##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364095_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2290562 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.758921609631173 31.0 31.0 34.0 27.0 34.0 2 30.765907231500393 31.0 31.0 34.0 27.0 34.0 3 30.82763967969433 31.0 31.0 34.0 27.0 34.0 4 34.303615444593945 37.0 35.0 37.0 30.0 37.0 5 34.33931279747066 37.0 35.0 37.0 30.0 37.0 6 34.38923067788604 37.0 35.0 37.0 30.0 37.0 7 34.25892859481647 37.0 35.0 37.0 30.0 37.0 8 34.23117252447216 37.0 35.0 37.0 30.0 37.0 9 35.69924367906217 39.0 35.0 39.0 30.0 39.0 10-11 35.62377792000392 39.0 35.0 39.0 30.0 39.0 12-13 35.533942979932434 39.0 35.0 39.0 30.0 39.0 14-15 36.74526884668479 40.0 37.0 41.0 30.0 41.0 16-17 36.76189642541874 40.0 37.0 41.0 30.0 41.0 18-19 36.78202860258749 40.0 37.0 41.0 30.0 41.0 20-21 36.81026446784675 40.0 37.0 41.0 30.0 41.0 22-23 36.76611394932772 40.0 37.0 41.0 30.0 41.0 24-25 36.78168501878578 40.0 37.0 41.0 30.0 41.0 26-27 36.75024404491125 40.0 37.0 41.0 30.0 41.0 28-29 36.71022351719796 40.0 37.0 41.0 30.0 41.0 30-31 36.666275350765446 40.0 37.0 41.0 30.0 41.0 32-33 36.57151825621834 40.0 36.0 41.0 30.0 41.0 34-35 36.54680903638496 40.0 36.0 41.0 30.0 41.0 36-37 36.4881302056002 40.0 36.0 41.0 30.0 41.0 38-39 36.41362098035329 40.0 36.0 41.0 30.0 41.0 40-41 36.3483005480751 40.0 36.0 41.0 29.5 41.0 42-43 36.244873528854484 40.0 36.0 41.0 29.0 41.0 44-45 36.153869879968326 40.0 36.0 41.0 29.0 41.0 46-47 36.05781877984529 40.0 35.0 41.0 28.0 41.0 48-49 35.96765051546302 40.0 35.0 41.0 28.0 41.0 50-51 35.86092583392198 40.0 35.0 41.0 28.0 41.0 52-53 35.73423902081672 39.5 35.0 41.0 28.0 41.0 54-55 35.576418363702885 39.0 35.0 41.0 27.0 41.0 56-57 35.415639044042464 39.0 35.0 41.0 27.0 41.0 58-59 35.228437824429115 39.0 34.5 41.0 26.5 41.0 60-61 35.01250893885431 38.5 34.0 41.0 26.0 41.0 62-63 34.77197037233657 38.0 34.0 40.0 26.0 41.0 64-65 34.51271631154275 38.0 34.0 40.0 26.0 41.0 66-67 34.22171283728622 37.0 34.0 40.0 26.0 41.0 68-69 33.90705621589811 37.0 33.5 39.5 25.5 41.0 70-71 33.56601305705762 36.0 33.0 39.0 25.0 41.0 72-73 33.208477439161214 36.0 33.0 39.0 24.0 40.5 74-75 32.930503081776436 35.0 33.0 38.5 24.5 40.0 76-77 32.69074139883574 35.0 33.0 37.0 24.0 39.5 78-79 32.36699181248969 35.0 33.0 37.0 24.0 39.0 80-81 32.1348747599934 35.0 32.5 36.5 23.5 39.0 82-83 32.00068694931637 35.0 33.0 36.0 24.0 38.0 84-85 31.75577412879459 35.0 33.0 36.0 24.0 37.0 86-87 31.535021317912374 35.0 33.0 35.5 23.5 37.0 88-89 31.332558778151387 35.0 32.0 35.0 23.0 36.0 90-91 31.174102032601606 35.0 32.0 35.0 22.0 36.0 92-93 31.04047390989635 35.0 32.0 35.0 20.5 36.0 94-95 30.92519805183182 35.0 32.0 35.0 20.0 36.0 96-97 30.82471856251872 35.0 32.0 35.0 20.0 35.5 98-99 30.743816364717482 35.0 32.0 35.0 19.5 35.0 100-101 30.666077146132693 35.0 32.0 35.0 19.0 35.0 102-103 29.57548627804006 33.5 30.0 34.5 16.5 35.0 104-105 30.389573606826623 34.5 31.5 35.0 18.0 35.0 106-107 30.48840371926191 35.0 32.0 35.0 17.0 35.0 108-109 30.468815295110982 35.0 32.0 35.0 16.0 35.0 110-111 30.396984233563643 35.0 32.0 35.0 10.5 35.0 112-113 30.31777899921504 35.0 32.0 35.0 7.0 35.0 114-115 30.216409117063847 35.0 32.0 35.0 2.0 35.0 116-117 30.118780674786365 35.0 32.0 35.0 2.0 35.0 118-119 29.99142153759645 35.0 32.0 35.0 2.0 35.0 120-121 29.845989106603533 35.0 32.0 35.0 2.0 35.0 122-123 29.680566821592258 35.0 32.0 35.0 2.0 35.0 124-125 28.83745473818216 34.5 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4547198327743196 1101 2 0.49165718529946645 1101 3 0.5870549850882547 1101 4 0.5397189501285382 1101 5 0.498835570507417 1101 6 0.44672730051450316 1101 7 0.4510887222914022 