##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364094_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3081677 Sequences flagged as poor quality 0 Sequence length 125 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.694876198900793 31.0 31.0 34.0 27.0 34.0 2 30.73254659719367 31.0 31.0 34.0 27.0 34.0 3 30.840705563886157 31.0 31.0 34.0 27.0 34.0 4 34.3422029628673 37.0 35.0 37.0 30.0 37.0 5 34.36102128808438 37.0 35.0 37.0 30.0 37.0 6 34.398763400577025 37.0 35.0 37.0 30.0 37.0 7 34.31869530778209 37.0 35.0 37.0 30.0 37.0 8 34.26505438434982 37.0 35.0 37.0 30.0 37.0 9 35.77867537707554 39.0 37.0 39.0 30.0 39.0 10-11 35.68508639938579 39.0 36.0 39.0 30.0 39.0 12-13 35.588953839094756 39.0 35.0 39.0 30.0 39.0 14-15 36.80891556772498 40.0 37.0 41.0 30.0 41.0 16-17 36.795640328301765 40.0 37.0 41.0 30.0 41.0 18-19 36.77996590817273 40.0 37.0 41.0 30.0 41.0 20-21 36.78464923481598 40.0 37.0 41.0 30.0 41.0 22-23 36.72155599045584 40.0 37.0 41.0 30.0 41.0 24-25 36.70939913560052 40.0 37.0 41.0 30.0 41.0 26-27 36.64730291331635 40.0 37.0 41.0 30.0 41.0 28-29 36.59619372828496 40.0 36.5 41.0 30.0 41.0 30-31 36.538875910746 40.0 36.0 41.0 30.0 41.0 32-33 36.43192115851207 40.0 36.0 41.0 30.0 41.0 34-35 36.414606235501 40.0 36.0 41.0 30.0 41.0 36-37 36.345811874508584 40.0 36.0 41.0 29.0 41.0 38-39 36.25583748718636 40.0 36.0 41.0 29.0 41.0 40-41 36.164841415891416 40.0 36.0 41.0 29.0 41.0 42-43 36.042388770789415 40.0 35.0 41.0 28.0 41.0 44-45 35.933516393833614 40.0 35.0 41.0 28.0 41.0 46-47 35.810657476432475 39.5 35.0 41.0 28.0 41.0 48-49 35.682420967544616 39.0 35.0 41.0 27.5 41.0 50-51 35.52826545416667 39.0 35.0 41.0 27.0 41.0 52-53 35.36546919096323 39.0 35.0 41.0 27.0 41.0 54-55 35.16003104803002 39.0 34.5 41.0 26.0 41.0 56-57 34.947133168076995 38.0 34.0 40.5 26.0 41.0 58-59 34.71499446567567 38.0 34.0 40.0 26.0 41.0 60-61 34.451700810954556 37.5 34.0 40.0 26.0 41.0 62-63 34.17404322386804 37.0 33.5 40.0 26.0 41.0 64-65 33.87243682579323 36.5 33.0 40.0 25.0 41.0 66-67 33.56314565089073 36.0 33.0 39.0 24.5 41.0 68-69 33.244999232560716 35.5 33.0 39.0 23.5 41.0 70-71 32.90791929199588 35.0 33.0 38.5 23.5 40.5 72-73 32.56234592398879 35.0 32.0 37.5 23.0 40.0 74-75 32.31582511729815 35.0 32.0 37.0 23.0 39.0 76-77 32.117435896104624 35.0 32.0 37.0 23.0 39.0 78-79 31.840542990066773 35.0 32.0 36.0 22.5 39.0 80-81 31.673502446882004 35.0 32.0 36.0 22.5 37.5 82-83 31.615877653628203 35.0 32.0 35.5 23.0 37.0 84-85 31.437985551373487 35.0 32.0 35.0 23.0 37.0 86-87 31.269336630672193 35.0 32.0 35.0 23.0 36.0 88-89 31.110882483790483 35.0 32.0 35.0 21.5 36.0 90-91 30.98830523121015 35.0 32.0 35.0 20.0 36.0 92-93 30.885353331968275 35.0 32.0 35.0 20.0 36.0 94-95 30.79146695127361 35.0 32.0 35.0 20.0 35.0 96-97 30.71640003154127 35.0 32.0 35.0 19.5 35.0 98-99 30.64914411860815 35.0 32.0 35.0 19.0 35.0 100-101 30.574193693888102 35.0 32.0 35.0 18.5 35.0 102-103 29.437125792222872 33.5 29.5 34.5 16.0 35.0 104-105 30.305367499578963 34.5 31.5 35.0 17.0 35.0 106-107 30.422473867313155 35.0 32.0 35.0 17.0 35.0 108-109 30.400323427795968 35.0 32.0 35.0 15.5 35.0 110-111 30.334151664824056 35.0 32.0 35.0 11.5 35.0 112-113 30.26279262881866 35.0 32.0 35.0 7.5 35.0 114-115 30.161116171487148 35.0 32.0 35.0 3.5 35.0 116-117 30.06302315265357 35.0 32.0 35.0 2.0 35.0 118-119 29.92849607535118 35.0 32.0 35.0 2.0 35.0 120-121 29.791058407483977 35.0 32.0 35.0 2.0 35.0 122-123 29.615406968348726 35.0 31.5 35.0 2.0 35.0 124-125 28.725934450625424 34.5 29.