19 38.66607366213475 40.0 38.0 41.0 34.0 41.0 20-21 38.7233249842475 40.0 38.0 41.0 34.0 41.0 22-23 38.69655879731366 40.0 38.0 41.0 34.0 41.0 24-25 38.67344755593313 40.0 38.0 41.0 34.0 41.0 26-27 38.56716651462762 40.0 38.0 41.0 34.0 41.0 28-29 38.49157541711469 40.0 38.0 41.0 34.0 41.0 30-31 38.37071900362699 40.0 38.0 41.0 34.0 41.0 32-33 38.24877219325991 40.0 38.0 41.0 33.5 41.0 34-35 38.137932331652024 40.0 37.5 41.0 33.0 41.0 36-37 38.03362936537543 40.0 37.0 41.0 33.0 41.0 38-39 37.93949577373246 40.0 37.0 41.0 33.0 41.0 40-41 37.820423598550036 40.0 37.0 41.0 33.0 41.0 42-43 37.69583543627892 40.0 37.0 41.0 33.0 41.0 44-45 37.539143785051266 40.0 37.0 41.0 32.5 41.0 46-47 37.302205820117436 40.0 37.0 41.0 31.5 41.0 48-49 37.075425474220765 40.0 37.0 41.0 31.0 41.0 50-51 36.924892188118804 40.0 36.0 41.0 31.0 41.0 52-53 36.78954739386264 40.0 36.0 41.0 31.0 41.0 54-55 36.64049322302927 40.0 36.0 41.0 30.0 41.0 56-57 36.46134315787714 39.0 35.5 41.0 30.0 41.0 58-59 36.27767528639245 39.0 35.0 41.0 30.0 41.0 60-61 36.06448127516351 39.0 35.0 41.0 29.0 41.0 62-63 35.83062197032156 38.5 35.0 40.5 29.0 41.0 64-65 35.639406744682034 38.0 35.0 40.0 29.0 41.0 66-67 35.447809592775684 37.5 35.0 40.0 29.0 41.0 68-69 35.14970490840562 37.0 34.5 40.0 29.0 41.0 70-71 34.83775500501049 36.5 34.0 39.0 29.0 41.0 72-73 34.50432423084439 36.0 34.0 39.0 29.0 41.0 74-75 34.223829691188314 36.0 34.0 39.0 28.5 40.0 76-77 34.005854025795436 35.0 34.0 37.5 29.0 39.5 78-79 33.699885892343815 35.0 34.0 37.0 29.0 39.0 80-81 33.4034047864609 35.0 34.0 36.5 29.0 39.0 82-83 33.12664722029779 35.0 34.0 36.0 29.0 38.0 84-85 32.90036531169049 35.0 34.0 36.0 29.0 37.0 86-87 32.702070082163786 35.0 34.0 35.5 28.0 37.0 88-89 32.53970047785194 35.0 33.0 35.0 28.0 36.0 90-91 32.394650263168614 35.0 33.0 35.0 27.5 36.0 92-93 32.28300814741205 35.0 33.0 35.0 27.0 36.0 94-95 32.189059510486516 35.0 33.0 35.0 27.0 36.0 96-97 32.12131801657487 35.0 33.0 35.0 27.0 35.5 98-99 32.05556765946597 35.0 33.0 35.0 27.0 35.0 100-101 31.9977693102652 35.0 33.0 35.0 27.0 35.0 102-103 30.794184062337052 34.0 31.0 34.5 24.5 35.0 104-105 31.736045845781206 35.0 33.0 35.0 25.5 35.0 106-107 31.888244508046576 35.0 33.0 35.0 27.0 35.0 108-109 31.887046220915348 35.0 33.0 35.0 27.0 35.0 110-111 31.85057696467365 35.0 33.0 35.0 27.0 35.0 112-113 31.793819638847175 35.0 33.0 35.0 25.5 35.0 114-115 31.72240151683767 35.0 33.0 35.0 25.0 35.0 116-117 31.659320986299768 35.0 33.0 35.0 25.0 35.0 118-119 31.57682203907874 35.0 33.0 35.0 25.0 35.0 120-121 31.46466737931705 35.0 33.0 35.0 25.0 35.0 122-123 31.325424168043376 35.0 33.0 35.0 25.0 35.0 124-125 30.474423218191184 34.5 31.5 35.0 21.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.18530732651831983 1101 2 0.2335330963928115 1101 3 0.16394277981779481 1101 4 0.18159025372442272 1101 5 0.16737111822735784 1101 6 0.1604193703258474 1101 7 0.21217988295996548 1101 8 0.22679796887219084 1101 9 0.28188419921864494 1101 10-11 0.32266785717418855 1101 12-13 0.31736241618794736 1101 14-15 0.43568882897943695 1101 16-17 0.3856730423847452 1101 18-19 0.4422353163403585 1101 20-21 0.3202793698782642 1101 22-23 0.3655614959369302 1101 24-25 0.36643250739678024 1101 26-27 0.43302094766954013 1101 28-29 0.4445579741572132 1101 30-31 0.4909683001399259 1101 32-33 0.4668320425929835 