1101 8 0.4737526152834235 1101 9 0.359417108996233 1101 10-11 0.4773265566275313 1101 12-13 0.5652506656726999 1101 14-15 0.636493000318822 1101 16-17 0.6307372842264627 1101 18-19 0.6393662754187517 1101 20-21 0.5876173714887756 1101 22-23 0.7274988297054605 1101 24-25 0.6700582522342486 1101 26-27 0.597694466479382 1101 28-29 0.6439304068435305 1101 30-31 0.6769769486394139 1101 32-33 0.6826185571322725 1101 34-35 0.7131884177938943 1101 36-37 0.7693653459692555 1101 38-39 0.7083473133987539 1101 40-41 0.7296792330206472 1101 42-43 0.6678452071543717 1101 44-45 0.7145376391370633 1101 46-47 0.6983426132255985 1101 48-49 0.6185032314796359 1101 50-51 0.6880988328182767 1101 52-53 0.7876100340822703 1101 54-55 0.7974328837923608 1101 56-57 0.6653799437259451 1101 58-59 0.6326323944626822 1101 60-61 0.567380779944429 1101 62-63 0.6617580498595785 1101 64-65 0.7689279816468115 1101 66-67 0.7400578269794309 1101 68-69 0.6968724372374311 1101 70-71 0.660715786996235 1101 72-73 0.7622091713476706 1101 74-75 0.731076277532388 1101 76-77 0.7196143030455744 1101 78-79 0.7193646823528113 1101 80-81 0.6215312142203402 1101 82-83 0.6251878260766119 1101 84-85 0.5750848184574657 1101 86-87 0.5561818780677896 1101 88-89 0.6456191808611251 1101 90-91 0.6836683036728424 1101 92-93 0.7039616199537271 1101 94-95 0.671289564314371 1101 96-97 0.5804213259214812 1101 98-99 0.5523352076228107 1101 100-101 0.638003646280378 1101 102-103 0.7877048950177432 1101 104-105 0.6511356498298149 1101 106-107 0.6781292237005943 1101 108-109 0.6580891887899405 1101 110-111 0.6332011466245184 1101 112-113 0.6471046410188386 1101 114-115 0.6248082212518007 1101 116-117 0.6717889036398468 1101 118-119 0.6406699725688263 1101 120-121 0.6451240764464821 1101 122-123 0.7083270066862326 1101 124-125 0.8327173734855897 1102 1 0.33693045246904063 1102 2 0.43142610795371183 1102 3 0.4400072709880618 1102 4 0.5061381399221787 1102 5 0.38252765212750717 1102 6 0.4260964053166987 1102 7 0.3726101511623341 1102 8 0.3996368119127851 1102 9 0.5547371353141557 1102 10-11 0.5533137075702967 1102 12-13 0.578736921491867 1102 14-15 0.5836320040855085 1102 16-17 0.5902561278941434 1102 18-19 0.48125461782927914 1102 20-21 0.5874742379884381 1102 22-23 0.6436912318445138 1102 24-25 0.6969064387526416 1102 26-27 0.6400137537487467 1102 28-29 0.7273711118713564 1102 30-31 0.6998651945922845 1102 32-33 0.7276740323639643 1102 34-35 0.7304749154991796 1102 36-37 0.6821674081269791 1102 38-39 0.7231393712236596 1102 40-41 0.7322657304325872 1102 42-43 0.7482309739383908 1102 44-45 0.8400742603478406 1102 46-47 0.9031007365909716 1102 48-49 0.7647826922623508 1102 50-51 0.8114154180653728 1102 52-53 0.8154704220629156 1102 54-55 0.7375893194765908 1102 56-57 0.7639175662672812 1102 58-59 0.7548835884928664 1102 60-61 0.8073082883051015 1102 62-63 0.8312452833694195 1102 64-65 0.8329638735583202 1102 66-67 0.8454141344905821 1102 68-69 0.8636785706330201 1102 70-71 0.8107652064471011 1102 72-73 0.6934947379518519 1102 74-75 0.7375391562602616 1102 76-77 0.7672298317965982 1102 78-79 0.6964443413655061 1102 80-81 0.6682664158643021 1102 82-83 0.6580443410838868 1102 84-85 0.6740679752939904 1102 86-87 0.6408377080013778 1102 88-89 0.6573050087810088 1102 90-91 0.6585577785549397 1102 92-93 0.6611701819426123 1102 94-95 0.6810635376226877 1102 96-97 0.7332368283062607 1102 98-99 0.7418258870349987 1102 100-101 0.7093578962582505 1102 102-103 0.7840921761352178 1102 104-105 0.7683052163518589 1102 106-107 0.7341310900441016 1102 108-109 0.7286132385827955 1102 110-111 0.7355569135241495 1102 112-113 0.7155358702465797 1102 114-115 0.8181003504277555 1102 116-117 