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.40899197994562897 1101 2 0.5009404881566653 1101 3 0.5232922995404259 1101 4 0.513440354565347 1101 5 0.511119613401867 1101 6 0.467288697005678 1101 7 0.5015810638111162 1101 8 0.491996271557845 1101 9 0.5036683805385493 1101 10-11 0.5568422994154432 1101 12-13 0.6404197043962014 1101 14-15 0.7601098009468856 1101 16-17 0.7402737160053974 1101 18-19 0.6625677987081744 1101 20-21 0.693328528872776 1101 22-23 0.7891484800246218 1101 24-25 0.6608748120939936 1101 26-27 0.6152923635360565 1101 28-29 0.7662486744233021 1101 30-31 0.7641568628926336 1101 32-33 0.7090466401066635 1101 34-35 0.7036883751848251 1101 36-37 0.7568558598829256 1101 38-39 0.6896913396396513 1101 40-41 0.7229245562019244 1101 42-43 0.7917529623504436 1101 44-45 0.7285253316958133 1101 46-47 0.7209940500568024 1101 48-49 0.5617612670560561 1101 50-51 0.6221936255268474 1101 52-53 0.657909536720652 1101 54-55 0.6843167105044827 1101 56-57 0.6932515327743829 1101 58-59 0.576712410302747 1101 60-61 0.7169937732745595 1101 62-63 0.7389364837265262 1101 64-65 0.7229230951733783 1101 66-67 0.6832657048935147 1101 68-69 0.7067044306568988 1101 70-71 0.6820727354917366 1101 72-73 0.6903539150227402 1101 74-75 0.7396252462951765 1101 76-77 0.6717254588896537 1101 78-79 0.652447431531904 1101 80-81 0.6755196100432634 1101 82-83 0.6161206211274539 1101 84-85 0.5633068354569097 1101 86-87 0.5583052772406916 1101 88-89 0.6531117781253819 1101 90-91 0.6636129803820694 1101 92-93 0.6651892654112679 1101 94-95 0.6643798962822132 1101 96-97 0.6488732713371554 1101 98-99 0.6195992630324447 1101 100-101 0.68606902662777 1101 102-103 0.7304753683038356 1101 104-105 0.6454821827659138 1101 106-107 0.6535156601647998 1101 108-109 0.6762209862553021 1101 110-111 0.6762830527836812 1101 112-113 0.7542826392108495 1101 114-115 0.7619239222489007 1101 116-117 0.6917109389510046 1101 118-119 0.6988810458023877 1101 120-121 0.8062283543875033 1101 122-123 0.7937137345351459 1101 124-125 0.734293878913693 1102 1 0.620620175476521 1102 2 0.703879769921965 1102 3 0.6853148671367641 1102 4 0.6845758082179856 1102 5 0.7398309619862999 1102 6 0.6940860808310916 1102 7 0.6606394882000473 1102 8 0.6765258614814087 1102 9 0.7127320473494407 1102 10-11 0.7919222291807344 1102 12-13 0.8875458727809615 1102 14-15 0.9701071453969021 1102 16-17 0.9903226376460665 1102 18-19 0.8812745687581511 1102 20-21 0.9913355843943776 1102 22-23 0.951852974542966 1102 24-25 0.9533052126470807 1102 26-27 0.9562338704690987 1102 28-29 0.9668797109348262 1102 30-31 1.0204190389327081 1102 32-33 0.9405886218337329 1102 34-35 1.0277234376016935 1102 36-37 1.0317450936880732 1102 38-39 1.0640143003031781 1102 40-41 1.0464051352033863 1102 42-43 0.9512844638121436 1102 44-45 1.0145602979634631 1102 46-47 1.0858229616704875 1102 48-49 1.061365361181565 1102 50-51 1.0670449032813494 1102 52-53 1.057154376140005 1102 54-55 1.0221878064453804 1102 56-57 1.0525205697483386 1102 58-59 1.0909977811983822 1102 60-61 1.0242156330294634 1102 62-63 0.999196910766436 1102 64-65 0.8954109105485557 1102 66-67 0.9905376538153874 1102 68-69 1.0349017242851843 1102 70-71 0.9875155370054003 1102 72-73 1.0154568290310024 1102 74-75 1.0039738925369974 1102 76-77 1.0304031781384992 1102 78-79 1.051918221626707 1102 80-81 0.9772511202041763 1102 82-83 0.976132044570921 1102 84-85 0.956516319433188 1102 86-87 0.9281418464532507 1102 88-89 0.9450793346302149 1102 90-91 0.9130729422428772 1102 92-93 0.8831286044082525 1102 94-95 0.922008833535692 1102 96-97 0.985049554070816 1102 98-99 0.9447784325320789 1102 100-101 1.010628926449435 1102 102-103 1.0385661194353055 1102 104-105 0.9528538979535988 1102 106-107 0.9963067347697319 1102 108-109 1.038313033112022 1102 110-111 1.0908945526886775 1102 112-113 1.047319581116117 1102 114-115 1.1509942050420818 1102 116-117 1.1239895610868444 1102 118-119 1.1160057948414703 1102 120-121 1.2200404192836487 1102 122-123 1.1769960629946254 1102 124-125 1.2780092451621137 1103 1 0.23199199580171026 1103 2 0.2679062458168531 1103 3 0.2865153878066984 1103 4 0.2642610426739651 1103 5 0.28655202006051894 1103 6 0.23640571714970093 1103 7 0.239276314461776 1103 8 0.23252373542297278 1103 9 0.31179484864177454 1103 10-11 0.32431932508644223 1103 12-13 0.2815431303970044 1103 14-15 0.4976514514743329 1103 16-17 0.4792579754471262 1103 18-19 0.4497672070498311 1103 20-21 0.3901166304694854 1103 22-23 0.4469374174633174 1103 24-25 