FastQCFastQC Report
Thu 26 May 2016
SRR1364093_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364093_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2704117
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60020.22195785167579657No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57480.2125647669830854No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33260.12299763656676097No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48250.068.804961
GTATCAA85000.053.641931
ATCAACG110850.040.5751653
TCAACGC111800.040.2303854
CAACGCA113050.039.7855575
TATCAAC115500.039.044642
AACGCAG117850.038.518486
GTACATG110350.034.4055821
TGGTATC22800.033.400222
TACATGG112950.033.1839832
ACGCAGA136600.033.063257
CGCAGAG138150.032.6922958
ACATGGG111650.032.025063
GTGGTAT24300.031.3953651
GCAGAGT157750.028.2908749
CATGGGA59800.027.3593444
GAGTACT102350.025.32041712-13
AGAGTAC144050.025.21964310-11
CAGAGTA149900.024.9102810-11
TAAGGTG17800.024.399285
Produced by FastQC (version 0.11.5)