0.8137286326162645 1102 118-119 0.7927591091071413 1102 120-121 0.8137156686863172 1102 122-123 0.8234452518677955 1102 124-125 0.8564669224727055 1103 1 0.27752438504129273 1103 2 0.3867555562707352 1103 3 0.3285327169507575 1103 4 0.2954728123945358 1103 5 0.3933853734211752 1103 6 0.32810093548575736 1103 7 0.3910209054047016 1103 8 0.24691933809159394 1103 9 0.39779169284890514 1103 10-11 0.4185735044950576 1103 12-13 0.3985184791677767 1103 14-15 0.4997699274949383 1103 16-17 0.4500289454010229 1103 18-19 0.521973995813994 1103 20-21 0.517027839400626 1103 22-23 0.5850088548830641 1103 24-25 0.49921707257910697 1103 26-27 0.5766690219144337 1103 28-29 0.6874060312796999 1103 30-31 0.5694848450646219 1103 32-33 0.30901056463699206 1103 34-35 0.5412660323373402 1103 36-37 0.5730594315319948 1103 38-39 0.7437939313032516 1103 40-41 0.7120840731638012 1103 42-43 0.713490951409625 1103 44-45 0.7306867376226265 1103 46-47 0.7255068812238363 1103 48-49 0.7276249804430748 1103 50-51 0.6840120650249517 1103 52-53 0.6000095221539326 1103 54-55 0.6381300045937266 1103 56-57 0.7050331921866686 1103 58-59 0.7839410825730866 1103 60-61 0.7364680834587887 1103 62-63 0.8440540242950263 1103 64-65 0.7621159739930619 1103 66-67 0.7187200711854871 1103 68-69 0.7822634146675185 1103 70-71 0.7288877655232042 1103 72-73 0.7873198420626935 1103 74-75 0.7125807980158143 1103 76-77 0.6343411328540611 1103 78-79 0.6483653300999208 1103 80-81 0.6653372641937381 1103 82-83 0.596552188063697 1103 84-85 0.5318448084984588 1103 86-87 0.6359156671108721 1103 88-89 0.5028406618098806 1103 90-91 0.499527239204145 1103 92-93 0.5444942988081145 1103 94-95 0.5605332880919818 1103 96-97 0.5548992933984636 1103 98-99 0.5778712434053759 1103 100-101 0.6382499237900383 1103 102-103 0.6616866075315961 1103 104-105 0.6747719957139218 1103 106-107 0.6674316326980367 1103 108-109 0.6171851537699702 1103 110-111 0.5954326855108008 1103 112-113 0.5858818763951348 1103 114-115 0.6410292630891732 1103 116-117 0.6013775598176885 1103 118-119 0.6980288653195323 1103 120-121 0.6790463945846064 1103 122-123 0.6756732040047524 1103 124-125 0.7313350590628218 1104 1 0.2835768144171098 1104 2 0.3218921623880959 1104 3 0.287071149552137 1104 4 0.2574921797683416 1104 5 0.3175686781406597 1104 6 0.19959548884958167 1104 7 0.21999738001841962 1104 8 0.3143662323884371 1104 9 0.3191803949807337 1104 10-11 0.3106268833304142 1104 12-13 0.3375143281586901 1104 14-15 0.476276431642944 1104 16-17 0.45140623701016835 1104 18-19 0.5690290204459174 1104 20-21 0.48848302370982566 1104 22-23 0.4348305435803894 1104 24-25 0.546201938254093 1104 26-27 0.5162822947384811 1104 28-29 0.5868762498654974 1104 30-31 0.4684788331548546 1104 32-33 -0.2516469185848891 1104 34-35 0.3925049069526594 1104 36-37 0.501413755024565 1104 38-39 0.5127028332117689 1104 40-41 0.5598322582853967 1104 42-43 0.5518782308528785 1104 44-45 0.5065702652499979 1104 46-47 0.4010025711377523 1104 48-49 0.4699398858572934 1104 50-51 0.5535983197722558 1104 52-53 0.49459825309970284 1104 54-55 0.48028773393473756 1104 56-57 0.4791802543647705 1104 58-59 0.5178934405143352 1104 60-61 0.46691424440068374 1104 62-63 0.5094301913598898 1104 64-65 0.5880744879138007 1104 66-67 0.4779492881264815 1104 68-69 0.5610699820461278 1104 70-71 0.49204663441574326 1104 72-73 0.4682642941775086 1104 74-75 0.5200238504531924 1104 76-77 0.5093123689323278 1104 78-79 0.42213621020589187 1104 80-81 0.4630973800898275 1104 82-83 0.4993949139430214 1104 84-85 0.5095649000227489 1104 86-87 0.4894575313731693 1104 88-89 0.4434461648733645 1104 90-91 0.4672184767631258 1104 92-93 0.45634776088375517 1104 94-95 