FastQCFastQC Report
Thu 26 May 2016
SRR1364092_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364092_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2238858
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN91240.4075291956881589No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20850.047.097141
GTATCAA31800.044.7288171
ATCAACG34300.040.257753
TCAACGC35400.039.008564
CAACGCA35650.038.7376175
AACGCAG38200.035.9967086
GTACATG73500.034.6555751
TATCAAC42050.033.5427782
TACATGG76050.033.024022
ACATGGG78950.030.9091743
ACGCAGA46450.029.0933257
CGCAGAG48650.027.4105918
GAGTACA57100.026.6821331
AGTACAT54150.026.3772472
CATGGGG51350.024.3418754
GCAGAGT58850.021.4458189
AGAGTAC49100.020.00646210-11
CAGAGTA54000.019.45892110-11
CATGGGA36500.018.7534084
TAAGACG2301.4329422E-418.1152954
Produced by FastQC (version 0.11.5)