1101 34-35 0.4591119122150502 1101 36-37 0.49123836772770346 1101 38-39 0.545181436950763 1101 40-41 0.5375480948061906 1101 42-43 0.5354162827924824 1101 44-45 0.6143197894400174 1101 46-47 0.5769282722441247 1101 48-49 0.5575907053631823 1101 50-51 0.6308007634427923 1101 52-53 0.5485084884292704 1101 54-55 0.6217586714707011 1101 56-57 0.5889449852143329 1101 58-59 0.5740212249916041 1101 60-61 0.6694282567630765 1101 62-63 0.720985142455703 1101 64-65 0.6574326114179669 1101 66-67 0.6122748131912417 1101 68-69 0.6231616887980138 1101 70-71 0.6350952342893663 1101 72-73 0.5891034823297403 1101 74-75 0.657617871777326 1101 76-77 0.5734812628351449 1101 78-79 0.5592403125603553 1101 80-81 0.4690608993276797 1101 82-83 0.47929107056187803 1101 84-85 0.466355884196588 1101 86-87 0.5180668885756816 1101 88-89 0.5728052020280217 1101 90-91 0.6269924219782652 1101 92-93 0.6015133666488737 1101 94-95 0.5646395048962276 1101 96-97 0.6118513539574906 1101 98-99 0.5965045363383368 1101 100-101 0.5309732925814217 1101 102-103 0.5662022952053682 1101 104-105 0.5426225015601602 1101 106-107 0.529427217210813 1101 108-109 0.5592836188551189 1101 110-111 0.5782036135424562 1101 112-113 0.49209280070719785 1101 114-115 0.5768494468240881 1101 116-117 0.5900473135451314 1101 118-119 0.5651249315401472 1101 120-121 0.5738021334873444 1101 122-123 0.5894314912608181 1101 124-125 0.6099771918540284 1102 1 0.30925354497735924 1102 2 0.34620541415601735 1102 3 0.23146730325364473 1102 4 0.21806423518535922 1102 5 0.18995239601342462 1102 6 0.24165709015636594 1102 7 0.2853155245653767 1102 8 0.34391131256299445 1102 9 0.32398537806219707 1102 10-11 0.37549795682840426 1102 12-13 0.35267843071512317 1102 14-15 0.44618987307391933 1102 16-17 0.42872447636059263 1102 18-19 0.5168763413190121 1102 20-21 0.4521407786598388 1102 22-23 0.5434960445775943 1102 24-25 0.5055697600866154 1102 26-27 0.4832246001286862 1102 28-29 0.496714592018634 1102 30-31 0.5458896200693388 1102 32-33 0.5890742598584779 1102 34-35 0.4900637383655493 1102 36-37 0.5482165089601381 1102 38-39 0.5809352805416736 1102 40-41 0.6231030833425066 1102 42-43 0.6713106745211235 1102 44-45 0.6962652582386966 1102 46-47 0.6270192384150448 1102 48-49 0.6243977268655669 1102 50-51 0.5811066375219909 1102 52-53 0.6541072704157003 1102 54-55 0.5894271961211857 1102 56-57 0.6720451260260631 1102 58-59 0.650385757094412 1102 60-61 0.7164266103570966 1102 62-63 0.666305142518965 1102 64-65 0.5715332377692093 1102 66-67 0.5654153734542859 1102 68-69 0.5192766609704336 1102 70-71 0.46677407810432214 1102 72-73 0.4531951201372877 1102 74-75 0.4322210447068855 1102 76-77 0.4941829333335903 1102 78-79 0.4981839820687384 1102 80-81 0.44577477210367533 1102 82-83 0.4612437354673844 1102 84-85 0.4053160283085049 1102 86-87 0.43839491506578554 1102 88-89 0.515392526652569 1102 90-91 0.4978414149714183 1102 92-93 0.43667085595092914 1102 94-95 0.4761971114447334 1102 96-97 0.43850715705091403 1102 98-99 0.43416748334705346 1102 100-101 0.505370440708937 1102 102-103 0.530250492778304 1102 104-105 0.47070097233738295 1102 106-107 0.5207300763057638 1102 108-109 0.5792623046720422 1102 110-111 0.5376888731215814 1102 112-113 0.4720153990658602 1102 114-115 0.5484658136705427 1102 116-117 0.5582733387794825 1102 118-119 0.5804626556408792 