0.4823140549831564 1104 96-97 0.46207661722319315 1104 98-99 0.5085784556961244 1104 100-101 0.4863650365045942 1104 102-103 0.502518069959411 1104 104-105 0.5165482904405678 1104 106-107 0.5455911619101741 1104 108-109 0.5228901399832964 1104 110-111 0.5061517235251998 1104 112-113 0.512934319777731 1104 114-115 0.5356788985127174 1104 116-117 0.5908058180946973 1104 118-119 0.6613980109860371 1104 120-121 0.5412141837945867 1104 122-123 0.6705135817475778 1104 124-125 0.8006838738891986 1105 1 0.19828377936100594 1105 2 0.2329369729486963 1105 3 0.17251693505689758 1105 4 0.19813372224688663 1105 5 0.20418470389503796 1105 6 0.2767796917192342 1105 7 0.20965341246973423 1105 8 0.20652816228626136 1105 9 0.18492123152145012 1105 10-11 0.19133162818825866 1105 12-13 0.24666641424322933 1105 14-15 0.39196979232839624 1105 16-17 0.4161014681988533 1105 18-19 0.362204144822158 1105 20-21 0.3215633405980398 1105 22-23 0.2944920486488769 1105 24-25 0.34984969390103515 1105 26-27 0.38206867110405085 1105 28-29 0.3734901514731419 1105 30-31 0.43209325772586027 1105 32-33 -0.05449204461655199 1105 34-35 0.42732888181279094 1105 36-37 0.4658387649633724 1105 38-39 0.42082941103737426 1105 40-41 0.4822313385847323 1105 42-43 0.3611629311020721 1105 44-45 0.4036574722118118 1105 46-47 0.36606568729706623 1105 48-49 0.365629976454116 1105 50-51 0.37571964633089294 1105 52-53 0.3373214907320019 1105 54-55 0.4843878466164071 1105 56-57 0.4255558564937374 1105 58-59 0.41785204708661894 1105 60-61 0.40006738581774925 1105 62-63 0.4500441826363044 1105 64-65 0.48148347222364407 1105 66-67 0.456848045806332 1105 68-69 0.47423357018927703 1105 70-71 0.4547997605953924 1105 72-73 0.40013785273624336 1105 74-75 0.3709575814644239 1105 76-77 0.3868139240272086 1105 78-79 0.4346196481318998 1105 80-81 0.42419680708753305 1105 82-83 0.4132599799906558 1105 84-85 0.3976970257120449 1105 86-87 0.3820450744361281 1105 88-89 0.38172418260395347 1105 90-91 0.37943826967214633 1105 92-93 0.4179818693190178 1105 94-95 0.3509263695161451 1105 96-97 0.34925221063610934 1105 98-99 0.3494208436948085 1105 100-101 0.3244655116237034 1105 102-103 0.3059476498705962 1105 104-105 0.3376608432542234 1105 106-107 0.35387978626571837 1105 108-109 0.4096606954531552 1105 110-111 0.38810520800372217 1105 112-113 0.31486829659427684 1105 114-115 0.39761935436871454 1105 116-117 0.47351125835584895 1105 118-119 0.472494624077175 1105 120-121 0.5233787191033912 1105 122-123 0.4982196114398576 1105 124-125 0.5358373423151299 1106 1 0.1449904471008594 1106 2 0.1362299849512567 1106 3 0.11200545976446108 1106 4 0.17023149202705667 1106 5 0.01533150464905475 1106 6 0.03873369207992994 1106 7 0.06159269564373204 1106 8 -0.012681906550596977 1106 9 0.00596383743353357 1106 10-11 0.02882671532108816 1106 12-13 0.07730478639628302 1106 14-15 0.11049188612774685 1106 16-17 0.046781882842132916 1106 18-19 0.16321664497746013 1106 20-21 0.2141758777643119 1106 22-23 0.1766888829759239 1106 24-25 0.1750497063409142 1106 26-27 0.1711801291565962 1106 28-29 0.23002493812254698 1106 30-31 0.23326781593822687 1106 32-33 0.12620515846974456 1106 34-35 0.3239271983073948 1106 36-37 0.2358034494259158 1106 38-39 0.2822772191982921 1106 40-41 0.31692803033926964 1106 42-43 0.1883171707839324 1106 44-45 0.12123422734494227 1106 46-47 0.1894339034985677 1106 48-49 0.21809849494019318 1106 50-51 0.1497366310956636 1106 52-53 0.1513552400169189 1106 54-55 0.12661065344806133 1106 56-57 0.18084529642803915 1106 58-59 0.2826180325512482 1106 60-61 0.20230408025319946 1106 62-63 0.06589474164785969 1106 64-65 0.0811179618515041 1106 66-67 0.17899684458185305 1106 68-69 