0.43886190524393953 1103 26-27 0.48797575610317523 1103 28-29 0.47475127142287477 1103 30-31 0.3861302201459935 1103 32-33 0.11884683083266623 1103 34-35 0.34456924844717207 1103 36-37 0.42583160234392636 1103 38-39 0.47233081086199746 1103 40-41 0.495879314272095 1103 42-43 0.4308441144306485 1103 44-45 0.45901403876830926 1103 46-47 0.41864334072369047 1103 48-49 0.4664765484675115 1103 50-51 0.41408568638701837 1103 52-53 0.3662630412543564 1103 54-55 0.4383791291004897 1103 56-57 0.47177551283196095 1103 58-59 0.48822222634955637 1103 60-61 0.4157779742753007 1103 62-63 0.5002100894673305 1103 64-65 0.4433153746209584 1103 66-67 0.445197609820454 1103 68-69 0.46914628980506734 1103 70-71 0.39360606384172314 1103 72-73 0.4620722838397242 1103 74-75 0.4606500474712476 1103 76-77 0.4581450878120421 1103 78-79 0.46269483170088677 1103 80-81 0.4613892594653102 1103 82-83 0.44934987061383325 1103 84-85 0.41224291506349786 1103 86-87 0.417344444026277 1103 88-89 0.44235401600737134 1103 90-91 0.4182069401212125 1103 92-93 0.4411042858769427 1103 94-95 0.4539090612783312 1103 96-97 0.4822459277517339 1103 98-99 0.5036360174285441 1103 100-101 0.4857831188331474 1103 102-103 0.3918042211616708 1103 104-105 0.502280069829844 1103 106-107 0.49679718941587225 1103 108-109 0.5033498576873967 1103 110-111 0.4604523640181668 1103 112-113 0.487766129632643 1103 114-115 0.5671219307251114 1103 116-117 0.6049575168689181 1103 118-119 0.5874906033950751 1103 120-121 0.5890811352043599 1103 122-123 0.5970604322797257 1103 124-125 0.6577062661373176 1104 1 0.23590233818845618 1104 2 0.29045958903741464 1104 3 0.2925887819524853 1104 4 0.37377806462099983 1104 5 0.34873592074503534 1104 6 0.29632608826304363 1104 7 0.3347196059843469 1104 8 0.32326552103101847 1104 9 0.3248063283420848 1104 10-11 0.3407929667305183 1104 12-13 0.36565137349438714 1104 14-15 0.3923536816406781 1104 16-17 0.4057774443244142 1104 18-19 0.5190954639159315 1104 20-21 0.4937563399712843 1104 22-23 0.45535005240264326 1104 24-25 0.45799981884819374 1104 26-27 0.4425723116637883 1104 28-29 0.5880498847761402 1104 30-31 0.4962730891853866 1104 32-33 -0.3185753407654346 1104 34-35 0.417485992302673 1104 36-37 0.4811800345195465 1104 38-39 0.5599983856222579 1104 40-41 0.6315230594709504 1104 42-43 0.6569800385918683 1104 44-45 0.6566935923539461 1104 46-47 0.6434421777922523 1104 48-49 0.610318299530249 1104 50-51 0.6173790958894116 1104 52-53 0.5486169717452398 1104 54-55 0.6596256476633897 1104 56-57 0.65515689290838 1104 58-59 0.6441524329968615 1104 60-61 0.61589054206474 1104 62-63 0.6509827364957417 1104 64-65 0.6470788200342454 1104 66-67 0.6973734749264793 1104 68-69 0.6339442588300273 1104 70-71 0.5848992335735446 1104 72-73 0.5890920951707983 1104 74-75 0.5481195997830426 1104 76-77 0.6714219250916287 1104 78-79 0.5695043559388893 1104 80-81 0.5503964134149619 1104 82-83 0.5132021308264889 1104 84-85 0.5733081862971368 1104 86-87 0.5632877163177952 1104 88-89 0.5656256917796725 1104 90-91 0.560699893441992 1104 92-93 0.6154093172835324 1104 94-95 0.5766717318039092 1104 96-97 0.6107995694946098 1104 98-99 0.6309378106090371 1104 100-101 0.5861398106554319 1104 102-103 0.6256154504349212 1104 104-105 0.6700480227183618 1104 106-107 0.63359914209056 1104 108-109 0.5390347864390144 1104 110-111 0.631022205536695 1104 112-113 0.5855008269236102 1104 114-115 0.6015691916064405 1104 116-117 0.5640847704742136 1104 118-119 0.656147890779966 1104 120-121 0.6131311479250812 1104 122-123 0.7197330785671312 1104 124-125 0.7920013679718885 1105 1 0.22917263594085924 1105 2 0.28961468441353944 1105 3 0.27838046074949574 1105 4 0.24748222775404827 1105 5 0.2950412298261256 1105 6 0.2581424116937967 1105 7 0.14274219162454926 1105 8 0.19936199837997748 1105 9 0.281129716905383 1105 10-11 0.25534315258843776 1105 12-13 0.3122433278083534 1105 14-15 0.42520554591587967 1105 16-17 0.444713465741323 1105 18-19 0.49853743658363214 1105 20-21 0.45123026501210717 1105 22-23 