1102 120-121 0.5972924139984421 1102 122-123 0.5751175649225928 1102 124-125 0.7287315378544434 1103 1 0.16382709661500883 1103 2 0.20833084278105218 1103 3 0.06269085506365712 1103 4 0.060480175924666923 1103 5 0.07329276331977752 1103 6 0.11599454653054408 1103 7 0.07637648571962785 1103 8 0.17132273005547916 1103 9 0.27434342124209365 1103 10-11 0.1546125725050942 1103 12-13 0.15441715990191085 1103 14-15 0.02217672635672585 1103 16-17 0.20244985396675474 1103 18-19 0.16506980036430008 1103 20-21 0.21370805290746375 1103 22-23 0.28088019947405485 1103 24-25 0.24614345650756775 1103 26-27 0.24847559969975208 1103 28-29 0.15721524674445675 1103 30-31 0.1754064033587852 1103 32-33 0.2965942746716834 1103 34-35 0.2091911629317309 1103 36-37 0.14274510390719541 1103 38-39 0.13079231934900548 1103 40-41 0.17788139992644147 1103 42-43 0.18602366133649184 1103 44-45 0.133059101107456 1103 46-47 0.11668742366477147 1103 48-49 0.19128797866538605 1103 50-51 0.2434482315466795 1103 52-53 0.2749322544969246 1103 54-55 0.27774852737896083 1103 56-57 0.3856952852630684 1103 58-59 0.3728624034011361 1103 60-61 0.2420366331038295 1103 62-63 0.19517648286262812 1103 64-65 0.2893288037561703 1103 66-67 0.2386120211792928 1103 68-69 0.2571929191003335 1103 70-71 0.19625326205839144 1103 72-73 0.15752694769151532 1103 74-75 0.15562043829140748 1103 76-77 0.17511169847488617 1103 78-79 0.12283513558286074 1103 80-81 0.08929527642230539 1103 82-83 0.15105352764425817 1103 84-85 0.14532253370759918 1103 86-87 0.03241258256750257 1103 88-89 0.0677858585446387 1103 90-91 0.022476366545348014 1103 92-93 0.10261775101406556 1103 94-95 0.11707482816292725 1103 96-97 0.10016509321921063 1103 98-99 0.1438608480862129 1103 100-101 0.0748712239668663 1103 102-103 0.10423869638695393 1103 104-105 0.14279093890425543 1103 106-107 0.20594090589927205 1103 108-109 0.15482896629920617 1103 110-111 0.11749869484606634 1103 112-113 0.1926410106398535 1103 114-115 0.17533771640374596 1103 116-117 0.21350639310128727 1103 118-119 0.24252588654413643 1103 120-121 0.1513549396987095 1103 122-123 0.19111988637991928 1103 124-125 0.37366417176298583 1104 1 0.24281370206288244 1104 2 0.23194245738993402 1104 3 0.20068976824381224 1104 4 0.23652856857596305 1104 5 0.1980275231470543 1104 6 0.2210060017685933 1104 7 0.14977616555632522 1104 8 0.22921292203335497 1104 9 0.19344078721139368 1104 10-11 0.21555175495871026 1104 12-13 0.16367485998620168 1104 14-15 0.24272118780366725 1104 16-17 0.28893604333959644 1104 18-19 0.2646803688416668 1104 20-21 0.32380346706206353 1104 22-23 0.34630851899909487 1104 24-25 0.36014151964459273 1104 26-27 0.4040816561835072 1104 28-29 0.45950294859599694 1104 30-31 0.39235392986319795 1104 32-33 0.37153349340164965 1104 34-35 0.3105239388838399 1104 36-37 0.393735043837971 1104 38-39 0.28316052773605094 1104 40-41 0.33997896222923885 1104 42-43 0.4221361920457696 1104 44-45 0.3268802091881895 1104 46-47 0.3062195273768964 1104 48-49 0.4008607437542082 1104 50-51 0.3349409249259736 1104 52-53 0.3935418873876202 1104 54-55 0.33801053086786226 1104 56-57 0.29848130161242636 1104 58-59 0.35088543054981614 1104 60-61 0.4009615466962586 1104 62-63 0.4990562998043231 1104 64-65 0.37614809826450113 1104 66-67 0.3480947204240863 1104 68-69 0.27044267221280904 1104 70-71 