0.16543595531346256 1106 70-71 0.16390481503957233 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90-91 -2.212427969797421 2216 92-93 -2.2927073480260667 2216 94-95 -2.230774752422306 2216 96-97 -2.085268642727982 2216 98-99 -2.103839500470947 2216 100-101 -2.2227103479918107 2216 102-103 -2.4054279088840396 2216 104-105 -2.2004724844701578 2216 106-107 -2.2367974904862464 2216 108-109 -2.228208499232313 2216 110-111 -2.249751649686818 2216 112-113 -2.3037287322528535 2216 114-115 -2.315898553891774 2216 116-117 -2.4371387233004533 2216 118-119 -2.609782453503108 2216 120-121 -2.5692072323357706 2216 122-123 -2.675659653469399 2216 124-125 -2.8243639569929044 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 51657.0 3 8508.0 4 4772.0 5 3952.0 6 4254.0 7 5030.0 8 5939.0 9 6878.0 10 7684.0 11 8189.0 12 8770.0 13 8931.0 14 8929.0 15 8057.0 16 7432.0 17 6909.0 18 6620.0 19 6527.0 20 6619.0 21 7291.0 22 8620.0 23 11061.0 24 14600.0 25 18914.0 26 23223.0 27 27096.0 28 32243.0 29 39808.0 30 47070.0 31 56056.0 32 71597.0 33 93432.0 34 125649.0 35 181376.0 36 326615.0 37 673457.0 38 354821.0 39 1976.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.94962479551246 19.036836265235145 12.801073641829927 24.21246529742247 2 15.178126622602056 20.26296678314318 40.990129316998555 23.568777277256206 3 18.91263145800755 24.924990769106632 29.756146547119656 26.406231225766163 4 11.942844488685322 16.316464549682188 38.73476448675587 33.00592647487661 5 13.103787618319219 38.65069605731246 35.174575664657546 13.070940659710772 6 31.682116066864275 36.94455109710853 18.209522388649134 13.163810447378063 7 28.0248418970511 32.69572932266165 21.613757995097085 17.665670785190166 8 25.415647149271386 36.394094229736325 20.42384424465609 17.766414376336197 9 26.248785509848886 16.065718947450648 20.36529932848678 37.32019621421368 10-11 24.35869553051599 26.961068214951855 28.841325274419958 19.838910980112193 12-13 24.789819966713583 24.51160645913613 28.708381280682005 21.99019229346828 14-15 21.908828307883518 26.335884897845258 26.188601607476862 25.56668518679436 16-17 22.00651197346938 29.444507467002936 26.748943652932304 21.800036906595373 18-19 21.740654133826993 28.656472213663935 28.21787110257916 21.38500254992991 20-21 22.75348051696117 27.893489005117033 27.950983752686565 21.402046725235234 22-23 22.18956565670532 28.092569073135127 27.623482089695383 22.094383180464174 24-25 21.919522050666686 28.051740683870108 27.997097086311207 22.031640179152 26-27 21.995747126764726 28.27399143499686 27.748823700210405 21.981437738028013 28-29 21.779358712202086 28.429040402987603 27.860553508927644 21.931047375882663 30-31 22.02988889588655 28.229016099574356 27.849145406145208 21.891949598393882 32-33 21.74080868637838 28.253814993131478 27.81205700216369 22.193319318326452 34-35 22.209419189480982 28.366783115503786 27.655933185285313 21.767864509729915 36-37 22.17932704381135 28.074969815338964 27.583856333076355 22.16184680777333 38-39 21.861933814143395 28.289746309621272 27.844111947736792 22.00420792849854 40-41 22.21178587778427 28.12491778089991 27.752235044016814 21.911061297299003 42-43 22.004553071437435 28.05848911880833 27.734441297085937 22.2025165126683 44-45 22.124605613208416 28.253268904032332 27.677641810394356 21.9444836723649 46-47 22.015626716689937 28.189274104821237 27.54083268658782 22.254266491901006 48-49 21.898416177049874 28.208309609180958 27.744866151914703 22.148408061854457 50-51 22.022820898880447 28.266354633979258 27.54290047423296 22.167923992907337 52-53 22.081052194388782 28.25373413838186 27.611506632791123 22.053707034438236 54-55 21.990698257770656 28.019552471785282 