FastQCFastQC Report
Thu 26 May 2016
SRR1364095_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364095_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2290562
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN91640.4000764877789818No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25400.048.03421
GTATCAA41150.047.004991
ATCAACG45600.040.9848943
TCAACGC46600.040.110674
CAACGCA46650.040.070325
GTACATG93900.037.585431
AACGCAG50700.036.8718646
TACATGG95900.035.4362682
TATCAAC54500.034.944912
ACATGGG96450.034.064023
ACGCAGA61450.030.2297577
CATGGGA70650.029.9953584
CGCAGAG61150.029.793878
ATGGGAG34300.023.6042165
GCAGAGT76000.023.3455779
CATGGGG36750.023.0010244
TAGCCCT22800.022.975454
GAGTACA68750.022.421131
GGATACG1954.0330866E-521.3645551
AGAGTAC63900.021.29057310-11
Produced by FastQC (version 0.11.5)