0.19249271587382566 1104 72-73 0.3312171324631521 1104 74-75 0.28188719986485467 1104 76-77 0.31100345817936415 1104 78-79 0.30796481005408793 1104 80-81 0.3108673635355501 1104 82-83 0.23274701062243963 1104 84-85 0.2548797465367727 1104 86-87 0.3473116472994633 1104 88-89 0.3914810803365256 1104 90-91 0.34195237917637655 1104 92-93 0.3710649491929985 1104 94-95 0.46401387629600066 1104 96-97 0.34038861681464994 1104 98-99 0.36034548345865147 1104 100-101 0.3235812688147277 1104 102-103 0.42248161949604324 1104 104-105 0.43589343209447406 1104 106-107 0.46772606264619654 1104 108-109 0.4321030829026711 1104 110-111 0.35817260893925607 1104 112-113 0.40265773207645594 1104 114-115 0.4248518461647386 1104 116-117 0.42739089655526286 1104 118-119 0.3980942572923567 1104 120-121 0.38965109835185885 1104 122-123 0.45756851502357776 1104 124-125 0.493500276192794 1105 1 0.12711722305082418 1105 2 0.13877799691606185 1105 3 0.1735270904194195 1105 4 0.14128187920202606 1105 5 0.09516908578734729 1105 6 0.16571421384214347 1105 7 0.15562543858097655 1105 8 0.12595743559779748 1105 9 0.17085383565137846 1105 10-11 0.14317427808101257 1105 12-13 0.23528764975485217 1105 14-15 0.2437805322388158 1105 16-17 0.25563514722644953 1105 18-19 0.26297659443262944 1105 20-21 0.2582742056268188 1105 22-23 0.28671269235312025 1105 24-25 0.264391621914811 1105 26-27 0.2584523315752847 1105 28-29 0.2852050278594902 1105 30-31 0.2543898278637542 1105 32-33 0.3010438530160613 1105 34-35 0.333378081259049 1105 36-37 0.33318469517126914 1105 38-39 0.31118821835424626 1105 40-41 0.22138288255107597 1105 42-43 0.2548629530076454 1105 44-45 0.19294298936786447 1105 46-47 0.2305814353611737 1105 48-49 0.22190263115560782 1105 50-51 0.21532888817724682 1105 52-53 0.25116704048911487 1105 54-55 0.2921291827920669 1105 56-57 0.1906449237500425 1105 58-59 0.18384419845560984 1105 60-61 0.1210006269230206 1105 62-63 0.18579618538321085 1105 64-65 0.22662503113512855 1105 66-67 0.291688108141102 1105 68-69 0.1671404562487382 1105 70-71 0.21251221709656676 1105 72-73 0.2258331129653257 1105 74-75 0.19744949403312972 1105 76-77 0.16443407400527832 1105 78-79 0.2686904339510221 1105 80-81 0.2926927975732596 1105 82-83 0.23326200770199534 1105 84-85 0.2525760233175376 1105 86-87 0.28909181993013533 1105 88-89 0.2035865075003187 1105 90-91 0.29760952031121946 1105 92-93 0.31556236187196873 1105 94-95 0.3494632733817795 1105 96-97 0.3398230011430883 1105 98-99 0.30347236585652837 1105 100-101 0.3455097533123137 1105 102-103 0.2695310113315834 1105 104-105 0.3352176708528276 1105 106-107 0.2757220257982915 1105 108-109 0.2527053238296588 1105 110-111 0.32073575456936965 1105 112-113 0.26689447541357225 1105 114-115 0.2994629094322683 1105 116-117 0.32083086071753897 1105 118-119 0.30496364382419117 1105 120-121 0.26344175152250315 1105 122-123 0.24495165170398892 1105 124-125 0.2822586221838108 1106 1 0.06375683165599355 1106 2 0.12024296085624542 1106 3 0.0871584195186017 1106 4 0.10953554433662305 1106 5 0.05721824488838223 1106 6 0.11060631384456343 1106 7 0.13739920722802879 1106 8 0.12682190246952985 1106 9 0.15476688034468822 1106 10-11 0.1381424614150646 1106 12-13 0.11040304397224077 1106 14-15 0.1920668058463093 1106 16-17 0.20558805800191493 1106 18-19 0.20928963856702154 1106 20-21 0.2729682793710566 1106 