27.72224842462184 22.26750084582223 56-57 21.970657092503405 28.308563759319146 27.581885819903924 22.138893328273532 58-59 21.973762396861837 28.141024376018226 27.71485518448109 22.170358042638846 60-61 22.151581640338335 28.118337933035036 27.71636924462644 22.013711182000186 62-63 21.89685332895915 28.110087325050014 27.771716736230367 22.221342609760466 64-65 21.779513234048004 28.050730078753418 27.743409291464843 22.426347395733735 66-67 21.86089282361961 28.222138299234867 27.514663129619787 22.402305747525734 68-69 21.93672048821915 28.10098296567165 27.597590312822422 22.36470623328678 70-71 21.795795205662422 27.960469897544932 27.73246054030059 22.51127435649206 72-73 21.851271625887716 27.870261344433118 27.726256929321362 22.552210100357804 74-75 21.75352774669443 27.95336518237164 27.629969211036652 22.663137859897276 76-77 21.697680773797696 27.931821162582697 27.705087664583562 22.66541039903604 78-79 21.80453205843051 27.854229929975688 27.769167192368133 22.57207081922567 80-81 21.663354019284565 28.016837694607982 27.665533304059796 22.65427498204766 82-83 21.80618334380297 27.97044491860004 27.73766366849037 22.485708069106618 84-85 21.867629896413614 27.78421992624879 27.713684066462502 22.63446611087509 86-87 21.719365915942326 27.615074782215082 27.87846502918785 22.78709427265474 88-89 21.80611856168994 27.646103244560216 27.862832734706394 22.68494545904346 90-91 21.819346415376014 27.771963098017522 27.8620449501425 22.546645536463963 92-93 21.788488632812715 27.50954712160999 27.998136077319895 22.7038281682574 94-95 21.598639977862145 27.58548707448327 27.9544367148089 22.861436232845683 96-97 21.722494164674785 27.443524619744647 27.96176464268691 22.87221657289366 98-99 21.585571337647742 27.35869319367322 27.893705826555177 23.162029642123866 100-101 21.493016661519963 27.452967910635362 28.0596582484967 22.994357179347983 102-103 21.67525554054682 27.273217352125712 28.014988381133872 23.036538726193594 104-105 21.43634664209238 27.253746683950485 28.120779262048178 23.18912741190896 106-107 21.33521246150377 27.361082104328432 28.099000045802107 23.204705388365696 108-109 21.393769446370754 27.348687619286416 27.86767394008786 23.389868994254964 110-111 21.313605832752938 27.435799797042716 27.96974099379794 23.2808533764064 112-113 21.429085037099135 27.482099333517773 27.913990865409698 23.174824763973394 114-115 21.155539538728537 27.522798221518418 27.914479788876008 23.40718245087704 116-117 21.188940821474972 27.44194518721706 28.06040887650476 23.308705114803214 118-119 21.209376741999247 27.479477576755418 27.861768974332772 23.44937670691256 120-121 21.078198015507642 27.569515450302063 27.93478554032147 23.417500993868824 122-123 21.1850734752569 27.50889944937397 27.934757829761864 23.37126924560727 124-125 21.167008099016478 27.458307438183205 27.84869121252876 23.525993250271558 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 9172.0 1 4845.5 2 552.5 3 551.5 4 454.5 5 370.0 6 329.5 7 316.0 8 327.5 9 311.5 10 289.5 11 287.5 12 285.5 13 323.0 14 375.0 15 440.0 16 543.0 17 675.0 18 844.0 19 1119.0 20 1505.0 21 2070.5 22 3047.5 23 4250.5 24 5762.0 25 8036.5 26 11588.0 27 15963.5 28 20108.0 29 24697.5 30 29928.0 31 35211.0 32 40841.5 33 46655.0 34 53225.5 35 58591.0 36 64003.0 37 70536.5 38 74140.5 39 75942.0 40 78249.0 41 81112.0 42 83911.5 43 87544.5 44 89925.0 45 90953.0 46 91360.5 47 90834.0 48 90929.5 49 89159.5 50 85889.5 51 82143.5 52 77192.5 53 72717.5 54 67647.5 55 60878.0 56 54610.5 57 47922.5 58 41701.5 59 36390.0 60 29882.5 61 23530.0 62 18586.5 63 13975.0 64 10169.5 65 7712.0 66 5707.0 67 4392.0 68 3201.5 69 2096.5 70 1477.5 71 1116.0 72 937.5 73 804.0 74 636.0 75 504.0 76 335.0 77 160.0 78 95.0 79 68.5 80 35.5 81 22.0 82 21.0 83 15.0 84 12.5 85 9.5 86 6.0 87 8.0 88 8.0 89 6.5 90 5.5 91 5.0 92 5.5 93 4.5 94 2.0 95 2.0 96 1.0 97 2.0 98 3.