FastQCFastQC Report
Thu 26 May 2016
SRR1364094_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364094_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3081677
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37320.12110289300273844No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37190.12068104476880606No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39900.065.406751
GTATCAA65000.049.5912741
TATCAAC86550.036.2949752
ATCAACG86750.036.21133
TCAACGC86600.036.136624
CAACGCA88650.035.3009725
AACGCAG93550.033.5161066
GTACATG116850.030.3395841
TACATGG114750.030.2270342
GTGGTAT20450.029.4272331
ACATGGG113800.029.172363
ACGCAGA110350.028.307987
CGCAGAG113200.027.4901568
TGGTATC23000.026.1260192
CATGGGA54650.023.7326354
CATGGGG69300.023.351534
AGTACAT78200.023.0523682
GCAGAGT135250.022.788439
AGAGTAC120650.022.31583810-11
GAGTACT76050.021.90671512-13
Produced by FastQC (version 0.11.5)