0.3736292633186835 1105 24-25 0.5056512266006479 1105 26-27 0.5009545882861985 1105 28-29 0.4613131363826213 1105 30-31 0.43782699642097356 1105 32-33 0.04116521760998637 1105 34-35 0.42671375369569375 1105 36-37 0.5364735571283958 1105 38-39 0.5372748287109133 1105 40-41 0.6720655599667182 1105 42-43 0.6829824794274444 1105 44-45 0.6044619260459143 1105 46-47 0.5299694299219198 1105 48-49 0.4888809279536446 1105 50-51 0.5880397564550464 1105 52-53 0.6296405299223551 1105 54-55 0.6009377391873443 1105 56-57 0.5637049332781601 1105 58-59 0.5909089380240999 1105 60-61 0.6297320523027494 1105 62-63 0.6269330168648466 1105 64-65 0.5786874298338702 1105 66-67 0.6103997207652441 1105 68-69 0.6757625547597144 1105 70-71 0.6475687946450606 1105 72-73 0.5897141707037932 1105 74-75 0.5892715180646277 1105 76-77 0.6424828869116368 1105 78-79 0.5696180119603866 1105 80-81 0.5031186729072168 1105 82-83 0.456778376876219 1105 84-85 0.4581237418245614 1105 86-87 0.44492143064931966 1105 88-89 0.4266230589682607 1105 90-91 0.466817118948196 1105 92-93 0.5098502984277111 1105 94-95 0.5169598643220823 1105 96-97 0.4841461512699823 1105 98-99 0.5265042764394501 1105 100-101 0.5242154744487912 1105 102-103 0.5711963671933589 1105 104-105 0.5765795257414581 1105 106-107 0.5809645374974863 1105 108-109 0.6159242716484385 1105 110-111 0.5775150074978015 1105 112-113 0.6184030584844358 1105 114-115 0.6461075846632447 1105 116-117 0.6883593233392951 1105 118-119 0.6310531158979984 1105 120-121 0.7297928569741075 1105 122-123 0.6100315482454945 1105 124-125 0.6512764904355919 1106 1 0.1563026819666824 1106 2 0.21108742279608705 1106 3 0.22224517912157893 1106 4 0.21245741249475003 1106 5 0.13421931502884377 1106 6 0.16809036339085992 1106 7 0.2728595640644471 1106 8 0.20840903967134494 1106 9 0.27644550152036373 1106 10-11 0.286969530176016 1106 12-13 0.3085669768164081 1106 14-15 0.34652482093225245 1106 16-17 0.3401577446041699 1106 18-19 0.3784159099506468 1106 20-21 0.4319222348909122 1106 22-23 0.44490820529625097 1106 24-25 0.4464031575311367 1106 26-27 0.4039446182227593 1106 28-29 0.5099648259742793 1106 30-31 0.5205673791342562 1106 32-33 0.4185067538261933 1106 34-35 0.41105424806665525 1106 36-37 0.45160618358951155 1106 38-39 0.5458141792500797 1106 40-41 0.5536721776622571 1106 42-43 0.5497876411772253 1106 44-45 0.5586947850539232 1106 46-47 0.4857269217564095 1106 48-49 0.49319739834018606 1106 50-51 0.41153664190920836 1106 52-53 0.43183680125930124 1106 54-55 0.4135071625677611 1106 56-57 0.4484696828616421 1106 58-59 0.511235294950616 1106 60-61 0.4988301747833148 1106 62-63 0.47701113327897104 1106 64-65 0.4953455463359049 1106 66-67 0.4523936675124389 1106 68-69 0.5221854867848066 1106 70-71 0.5225878511816475 1106 72-73 0.48199721851877086 1106 74-75 0.5478761048213272 1106 76-77 0.47786406160315664 1106 78-79 0.46759408468733454 1106 80-81 0.41389150668234365 1106 82-83 0.38076229201638156 1106 84-85 0.38325166459613413 1106 86-87 0.45026159092644136 1106 88-89 0.44932187777071064 1106 90-91 0.39326956992212203 1106 92-93 0.49613561456400035 1106 94-95 0.45506671720210434 1106 96-97 0.5066743184575735 1106 98-99 0.4819845583091471 1106 100-101 0.482291511568377 1106 102-103 0.42970353922090965 1106 104-105 0.5438563312768636 1106 106-107 0.5489961533247438 1106 108-109 0.5093803624867945 1106 110-111 0.5307969299440707 1106 112-113 0.521796572668034 1106 114-115 0.5820348269384255 1106 116-117 0.6347836181155593 1106 118-119 0.6285117388159591 1106 120-121 0.6125775071207116 1106 122-123 0.6330905289955382 1106 124-125 0.6473868249250678 1107 1 0.26905997325703623 1107 2 0.2111939681074979 1107 3 0.23725728409243274 1107 4 0.2986355308053348 1107 5 0.2319545801250129 1107 6 0.17309669895467295 1107 7 0.191188053612116 1107 8 0.1184737972214478 1107 9 0.14229611162167544 1107 10-11 0.23657996040123663 1107 12-13 0.2911223388601769 1107 14-15 0.2122902379201932 1107 16-17 0.1637365024023083 1107 18-19 0.2489296028564283 1107 20-21 