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.45718037756672814 2 0.4485362107639959 3 0.4442577847707244 4 0.44168199769314254 5 0.4492347292935096 6 0.5071681098350536 7 0.502453109760836 8 0.5110536191554736 9 0.5163798229430157 10-11 0.535087022311555 12-13 0.5437748465223818 14-15 0.522142600811504 16-17 0.5167509109118199 18-19 0.5268576008857215 20-21 0.5279490360880866 22-23 0.5372698927162853 24-25 0.5309395685425673 26-27 0.5386232723672182 28-29 0.5187154942760772 30-31 0.5249148462255114 32-33 0.5261590823562078 34-35 0.5248493601133696 36-37 0.47344276208196934 38-39 0.44115810879600725 40-41 0.439630099512696 42-43 0.4515485719225238 44-45 0.45578334050770075 46-47 0.46086942855072244 48-49 0.47534185933408485 50-51 0.45661283126149826 52-53 0.45626357199674145 54-55 0.44689905796044815 56-57 0.4296107243549836 58-59 0.4473574607454415 60-61 0.443581094945258 62-63 0.45916678963503277 64-65 0.45988713686859384 66-67 0.466872322163731 68-69 0.47717547047405834 70-71 0.4728533870726922 72-73 0.48538306319584446 74-75 0.4736392204183952 76-77 0.47119440556509706 78-79 0.4832001927911142 80-81 0.47652060935263924 82-83 0.4782232482683289 84-85 0.47457785469242925 86-87 0.46604283140993347 88-89 0.46981919721011695 90-91 0.4546264191931936 92-93 0.4551066506822343 94-95 0.44988959041492876 96-97 0.4389752383912769 98-99 0.40225935818371217 100-101 0.4002292887073129 102-103 0.38977333946865445 104-105 0.39485942751167613 106-107 0.3932441034121757 108-109 0.40549000638271304 110-111 0.42725322431787477 112-113 0.4270131085733545 114-115 0.4203116964308322 116-117 0.4150291500513848 118-119 0.4581844979529041 120-121 0.45104651172943583 122-123 0.4157058398768512 124-125 0.42476475205648223 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2290562.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.91543175565951 #Duplication Level Percentage of deduplicated Percentage of total 1 83.56166774282447 60.92935081691012 2 10.216752296986627 14.899178097508118 3 3.085464593561046 6.749339490189124 4 1.2822338299093416 3.739785332782101 5 0.6398985579943577 2.332923981799126 6 0.3489010126810523 1.5264160787775456 7 0.20676771087672638 1.0553589838194115 8 0.14201678351510974 0.8284172069243404 9 0.09808166804324922 0.643650045541991 >10 0.3911606028734948 4.688179775188132 >50 0.019829415164689754 0.9688366836063509 >100 0.007107330764089637 0.8470520287511161 >500 0.0 0.0 >1k 0.0 0.0 >5k 1.1845480577260995E-4 0.7915114782024039 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 9164 0.4000764877789818 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 4.365740809460735E-5 0.0 3 0.0 0.0 0.0 4.365740809460735E-5 0.0 4 0.0 0.0 0.0 4.365740809460735E-5 0.0 5 0.0 0.0 0.0 4.365740809460735E-5 0.0 6 0.0 0.0 0.0 4.365740809460735E-5 0.0 7 0.0 0.0 0.0 4.365740809460735E-5 0.0 8 0.0 0.0 0.0 4.365740809460735E-5 0.0 9 0.0 0.0 0.0 4.365740809460735E-5 0.0 10-11 0.0 0.0 0.0 8.73148161892147E-5 0.0 12-13 0.0 0.0 0.0 1.3097222428382204E-4 0.0 14-15 0.0 0.0 0.0 1.3097222428382204E-4 0.0 16-17 0.0 0.0 0.0 2.619444485676441E-4 0.0 18-19 0.0 0.0 0.0 3.2743056070955515E-4 0.0 20-21 4.365740809460735E-5 0.0 0.0 4.365740809460735E-4 0.0 22-23 4.365740809460735E-5 0.0 0.0 4.8023148904068085E-4 0.0 24-25 8.73148161892147E-5 0.0 0.0 7.42175937608325E-4 0.0 26-27 8.73148161892147E-5 0.0 0.0 0.0012660648347436131 0.0 28-29 1.528009283311257E-4 0.0 0.0 0.002619444485676441 0.0 30-31 1.746296323784294E-4 