22-23 0.22526649423743805 1106 24-25 0.30644239257584616 1106 26-27 0.3040455067583778 1106 28-29 0.2900081520502127 1106 30-31 0.3830395920915919 1106 32-33 0.34285160881162113 1106 34-35 0.38076306897207246 1106 36-37 0.2779951691934883 1106 38-39 0.2818105506635078 1106 40-41 0.3606428597842992 1106 42-43 0.3864890962371277 1106 44-45 0.36362151668147646 1106 46-47 0.3355813235766618 1106 48-49 0.3331720001506824 1106 50-51 0.4458670886702123 1106 52-53 0.5265168561378246 1106 54-55 0.4064401384192067 1106 56-57 0.44334801291495296 1106 58-59 0.4125285227275626 1106 60-61 0.4721051743168587 1106 62-63 0.48358208358106225 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-0.6115321307114812 2216 82-83 -0.653692610723489 2216 84-85 -0.7676997412302029 2216 86-87 -0.7731046149498972 2216 88-89 -0.7544383637194123 2216 90-91 -0.7911262151827003 2216 92-93 -0.7195690389156724 2216 94-95 -0.7889108719813152 2216 96-97 -0.8334266601207148 2216 98-99 -0.9436338890918599 2216 100-101 -0.854319237851918 2216 102-103 -0.853761285776951 2216 104-105 -0.8253360738171054 2216 106-107 -1.0002495338590727 2216 108-109 -0.932985881875716 2216 110-111 -0.8715202541290843 2216 112-113 -1.0204004850388841 2216 114-115 -1.0115250831781388 2216 116-117 -0.9673077402216599 2216 118-119 -0.9991595588724778 2216 120-121 -1.2497762912594972 2216 122-123 -1.332623243789321 2216 124-125 -1.6212649790369866 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10.0 3 10.0 4 22.0 5 100.0 6 276.0 7 639.0 8 1328.0 9 2373.0 10 3738.0 11 5475.0 12 6787.0 13 9423.0 14 13691.0 15 12456.0 16 5829.0 17 4589.0 18 4458.0 19 4226.0 20 4160.0 21 4281.0 22 4686.0 23 5850.0 24 7328.0 25 9966.0 26 13558.0 27 17936.0 28 23491.0 29 30367.0 30 38784.0 31 49249.0 32 61947.0 33 80803.0 34 107759.0 35 154927.0 36 282883.0 37 607057.0 38 331346.0 39 2174.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.98578762078063 19.63011284808083 13.233783354502487 24.150316176636053 2 16.113437025031573 20.68133690501755 39.164802713560434 24.04042335639045 3 18.92927937671305 24.728288980774114 30.524372747497104 25.81805889501573 4 12.610933645143998 16.785058584667986 37.39204443928939 33.21196333089862 5 12.5964611997396 38.53594234428537 35.331418999760714 13.536177456214322 6 31.94857631889955 37.49883750212907 16.878110661437777 13.674475517533601 7 27.53354002284243 33.148274121700204 21.42820569890417 17.889980156553197 8 25.76670000031348 36.38816874975836 19.683361703506094 18.161769546422065 9 26.024696157017154 15.066390384026601 20.276313988323817 38.63259947063243 10-11 24.594912880080212 26.83724048065514 28.301055763122847 20.2667908761418 12-13 25.212186983705337 24.304840747424862 28.676227011335097 21.8067452575347 14-15 22.124822000630623 25.741941601838892 26.564154355723918 25.569082041806567 16-17 21.639310858292298 29.026319570527303 27.31467234420512 22.019697226975275 18-19 21.40623579532096 27.429437685411877 29.78583393156258 21.378492587704585 20-21 22.15480605355745 26.607538628889927 29.969795954193927 21.267859363358692 22-23 21.81271819693184 26.849312062495883 29.648894294721686 21.689075445850587 24-25 21.624471912484026 26.82689805860243 29.963578549850524 21.585051479063022 26-27 21.59437758557813 27.107569454676167 29.710754855583804 21.5872981041619 28-29 21.438916353445332 