0.2188583562317845 1107 22-23 0.28386205543186094 1107 24-25 0.21104833899786257 1107 26-27 0.22770756568881012 1107 28-29 0.2774703392627913 1107 30-31 0.24304682225167795 1107 32-33 0.2686969285799137 1107 34-35 0.23967234028010864 1107 36-37 0.2224768248564004 1107 38-39 0.33026352439541995 1107 40-41 0.32581737260890264 1107 42-43 0.2699876832345822 1107 44-45 0.370411426288463 1107 46-47 0.3468690486725734 1107 48-49 0.36426926789766867 1107 50-51 0.37040066183793385 1107 52-53 0.35662929132459453 1107 54-55 0.31164326665432895 1107 56-57 0.3421976364475654 1107 58-59 0.35221986852673126 1107 60-61 0.3132328793950663 1107 62-63 0.31389479075951243 1107 64-65 0.3329869911679779 1107 66-67 0.3226068120549641 1107 68-69 0.26065955950090824 1107 70-71 0.25110133919260846 1107 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70-71 -2.8277612458048935 2216 72-73 -2.84150381325297 2216 74-75 -2.951360408447968 2216 76-77 -2.9243541380221423 2216 78-79 -2.9099276166275274 2216 80-81 -2.7799024167058626 2216 82-83 -2.6483404685516696 2216 84-85 -2.6773916639701802 2216 86-87 -2.7408025348449527 2216 88-89 -2.746296198577962 2216 90-91 -2.8399147919751684 2216 92-93 -2.873949168569027 2216 94-95 -2.8659447358297427 2216 96-97 -2.7601123033487447 2216 98-99 -2.8394788278024983 2216 100-101 -2.841047311554032 2216 102-103 -3.0208810015005767 2216 104-105 -2.9180688061911937 2216 106-107 -2.9394794313256156 2216 108-109 -2.931123414991589 2216 110-111 -3.0646921444823647 2216 112-113 -3.0644003191043474 2216 114-115 -3.115092194274414 2216 116-117 -3.1608838663347747 2216 118-119 -3.313869570888542 2216 120-121 -3.3657328478721134 2216 122-123 -3.37805362912988 2216 124-125 -3.6018943456668815 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 71801.0 3 12389.0 4 7210.0 5 5976.0 6 6282.0 7 7360.0 8 8653.0 9 9521.0 10 10442.0 11 10654.0 12 11449.0 13 11457.0 14 11044.0 15 10238.0 16 9228.0 17 8457.0 18 8285.0 19 8570.0 20 9067.0 21 9805.0 22 11917.0 23 15354.0 24 20228.0 25 26537.0 26 32041.0 27 38458.0 28 46376.0 29 55730.0 30 67466.0 31 81534.0 32 102769.0 33 134141.0 34 183597.0 35 272198.0 36 518516.0 37 880687.0 38 344709.0 39 1531.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.80365163429673 17.55187676973019 12.50079053109998 25.143681064873103 2 16.181325030420435 19.14944037154492 39.08686212020905 25.5823724778256 3 19.290523593640042 22.887188401337806 28.829706822893936 28.992581182128212 4 12.99218693724746 15.103940037815342 35.93034311180297 35.973529913134236 5 14.625146521364412 37.04552299814982 33.75884832577746 14.570482154708305 6 34.61072938318587 34.55828369529447 16.798308169948818 14.032678751570844 7 30.28829470531295 31.305590561247786 20.14357986907436 18.262534864364902 8 27.06189753347915 34.75057225553811 19.294780259744325 18.892749951238414 9 27.047605006054027 15.418654231556095 19.001653114692495 38.53208764769738 10-11 25.9885375368346 25.349236474676793 27.331849482378523 21.33037650611008 12-13 25.906905056090512 22.611393895575045 27.547694796370713 23.93400625196373 14-15 23.054549499429314 24.648201119454228 24.57441460907665 27.722834772039807 16-17 23.8327767302793 27.650399027063187 25.180382934855345 23.33644130780217 18-19 23.602557312737126 26.619879050074807 26.405002669236353 23.37256096795171 20-21 24.324537245073966 26.181477733041152 26.106970458877054 23.387014563007824 22-23 24.028519807070932 25.86491180907539 26.110966837706773 23.9956015461469 24-25 23.782643580536913 26.227347084511894 26.287959837689616 23.70204949726158 26-27 23.751357202700834 26.496234415006487 26.10023137696044 23.652177005332238 28-29 23.496448546367546 26.460568027082736 26.387909645265633 23.65507378128408 30-31 23.902387467609582 26.194259815232513 26.064950953807802 23.838401763350102 32-33 23.61191848611478 26.26914457395066 26.26668167242431 23.85225526751025 34-35 24.03862260188791 26.372419417317378 25.79927827274766 23.789679708047053 36-37 24.24803961700863 26.26292908875631 