FastQCFastQC Report
Thu 26 May 2016
SRR1364095_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364095_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2290562
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53260.23251935551187874No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52220.22797898507003958No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29610.12926958536813238No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44150.070.05781
GTATCAA76950.049.3344881
ATCAACG93350.040.342213
TCAACGC94950.039.725064
TATCAAC97450.038.767842
CAACGCA97300.038.7656145
AACGCAG101200.037.5076566
TGGTATC20650.035.1495322
GTGGTAT21300.034.1350141
GTACATG90000.033.440191
TACATGG90000.032.523882
ACGCAGA117900.032.049087
CGCAGAG118300.031.991018
ACATGGG89700.031.2391173
GCAGAGT134400.027.7160079
GAGTACT89800.027.00238612-13
CATGGGA63800.025.6437954
CAGAGTA129450.025.58107210-11
GTACTTT97050.024.79666514-15
AGAGTAC124050.024.27221310-11
Produced by FastQC (version 0.11.5)