27.061853246268775 29.895474461097336 21.60375593918856 30-31 21.60618542912107 26.901820393295235 29.92697945957695 21.565014718006754 32-33 21.43855062377807 26.936460217494208 29.811252143437084 21.813737015290634 34-35 21.82834007843334 27.065118689726447 29.58763457545578 21.518906656384438 36-37 21.904124490198967 26.79897198615243 29.474937590844636 21.821965932803966 38-39 21.65320781491153 27.051534444942533 29.62308422963225 21.672173510513684 40-41 21.991011409720677 26.838418543121094 29.520601552156705 21.64996849500152 42-43 21.79012132820476 26.824964916075487 29.446880900656332 21.938032855063422 44-45 21.884949806215523 27.064543971677896 29.187212836902333 21.863293385204248 46-47 22.047281531383263 27.14202641404151 28.69120503745594 22.119487017119283 48-49 22.069146940775827 27.409557665641575 28.432921521727998 22.088373871854593 50-51 22.083958992299824 27.45044101773162 28.266566770220408 22.19903321974815 52-53 22.213296676771254 27.452713766378157 28.227564313561988 22.106425243288598 54-55 22.07836855310029 27.338788975026933 28.35193852397776 22.230903947895015 56-57 22.115307249493465 27.643023811091222 28.125734724777974 22.11593421463734 58-59 22.09600194777171 27.417420853487652 28.26003588330507 22.22654131543557 60-61 22.152742293292206 27.33296342389845 28.422132496543856 22.092161786265493 62-63 22.091038473716054 27.30569044013998 28.5226559079448 22.080615178199167 64-65 21.899474498715243 27.30323482665981 28.38229408636027 22.414996588264678 66-67 21.798036763146154 27.6838810396336 28.118132772408515 22.399949424811727 68-69 21.930195790765012 27.407389411185683 28.230072174137476 22.432342623911826 70-71 21.829646255816407 27.311724979649753 28.297941150961712 22.56068761357212 72-73 21.98573445309308 27.253782950936845 28.24402214858865 22.51646044738143 74-75 21.84806335691767 27.40576975123068 28.01267201049958 22.73349488135207 76-77 21.9108643654956 27.346469298039377 28.042243866452242 22.70042247001278 78-79 21.961909777625912 27.337273809262573 28.06815842573232 22.632657987379194 80-81 21.760027001298862 27.490201057272223 27.958204413625626 22.79156752780329 82-83 21.91008065906576 27.450127535159684 27.938533382236614 22.701258423537944 84-85 21.868309106355234 27.31015756679007 27.908595796616687 22.912937530238008 86-87 21.8963919200912 27.15827526068688 27.985346779645788 22.95998603957613 88-89 21.858565023077542 27.242941678657374 27.879076187759345 23.019417110505742 90-91 21.818700489346295 27.331604999420055 27.988220369888534 22.861474141345113 92-93 21.90206072993372 27.10187452128599 28.008831848993353 22.987232899786935 94-95 21.721399268118866 27.070719947836224 27.94466167739131 23.263219106653597 96-97 21.854031272892193 26.979557564756497 27.838449167181334 23.32796199516998 98-99 21.806625953169466 26.854737352487156 27.769654642365353 23.568982051978026 100-101 21.616994062695035 26.934313453232395 27.940052734825542 23.508639749247028 102-103 21.787846250708633 26.82453844124155 27.826213036765342 23.561402271284475 104-105 21.70077763390697 26.702047389580898 27.899824115325167 23.697350861186965 106-107 21.5803783531261 26.831305632865153 27.881229809652908 23.707086204355836 108-109 21.657745906222793 26.72632653850455 27.63157207106925 23.984355484203405 110-111 21.562566299448285 26.772932162665796 