25.77104213423478 23.71798916000028 38-39 23.67225110412151 26.39844526653738 25.992258926679813 23.9370447026613 40-41 23.93871994852859 26.140188539462883 26.260099455332615 23.660992056675916 42-43 24.019452206261057 26.14901144953066 25.9940757382346 23.83746060597368 44-45 23.724091955222033 26.349011272729644 26.044080349982725 23.882816422065602 46-47 23.916701793437632 26.486563317349432 25.710514330907547 23.88622055830539 48-49 24.032511487463967 26.268707375419748 25.97012949623399 23.728651640882298 50-51 23.724708010113535 26.205644072901958 26.06999933497893 23.999648582005573 52-53 23.9524858290726 26.15722720557266 25.96802071230833 23.922266253046406 54-55 23.890123999450434 25.949643018550457 25.992099095253675 24.16813388674544 56-57 23.657007336606394 26.46862082456853 25.95132839620119 23.923043442623886 58-59 23.900701660372388 26.104236625719636 26.08344041102186 23.91162130288611 60-61 23.845074949119084 26.069574842859133 26.21268352657868 23.872666681443103 62-63 23.71393679856507 26.18221513420541 26.114993437571517 23.988854629658004 64-65 23.650547598439765 26.17267892100532 26.041545435430002 24.135228045124915 66-67 23.609537358217565 26.484137956441998 25.756905182128513 24.149419503211924 68-69 23.4609279883977 25.959625403247106 26.37454523638578 24.204901371969413 70-71 23.57074830530828 25.727427123609875 26.299975514145878 24.40184905693597 72-73 23.53679071538879 26.10615400267123 26.0079067365082 24.349148545431778 74-75 23.29462424349525 26.326854394395166 25.92391229924625 24.454609062863337 76-77 23.54902681585101 26.17934236405621 25.936052440031332 24.335578380061442 78-79 23.538325089099676 26.25662337492949 26.083445557080857 24.121605978889978 80-81 23.527740268200674 26.263496722931844 26.12587013116587 24.082892877701607 82-83 23.605238446138497 26.22493429933419 26.093500361924267 24.07632689260305 84-85 23.62296274716717 26.110484464034162 25.822257602105754 24.44429518669292 86-87 23.42539714520353 25.821243824839136 26.359627545659432 24.393731484297902 88-89 23.4637916093118 25.979781961304944 26.244750032766444 24.311676396616807 90-91 23.439165849116172 26.25053441641727 26.190911229093377 24.119388505373177 92-93 23.41064049342624 25.75518444887822 26.290356520048253 24.543818537647287 94-95 23.43542093819981 25.740777563001377 26.4293188256041 24.39448267319471 96-97 23.37148504189235 25.766858468199562 26.411980436113268 24.44967605379482 98-99 23.152517484237674 25.618026729204995 26.39840913026557 24.83104665629176 100-101 23.07116262279529 25.688227030211095 26.528946325850285 24.711664021143335 102-103 23.075817975874365 25.327924953765553 26.64270326460632 24.95355380575376 104-105 23.02604359081115 25.510743282557097 26.51186853039505 24.95134459623671 106-107 23.12828323139197 25.499067460479058 26.51982766343875 24.852821644690227 108-109 23.00378750791949 25.515021413780314 26.44641374365728 25.034777334642914 110-111 22.772471636395753 25.77657109094245 26.606336670189613 24.844620602472187 112-113 23.014991413435386 25.613252135071818 26.619458349825067 24.752298101667726 114-115 22.7608245023973 25.802360832714445 26.453279490123222 24.983535174765038 116-117 22.602013916631854 25.607524782931435 26.90659616025047 24.883865140186245 118-119 22.655391229399285 25.729280626102653 26.532527581715026 25.082800562783035 120-121 22.664073451890385 25.882817355198007 26.58383613133993 24.869273061571683 122-123 22.635458625933154 25.873864732245195 26.556324742584657 24.934351899236987 124-125 22.72242806739222 25.91996876664588 26.4518385036147 24.905764662347206 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 12065.0 1 6318.5 2 554.5 3 453.5 4 336.0 5 300.5 6 266.0 7 222.0 8 204.0 9 210.5 10 207.0 11 205.0 12 243.5 13 262.5 14 276.0 15 320.0 16 373.0 17 451.0 18 545.5 19 679.0 20 899.5 21 1253.0 22 1824.5 23 2805.0 24 4033.0 25 5812.5 26 9180.0 27 13470.5 28 16924.5 29 20633.0 30 25232.5 31 30601.5 32 37117.0 33 42882.0 34 48538.0 35 53629.5 36 59608.5 37 