FastQCFastQC Report
Thu 26 May 2016
SRR1364094_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364094_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3081677
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN123320.40017172468107465No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29550.049.1371541
GTATCAA46550.042.3141821
ATCAACG52250.036.569313
TCAACGC52450.036.211164
CAACGCA53800.035.4143375
TATCAAC57200.033.399212
AACGCAG56850.033.305986
TACATGG114500.031.7067382
GTACATG120150.030.8066351
ACATGGG114950.030.1378363
ACGCAGA66600.027.9840037
CGCAGAG68250.026.6095148
CATGGGG70350.025.8126744
AGTACAT80550.024.2345812
GAGTACA93850.022.8902531
CATGGGA52250.022.2200764
GTGGTAT19600.022.163811
TATGCCG2255.050275E-621.1699035
AGAGTAC71150.019.96015210-11
GCAGAGT87800.019.9385039
Produced by FastQC (version 0.11.5)