27.721504170529236 23.942997367356686 112-113 21.562520250595238 26.74061051755818 27.73539814499322 23.96147108685337 114-115 21.505252997289233 26.821407499115484 27.540307471364983 24.133032032230297 116-117 21.363598474723 26.755504409877563 27.651093800668257 24.229803314731182 118-119 21.367367057181475 26.74572242191119 27.480122622990738 24.406787897916598 120-121 21.25620476501914 26.859613508822218 27.51561953322869 24.368562192929947 122-123 21.371615345725605 26.872682000896177 27.395205865101108 24.360496788277114 124-125 21.237550993960934 26.775363208683462 27.3530639747882 24.634021822567405 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 157.0 1 117.5 2 131.0 3 192.5 4 147.5 5 124.0 6 271.5 7 598.0 8 947.0 9 1138.0 10 1039.0 11 825.0 12 889.0 13 1119.5 14 1301.5 15 1477.5 16 1662.5 17 1862.5 18 2154.5 19 2492.5 20 2900.5 21 3489.0 22 4254.0 23 5293.0 24 6720.5 25 8588.5 26 11275.5 27 14662.0 28 17718.0 29 20920.5 30 24833.5 31 28866.5 32 33106.5 33 36948.0 34 41693.0 35 46097.0 36 50546.5 37 55500.0 38 58227.0 39 60046.0 40 61689.0 41 64063.0 42 66800.5 43 69483.0 44 71414.0 45 72992.5 46 73117.5 47 73086.0 48 74443.0 49 73641.0 50 71135.0 51 67782.0 52 64665.0 53 62380.5 54 59340.0 55 54192.0 56 48932.5 57 44294.0 58 39385.0 59 33932.0 60 28060.5 61 22247.0 62 17090.5 63 12700.5 64 9447.0 65 7493.5 66 5608.0 67 4296.5 68 3103.5 69 1941.5 70 1345.5 71 997.0 72 742.0 73 601.0 74 481.0 75 381.0 76 225.0 77 86.0 78 51.0 79 36.5 80 13.0 81 3.0 82 2.0 83 1.0 84 1.5 85 1.0 86 0.5 87 1.0 88 1.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16630250441226718 2 0.002037636717586686 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 5.747180485500908E-4 12-13 0.005433697913564495 14-15 2.6123547661367767E-5 16-17 3.657296672591487E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 5.224709532273553E-4 96-97 0.006269651438728264 98-99 0.002011513169925318 100-101 0.003474431838961913 102-103 0.0045454972930779915 104-105 0.011912337733583703 106-107 0.01671907050327537 108-109 0.024451640611040226 110-111 0.01651008212198443 112-113 0.023929169657812874 114-115 0.026280288947335972 116-117 0.025783941541769984 118-119 0.01627497019303212 120-121 0.02259686872708312 122-123 0.015125534095931936 124-125 0.01436795121375227 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1913982.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.99430718627866 #Duplication Level Percentage of deduplicated Percentage of total 1 84.94747542609026 61.15734640524801 2 9.884193755576993 14.23211363055415 3 2.6026875410985673 5.621360590312516 4 0.9974462770379812 2.8724181468352974 5 0.46742656016519385 1.6826025679779266 6 0.2680767825803945 1.1580001340761297 7 0.1604259497899407 0.8084828576879257 8 0.10394917661558298 0.5986999162418412 9 0.07908883060970733 0.5124551009326946 >10 0.431876734014648 5.756849202504631 >50 0.03921349241518433 1.92848995932054 >100 0.017170190009745383 2.1156202943820115 >500 4.1540742677803475E-4 0.19130491740224773 >1k 5.538765690373796E-4 1.3642562765241064 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4916 0.2568467206065679 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4710 0.24608381897008436 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2611 0.13641716588766248 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 