64911.5 38 68924.5 39 72639.5 40 75746.0 41 82035.5 42 87771.5 43 92791.0 44 99828.5 45 106355.0 46 109492.5 47 111307.0 48 113740.5 49 114855.0 50 113856.5 51 113028.5 52 116070.5 53 120470.0 54 125666.0 55 123349.0 56 112379.0 57 102929.5 58 97161.0 59 89059.5 60 77826.5 61 67228.0 62 55772.5 63 43761.5 64 33274.5 65 25018.5 66 18021.0 67 15603.0 68 11981.5 69 7273.5 70 5413.5 71 3658.5 72 2799.5 73 2188.5 74 1537.0 75 1198.0 76 766.5 77 361.5 78 189.0 79 141.5 80 100.0 81 62.5 82 37.0 83 27.5 84 22.0 85 19.0 86 18.5 87 14.0 88 8.5 89 8.0 90 8.5 91 8.0 92 10.0 93 11.0 94 10.0 95 6.5 96 4.5 97 5.5 98 4.5 99 2.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.45815963191470105 2 0.4490412200889321 3 0.4435247431836627 4 0.4416426510630413 5 0.44991736642094554 6 0.5077754741979773 7 0.5032649430813158 8 0.5107284118355039 9 0.517542883306719 10-11 0.5339462896338585 12-13 0.5425617285653234 14-15 0.5216640160535968 16-17 0.5161962139445503 18-19 0.5254768750910624 20-21 0.5258338235966975 22-23 0.5357148072299595 24-25 0.5294357585171969 26-27 0.5370128017959053 28-29 0.5179160567444284 30-31 0.5240166312043735 32-33 0.5252659509740962 34-35 0.5229133358233196 36-37 0.47410874014375937 38-39 0.44219429875356825 40-41 0.44021485704050106 42-43 0.45277295446602617 44-45 0.4574457349034308 46-47 0.4613072687371194 48-49 0.47500111140784707 50-51 0.45770533381662 52-53 0.4584841305561875 54-55 0.447824350183358 56-57 0.43146961865244154 58-59 0.4481650737569187 60-61 0.44550418489672994 62-63 0.4612748188729708 64-65 0.460528471997552 66-67 0.4683651141894494 68-69 0.47698055312091436 70-71 0.4737680165701986 72-73 0.48431422241850786 74-75 0.47412496507583374 76-77 0.4722915477514353 78-79 0.48308112758085936 80-81 0.4764451303624617 82-83 0.47821364795856286 84-85 0.47534183498140786 86-87 0.4655906508047404 88-89 0.4710097781175639 90-91 0.4568454124166809 92-93 0.4564235641827485 94-95 0.45063126343221566 96-97 0.44050690581783875 98-99 0.4027839387450404 100-101 0.40046377345841244 102-103 0.3892685703271303 104-105 0.3936493019871972 106-107 0.3927082559268866 108-109 0.40589912570331027 110-111 0.4289872040450703 112-113 0.4290034289771446 114-115 0.42230253203044965 116-117 0.41636420689124787 118-119 0.4564073392506742 120-121 0.4519454829302357 122-123 0.4170618789704437 124-125 0.42712133685652326 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 3081677.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.74346081938377 #Duplication Level Percentage of deduplicated Percentage of total 1 82.08795625292746 53.96746335657625 2 10.133106080591455 13.323709251760476 3 3.375554503967873 6.65761905825919 4 1.536604018923962 4.040866644521405 5 0.8209761664711228 2.698690721702108 6 0.4801282932139426 1.8939177379913064 7 0.31328969500626275 1.441772415213265 8 0.22798186843705007 1.1990653628096886 9 0.17605560046321983 1.0417054023978098 >10 0.7851971345078649 9.07576830243476 >50 0.04884166224512703 2.1843265393389664 >100 0.014062023350491806 1.3919967426317104 >500 0.0 0.0 >1k 1.2334994709053553E-4 0.08304079271546556 >5k 0.0 0.0 >10k+ 1.2334994709053553E-4 1.0000576716475795 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 12332 0.40017172468107465 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.244986414864374E-5 2 0.0 0.0 0.0 0.0 3.244986414864374E-5 3 0.0 0.0 0.0 0.0 3.244986414864374E-5 4 0.0 0.0 0.0 3.244986414864374E-5 3.244986414864374E-5 5 0.0 0.0 0.0 3.244986414864374E-5 3.244986414864374E-5 6 0.0 0.0 0.0 3.244986414864374E-5 3.244986414864374E-5 7 0.0 0.0 0.0 3.244986414864374E-5 6.489972829728748E-5 8 0.0 0.0 0.0 3.244986414864374E-5 6.489972829728748E-5 9 0.0 0.0 0.0 3.244986414864374E-5 6.489972829728748E-5 10-11 0.0 0.0 0.0 3.244986414864374E-5 6.489972829728748E-5 12-13 0.0 0.0 0.0 3.244986414864374E-5 6.489972829728748E-5 14-15 0.0 0.0 0.0 3.244986414864374E-5 6.489972829728748E-5 16-17 0.0 0.0 0.0 3.244986414864374E-5 6.489972829728748E-5 18-19 0.0 0.0 0.0 8.112466037160936E-5 