0.0 0.0 0.00855685198654304 0.0 32-33 1.746296323784294E-4 0.0 0.0 0.021413958670404905 0.0 34-35 1.746296323784294E-4 0.0 0.0 0.04101613490488361 0.0 36-37 1.746296323784294E-4 0.0 0.0 0.07463233913773126 0.0 38-39 1.746296323784294E-4 0.0 0.0 0.1229610898984616 0.0 40-41 1.746296323784294E-4 0.0 0.0 0.1752844934998485 0.0 42-43 1.746296323784294E-4 0.0 0.0 0.2268220637555325 0.0 44-45 1.746296323784294E-4 0.0 0.0 0.2921117175610178 0.0 46-47 2.1828704047303674E-4 0.0 0.0 0.374864334604346 0.0 48-49 2.1828704047303674E-4 0.0 0.0 0.4644929934225749 0.0 50-51 2.1828704047303674E-4 0.0 0.0 0.5570248698790952 0.0 52-53 2.1828704047303674E-4 0.0 0.0 0.651826931556535 0.0 54-55 2.1828704047303674E-4 0.0 0.0 0.7609267943849588 0.0 56-57 2.1828704047303674E-4 0.0 0.0 0.8892795741831043 2.1828704047303674E-5 58-59 2.1828704047303674E-4 0.0 0.0 1.0317336967958082 4.365740809460735E-5 60-61 2.1828704047303674E-4 0.0 0.0 1.187503328877367 4.365740809460735E-5 62-63 2.1828704047303674E-4 0.0 0.0 1.3475950443602924 4.365740809460735E-5 64-65 2.1828704047303674E-4 0.0 0.0 1.5154577784840577 4.365740809460735E-5 66-67 2.1828704047303674E-4 0.0 0.0 1.7076813463246139 4.365740809460735E-5 68-69 2.1828704047303674E-4 0.0 0.0 1.9299412109342597 4.365740809460735E-5 70-71 2.1828704047303674E-4 0.0 0.0 2.176299964812129 4.365740809460735E-5 72-73 2.1828704047303674E-4 0.0 0.0 2.432219691062717 4.365740809460735E-5 74-75 2.1828704047303674E-4 0.0 0.0 2.6923086997863406 4.365740809460735E-5 76-77 2.1828704047303674E-4 0.0 0.0 2.9809933108119315 4.365740809460735E-5 78-79 2.1828704047303674E-4 0.0 0.0 3.309886394692656 4.365740809460735E-5 80-81 2.619444485676441E-4 0.0 0.0 3.677612743073534 4.365740809460735E-5 82-83 2.619444485676441E-4 0.0 0.0 4.064002633414857 4.365740809460735E-5 84-85 2.837731526149478E-4 0.0 0.0 4.442883449563906 4.365740809460735E-5 86-87 3.0560185666225146E-4 0.0 0.0 4.850381696718971 4.365740809460735E-5 88-89 3.0560185666225146E-4 0.0 0.0 5.307998648366645 4.365740809460735E-5 90-91 3.0560185666225146E-4 0.0 0.0 5.7979220820043285 4.365740809460735E-5 92-93 3.0560185666225146E-4 0.0 0.0 6.314585678099959 4.365740809460735E-5 94-95 3.0560185666225146E-4 0.0 0.0 6.842207283627337 4.365740809460735E-5 96-97 3.0560185666225146E-4 0.0 0.0 7.372339190120154 4.365740809460735E-5 98-99 3.0560185666225146E-4 0.0 0.0 7.953266490931047 4.365740809460735E-5 100-101 3.2743056070955515E-4 0.0 0.0 8.58162756563673 4.365740809460735E-5 102-103 3.492592647568588E-4 2.1828704047303674E-5 0.0 9.24190220565957 4.365740809460735E-5 104-105 5.238888971352882E-4 4.365740809460735E-5 0.0 9.90527652165713 4.365740809460735E-5 106-107 5.238888971352882E-4 4.365740809460735E-5 0.0 10.557496369886517 4.365740809460735E-5 108-109 5.238888971352882E-4 4.365740809460735E-5 0.0 11.232527213845335 4.365740809460735E-5 110-111 5.238888971352882E-4 4.365740809460735E-5 0.0 11.96522949389713 4.365740809460735E-5 112-113 5.238888971352882E-4 4.365740809460735E-5 0.0 12.75003689050984 4.365740809460735E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2540 0.0 48.0342 1 GTATCAA 4115 0.0 47.00499 1 ATCAACG 4560 0.0 40.984894 3 TCAACGC 4660 0.0 40.11067 4 CAACGCA 4665 0.0 40.07032 5 GTACATG 9390 0.0 37.58543 1 AACGCAG 5070 0.0 36.871864 6 TACATGG 9590 0.0 35.436268 2 TATCAAC 5450 0.0 34.94491 2 ACATGGG 9645 0.0 34.06402 3 ACGCAGA 6145 0.0 30.229757 7 CATGGGA 7065 0.0 29.995358 4 CGCAGAG 6115 0.0 29.79387 8 ATGGGAG 3430 0.0 23.604216 5 GCAGAGT 7600 0.0 23.345577 9 CATGGGG 3675 0.0 23.001024 4 TAGCCCT 2280 0.0 22.97545 4 GAGTACA 6875 0.0 22.42113 1 GGATACG 195 4.0330866E-5 21.364555 1 AGAGTAC 6390 0.0 21.290573 10-11 >>END_MODULE