5.2247095322735534E-5 0.0 22-23 0.0 0.0 0.0 1.567412859682066E-4 0.0 24-25 0.0 0.0 0.0 3.657296672591487E-4 0.0 26-27 0.0 0.0 0.0 6.79212239195562E-4 0.0 28-29 0.0 0.0 0.0 0.002089883812909421 0.0 30-31 0.0 0.0 0.0 0.0071317285115534 0.0 32-33 0.0 0.0 0.0 0.018495471744248378 0.0 34-35 0.0 0.0 0.0 0.03573701320075111 0.0 36-37 0.0 0.0 0.0 0.06596195784495361 0.0 38-39 0.0 0.0 0.0 0.1116781662523472 0.0 40-41 0.0 0.0 0.0 0.16188762485749605 0.0 42-43 0.0 0.0 0.0 0.21251506022522676 0.0 44-45 0.0 0.0 0.0 0.2723118608220976 0.0 46-47 0.0 0.0 0.0 0.3466594774663503 0.0 48-49 0.0 0.0 0.0 0.43012421224442027 0.0 50-51 0.0 0.0 0.0 0.5229934241805827 0.0 52-53 0.0 2.6123547661367767E-5 0.0 0.6112910152760057 0.0 54-55 0.0 5.2247095322735534E-5 0.0 0.7136953221085673 0.0 56-57 0.0 5.2247095322735534E-5 0.0 0.8362147606403822 0.0 58-59 0.0 5.2247095322735534E-5 0.0 0.9770729296304772 0.0 60-61 0.0 5.2247095322735534E-5 0.0 1.128067035113183 0.0 62-63 0.0 5.2247095322735534E-5 0.0 1.278512546095 0.0 64-65 0.0 7.83706429841033E-5 0.0 1.4350970907772382 0.0 66-67 0.0 1.0449419064547107E-4 0.0 1.6177529360255216 0.0 68-69 0.0 1.0449419064547107E-4 0.0 1.8363547828558473 0.0 70-71 0.0 1.0449419064547107E-4 0.0 2.079434393844874 0.0 72-73 0.0 1.0449419064547107E-4 0.0 2.3241075412412444 0.0 74-75 0.0 1.0449419064547107E-4 0.0 2.569747259901086 0.0 76-77 0.0 1.0449419064547107E-4 0.0 2.845507429014484 0.0 78-79 0.0 1.0449419064547107E-4 0.0 3.1610015141208225 0.0 80-81 0.0 1.0449419064547107E-4 0.0 3.5165168742443766 0.0 82-83 0.0 1.0449419064547107E-4 0.0 3.8888819226095124 0.0 84-85 0.0 1.0449419064547107E-4 0.0 4.2489166564784835 0.0 86-87 0.0 1.0449419064547107E-4 0.0 4.623789565419111 0.0 88-89 0.0 1.0449419064547107E-4 0.0 5.05255535318514 0.0 90-91 0.0 1.0449419064547107E-4 0.0 5.529127233171472 0.0 92-93 0.0 1.0449419064547107E-4 0.0 6.032031649200462 0.0 94-95 0.0 1.0449419064547107E-4 0.0 6.534700953300501 5.2247095322735534E-5 96-97 0.0 1.0449419064547107E-4 0.0 7.040923059882486 5.2247095322735534E-5 98-99 0.0 1.0449419064547107E-4 0.0 7.586800711814426 5.2247095322735534E-5 100-101 0.0 1.0449419064547107E-4 0.0 8.204021772409563 5.2247095322735534E-5 102-103 0.0 1.0449419064547107E-4 0.0 8.844884643638236 5.2247095322735534E-5 104-105 0.0 1.0449419064547107E-4 0.0 9.486505097749092 5.2247095322735534E-5 106-107 0.0 1.3061773830683882E-4 0.0 10.119922757894274 5.2247095322735534E-5 108-109 0.0 1.567412859682066E-4 0.0 10.770634206591284 5.2247095322735534E-5 110-111 0.0 2.0898838129094214E-4 0.0 11.487046377656634 5.2247095322735534E-5 112-113 0.0 2.0898838129094214E-4 0.0 12.25724693335674 5.2247095322735534E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3545 0.0 75.98199 1 GTATCAA 6270 0.0 48.091846 1 ATCAACG 7955 0.0 37.39409 3 TCAACGC 8015 0.0 37.115124 4 CAACGCA 8195 0.0 36.299908 5 TATCAAC 8305 0.0 35.603268 2 AACGCAG 8415 0.0 35.562996 6 TGGTATC 1440 0.0 33.05222 2 GTACATG 7290 0.0 32.53449 1 TACATGG 7645 0.0 30.583569 2 ACGCAGA 9825 0.0 30.464071 7 CGCAGAG 9810 0.0 30.146711 8 ACATGGG 7615 0.0 29.45402 3 GTGGTAT 1610 0.0 29.240828 1 GCAGAGT 10930 0.0 27.003126 9 GAGTACT 7520 0.0 26.191639 12-13 CAGAGTA 10760 0.0 25.494188 10-11 GAGTACA 5615 0.0 24.834452 1 AGAGTAC 10425 0.0 24.172964 10-11 CATGGGG 2995 0.0 24.036598 4 >>END_MODULE