1.9469918489186245E-4 20-21 0.0 0.0 0.0 9.734959244593123E-5 3.244986414864374E-4 22-23 0.0 0.0 0.0 2.1092411696618433E-4 3.244986414864374E-4 24-25 0.0 0.0 0.0 3.7317343770940304E-4 3.244986414864374E-4 26-27 0.0 0.0 0.0 7.138970112701623E-4 3.244986414864374E-4 28-29 0.0 0.0 0.0 0.001979441713067268 3.5694850563508117E-4 30-31 0.0 0.0 0.0 0.006441298033505783 3.5694850563508117E-4 32-33 0.0 0.0 0.0 0.016435856191288057 3.5694850563508117E-4 34-35 0.0 0.0 0.0 0.03251476387694103 3.5694850563508117E-4 36-37 0.0 0.0 0.0 0.05823128121474119 3.5694850563508117E-4 38-39 0.0 0.0 0.0 0.09811216425342435 3.5694850563508117E-4 40-41 0.0 0.0 0.0 0.14360687378982287 3.7317343770940304E-4 42-43 0.0 0.0 0.0 0.18751153998293785 3.893983697837249E-4 44-45 0.0 0.0 0.0 0.2450451491184832 4.2184823393236865E-4 46-47 0.0 3.244986414864374E-5 0.0 0.3167917987511345 4.2184823393236865E-4 48-49 0.0 3.244986414864374E-5 0.0 0.3961317165945685 4.2184823393236865E-4 50-51 0.0 3.244986414864374E-5 0.0 0.48282152866767025 4.380731660066905E-4 52-53 0.0 3.244986414864374E-5 0.0 0.5679699721937115 4.542980980810124E-4 54-55 0.0 3.244986414864374E-5 0.0 0.6619934535644066 4.542980980810124E-4 56-57 0.0 3.244986414864374E-5 0.0 0.7770768967675716 4.8674796222965614E-4 58-59 0.0 3.244986414864374E-5 0.0 0.9113544346146595 5.191978263782999E-4 60-61 0.0 3.244986414864374E-5 0.0 1.0556914303478269 5.191978263782999E-4 62-63 0.0 3.244986414864374E-5 0.0 1.1985681821943053 5.191978263782999E-4 64-65 0.0 3.244986414864374E-5 0.0 1.352753711696586 5.191978263782999E-4 66-67 0.0 3.244986414864374E-5 0.0 1.5375394630910377 5.354227584526217E-4 68-69 0.0 3.244986414864374E-5 0.0 1.7487394039024855 5.840975546755874E-4 70-71 0.0 3.244986414864374E-5 0.0 1.9836439704745177 6.165474188242311E-4 72-73 0.0 3.244986414864374E-5 0.0 2.222393845948164 6.32772350898553E-4 74-75 0.0 3.244986414864374E-5 0.0 2.4655731278781 6.652222150471967E-4 76-77 0.0 3.244986414864374E-5 0.0 2.7454045313639295 6.814471471215186E-4 78-79 0.0 3.244986414864374E-5 0.0 3.064402920877172 6.814471471215186E-4 80-81 0.0 3.244986414864374E-5 0.0 3.416224347976767 7.138970112701623E-4 82-83 0.0 3.244986414864374E-5 0.0 3.779889975490618 7.463468754188061E-4 84-85 0.0 3.244986414864374E-5 0.0 4.147741635479643 7.62571807493128E-4 86-87 0.0 3.244986414864374E-5 0.0 4.540482341270678 7.950216716417716E-4 88-89 0.0 3.244986414864374E-5 0.0 4.970832439609992 8.599213999390591E-4 90-91 0.0 3.244986414864374E-5 0.0 5.451398702719331 9.085961961620248E-4 92-93 0.0 3.244986414864374E-5 0.0 5.952392155310242 9.248211282363466E-4 94-95 0.0 3.244986414864374E-5 0.0 6.463185466874043 9.410460603106685E-4 96-97 0.0 3.244986414864374E-5 0.0 6.982610442301383 9.410460603106685E-4 98-99 0.0 3.244986414864374E-5 0.0 7.546686430797257 9.410460603106685E-4 100-101 0.0 3.244986414864374E-5 0.0 8.169821172043662 9.410460603106685E-4 102-103 0.0 3.244986414864374E-5 0.0 8.813658926616904 9.572709923849905E-4 104-105 0.0 3.244986414864374E-5 0.0 9.468610759661054 9.734959244593123E-4 106-107 0.0 3.244986414864374E-5 0.0 10.110225698540113 9.734959244593123E-4 108-109 0.0 3.244986414864374E-5 0.0 10.793165539412469 9.734959244593123E-4 110-111 0.0 3.244986414864374E-5 0.0 11.524747726643643 9.734959244593123E-4 112-113 0.0 3.244986414864374E-5 0.0 12.286784111378317 9.734959244593123E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2955 0.0 49.137154 1 GTATCAA 4655 0.0 42.314182 1 ATCAACG 5225 0.0 36.56931 3 TCAACGC 5245 0.0 36.21116 4 CAACGCA 5380 0.0 35.414337 5 TATCAAC 5720 0.0 33.39921 2 AACGCAG 5685 0.0 33.30598 6 TACATGG 11450 0.0 31.706738 2 GTACATG 12015 0.0 30.806635 1 ACATGGG 11495 0.0 30.137836 3 ACGCAGA 6660 0.0 27.984003 7 CGCAGAG 6825 0.0 26.609514 8 CATGGGG 7035 0.0 25.812674 4 AGTACAT 8055 0.0 24.234581 2 GAGTACA 9385 0.0 22.890253 1 CATGGGA 5225 0.0 22.220076 4 GTGGTAT 1960 0.0 22.16381 1 TATGCCG 225 5.050275E-6 21.169903 5 AGAGTAC 7115 0.0 19.960152 10-11 GCAGAGT 8780 0.0 19.938503 9 >>END_MODULE