FastQCFastQC Report
Thu 26 May 2016
SRR1364093_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364093_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2704117
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN110750.4095606809912441No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29750.055.016631
GTATCAA48200.050.1334081
ATCAACG56150.040.4958273
TCAACGC56300.040.0752224
CAACGCA57000.039.792695
GTACATG117050.038.4413451
AACGCAG59300.037.8477336
TACATGG120250.036.775622
TATCAAC63300.036.7645572
ACATGGG118850.035.1087573
ACGCAGA70750.031.8078777
CGCAGAG70600.031.2851728
CATGGGA65650.030.287044
GAGTACA77700.027.0397031
CATGGGG57800.026.2637924
GTATAGG10350.024.727281
GCAGAGT84650.024.6155999
ATGGGAT31700.023.6627085
TAAGGTG15400.022.8078585
AGTACAT92200.022.0130252
Produced by FastQC (version 0.11.5)